LOCUS       Exported               11000 bp DNA     circular SYN 04-DEC-2007
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 6, 2023 from SnapGene 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             367..817
                     /codon_start=1
                     /label=GAL1 promoter
                     /note="GAL1 promoter"
                     /translation="TD*KPPSG*QPSEGRLSSVRPRLHRSRS*NADVPRAALLRTIKIL
                     QY*LLWL*RGKIGSNLAPQTFK*TNQINNHRMIMRLVF*PYFWGN*SAKR*FLIY*QIY
                     KCKNCITTLTNTFNIFGLYYFLFKCNKSINKKLLIYLYTLTSRRKN"
     misc_feature    832..2995
                     /note="hADAR2aHISTEV , full length human ADAR2a with His
                     tag and TEV protease site"
     misc_feature    832..891
                     /label=His and TEV protease site
                     /note="His and TEV protease site"
     misc_feature    868..894
     CDS             1126..1467
                     /codon_start=1
                     /label=RBM 1
                     /note="RBM 1"
                     /translation="PKNALMQLNEIKPGLQYTLLSQTGPVHAPLFVMSVEVNGQVFEGS
                     GPTKKKAKLHAAEKALRSFVQFPNASEAHLAMGRTLSVNTDFTSDQADFPDTLFNGFET
                     PDKAEPPFYV"
     CDS             1594..1803
                     /codon_start=1
                     /label=RBM 2
                     /note="RBM 2"
                     /translation="NPVMILNELRPGLKYDFLSESGESHAKSFVMSVVVDGQFFEGSGR
                     NKKLAKARAAQSALAAIFNLHLDQT"
     CDS             1840..2994
                     /codon_start=1
                     /label=CATALYTIC DOMAIN
                     /note="CATALYTIC DOMAIN"
                     /translation="LHLPQVLADAVSRLVLGKFGDLTDNFSSPHARRKVLAGVVMTTGT
                     DVKDAKVISVSTGTKCINGEYMSDRGLALNDCHAEIISRRSLLRFLYTQLELYLNNKDD
                     QKRSIFQKSERGGFRLKENVQFHLYISTSPCGDARIFSPHEPILEEPADRHPNRKARGQ
                     LRTKIESGEGTIPVRSNASIQTWDGVLQGERLLTMSCSDKIARWNVVGIQGSLLSIFVE
                     PIYFSSIILGSLYHGDHLSRAMYQRISNIEDLPPLYTLNKPLLSGISNAEARQPGKAPN
                     FSVNWTVGDSAIEVINATTGKDELGRASRLCKHALYCRWMRVHGKVPSHLLRSKITKPN
                     VYHESKLAAKEYQAAKARLFTAFIKAGLGAWVEKPTEQDQFSLTP"
ORIGIN
        1 caattcgaca ggttatcagc aacaacacag tcatatccat tctcaattag ctctaccaca
       61 gtgtgtgaac caatgtatcc agcaccacct gtaaccaaaa caattttaga agtactttca
      121 ctttgtaact gagctgtcat ttatattgaa ttttcaaaaa ttcttacttt ttttttggat
      181 ggacgcaaag aagtttaata atcatattac atggcattac caccatatac atatccatat
      241 acatatccat atctaatctt acttatatgt tgtggaaatg taaagagccc cattatctta
      301 gcctaaaaaa accttctctt tggaactttc agtaatacgc ttaactgctc attgctatat
      361 tgaagtacgg attagaagcc gccgagcggg tgacagccct ccgaaggaag actctcctcc
      421 gtgcgtcctc gtcttcaccg gtcgcgttcc tgaaacgcag atgtgcctcg cgccgcactg
      481 ctccgaacaa taaagattct acaatactag cttttatggt tatgaagagg aaaaattggc
      541 agtaacctgg ccccacaaac cttcaaatga acgaatcaaa ttaacaacca taggatgata
      601 atgcgattag ttttttagcc ttatttctgg ggtaattaat cagcgaagcg atgatttttg
      661 atctattaac agatatataa atgcaaaaac tgcataacca ctttaactaa tactttcaac
      721 attttcggtt tgtattactt cttattcaaa tgtaataaaa gtatcaacaa aaaattgtta
      781 atatacctct atactttaac gtcaaggaga aaaaaccccg gatccgtaac catgtcacac
      841 catcaccatc accatcacca tcaccatcac catgagaacc tctatttcca gggagatata
      901 gaagatgaag aaaacatgag ttccagcagc actgatgtga aggaaaaccg caatctggac
      961 aacgtgtccc ccaaggatgg cagcacacct gggcctggcg agggctctca gctctccaat
     1021 gggggtggtg gtggccccgg cagaaagcgg cccctggagg agggcagcaa tggccactcc
     1081 aagtaccgcc tgaagaaaag gaggaaaaca ccagggcccg tcctccccaa gaacgccctg
     1141 atgcagctga atgagatcaa gcctggtttg cagtacacac tcctgtccca gactgggccc
     1201 gtgcacgcgc ctttgtttgt catgtctgtg gaggtgaatg gccaggtttt tgagggctct
     1261 ggtcccacaa agaaaaaggc aaaactccat gctgctgaga aggccttgag gtctttcgtt
     1321 cagtttccta atgcctctga ggcccacctg gccatgggga ggaccctgtc tgtcaacacg
     1381 gacttcacat ctgaccaggc cgacttccct gacacgctct tcaatggttt tgaaactcct
     1441 gacaaggcgg agcctccctt ttacgtgggc tccaatgggg atgactcctt cagttccagc
     1501 ggggacctca gcttgtctgc ttccccggtg cctgccagcc tagcccagcc tcctctccct
     1561 gtcttaccac cattcccacc cccgagtggg aagaatcccg tgatgatctt gaacgaactg
     1621 cgcccaggac tcaagtatga cttcctctcc gagagcgggg agagccatgc caagagcttc
     1681 gtcatgtctg tggtcgtgga tggtcagttc tttgaaggct cggggagaaa caagaagctt
     1741 gccaaggccc gggctgcgca gtctgccctg gccgccattt ttaacttgca cttggatcag
     1801 acgccatctc gccagcctat tcccagtgag ggtcttcagc tgcatttacc gcaggtttta
     1861 gctgacgctg tctcacgcct ggtcctgggt aagtttggtg acctgaccga caacttctcc
     1921 tcccctcacg ctcgcagaaa agtgctggct ggagtcgtca tgacaacagg cacagatgtt
     1981 aaagatgcca aggtgataag tgtttctaca ggaacaaaat gtattaatgg tgaatacatg
     2041 agtgatcgtg gccttgcatt aaatgactgc catgcagaaa taatatctcg gagatccttg
     2101 ctcagatttc tttatacaca acttgagctt tacttaaata acaaagatga tcaaaaaaga
     2161 tccatctttc agaaatcaga gcgagggggg tttaggctga aggagaatgt ccagtttcat
     2221 ctgtacatca gcacctctcc ctgtggagat gccagaatct tctcaccaca tgagccaatc
     2281 ctggaagaac cagcagatag acacccaaat cgtaaagcaa gaggacagct acggaccaaa
     2341 atagagtctg gtgaggggac gattccagtg cgctccaatg cgagcatcca aacgtgggac
     2401 ggggtgctgc aaggggagcg gctgctcacc atgtcctgca gtgacaagat tgcacgctgg
     2461 aacgtggtgg gcatccaggg atccctgctc agcattttcg tggagcccat ttacttctcg
     2521 agcatcatcc tgggcagcct ttaccacggg gaccaccttt ccagggccat gtaccagcgg
     2581 atctccaaca tagaggacct gccacctctc tacaccctca acaagccttt gctcagtggc
     2641 atcagcaatg cagaagcacg gcagccaggg aaggccccca acttcagtgt caactggacg
     2701 gtaggcgact ccgctattga ggtcatcaac gccacgactg ggaaggatga gctgggccgc
     2761 gcgtcccgcc tgtgtaagca cgcgttgtac tgtcgctgga tgcgtgtgca cggcaaggtt
     2821 ccctcccact tactacgctc caagattacc aaacccaacg tgtaccatga gtccaagctg
     2881 gcggcaaagg agtaccaggc cgccaaggcg cgtctgttca cagccttcat caaggcgggg
     2941 ctgggggcct gggtggagaa gcccaccgag caggaccagt tctcactcac gccctgactc
     3001 gagcgagtaa tgactagatt atataatcag aaatagcctt tggtgatcac ttccatttta
     3061 gtcagcataa tagaaagttt aattacgcgc ttttttacat tccgacaaag atctgcccga
     3121 gaaaattctt cgagaagaca acaacaacaa aaaaaataaa gcctggggcg caaaacatcg
     3181 tgcgtttaca taatatcttg aaaagcaaac agatatataa accgctattg aaattacctt
     3241 ttaagtattc tattcgccta gaagccttgt ttcaagtagc atcaacaaca ggtgttaggt
     3301 taaactgctt tgactttttg ttctttaacg aatatcatta aaaaaaaaga catctataaa
     3361 gaatgtctcc aaactcgtcg aaaactcgaa cggaccagat aagctccatg cctggtatta
     3421 atgtaagaat gttgataagg atgacttagt tcctaataaa atctgttgaa accaatggta
     3481 catcaatagt aagagaaaaa aagatccaca ggacgggtgt ggtcgccatg atcgcgtagt
     3541 cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca
     3601 gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc
     3661 catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg
     3721 gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg
     3781 cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac
     3841 cgcattaaag ctttttcttt ccaatttttt ttttttcgtc attataaaaa tcattacgac
     3901 cgagattccc gggtaataac tgatataatt aaattgaagc tctaatttgt gagtttagta
     3961 tacatgcatt tacttataat acagtttttt agttttgctg gccgcatctt ctcaaatatg
     4021 cttcccagcc tgcttttctg taacgttcac cctctacctt agcatccctt ccctttgcaa
     4081 atagtcctct tccaacaata ataatgtcag atcctgtaga gaccacatca tccacggttc
     4141 tatactgttg acccaatgcg tctcccttgt catctaaacc cacaccgggt gtcataatca
     4201 accaatcgta accttcatct cttccaccca tgtctctttg agcaataaag ccgataacaa
     4261 aatctttgtc gctcttcgca atgtcaacag tacccttagt atattctcca gtagataggg
     4321 agcccttgca tgacaattct gctaacatca aaaggcctct aggttccttt gttacttctt
     4381 ctgccgcctg cttcaaaccg ctaacaatac ctgggcccac cacaccgtgt gcattcgtaa
     4441 tgtctgccca ttctgctatt ctgtatacac ccgcagagta ctgcaatttg actgtattac
     4501 caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt gtacttggcg gataatgcct
     4561 ttagcggctt aactgtgccc tccatggaaa aatcagtcaa gatatccaca tgtgttttta
     4621 gtaaacaaat tttgggacct aatgcttcaa ctaactccag taattccttg gtggtacgaa
     4681 catccaatga agcacacaag tttgtttgct tttcgtgcat gatattaaat agcttggcag
     4741 caacaggact aggatgagta gcagcacgtt ccttatatgt agctttcgac atgatttatc
     4801 ttcgtttcct gcaggttttt gttctgtgca gttgggttaa gaatactggg caatttcatg
     4861 tttcttcaac actacatatg cgtatatata ccaatctaag tctgtgctcc ttccttcgtt
     4921 cttccttctg ttcggagatt accgaatcaa aaaaatttca aagaaaccga aatcaaaaaa
     4981 aagaataaaa aaaaaatgat gaattgaatt gaaaagctta tcgatgataa gctgtcaaac
     5041 atgagaattc cacggactat agactatact agtatactcc gtctactgta cgatacactt
     5101 ccgctcaggt ccttgtcctt taacgaggcc ttaccactct tttgttactc tattgatcca
     5161 gctcagcaaa ggcagtgtga tctaagattc tatcttcgcg atgtagtaaa actagctaga
     5221 ccgagaaaga gactagaaat gcaaaaggca cttctacaat ggctgccatc attattatcc
     5281 gatgtgacgc tgcagcttct caatgatatt cgaatacgct ttgaggagat acagcctaat
     5341 atccgacaaa ctgttttaca gatttacgat cgtacttgtt acccatcatt gaattttgaa
     5401 catccgaacc tgggagtttt ccctgaaaca gatagtatat ttgaacctgt ataataatat
     5461 atagtctagc gctttacgga agacaatgta tgtatttcgg ttcctggaga aactattgca
     5521 tctattgcat aggtaatctt gcacgtcgca tccccggttc attttctgcg tttccatctt
     5581 gcacttcaat agcatatctt tgttaacgaa gcatctgtgc ttcattttgt agaacaaaaa
     5641 tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag
     5701 aaatgcaacg cgaaagcgct attttaccaa cgaagaatct gtgcttcatt tttgtaaaac
     5761 aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag
     5821 aacagaaatg caacgcgaga gcgctatttt accaacaaag aatctatact tcttttttgt
     5881 tctacaaaaa tgcatcccga gagcgctatt tttctaacaa agcatcttag attacttttt
     5941 ttctcctttg tgcgctctat aatgcagtct cttgataact ttttgcactg taggtccgtt
     6001 aaggttagaa gaaggctact ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc
     6061 acttcccgcg tttactgatt actagcgaag ctgcgggtgc attttttcaa gataaaggca
     6121 tccccgatta tattctatac cgatgtggat tgcgcatact ttgtgaacag aaagtgatag
     6181 cgttgatgat tcttcattgg tcagaaaatt atgaacggtt tcttctattt tgtctctata
     6241 tactacgtat aggaaatgtt tacattttcg tattgttttc gattcactct atgaatagtt
     6301 cttactacaa tttttttgtc taaagagtaa tactagagat aaacataaaa aatgtagagg
     6361 tcgagtttag atgcaagttc aaggagcgaa aggtggatgg gtaggttata tagggatata
     6421 gcacagagat atatagcaaa gagatacttt tgagcaatgt ttgtggaagc ggtattcgca
     6481 atattttagt agctcgttac agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag
     6541 agcgcttttg gttttcaaaa gcgctctgaa gttcctatac tttctagaga ataggaactt
     6601 cggaatagga acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct
     6661 gcgcacatac agctcactgt tcacgtcgca cctatatctg cgtgttgcct gtatatatat
     6721 atacatgaga agaacggcat agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta
     6781 tttatgtagg atgaaaggta gtctagtacc tcctgtgata ttatcccatt ccatgcgggg
     6841 tatcgtatgc ttccttcagc actacccttt agctgttcta tatgctgcca ctcctcaatt
     6901 ggattagtct catccttcaa tgctatcatt tcctttgata ttggatcata tgcatagtac
     6961 cgagaaacta gtgcgaagta gtgatcaggt attgctgtta tctgatgagt atacgttgtc
     7021 ctggccacgg cagaagcacg cttatcgctc caatttccca caacattagt caactccgtt
     7081 aggcccttca ttgaaagaaa tgaggtcatc aaatgtcttc caatgtgaga ttttgggcca
     7141 ttttttatag caaagattga ataaggcgca tttttcttca aagctttatt gtacgatctg
     7201 actaagttat cttttaataa ttggtattcc tgtttattgc ttgaagaatt gccggtccta
     7261 tttactcgtt ttaggactgg ttcagaattc ttgaagacga aagggcctcg tgatacgcct
     7321 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     7381 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     7441 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     7501 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     7561 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     7621 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     7681 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
     7741 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     7801 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     7861 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     7921 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     7981 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc
     8041 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     8101 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     8161 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     8221 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     8281 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     8341 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     8401 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     8461 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8521 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8581 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8641 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     8701 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8761 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8821 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8881 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8941 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     9001 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     9061 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     9121 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     9181 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     9241 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     9301 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     9361 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     9421 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     9481 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     9541 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca
     9601 gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt
     9661 ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt
     9721 tcctgtttgg tcacttgatg cctccgtgta agggggaatt tctgttcatg ggggtaatga
     9781 taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt
     9841 tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa
     9901 tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc
     9961 agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt
    10021 ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg
    10081 ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag
    10141 taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc
    10201 gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg
    10261 cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat
    10321 tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt
    10381 cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc
    10441 ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt
    10501 gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag
    10561 ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc
    10621 gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa
    10681 cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc
    10741 gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc
    10801 gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa
    10861 aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat
    10921 aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
    10981 tctcaagggc atcggtcgac
//
