LOCUS       Exported                9895 bp ds-DNA     circular SYN 27-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9895)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 4, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9895
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        9..376
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(37..56)
                     /label=Seq 4
     protein_bind    53..71
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(353..388)
                     /label=MDM4 Dox Hifi 2F
     primer_bind     366..403
                     /label=Fragment 2.FOR
     primer_bind     complement(367..405)
                     /label=Fragment 1.FOR
     protein_bind    383..386
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             386..1105
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1083..1125)
                     /label=Fragment 2.REV
     primer_bind     1086..1117
                     /label=Fragment 1.REV
     primer_bind     1106..1117
                     /label=MDM4 Dox HiFI 2R
     polyA_signal    1298..1432
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1322..1340)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    1476..1591
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     primer_bind     complement(1769..1793)
                     /label=Seq 20
     misc_feature    2083..2316
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     primer_bind     complement(2368..2387)
                     /label=Seq 19
     misc_feature    2813..2938
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     primer_bind     2966..2984
                     /label=Seq 18
     primer_bind     complement(2966..2984)
                     /label=Seq 17
     LTR             2985..3618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3610..3634)
                     /label=Seq 16
     polyA_signal    3786..3920
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3810..3829)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3921..3940)
                     /label=Seq 15
     promoter        3968..4072
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4073..4933
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4291..4310)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(4481..4500)
                     /label=Seq 14
     primer_bind     complement(5065..5084)
                     /label=Seq 13
     rep_origin      5104..5692
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5593..5612
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(5609..5630)
                     /label=Seq 12
     primer_bind     5846..5863
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5980..6001
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6016..6046
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6054..6070
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6059..6081
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6078..6094
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6078..6094
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6207..6229
                     /label=Seq 11
     primer_bind     complement(6207..6229)
                     /label=Seq 10
     LTR             6222..6855
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6928..6947)
                     /label=Seq 9
     misc_feature    7062..7650
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7168..7179
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7522..7539)
                     /label=Seq 8
     primer_bind     7577..7597
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7664..8263)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7747..7767)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(8110..8129)
                     /label=Seq 7
     primer_bind     8244..8263
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(8272..8601)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8286..8421
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8325..8336
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(8340..8359)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             complement(8612..9358)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(8707..8728)
                     /label=Seq 6
     primer_bind     complement(9316..9334)
                     /label=Seq 5
     promoter        complement(9377..9887)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9440..9458)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag ctttgcttat gtaaaccagg gcgcctataa aagagtgctg
      301 attttttgag taaacttcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac
      361 ttcctaccct cgtaaagaat tcggcatggt gagcaagggc gaggagctgt tcaccggggt
      421 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
      481 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
      541 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
      601 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
      661 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
      721 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
      781 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
      841 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
      901 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
      961 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     1021 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     1081 cggcatggac gagctgtaca agtagaccgg tgccggcgga tccagaccac ctcccctgcg
     1141 agctaagctg gacagccaat gacgggtaag agagtgacat ttttcactaa cctaagacag
     1201 gagggccgtc agagctactg cctaatccaa agacgggtaa aagtgataaa aatgtatcac
     1261 tccaacctaa gacaggcgca gcttccgagg gatttgagat ccagacatga taagatacat
     1321 tgatgagttt ggacaaacca aaactagaat gcagtgaaaa aaatgcctta tttgtgaaat
     1381 ttgtgatgct attgccttat ttgtaaccat tataagctgc aataaacaag ttaagtcgat
     1441 aaactggatc tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt
     1501 tttgtaattc tttagtttgt atgtctgttg ctattatgtc tactattctt tcccctgcac
     1561 tgtacccccc aatcccccct tttcttttaa aggcgatacc gtcgagatcc gttcactaat
     1621 cgaatggatc tgtctctgtc tctctctcca ccttcttctt ctattccttc gggcctgtcg
     1681 ggtcccctcg gggttgggag gtgggtctga aacgataatg gtgaatatcc ctgcctaact
     1741 ctattcacta tagaaagtac agcaaaaact attcttaaac ctaccaagcc tcctactatc
     1801 attatgaata attttatata ccacagccaa tttgttatgt taaaccaatt ccacaaactt
     1861 gcccatttat ctaattccaa taattcttgt tcattctttt cttgctggtt ttgcgattct
     1921 tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca ctccatccag
     1981 gtcgtgtgat tccaaatctg ttccagagat ttattactcc aactagcatt ccaaggcaca
     2041 gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg ttgatccttt
     2101 aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca gactgtgagt
     2161 tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg ctgcactata
     2221 ccagacaata attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt
     2281 cctgctgctc ccaagaaccc aaggaacaaa gctcctattc ccactgctct tttttctctc
     2341 tgcaccactc ttctctttgc cttggtgggt gctactccta atggttcaat ttttactact
     2401 ttatatttat ataattcact tctccaattg tccctcatat ctcctcctcc aggtctgaag
     2461 atcagcggcc ggccgcttgc tgtgcggtgg tcttactttt gttttgctct tcctctatct
     2521 tgtctaaagc ttccttggtg tcttttatct ctatcctttg atgcacacaa tagagggttg
     2581 ctactgtatt atataatgat ctaagttctt ctgatcctgt ctgaagggat ggttgtagct
     2641 gtcccagtat ttgtctacag ccttctgatg tttctaacag gccaggatta actgcgaatc
     2701 gttctagctc cctgcttgcc catactatat gttttaattt atattttttc tttccccctg
     2761 gccttaaccg aattttttcc catcgcgatc taattctccc ccgcttaata ctgacgctct
     2821 cgcacccatc tctctccttc tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc
     2881 gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct
     2941 cctctggttt ccctttcgct ttcaagtccc tgttcgggcg ccactgctag agattttcca
     3001 cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca
     3061 cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta
     3121 gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac aggcaaaaag
     3181 cagctgctta tatgcaggat ctgagggctc gccactcccc agtcccgccc aggccacgcc
     3241 tccctggaaa gtccccagcg gaaagtccct tgtagcaagc tcgatatcag cagttcttga
     3301 agtactccgg atgcagctct cgggccacgt gatgaaatgc taggcggctg tcaaacctcc
     3361 actctaacac ttctctctcc gggtcatcca tcccatgcag gctcacaggg tgtaacaagc
     3421 tggtgttctc tcctttattg gcctcttcta ccttatctgg ctcaactggt actagcttgt
     3481 agcaccatcc aaaggtcagt ggatatctga cccctggccc tggtgtgtag ttctgctaat
     3541 cagggaagta gccttgtgtg tggtagatcc acagatcaag gatatcttgt cttctttggg
     3601 agtgaattag cccttccaac tactaagttt gtagtacata tttaacaaat acaatttctt
     3661 taaaatgaaa ataattcaga ggaatcacag gtttagagta aatgaaacca caggtaattg
     3721 gcagtggtaa tagggtatgg ggtgggaagt ttgggatgat tttggttagc ttgagttatc
     3781 cagttgatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     3841 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     3901 taagctgcaa taaacaagtt gatctcccga tccgtcgacg tcaggtggca cttttcgggg
     3961 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4021 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4081 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4141 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4201 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4261 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     4321 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     4381 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     4441 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     4501 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     4561 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     4621 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     4681 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     4741 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     4801 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     4861 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     4921 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     4981 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5041 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5101 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5161 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5221 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     5281 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5341 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5401 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5461 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5521 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5581 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5641 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5701 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     5761 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     5821 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     5881 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     5941 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     6001 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     6061 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tctagctaga
     6121 ggtcgacggt aaacgctagc aatgtcaagg cctctcactc tctgatattc atttctttgc
     6181 aagttataaa tactgaataa taagatgaca tgaactacta ctgctagaga ttttccacac
     6241 tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac
     6301 tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc
     6361 agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacagg caaaaagcag
     6421 ctgcttatat gcaggatctg agggctcgcc actccccagt cccgcccagg ccacgcctcc
     6481 ctggaaagtc cccagcggaa agtcccttgt agcaagctcg atatcagcag ttcttgaagt
     6541 actccggatg cagctctcgg gccacgtgat gaaatgctag gcggctgtca aacctccact
     6601 ctaacacttc tctctccggg tcatccatcc catgcaggct cacagggtgt aacaagctgg
     6661 tgttctctcc tttattggcc tcttctacct tatctggctc aactggtact agcttgtagc
     6721 accatccaaa ggtcagtgga tatctgaccc ctggccctgg tgtgtagttc tgctaatcag
     6781 ggaagtagcc ttgtgtgtgg tagatccaca gatcaaggat atcttgtctt cgttgggagt
     6841 gaattagccc ttccagtccc ctcttttctt ttaaaaagtg gctaagatct acagctgcct
     6901 tgtaagtcat tggtcttaaa ggtacctgag gtgtgactgg aaaacccacc tcctcctcct
     6961 cttgtgcttc tagccaggca caatcagcat tggtagctgc tgtattgcta cttgtgattg
     7021 ctccatgttt ttctaggtct cgactgcaga attaattcca ggcggggagg cggcccaaag
     7081 ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag
     7141 caggccgcgg gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt
     7201 cccgcgcaga atccaggtgg caacacaggc gagcagccat ggaaaggacg tcagcttccc
     7261 cgacaacacc acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag
     7321 gcaggcggcg atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag
     7381 gagctgacag gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca
     7441 caccacgcca cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga
     7501 tttatacaag gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat
     7561 taaagcagcg tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct
     7621 ttcacaaatt ttgtaatcca gaggttgatt gttccagacg cgttcaggca ccgggcttgc
     7681 gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac gtcggcggtg acggtgaagc
     7741 cgagccgctc gtagaagggg aggttgcggg gcgcggaggt ctccaggaag gcgggcaccc
     7801 cggcgcgctc ggccgcctcc actccgggga gcacgacggc gctgcccaga cccttgccct
     7861 ggtggtcggg cgagacgccg acggtggcca ggaaccacgc gggctccttg ggccggtgcg
     7921 gcgccaggag gccttccatc tgttgctgcg cggccagccg ggaaccgctc aactcggcca
     7981 tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac gctctccggc gtggtccaga
     8041 ccgccaccgc ggcgccgtcg tccgcgaccc acaccttgcc gatgtcgagc ccgacgcgcg
     8101 tgaggaagag ttcttgcagc tcggtgaccc gctcgatgtg gcggtccggg tcgacggtgt
     8161 ggcgcgtggc ggggtagtcg gcgaacgcgg cggcgagggt gcgtacggcc cgggggacgt
     8221 cgtcgcgggt ggcgaggcgc accgtgggct tgtactcggt catggtcgcg ttttgcaaaa
     8281 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
     8341 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
     8401 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
     8461 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt
     8521 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     8581 tccacaccct aactgacaca cattcgcgcg tttacccggg gagcatgtca aggtcaaaat
     8641 cgtcaagagc gtcagcaggc agcatatcaa ggtcaaagtc gtcaagggca tcggctggga
     8701 gcatgtctaa gtcaaaatcg tcaagggcgt cggtcggccc gccgctttcg cactttagct
     8761 gtttctccag gccacatatg attagttcca ggccgaaaag gaaggcaggt tcggctccct
     8821 gccggtcgaa cagctcaatt gcttgtttca gaagtggggg catagaatcg gtggtaggtg
     8881 tctctctttc ctcttttgct acttgatgct cctgttcctc caatacgcag cccagtgtaa
     8941 agtggcccac ggcggacaga gcgtacagtg cgttctccag ggagaagcct tgctgacaca
     9001 ggaacgcgag ctgattttcc agggtttcgt actgtttctc tgttgggcgg gtgccgagat
     9061 gcactttagc cccgtcgcga tgtgagagga gagcacagcg gtatgacttg gcgttgttcc
     9121 gcagaaagtc ttgccatgac tcgccttcca gggggcagga gtgggtatga tgcctgtcca
     9181 gcatctcgat tggcagggca tcgagcaggg cccgcttgtt cttcacgtgc cagtacaggg
     9241 taggctgctc aactcccagc ttttgagcga gtttccttgt cgtcaggcct tcgataccga
     9301 ctccattgag taattccaga gcagagttta tgactttgct cttgtccagt ctagacatgg
     9361 taattcgatg atccccctgg ggagagaggt cggtgattcg gtcaacgagg gagccgactg
     9421 ccgacgtgcg ctccggaggc ttgcagaatg cggaacaccg cgcgggcagg aacagggccc
     9481 acactaccgc cccacacccc gcctcccgca ccgccccttc ccggccgctg ctctcggcgc
     9541 gccctgctga gcagccgcta ttggccacag cccatcgcgg tcggcgcgct gccattgctc
     9601 cctggcgctg tccgtctgcg agggtactag tgagacgtgc ggcttccgtt tgtcacgtcc
     9661 ggcacgccgc gaaccgcaag gaaccttccc gacttagggg cggagcagga agcgtcgccg
     9721 gggggcccac aagggtagcg gcgaagatcc gggtgacgct gcgaacggac gtgaagaatg
     9781 tgcgagaccc agggtcggcg ccgctgcgtt tcccggaacc acgcccagag cagccgcgtc
     9841 cctgcgcaaa cccagggctg ccttggaaaa ggcgcaaccc caaccccgtg gaatt
//
