LOCUS       pUC19-U6-LwaCas13acrRNA-BsmBIcassette_(LTH151)        2240 bp    DNA     circular     04-MAY-2021
DEFINITION  .
ACCESSION   urn.local...i-dbuotxz
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     Serial Cloner Genbank Format SerialCloner_Type=DNA
            SerialCloner_Comments=[DEFINITION]: - [LOCUS]:
            pUC19-U6-+103gRNA.seq SerialCloner_Ends=0,0,,0,.
FEATURES             Location/Qualifiers
     misc_feature    13..330
                     /note="Geneious type: promoter"
                     /standard_name="U6 promoter"
     misc_RNA        331..366
                     /standard_name="LwaCas13a 5' direct repeat"
     misc_binding    367..387
     misc_feature    complement(368..373)
                     /note="Geneious type: misc_marker"
                     /standard_name="BsmBI"
     misc_feature    381..386
                     /note="Geneious type: misc_marker"
                     /standard_name="BsmBI"
     terminator      388..394
                     /standard_name="polIII terminator"
     rep_origin      complement(432..1105)
     CDS             complement(1250..2110)
                     /standard_name="ampR"
     promoter        complement(2111..2215)
                     /standard_name="Amp Promoter"
ORIGIN      
        1 gacgtcgcta gctgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc
       61 ggtaccaagg tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata
      121 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
      181 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
      241 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
      301 ttatatatct tgtggaaagg acgaaacacc gatttagact accccaaaaa cgaaggggac
      361 taaaacggag acgattaatg cgtctccttt ttttaagctt gggccgctcg aggtacctct
      421 ctacatatga catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      481 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      541 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
      601 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
      661 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
      721 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
      781 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
      841 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
      901 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
      961 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1021 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1081 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1141 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     1201 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     1261 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     1321 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     1381 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     1441 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     1501 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     1561 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     1621 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     1681 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     1741 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     1801 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     1861 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     1921 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     1981 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     2041 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     2101 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     2161 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     2221 ttccccgaaa agtgccacct
//
