LOCUS       Exported                5570 bp ds-DNA     circular SYN 09-MAY-2021
DEFINITION  pBBR1-KmR plasmid bearing J106 scRNA.
ACCESSION   .
VERSION     .
KEYWORDS    pPPC009.106
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, May 9, 2021 from SnapGene Viewer 5.2.3
            https://www.snapgene.com
COMMENT     Alias: pCK033.06
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     135..157
                     /label=M13/pUC Forward
                     /note="color: #75c6a9; direction: RIGHT"
     primer_bind     149..166
                     /label=M13 Forward
                     /note="color: #75c6a9; direction: RIGHT"
     primer_bind     150..166
                     /label=M13 fwd
                     /note="color: #75c6a9; direction: RIGHT"
     primer_bind     176..195
                     /label=T7
                     /note="color: #75c6a9; direction: RIGHT"
     promoter        176..194
                     /label=T7 promoter
                     /note="color: #346ee0"
     promoter        209..243
                     /label=J23119 (SpeI) promoter
                     /note="color: #ffef86; direction: RIGHT"
     misc_feature    238..243
                     /label=SpeI
                     /note="color: #c6c9d1"
     misc_feature    244..263
                     /label=J106
                     /note="color: #85dae9; direction: RIGHT"
     misc_feature    264..305
                     /label=JAD designed chimera RNA 3'UTR
                     /note="color: #84b0dc; direction: RIGHT"
     misc_feature    311..324
                     /label=Hairpin 3
                     /note="color: #b1ff67"
     misc_feature    325..347
                     /label=MS2 with 2bp stem extension
                     /note="color: #f58a5e"
     misc_feature    325..326
                     /label=Left stem extension
                     /note="color: #b4abac"
     misc_feature    346..347
                     /label=Right stem extension
                     /note="color: #b4abac"
     misc_feature    348..354
                     /label=SUP4 terminator
                     /note="color: #008080"
     terminator      355..722
                     /label=TrrnB
                     /note="color: #85dae9"
     terminator      648..715
                     /label=sglTerm
                     /note="color: #800040"
     misc_feature    723..728
                     /label=BspTI
                     /note="color: #f8d3a9"
     primer_bind     complement(747..767)
                     /label=T3
                     /note="color: #75c6a9; direction: LEFT"
     promoter        complement(747..765)
                     /label=T3 promoter
                     /note="color: #346ee0; direction: LEFT"
     primer_bind     complement(786..802)
                     /label=M13 Reverse
                     /note="color: #75c6a9; direction: LEFT"
     primer_bind     complement(786..802)
                     /label=M13 rev
                     /note="color: #75c6a9; direction: LEFT"
     primer_bind     complement(799..821)
                     /label=M13/pUC Reverse
                     /note="color: #75c6a9; direction: LEFT"
     protein_bind    810..826
                     /label=lac operator
                     /note="color: #ff9ccd"
     promoter        complement(834..864)
                     /label=lac promoter
                     /note="color: #346ee0; direction: LEFT"
     protein_bind    879..900
                     /label=CAP binding site
                     /note="color: #ff9ccd"
     CDS             complement(1305..2099)
                     /label=NeoR/KanR
                     /note="color: #ffef86; direction: LEFT"
     rep_origin      3579..4348
                     /label=pBBR1 oriV
                     /note="color: #75c6a9"
     CDS             4349..5011
                     /label=pBBR1 Rep
                     /note="color: #75c6a9; direction: RIGHT"
ORIGIN
        1 ttacaatttc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
       61 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
      121 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata
      181 cgactcacta tagggcgaat tggagctctt gacagctagc tcagtcctag gtataatact
      241 agtaggacgc ctttggtaac cgcgttttag agctagaaat agcaagttaa aataaggcta
      301 gtccgttatc aacttgaaaa agtggcacat gaggatcacc catgtgcttt ttttgaagct
      361 tgggcccgaa caaaaactca tctcagaaga ggatctgaat agcgccgtcg accatcatca
      421 tcatcatcat tgagtttaaa cggtctccag cttggctgtt ttggcggatg agagaagatt
      481 ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct
      541 ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt
      601 agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat
      661 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      721 ctcttaagct agaccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
      781 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      841 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
      901 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
      961 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgcatgca taaaaactgt
     1021 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
     1081 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat gggggtgggc
     1141 gaagaactcc agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac
     1201 gattccgaag cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag
     1261 gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca
     1321 agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg
     1381 aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg
     1441 tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca
     1501 ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg
     1561 tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct
     1621 tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg
     1681 cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc
     1741 attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc
     1801 tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc
     1861 acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc
     1921 agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct
     1981 gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg
     2041 aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg
     2101 cgaaacgatc ctcatcctgt ctcttgatca gatcttgatc ccctgcgcca tcagatcctt
     2161 ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc agagggcgcc
     2221 ccagctggca attccggttc gcttgctgtc cataaaaccg cccagtctag ctatcgccat
     2281 gtaagcccac tgcaagctac ctgctttctc tttgcgcttg cgttttccct tgtccagata
     2341 gcccagtagc tgacattcat cccaggtggc acttttcggg gaaatgtgcg cgcccgcgtt
     2401 cctgctggcg ctgggcctgt ttctggcgct ggacttcccg ctgttccgtc agcagctttt
     2461 cgcccacggc cttgatgatc gcggcggcct tggcctgcat atcccgattc aacggcccca
     2521 gggcgtccag aacgggcttc aggcgctccc gaaggtctcg ggccgtctct tgggcttgat
     2581 cggccttctt gcgcatctca cgcgctcctg cggcggcctg tagggcaggc tcatacccct
     2641 gccgaaccgc ttttgtcagc cggtcggcca cggcttccgg cgtctcaacg cgctttgaga
     2701 ttcccagctt ttcggccaat ccctgcggtg cataggcgcg tggctcgacc gcttgcgggc
     2761 tgatggtgac gtggcccact ggtggccgct ccagggcctc gtagaacgcc tgaatgcgcg
     2821 tgtgacgtgc cttgctgccc tcgatgcccc gttgcagccc tagatcggcc acagcggccg
     2881 caaacgtggt ctggtcgcgg gtcatctgcg ctttgttgcc gatgaactcc ttggccgaca
     2941 gcctgccgtc ctgcgtcagc ggcaccacga acgcggtcat gtgcgggctg gtttcgtcac
     3001 ggtggatgct ggccgtcacg atgcgatccg ccccgtactt gtccgccagc cacttgtgcg
     3061 ccttctcgaa gaacgccgcc tgctgttctt ggctggccga cttccaccat tccgggctgg
     3121 ccgtcatgac gtactcgacc gccaacacag cgtccttgcg ccgcttctct ggcagcaact
     3181 cgcgcagtcg gcccatcgct tcatcggtgc tgctggccgc ccagtgctcg ttctctggcg
     3241 tcctgctggc gtcagcgttg ggcgtctcgc gctcgcggta ggcgtgcttg agactggccg
     3301 ccacgttgcc cattttcgcc agcttcttgc atcgcatgat cgcgtatgcc gccatgcctg
     3361 cccctccctt ttggtgtcca accggctcga cgggggcagc gcaaggcggt gcctccggcg
     3421 ggccactcaa tgcttgagta tactcactag actttgcttc gcaaagtcgt gaccgcctac
     3481 ggcggctgcg gcgccctacg ggcttgctct ccgggcttcg ccctgcgcgg tcgctgcgct
     3541 cccttgccag cccgtggata tgtggacgat ggccgcgagc ggccaccggc tggctcgctt
     3601 cgctcggccc gtggacaacc ctgctggaca agctgatgga caggctgcgc ctgcccacga
     3661 gcttgaccac agggattgcc caccggctac ccagccttcg accacatacc caccggctcc
     3721 aactgcgcgg cctgcggcct tgccccatca atttttttaa ttttctctgg ggaaaagcct
     3781 ccggcctgcg gcctgcgcgc ttcgcttgcc ggttggacac caagtggaag gcgggtcaag
     3841 gctcgcgcag cgaccgcgca gcggcttggc cttgacgcgc ctggaacgac ccaagcctat
     3901 gcgagtgggg gcagtcgaag gcgaagcccg cccgcctgcc ccccgagcct cacggcggcg
     3961 agtgcggggg ttccaagggg gcagcgccac cttgggcaag gccgaaggcc gcgcagtcga
     4021 tcaacaagcc ccggaggggc cactttttgc cggaggggga gccgcgccga aggcgtgggg
     4081 gaaccccgca ggggtgccct tctttgggca ccaaagaact agatataggg cgaaatgcga
     4141 aagacttaaa aatcaacaac ttaaaaaagg ggggtacgca acagctcatt gcggcacccc
     4201 ccgcaatagc tcattgcgta ggttaaagaa aatctgtaat tgactgccac ttttacgcaa
     4261 cgcataattg ttgtcgcgct gccgaaaagt tgcagctgat tgcgcatggt gccgcaaccg
     4321 tgcggcaccc taccgcatgg agataagcat ggccacgcag tccagagaaa tcggcattca
     4381 agccaagaac aagcccggtc actgggtgca aacggaacgc aaagcgcatg aggcgtgggc
     4441 cgggcttatt gcgaggaaac ccacggcggc aatgctgctg catcacctcg tggcgcagat
     4501 gggccaccag aacgccgtgg tggtcagcca gaagacactt tccaagctca tcggacgttc
     4561 tttgcggacg gtccaatacg cagtcaagga cttggtggcc gagcgctgga tctccgtcgt
     4621 gaagctcaac ggccccggca ccgtgtcggc ctacgtggtc aatgaccgcg tggcgtgggg
     4681 ccagccccgc gaccagttgc gcctgtcggt gttcagtgcc gccgtggtgg ttgatcacga
     4741 cgaccaggac gaatcgctgt tggggcatgg cgacctgcgc cgcatcccga ccctgtatcc
     4801 gggcgagcag caactaccga ccggccccgg cgaggagccg cccagccagc ccggcattcc
     4861 gggcatggaa ccagacctgc cagccttgac cgaaacggag gaatgggaac ggcgcgggca
     4921 gcagcgcctg ccgatgcccg atgagccgtg ttttctggac gatggcgagc cgttggagcc
     4981 gccgacacgg gtcacgctgc cgcgccggta gcacttgggt tgcgcagcaa cccgtaagtg
     5041 cgctgttcca gactatcggc tgtagccgcc tcgccgccct ataccttgtc tgcctccccg
     5101 cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct
     5161 cgctaacgga ttcaccgttt ttatcaggct ctgggaggca gaataaatga tcatatcgtc
     5221 aattattacc tccacgggga gagcctgagc aaactggcct caggcatttg agaagcacac
     5281 ggtcacactg cttccggtag tcaataaacc ggtaaaccag caatagacat aagcggctat
     5341 ttaacgaccc tgccctgaac cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc
     5401 atccgcttat tatcacttat tcaggcgtag caaccaggcg tttaagggca ccaataactg
     5461 ccttaaaaaa attacgcccc gccctgccac tcatcgcagt acggcctatt ggttaaaaaa
     5521 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
//
