LOCUS       Exported                6639 bp ds-DNA     circular SYN 10-MAY-2021
DEFINITION  Alternative names: pCK058, pBBR1-KmR_J1-BBa_J23117-mRFP_J109.
ACCESSION   .
VERSION     .
KEYWORDS    pPPC017.109
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 10, 2021 from SnapGene Viewer 5.2.3
            https://www.snapgene.com
COMMENT     Alias: pCK058
FEATURES             Location/Qualifiers
     source          1..6639
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     primer_bind     135..157
                     /label=M13/pUC Forward
                     /note="color: #75c6a9; direction: RIGHT"
     primer_bind     149..166
                     /label=M13 Forward
                     /note="color: #75c6a9; direction: RIGHT"
     primer_bind     150..166
                     /label=M13 fwd
                     /note="color: #75c6a9; direction: RIGHT"
     primer_bind     176..195
                     /label=T7
                     /note="color: #75c6a9; direction: RIGHT"
     promoter        176..194
                     /label=T7 promoter
                     /note="color: #346ee0"
     misc_feature    189..203
                     /label=Flank
                     /note="color: #ffef86"
     promoter        209..243
                     /label=J23119 (SpeI) promoter
                     /note="color: #ffef86; direction: RIGHT"
     misc_feature    244..263
                     /label=J109
                     /note="color: #f58a5e; direction: RIGHT"
     misc_feature    264..305
                     /label=JAD designed chimera RNA 3'UTR
                     /note="color: #84b0dc; direction: RIGHT"
     misc_feature    311..324
                     /label=Hairpin 3
                     /note="color: #b1ff67"
     misc_feature    325..347
                     /label=MS2 with 2bp stem extension
                     /note="color: #f58a5e"
     misc_feature    325..326
                     /label=Left stem extension
                     /note="color: #b4abac"
     misc_feature    346..347
                     /label=Right stem extension
                     /note="color: #b4abac"
     misc_feature    348..354
                     /label=SUP4 terminator
                     /note="color: #008080"
     terminator      355..722
                     /label=TrrnB
                     /note="color: #85dae9"
     terminator      648..715
                     /label=sglTerm
                     /note="color: #800040"
     primer_bind     complement(747..767)
                     /label=T3
                     /note="color: #75c6a9; direction: LEFT"
     promoter        complement(747..765)
                     /label=T3 promoter
                     /note="color: #346ee0; direction: LEFT"
     primer_bind     complement(786..802)
                     /label=M13 rev
                     /note="color: #75c6a9; direction: LEFT"
     primer_bind     complement(786..802)
                     /label=M13 Reverse
                     /note="color: #75c6a9; direction: LEFT"
     primer_bind     complement(799..821)
                     /label=M13/pUC Reverse
                     /note="color: #75c6a9; direction: LEFT"
     protein_bind    810..826
                     /label=lac operator
                     /note="color: #ff9ccd"
     promoter        complement(834..864)
                     /label=lac promoter
                     /note="color: #346ee0; direction: LEFT"
     protein_bind    879..900
                     /label=CAP binding site
                     /note="color: #ff9ccd"
     misc_feature    complement(1017)
                     /label=AatII
                     /note="color: #d6b295; direction: LEFT"
     misc_feature    1018..1037
                     /label=J131
                     /note="color: #f58a5e"
     misc_feature    complement(1018..1037)
                     /label=J132*
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    1018..1027
                     /label=J133
                     /note="color: #f58a5e"
     misc_feature    1023..1192
                     /label=J1 upstream sequence
                     /note="color: #b7e6d7"
     misc_feature    complement(1027..1046)
                     /label=J130
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    1028..1047
                     /label=J129
                     /note="color: #f58a5e"
     misc_feature    complement(1037..1056)
                     /label=J128
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    1038..1057
                     /label=J127
                     /note="color: #f58a5e"
     misc_feature    complement(1047..1066)
                     /label=J126
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    1048..1067
                     /label=J125
                     /note="color: #f58a5e"
     misc_feature    complement(1057..1076)
                     /label=J124
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    1058..1077
                     /label=J123
                     /note="color: #f58a5e"
     misc_feature    complement(1067..1086)
                     /label=J122
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    1068..1087
                     /label=J121
                     /note="color: #f58a5e"
     misc_feature    complement(1077..1096)
                     /label=J120
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    1078..1097
                     /label=J119
                     /note="color: #f58a5e"
     misc_feature    complement(1087..1106)
                     /label=J118
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    1088..1107
                     /label=J117
                     /note="color: #f58a5e"
     misc_feature    complement(1097..1116)
                     /label=J116
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    1098..1117
                     /label=J115
                     /note="color: #f58a5e"
     misc_feature    complement(1107..1126)
                     /label=J114
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    1108..1127
                     /label=J113
                     /note="color: #f58a5e"
     misc_feature    complement(1117..1136)
                     /label=J112
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    1118..1137
                     /label=J111
                     /note="color: #f58a5e"
     misc_feature    complement(1127..1146)
                     /label=J110
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    1128..1147
                     /label=J109
                     /note="color: #b1ff67"
     misc_feature    complement(1137..1156)
                     /label=J108
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    1138..1157
                     /label=J107
                     /note="color: #b1ff67"
     misc_feature    complement(1147..1166)
                     /label=J106
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    1148..1167
                     /label=J105
                     /note="color: #f58a5e"
     misc_feature    complement(1157..1176)
                     /label=J104
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    1158..1177
                     /label=J103
                     /note="color: #f58a5e"
     misc_feature    complement(1167..1186)
                     /label=J102
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    1168..1187
                     /label=J101
                     /note="color: #f58a5e"
     promoter        1193..1227
                     /label=BBa_J23117
                     /note="color: #ffef86; direction: RIGHT"
     misc_feature    1228..1254
                     /label=Bujard RBS (strong)
                     /note="color: #db7aff"
     misc_feature    1255..1932
                     /label=mRFP
                     /note="color: #ff3737; direction: RIGHT"
     misc_feature    1951..2079
                     /label=dbl term
                     /note="color: #ff9ccd; direction: RIGHT"
     CDS             complement(2374..3168)
                     /label=NeoR/KanR
                     /note="color: #ffef86; direction: LEFT"
     rep_origin      4648..5417
                     /label=pBBR1 oriV
                     /note="color: #75c6a9"
     CDS             5418..6080
                     /label=pBBR1 Rep
                     /note="color: #75c6a9; direction: RIGHT"
ORIGIN
        1 ttacaatttc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
       61 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
      121 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata
      181 cgactcacta tagggcgaat tggagctctt gacagctagc tcagtcctag gtataatact
      241 agtaggtatc ctgcggtgtc ctggttttag agctagaaat agcaagttaa aataaggcta
      301 gtccgttatc aacttgaaaa agtggcacat gaggatcacc catgtgcttt ttttgaagct
      361 tgggcccgaa caaaaactca tctcagaaga ggatctgaat agcgccgtcg accatcatca
      421 tcatcatcat tgagtttaaa cggtctccag cttggctgtt ttggcggatg agagaagatt
      481 ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct
      541 ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt
      601 agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat
      661 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      721 ctcttaagct agaccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
      781 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      841 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
      901 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
      961 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgcatggc aattccgacg
     1021 tcgcctacgg tatccaccgg agacctatgg cagcctccgg ccgccatagg acacctttgg
     1081 ttgccaaggg tgacctatgg tgaccatggg ccaccacggg cgacctcagg tatcctgcgg
     1141 tgtcctgcgg ttaccaaagg cgtcctttgg gttccaccgg atacctccgg acttgacagc
     1201 tagctcagtc ctagggattg tgctagcgaa ttcattaaag aggagaaagg taccatggcg
     1261 agtagcgaag acgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt
     1321 aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag
     1381 accgctaaac tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc
     1441 ccgcagttcc agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac
     1501 ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt
     1561 ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt
     1621 aaactgcgtg gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt
     1681 tgggaagctt ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa
     1741 atgcgtctga aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg
     1801 gctaaaaaac cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc
     1861 tcccacaacg aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc
     1921 accggtgctt aaaaactcga gtaaggatct ccaggcatca aataaaacga aaggctcagt
     1981 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     2041 acactggctc accttcgggt gggcctttct gcgtttatat aaaaactgtt gtaattcatt
     2101 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
     2161 catcagcacc ttgtcgcctt gcgtataata tttgcccatg ggggtgggcg aagaactcca
     2221 gcatgagatc cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc
     2281 ccaacctttc atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg
     2341 cttggtcggt catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata
     2401 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc
     2461 ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg
     2521 gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat
     2581 gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg
     2641 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc
     2701 atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc
     2761 ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc
     2821 catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac
     2881 ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca
     2941 aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag
     3001 ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa
     3061 cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc
     3121 cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc
     3181 tcatcctgtc tcttgatcag atcttgatcc cctgcgccat cagatccttg gcggcaagaa
     3241 agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa
     3301 ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact
     3361 gcaagctacc tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct
     3421 gacattcatc ccaggtggca cttttcgggg aaatgtgcgc gcccgcgttc ctgctggcgc
     3481 tgggcctgtt tctggcgctg gacttcccgc tgttccgtca gcagcttttc gcccacggcc
     3541 ttgatgatcg cggcggcctt ggcctgcata tcccgattca acggccccag ggcgtccaga
     3601 acgggcttca ggcgctcccg aaggtctcgg gccgtctctt gggcttgatc ggccttcttg
     3661 cgcatctcac gcgctcctgc ggcggcctgt agggcaggct catacccctg ccgaaccgct
     3721 tttgtcagcc ggtcggccac ggcttccggc gtctcaacgc gctttgagat tcccagcttt
     3781 tcggccaatc cctgcggtgc ataggcgcgt ggctcgaccg cttgcgggct gatggtgacg
     3841 tggcccactg gtggccgctc cagggcctcg tagaacgcct gaatgcgcgt gtgacgtgcc
     3901 ttgctgccct cgatgccccg ttgcagccct agatcggcca cagcggccgc aaacgtggtc
     3961 tggtcgcggg tcatctgcgc tttgttgccg atgaactcct tggccgacag cctgccgtcc
     4021 tgcgtcagcg gcaccacgaa cgcggtcatg tgcgggctgg tttcgtcacg gtggatgctg
     4081 gccgtcacga tgcgatccgc cccgtacttg tccgccagcc acttgtgcgc cttctcgaag
     4141 aacgccgcct gctgttcttg gctggccgac ttccaccatt ccgggctggc cgtcatgacg
     4201 tactcgaccg ccaacacagc gtccttgcgc cgcttctctg gcagcaactc gcgcagtcgg
     4261 cccatcgctt catcggtgct gctggccgcc cagtgctcgt tctctggcgt cctgctggcg
     4321 tcagcgttgg gcgtctcgcg ctcgcggtag gcgtgcttga gactggccgc cacgttgccc
     4381 attttcgcca gcttcttgca tcgcatgatc gcgtatgccg ccatgcctgc ccctcccttt
     4441 tggtgtccaa ccggctcgac gggggcagcg caaggcggtg cctccggcgg gccactcaat
     4501 gcttgagtat actcactaga ctttgcttcg caaagtcgtg accgcctacg gcggctgcgg
     4561 cgccctacgg gcttgctctc cgggcttcgc cctgcgcggt cgctgcgctc ccttgccagc
     4621 ccgtggatat gtggacgatg gccgcgagcg gccaccggct ggctcgcttc gctcggcccg
     4681 tggacaaccc tgctggacaa gctgatggac aggctgcgcc tgcccacgag cttgaccaca
     4741 gggattgccc accggctacc cagccttcga ccacataccc accggctcca actgcgcggc
     4801 ctgcggcctt gccccatcaa tttttttaat tttctctggg gaaaagcctc cggcctgcgg
     4861 cctgcgcgct tcgcttgccg gttggacacc aagtggaagg cgggtcaagg ctcgcgcagc
     4921 gaccgcgcag cggcttggcc ttgacgcgcc tggaacgacc caagcctatg cgagtggggg
     4981 cagtcgaagg cgaagcccgc ccgcctgccc cccgagcctc acggcggcga gtgcgggggt
     5041 tccaaggggg cagcgccacc ttgggcaagg ccgaaggccg cgcagtcgat caacaagccc
     5101 cggaggggcc actttttgcc ggagggggag ccgcgccgaa ggcgtggggg aaccccgcag
     5161 gggtgccctt ctttgggcac caaagaacta gatatagggc gaaatgcgaa agacttaaaa
     5221 atcaacaact taaaaaaggg gggtacgcaa cagctcattg cggcaccccc cgcaatagct
     5281 cattgcgtag gttaaagaaa atctgtaatt gactgccact tttacgcaac gcataattgt
     5341 tgtcgcgctg ccgaaaagtt gcagctgatt gcgcatggtg ccgcaaccgt gcggcaccct
     5401 accgcatgga gataagcatg gccacgcagt ccagagaaat cggcattcaa gccaagaaca
     5461 agcccggtca ctgggtgcaa acggaacgca aagcgcatga ggcgtgggcc gggcttattg
     5521 cgaggaaacc cacggcggca atgctgctgc atcacctcgt ggcgcagatg ggccaccaga
     5581 acgccgtggt ggtcagccag aagacacttt ccaagctcat cggacgttct ttgcggacgg
     5641 tccaatacgc agtcaaggac ttggtggccg agcgctggat ctccgtcgtg aagctcaacg
     5701 gccccggcac cgtgtcggcc tacgtggtca atgaccgcgt ggcgtggggc cagccccgcg
     5761 accagttgcg cctgtcggtg ttcagtgccg ccgtggtggt tgatcacgac gaccaggacg
     5821 aatcgctgtt ggggcatggc gacctgcgcc gcatcccgac cctgtatccg ggcgagcagc
     5881 aactaccgac cggccccggc gaggagccgc ccagccagcc cggcattccg ggcatggaac
     5941 cagacctgcc agccttgacc gaaacggagg aatgggaacg gcgcgggcag cagcgcctgc
     6001 cgatgcccga tgagccgtgt tttctggacg atggcgagcc gttggagccg ccgacacggg
     6061 tcacgctgcc gcgccggtag cacttgggtt gcgcagcaac ccgtaagtgc gctgttccag
     6121 actatcggct gtagccgcct cgccgcccta taccttgtct gcctccccgc gttgcgtcgc
     6181 ggtgcatgga gccgggccac ctcgacctga atggaagccg gcggcacctc gctaacggat
     6241 tcaccgtttt tatcaggctc tgggaggcag aataaatgat catatcgtca attattacct
     6301 ccacggggag agcctgagca aactggcctc aggcatttga gaagcacacg gtcacactgc
     6361 ttccggtagt caataaaccg gtaaaccagc aatagacata agcggctatt taacgaccct
     6421 gccctgaacc gacgaccggg tcgaatttgc tttcgaattt ctgccattca tccgcttatt
     6481 atcacttatt caggcgtagc aaccaggcgt ttaagggcac caataactgc cttaaaaaaa
     6541 ttacgccccg ccctgccact catcgcagta cggcctattg gttaaaaaat gagctgattt
     6601 aacaaaaatt taacgcgaat tttaacaaaa tattaacgc
//
