LOCUS       Exported                6079 bp ds-DNA     circular SYN 19-OCT-2021
DEFINITION  Alternative names: pCK079, pRK2-GmR_J1-BBa_J23117-mRFP_AAV.
ACCESSION   .
VERSION     .
KEYWORDS    pPPC018.AAV
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 19, 2021 from SnapGene Viewer 4.3.11
            https://www.snapgene.com
COMMENT     Alias: pCK079
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     135..157
                     /label=M13/pUC Forward
     primer_bind     149..166
                     /label=M13 Forward
     primer_bind     150..166
                     /label=M13 fwd
     primer_bind     176..195
                     /label=T7
     promoter        176..194
                     /label=T7 promoter
     misc_feature    203..207
                     /label=MCS
     promoter        227..261
                     /label=J23119 (SpeI) promoter
     misc_feature    262..281
                     /label=hAAVS1
     misc_feature    282..323
                     /label=JAD designed chimera RNA 3'UTR
     misc_feature    328..342
                     /label=Streptococcus pyogenes tracrRNAterminator
     misc_feature    329..342
                     /label=Hairpin 3
     misc_feature    343..365
                     /label=MS2 with 2bp stem extension
     misc_feature    343..344
                     /label=Left stem extension
     misc_feature    364..365
                     /label=Right stem extension
     misc_feature    366..372
                     /label=SUP4 terminator
     terminator      373..740
                     /label=TrrnB
     misc_feature    504..521
                     /label=pBad_r
     terminator      666..733
                     /label=sglTerm
     primer_bind     complement(765..785)
                     /label=T3
     promoter        complement(765..783)
                     /label=T3 promoter
     primer_bind     complement(804..820)
                     /label=M13 rev
     primer_bind     complement(804..820)
                     /label=M13 Reverse
     primer_bind     complement(817..839)
                     /label=M13/pUC Reverse
     protein_bind    828..844
                     /label=lac operator
     promoter        complement(852..882)
                     /label=lac promoter
     protein_bind    897..918
                     /label=CAP binding site
     misc_feature    complement(1035)
                     /label=AatII
     misc_feature    1036..1055
                     /label=J131
     misc_feature    complement(1036..1055)
                     /label=J132*
     misc_feature    1036..1045
                     /label=J133
     misc_feature    1041..1210
                     /label=J1 upstream sequence
     misc_feature    complement(1045..1064)
                     /label=J130
     misc_feature    1046..1065
                     /label=J129
     misc_feature    complement(1055..1074)
                     /label=J128
     misc_feature    1056..1075
                     /label=J127
     misc_feature    complement(1065..1084)
                     /label=J126
     misc_feature    1066..1085
                     /label=J125
     misc_feature    complement(1075..1094)
                     /label=J124
     misc_feature    1076..1095
                     /label=J123
     misc_feature    complement(1085..1104)
                     /label=J122
     misc_feature    1086..1105
                     /label=J121
     misc_feature    complement(1095..1114)
                     /label=J120
     misc_feature    1096..1115
                     /label=J119
     misc_feature    complement(1105..1124)
                     /label=J118
     misc_feature    1106..1125
                     /label=J117
     misc_feature    complement(1115..1134)
                     /label=J116
     misc_feature    1116..1135
                     /label=J115
     misc_feature    complement(1125..1144)
                     /label=J114
     misc_feature    1126..1145
                     /label=J113
     misc_feature    complement(1135..1154)
                     /label=J112
     misc_feature    1136..1155
                     /label=J111
     misc_feature    complement(1145..1164)
                     /label=J110
     misc_feature    1146..1165
                     /label=J109
     misc_feature    complement(1155..1174)
                     /label=J108
     misc_feature    1156..1175
                     /label=J107
     misc_feature    complement(1165..1184)
                     /label=J106
     misc_feature    1166..1185
                     /label=J105
     misc_feature    complement(1175..1194)
                     /label=J104
     misc_feature    1176..1195
                     /label=J103
     misc_feature    complement(1185..1204)
                     /label=J102
     misc_feature    1186..1205
                     /label=J101
     promoter        1211..1245
                     /label=BBa_J23117
     misc_feature    1246..1272
                     /label=Bujard RBS (strong)
     misc_feature    1273..1950
                     /label=mRFP
     misc_feature    1969..2097
                     /label=dbl term
     misc_feature    1975..2030
                     /label=Effective terminator
     primer_bind     complement(2133..2152)
                     /label=oCK089
     terminator      complement(2220..2309)
                     /label=ECK120029600
     misc_feature    complement(2220..2309)
                     /label=SpyTerminator
     primer_bind     complement(2358..2377)
                     /label=oCK076
     gene            complement(2408..3556)
                     /label=trfA
     promoter        complement(3672..3712)
                     /label=NEOKAN_promoter
     rep_origin      complement(3949..4480)
                     /direction=LEFT
                     /label=oriV
     terminator      complement(4592..4686)
                     /label=lambda t0 terminator
     CDS             complement(4958..5491)
                     /label=gentamicin-3-acetyltransferase
     promoter        complement(5680..5708)
                     /label=Pc promoter
     misc_feature    complement(5680..5688)
                     /label=-10 box
     misc_feature    complement(5703..5708)
                     /label=-35 box
     primer_bind     5813..5832
                     /label=oCK089
     promoter        complement(5833..5873)
                     /label=NEOKAN_promoter
     misc_feature    5874..5879
                     /label=AatII
ORIGIN
        1 ttacaatttc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
       61 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
      121 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata
      181 cgactcacta tagggcgaat tggagctcca ccgcggtggc ggccgcttga cagctagctc
      241 agtcctaggt ataatactag tggggccact agggacagga tgttttagag ctagaaatag
      301 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcacatga ggatcaccca
      361 tgtgcttttt ttgaagcttg ggcccgaaca aaaactcatc tcagaagagg atctgaatag
      421 cgccgtcgac catcatcatc atcatcattg agtttaaacg gtctccagct tggctgtttt
      481 ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg
      541 ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac
      601 tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg
      661 aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      721 ctgttgtttg tcggtgaact gggcccggta cccagctttt gttcccttta gtgagggtta
      781 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
      841 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
      901 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
      961 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     1021 cgcatggcaa ttccgacgtc gcctacggta tccaccggag acctatggca gcctccggcc
     1081 gccataggac acctttggtt gccaagggtg acctatggtg accatgggcc accacgggcg
     1141 acctcaggta tcctgcggtg tcctgcggtt accaaaggcg tcctttgggt tccaccggat
     1201 acctccggac ttgacagcta gctcagtcct agggattgtg ctagcgaatt cattaaagag
     1261 gagaaaggta ccatggcgag tagcgaagac gttatcaaag agttcatgcg tttcaaagtt
     1321 cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt
     1381 ccgtacgaag gtacccagac cgctaaactg aaagttacca aaggtggtcc gctgccgttc
     1441 gcttgggaca tcctgtcccc gcagttccag tacggttcca aagcttacgt taaacacccg
     1501 gctgacatcc cggactacct gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt
     1561 atgaacttcg aagacggtgg tgttgttacc gttacccagg actcctccct gcaagacggt
     1621 gagttcatct acaaagttaa actgcgtggt accaacttcc cgtccgacgg tccggttatg
     1681 cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta tgtacccgga agacggtgct
     1741 ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa
     1801 gttaaaacca cctacatggc taaaaaaccg gttcagctgc cgggtgctta caaaaccgac
     1861 atcaaactgg acatcacctc ccacaacgaa gactacacca tcgttgaaca gtacgaacgt
     1921 gctgaaggtc gtcactccac cggtgcttaa aaactcgagt aaggatctcc aggcatcaaa
     1981 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     2041 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatataa
     2101 aaactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
     2161 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcgaattct
     2221 tcagccaaaa aacttaagac cgccggtctt gtccactacc ttgcagtaat gcggtggaca
     2281 ggatcggcgg ttttcttttc tcttctcaac ctaggctaca gccgatagtc tggaacagcg
     2341 cacttacggg acctagggcg atgcaggtgg ctgctgaacc cccagccgga actgacccca
     2401 caaggcccta gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt tccaccaggc
     2461 cgctgcctcg caactcttcg caggcttcgc cgacctgctc gcgccacttc ttcacgcggg
     2521 tggaatccga tccgcacatg aggcggaagg tttccagctt gagcgggtac ggctcccggt
     2581 gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg tacttctccc
     2641 atatgaattt cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg tcgatcagga
     2701 cctggcaacg ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca
     2761 ccgattccag gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg
     2821 acaggcattc ctcggccttc gtgtaatacc ggccattgat cgaccagccc aggtcctggc
     2881 aaagctcgta gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca
     2941 acacctgctg ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga
     3001 tcttcacgtc cttgttgacg tggaaaatga ccttgttttg cagcgcctcg cgcgggattt
     3061 tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc gtttggcatc gctcgcatcg
     3121 tgtccggcca cggcgcaata tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg
     3181 tgtgtttcag caacgcggcc tgcttggctt cgctgacctg ttttgccagg tcctcgccgg
     3241 cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca
     3301 aacctgccgc ctcctgttcg agacgacgcg aacgctccac ggcggccgat ggcgcgggca
     3361 gggcaggggg agccagttgc acgctgtcgc gctcgatctt ggccgtagct tgctggacta
     3421 tcgagccgac ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg
     3481 tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat gccttccggt
     3541 caaacgtccg attcattcac cctccttgcg ggattgcccc ggaattaatt ccccggatcg
     3601 atccgtcgat cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt
     3661 tactttgcag ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct
     3721 tgctgtccat aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg
     3781 ctttctcttt gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccg
     3841 gggtcagcac cgtttctgcg gactggcttt ctacgtggct gccatttttg gggtgaggcc
     3901 gttcgcggcc gaggggcgca gcccctgggg ggatgggagg cccgcgttag cgggccggga
     3961 gggttcgaga agggggggca ccccccttcg gcgtgcgcgg tcacgcgcac agggcgcagc
     4021 cctggttaaa aacaaggttt ataaatattg gtttaaaagc aggttaaaag acaggttagc
     4081 ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga ttttctgcct gtggacagcc
     4141 cctcaaatgt caataggtgc gcccctcatc tgtcagcact ctgcccctca agtgtcaagg
     4201 atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg tcaataccgc agggcactta
     4261 tccccaggct tgtccacatc atctgtggga aactcgcgta aaatcaggcg ttttcgccga
     4321 tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg agcctgcccc tcatctgtca
     4381 acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc cgcccctcat ctgtcagtga
     4441 gggccaagtt ttccgcgagg tatccacaac gccggcggcc ctacatggct ctgctgtagt
     4501 gagtgggttg cgctccggca gcggtcctga tcccccgcag aaaaaaagga tctcaagaag
     4561 atcctttgat cttttctaca ctagtgcttg gattctcacc aataaaaaac gcccggcggc
     4621 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
     4681 ggagtccaag cgagctcacc ttcgggagcg cctgaagccc gttctggacg ccctggggcc
     4741 gttgaatcgg gatatgcagg ccaaggccgc cgcgatcatc aaggccgtgg gcgaaaagct
     4801 gctgacggaa cagcgggaag tccagcgcca gaaacaggcc cagcgccagc aggaacgcgg
     4861 gcgcgcacat ttccccgaaa agtgccacct ggcggcgttg tgacaattta ccgaacaact
     4921 ccgcggccgg gaagccgatc tcggcttgaa cgaattgtta ggtggcggta cttgggtcga
     4981 tatcaaagtg catcacttct tcccgtatgc ccaactttgt atagagagcc actgcgggat
     5041 cgtcaccgta atctgcttgc acgtagatca cataagcacc aagcgcgttg gcctcatgct
     5101 tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg gtgctcgccg gagactgcga
     5161 gatcatagat atagatctca ctacgcggct gctcaaacct gggcagaacg taagccgcga
     5221 gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc gatgaatgtc ttactacgga
     5281 gcaagttccc gaggtaatcg gagtccggct gatgttggga gtaggtggct acgtctccga
     5341 actcacgacc gaaaagatca agagcagccc gcatggattt gacttggtca gggccgagcc
     5401 tacatgtgcg aatgatgccc atacttgagc cacctaactt tgttttaggg cgactgccct
     5461 gctgcgtaac atcgttgctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga
     5521 cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac aaaaaaacag
     5581 tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt
     5641 ctggaccagt tgcgtgagcg catacgctac ttgcattaca gtttacgaac cgaacaggct
     5701 tatgtcaact gggttcgtgc cttcatccgt ttccacggtg tgcgtccatg ggcaaatatt
     5761 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg
     5821 atggcttcca tggcttccca accttaccag agggcgcccc agctggcaat tccgacgtcg
     5881 tcgaatttgc tttcgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc
     5941 aaccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact
     6001 catcgcagta cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6061 tttaacaaaa tattaacgc
//
