LOCUS       Exported                6261 bp ds-DNA     circular SYN 10-MAY-2021
DEFINITION  Alternative names: pCK120.306, pBBR1-GmR_J3-BBa_J23117-mRFP_J306.
ACCESSION   .
VERSION     .
KEYWORDS    pPPC020.306
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 10, 2021 from SnapGene Viewer 5.2.3
            https://www.snapgene.com
COMMENT     Alias: pCK120.306
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     135..157
                     /label=M13/pUC Forward
                     /note="color: #75c6a9; direction: RIGHT"
     primer_bind     149..166
                     /label=M13 Forward
                     /note="color: #75c6a9; direction: RIGHT"
     primer_bind     150..166
                     /label=M13 fwd
                     /note="color: #75c6a9; direction: RIGHT"
     primer_bind     176..195
                     /label=T7
                     /note="color: #75c6a9; direction: RIGHT"
     promoter        176..194
                     /label=T7 promoter
                     /note="color: #346ee0"
     promoter        209..243
                     /label=J23119 (SpeI) promoter
                     /note="color: #ffef86; direction: RIGHT"
     misc_feature    244..263
                     /label=J306
                     /note="color: #75c6a9; direction: RIGHT"
     misc_feature    264..305
                     /label=JAD designed chimera RNA 3'UTR
                     /note="color: #84b0dc; direction: RIGHT"
     misc_feature    311..324
                     /label=Hairpin 3
                     /note="color: #b1ff67"
     misc_feature    325..347
                     /label=MS2 with 2bp stem extension
                     /note="color: #f58a5e"
     misc_feature    325..326
                     /label=Left stem extension
                     /note="color: #b4abac"
     misc_feature    346..347
                     /label=Right stem extension
                     /note="color: #b4abac"
     misc_feature    348..354
                     /label=SUP4 terminator
                     /note="color: #008080"
     terminator      355..722
                     /label=TrrnB
                     /note="color: #85dae9"
     terminator      648..715
                     /label=sglTerm
                     /note="color: #800040"
     CDS             complement(734..790)
                     /label=lacZ-alpha
                     /note="color: #ffef86; direction: LEFT"
     primer_bind     complement(747..767)
                     /label=T3
                     /note="color: #75c6a9; direction: LEFT"
     promoter        complement(747..765)
                     /label=T3 promoter
                     /note="color: #346ee0; direction: LEFT"
     primer_bind     complement(786..802)
                     /label=M13 rev
                     /note="color: #75c6a9; direction: LEFT"
     primer_bind     complement(786..802)
                     /label=M13 Reverse
                     /note="color: #75c6a9; direction: LEFT"
     primer_bind     complement(799..821)
                     /label=M13/pUC Reverse
                     /note="color: #75c6a9; direction: LEFT"
     protein_bind    810..826
                     /label=lac operator
                     /note="color: #ff9ccd"
     promoter        complement(834..864)
                     /label=lac promoter
                     /note="color: #346ee0; direction: LEFT"
     protein_bind    879..900
                     /label=CAP binding site
                     /note="color: #ff9ccd"
     misc_feature    1023..1192
                     /label=J3
                     /note="color: #85dae9"
     misc_feature    1128..1147
                     /label=J309
                     /note="color: #b7e6d7"
     misc_feature    complement(1137..1156)
                     /label=J308
                     /note="color: #75c6a9; direction: LEFT"
     misc_feature    1138..1157
                     /label=J307
                     /note="color: #b7e6d7"
     misc_feature    complement(1147..1166)
                     /label=J306
                     /note="color: #75c6a9; direction: LEFT"
     promoter        1193..1227
                     /label=BBa_J23117
                     /note="color: #ffef86; direction: RIGHT"
     misc_feature    1228..1254
                     /label=Bujard RBS (strong)
                     /note="color: #db7aff"
     misc_feature    1258..1932
                     /label=mRFP
                     /note="color: #ff3737; direction: RIGHT"
     misc_feature    1951..2079
                     /label=dbl term
                     /note="color: #ff9ccd; direction: RIGHT"
     CDS             2456..2989
                     /label=gentamicin-3-acetyltransferase
                     /note="color: #84b0dc; direction: RIGHT"
     rep_origin      4272..5041
                     /label=pBBR1 oriV
                     /note="color: #75c6a9"
     CDS             5042..5704
                     /label=pBBR1 Rep
                     /note="color: #75c6a9; direction: RIGHT"
ORIGIN
        1 ttacaatttc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
       61 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
      121 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata
      181 cgactcacta tagggcgaat tggagctctt gacagctagc tcagtcctag gtataatact
      241 agtttgtgtc cagaacgctc cgtgttttag agctagaaat agcaagttaa aataaggcta
      301 gtccgttatc aacttgaaaa agtggcacat gaggatcacc catgtgcttt ttttgaagct
      361 tgggcccgaa caaaaactca tctcagaaga ggatctgaat agcgccgtcg accatcatca
      421 tcatcatcat tgagtttaaa cggtctccag cttggctgtt ttggcggatg agagaagatt
      481 ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct
      541 ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt
      601 agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat
      661 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      721 ctcttaagct agaccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
      781 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      841 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
      901 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
      961 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgcatggc aattccgacg
     1021 tcagcatttg cgatcattca cgcagcgctt attcagttgc tcactgcgat gtcataatca
     1081 tcgctacgag ctgtgaaaga tgcataaagc tcgtacgacg cgttcgctcg tctcctcact
     1141 tctcctacgg agcgttctgg acacaacgtc gtcttgaagt tgcgattata gattgacagc
     1201 tagctcagtc ctagggattg tgctagcgaa ttcattaaag aggagaaagg taccatggcg
     1261 agtagcgaag acgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt
     1321 aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag
     1381 accgctaaac tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc
     1441 ccgcagttcc agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac
     1501 ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt
     1561 ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt
     1621 aaactgcgtg gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt
     1681 tgggaagctt ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa
     1741 atgcgtctga aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg
     1801 gctaaaaaac cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc
     1861 tcccacaacg aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc
     1921 accggtgctt aaaaactcga gtaaggatct ccaggcatca aataaaacga aaggctcagt
     1981 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     2041 acactggctc accttcgggt gggcctttct gcgtttatat aaaaactgtt gtaattcatt
     2101 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
     2161 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gacgcacacc gtggaaacgg
     2221 atgaaggcac gaacccagtt gacataagcc tgttcggttc gtaaactgta atgcaagtag
     2281 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     2341 gtggcggttt tcatggcttg ttatgactgt ttttttgtac agtctatgcc tcgggcatcc
     2401 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     2461 acgcagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taggtggctc
     2521 aagtatgggc atcattcgca catgtaggct cggccctgac caagtcaaat ccatgcgggc
     2581 tgctcttgat cttttcggtc gtgagttcgg agacgtagcc acctactccc aacatcagcc
     2641 ggactccgat tacctcggga acttgctccg tagtaagaca ttcatcgcgc ttgctgcctt
     2701 cgaccaagaa gcggttgttg gcgctctcgc ggcttacgtt ctgcccaggt ttgagcagcc
     2761 gcgtagtgag atctatatct atgatctcgc agtctccggc gagcaccgga ggcagggcat
     2821 tgccaccgcg ctcatcaatc tcctcaagca tgaggccaac gcgcttggtg cttatgtgat
     2881 ctacgtgcaa gcagattacg gtgacgatcc cgcagtggct ctctatacaa agttgggcat
     2941 acgggaagaa gtgatgcact ttgatatcga cccaagtacc gccacctaac aattcgttca
     3001 agccgagatc ggcttcccgg ccgcggagtt gttcggtaaa ttgtcacaac gccgccaggt
     3061 ggcacttttc ggggaaatgt gcgcgcccgc gttcctgctg gcgctgggcc tgtttctggc
     3121 gctggacttc ccgctgttcc gtcagcagct tttcgcccac ggccttgatg atcgcggcgg
     3181 ccttggcctg catatcccga ttcaacggcc ccagggcgtc cagaacgggc ttcaggcgct
     3241 cccgaaggtc tcgggccgtc tcttgggctt gatcggcctt cttgcgcatc tcacgcgctc
     3301 ctgcggcggc ctgtagggca ggctcatacc cctgccgaac cgcttttgtc agccggtcgg
     3361 ccacggcttc cggcgtctca acgcgctttg agattcccag cttttcggcc aatccctgcg
     3421 gtgcataggc gcgtggctcg accgcttgcg ggctgatggt gacgtggccc actggtggcc
     3481 gctccagggc ctcgtagaac gcctgaatgc gcgtgtgacg tgccttgctg ccctcgatgc
     3541 cccgttgcag ccctagatcg gccacagcgg ccgcaaacgt ggtctggtcg cgggtcatct
     3601 gcgctttgtt gccgatgaac tccttggccg acagcctgcc gtcctgcgtc agcggcacca
     3661 cgaacgcggt catgtgcggg ctggtttcgt cacggtggat gctggccgtc acgatgcgat
     3721 ccgccccgta cttgtccgcc agccacttgt gcgccttctc gaagaacgcc gcctgctgtt
     3781 cttggctggc cgacttccac cattccgggc tggccgtcat gacgtactcg accgccaaca
     3841 cagcgtcctt gcgccgcttc tctggcagca actcgcgcag tcggcccatc gcttcatcgg
     3901 tgctgctggc cgcccagtgc tcgttctctg gcgtcctgct ggcgtcagcg ttgggcgtct
     3961 cgcgctcgcg gtaggcgtgc ttgagactgg ccgccacgtt gcccattttc gccagcttct
     4021 tgcatcgcat gatcgcgtat gccgccatgc ctgcccctcc cttttggtgt ccaaccggct
     4081 cgacgggggc agcgcaaggc ggtgcctccg gcgggccact caatgcttga gtatactcac
     4141 tagactttgc ttcgcaaagt cgtgaccgcc tacggcggct gcggcgccct acgggcttgc
     4201 tctccgggct tcgccctgcg cggtcgctgc gctcccttgc cagcccgtgg atatgtggac
     4261 gatggccgcg agcggccacc ggctggctcg cttcgctcgg cccgtggaca accctgctgg
     4321 acaagctgat ggacaggctg cgcctgccca cgagcttgac cacagggatt gcccaccggc
     4381 tacccagcct tcgaccacat acccaccggc tccaactgcg cggcctgcgg ccttgcccca
     4441 tcaatttttt taattttctc tggggaaaag cctccggcct gcggcctgcg cgcttcgctt
     4501 gccggttgga caccaagtgg aaggcgggtc aaggctcgcg cagcgaccgc gcagcggctt
     4561 ggccttgacg cgcctggaac gacccaagcc tatgcgagtg ggggcagtcg aaggcgaagc
     4621 ccgcccgcct gccccccgag cctcacggcg gcgagtgcgg gggttccaag ggggcagcgc
     4681 caccttgggc aaggccgaag gccgcgcagt cgatcaacaa gccccggagg ggccactttt
     4741 tgccggaggg ggagccgcgc cgaaggcgtg ggggaacccc gcaggggtgc ccttctttgg
     4801 gcaccaaaga actagatata gggcgaaatg cgaaagactt aaaaatcaac aacttaaaaa
     4861 aggggggtac gcaacagctc attgcggcac cccccgcaat agctcattgc gtaggttaaa
     4921 gaaaatctgt aattgactgc cacttttacg caacgcataa ttgttgtcgc gctgccgaaa
     4981 agttgcagct gattgcgcat ggtgccgcaa ccgtgcggca ccctaccgca tggagataag
     5041 catggccacg cagtccagag aaatcggcat tcaagccaag aacaagcccg gtcactgggt
     5101 gcaaacggaa cgcaaagcgc atgaggcgtg ggccgggctt attgcgagga aacccacggc
     5161 ggcaatgctg ctgcatcacc tcgtggcgca gatgggccac cagaacgccg tggtggtcag
     5221 ccagaagaca ctttccaagc tcatcggacg ttctttgcgg acggtccaat acgcagtcaa
     5281 ggacttggtg gccgagcgct ggatctccgt cgtgaagctc aacggccccg gcaccgtgtc
     5341 ggcctacgtg gtcaatgacc gcgtggcgtg gggccagccc cgcgaccagt tgcgcctgtc
     5401 ggtgttcagt gccgccgtgg tggttgatca cgacgaccag gacgaatcgc tgttggggca
     5461 tggcgacctg cgccgcatcc cgaccctgta tccgggcgag cagcaactac cgaccggccc
     5521 cggcgaggag ccgcccagcc agcccggcat tccgggcatg gaaccagacc tgccagcctt
     5581 gaccgaaacg gaggaatggg aacggcgcgg gcagcagcgc ctgccgatgc ccgatgagcc
     5641 gtgttttctg gacgatggcg agccgttgga gccgccgaca cgggtcacgc tgccgcgccg
     5701 gtagcacttg ggttgcgcag caacccgtaa gtgcgctgtt ccagactatc ggctgtagcc
     5761 gcctcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
     5821 ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcaccg tttttatcag
     5881 gctctgggag gcagaataaa tgatcatatc gtcaattatt acctccacgg ggagagcctg
     5941 agcaaactgg cctcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa
     6001 accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg aaccgacgac
     6061 cgggtcgaat ttgctttcga atttctgcca ttcatccgct tattatcact tattcaggcg
     6121 tagcaccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc
     6181 actcatcgca gtcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     6241 attttaacaa aatattaacg c
//
