LOCUS       Exported                8324 bp ds-DNA     circular SYN 10-MAY-2021
DEFINITION  Alternative names: pCK134, 
            pBBR1-GmR_J3-BBa_J23117-GTPCH_J3-BBa_J23117-PTPS_J3-BBa_J23117-SR.
ACCESSION   .
VERSION     .
KEYWORDS    pPPC027.306
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 10, 2021 from SnapGene Viewer 5.2.3
            https://www.snapgene.com
COMMENT     Alias: pCK134
FEATURES             Location/Qualifiers
     source          1..8324
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        209..243
                     /label=J23119 (SpeI) promoter
                     /note="color: #ffef86; direction: RIGHT"
     misc_feature    238..243
                     /label=SpeI
                     /note="color: #c6c9d1"
     misc_feature    244..263
                     /label=J306
                     /note="color: #75c6a9; direction: RIGHT"
     misc_feature    264..305
                     /label=JAD designed chimera RNA 3'UTR
                     /note="color: #84b0dc; direction: RIGHT"
     misc_feature    311..324
                     /label=Hairpin 3
                     /note="color: #b1ff67"
     misc_feature    325..347
                     /label=MS2 with 2bp stem extension
                     /note="color: #f58a5e"
     misc_feature    348..354
                     /label=SUP4 terminator
                     /note="color: #008080"
     terminator      355..722
                     /label=TrrnB
                     /note="color: #85dae9"
     misc_feature    723..728
                     /label=BspTI
                     /note="color: #f8d3a9"
     misc_feature    1017..1022
                     /label=EcoRV
                     /note="color: #ffef86"
     misc_feature    1023..1192
                     /label=J3
                     /note="color: #85dae9"
     misc_feature    1128..1147
                     /label=J309
                     /note="color: #b7e6d7"
     misc_feature    complement(1137..1156)
                     /label=J308
                     /note="color: #75c6a9; direction: LEFT"
     misc_feature    1138..1157
                     /label=J307
                     /note="color: #b7e6d7"
     misc_feature    complement(1147..1166)
                     /label=J306
                     /note="color: #75c6a9; direction: LEFT"
     promoter        1193..1227
                     /label=BBa_J23117
                     /note="color: #ffef86; direction: RIGHT"
     misc_feature    1228..1254
                     /label=Bujard RBS (strong)
                     /note="color: #db7aff"
     misc_feature    1255..1941
                     /label=EcGTPCH_His-tagged
                     /note="color: #84b0dc; direction: RIGHT"
     misc_feature    1942..2070
                     /label=dbl term
                     /note="color: #ff9ccd; direction: RIGHT"
     misc_feature    2074..2243
                     /label=J3
                     /note="color: #85dae9"
     misc_feature    2179..2198
                     /label=J309
                     /note="color: #b7e6d7"
     misc_feature    complement(2188..2207)
                     /label=J308
                     /note="color: #75c6a9; direction: LEFT"
     misc_feature    2189..2208
                     /label=J307
                     /note="color: #b7e6d7"
     misc_feature    complement(2198..2217)
                     /label=J306
                     /note="color: #75c6a9; direction: LEFT"
     promoter        2244..2278
                     /label=BBa_J23117
                     /note="color: #ffef86; direction: RIGHT"
     misc_feature    2279..2305
                     /label=Bujard RBS (strong)
                     /note="color: #db7aff"
     misc_feature    2306..2764
                     /label=MaPTPS - Ec-optimized
                     /note="color: #75c6a9; direction: RIGHT"
     misc_feature    2765..2893
                     /label=dbl term
                     /note="color: #ff9ccd; direction: RIGHT"
     misc_feature    2897..3066
                     /label=J3
                     /note="color: #85dae9"
     misc_feature    3002..3021
                     /label=J309
                     /note="color: #b7e6d7"
     misc_feature    complement(3011..3030)
                     /label=J308
                     /note="color: #75c6a9; direction: LEFT"
     misc_feature    3012..3031
                     /label=J307
                     /note="color: #b7e6d7"
     misc_feature    complement(3021..3040)
                     /label=J306
                     /note="color: #75c6a9; direction: LEFT"
     promoter        3067..3101
                     /label=BBa_J23117
                     /note="color: #ffef86; direction: RIGHT"
     misc_feature    3102..3128
                     /label=Bujard RBS (strong)
                     /note="color: #db7aff"
     misc_feature    3129..3995
                     /label=MaSR - Ec-optimized
                     /note="color: #f58a5e; direction: RIGHT"
     misc_feature    3999..4004
                     /label=XhoI
                     /note="color: #84b0dc"
     misc_feature    4014..4142
                     /label=dbl term
                     /note="color: #ff9ccd; direction: RIGHT"
     CDS             4519..5052
                     /label=gentamicin-3-acetyltransferase
                     /note="color: #84b0dc; direction: RIGHT"
     rep_origin      6335..7104
                     /label=pBBR1 oriV
                     /note="color: #75c6a9"
     CDS             7105..7767
                     /label=pBBR1 Rep
                     /note="color: #75c6a9; direction: RIGHT"
ORIGIN
        1 ttacaatttc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
       61 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
      121 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata
      181 cgactcacta tagggcgaat tggagctctt gacagctagc tcagtcctag gtataatact
      241 agtttgtgtc cagaacgctc cgtgttttag agctagaaat agcaagttaa aataaggcta
      301 gtccgttatc aacttgaaaa agtggcacat gaggatcacc catgtgcttt ttttgaagct
      361 tgggcccgaa caaaaactca tctcagaaga ggatctgaat agcgccgtcg accatcatca
      421 tcatcatcat tgagtttaaa cggtctccag cttggctgtt ttggcggatg agagaagatt
      481 ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct
      541 ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt
      601 agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat
      661 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      721 ctcttaagct agaccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
      781 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      841 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
      901 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
      961 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgcatggc aattccgata
     1021 tcagcatttg cgatcattca cgcagcgctt attcagttgc tcactgcgat gtcataatca
     1081 tcgctacgag ctgtgaaaga tgcataaagc tcgtacgacg cgttcgctcg tctcctcact
     1141 tctcctacgg agcgttctgg acacaacgtc gtcttgaagt tgcgattata gattgacagc
     1201 tagctcagtc ctagggattg tgctagcgaa ttcattaaag aggagaaagg taccatgcat
     1261 caccatcacc atcacccatc actcagtaaa gaagcggccc tggttcatga agcgttagtt
     1321 gcgcgaggac tggaaacacc gctgcgcccg cccgtgcatg aaatggataa cgaaacgcgc
     1381 aaaagcctta ttgctggtca tatgaccgaa atcatgcagc tgctgaatct cgacctggct
     1441 gatgacagtt tgatggaaac gccgcatcgc atcgctaaaa tgtatgtcga tgaaattttc
     1501 tccggtctgg attacgccaa tttcccgaaa atcaccctca ttgaaaacaa aatgaaggtc
     1561 gatgaaatgg tcaccgtgcg cgatatcact ctgaccagca cctgtgaaca ccattttgtt
     1621 accatcgatg gcaaagcgac ggtggcctat atcccgaaag attcggtgat cggtctgtca
     1681 aaaattaacc gcattgtgca gttctttgcc cagcgtccgc aggtgcagga acgtctgacg
     1741 cagcaaattc ttattgcgct acaaacgctg ctgggcacca ataacgtggc tgtctcgatc
     1801 gacgcggtgc attactgcgt gaaggcgcgt ggcatccgcg atgcaaccag tgccacgaca
     1861 acgacctctc ttggtggatt gttcaaatcc agtcagaata cgcgccacga gtttctgcgc
     1921 gctgtgcgtc atcacaacta accaggcatc aaataaaacg aaaggctcag tcgaaagact
     1981 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     2041 caccttcggg tgggcctttc tgcgtttata atcagcattt gcgatcattc acgcagcgct
     2101 tattcagttg ctcactgcga tgtcataatc atcgctacga gctgtgaaag atgcataaag
     2161 ctcgtacgac gcgttcgctc gtctcctcac ttctcctacg gagcgttctg gacacaacgt
     2221 cgtcttgaag ttgcgattat agattgacag ctagctcagt cctagggatt gtgctagcga
     2281 attcattaaa gaggagaaag gtaccatgca tcaccaccat caccatacgt cctcaactcc
     2341 agttagaact gcttacgtta ccaggatcga acacttctcc gctgcgcaca gattgaactc
     2401 cgtccacctc tcgcctgctg agaacgtcaa gctcttcggt aagtgcaacc acacttccgg
     2461 tcacggtcac aactacaagg tcgaggtgac catcaagggt cagatcaacc cacaatccgg
     2521 catggtcatc aacatcaccg atcttaagaa gactttgcaa gtcgctgtca tggacccttg
     2581 tgaccataga aacttggata tagacgtccc atacttcgag tccagaccct ccactactga
     2641 gaacctcgct gtcttcttgt gggagaatat caagagccac ttgccacctt ccgacgcgta
     2701 cgatttgtac gagatcaagt tgcacgaaac cgacaagaac gttgtcgttt acagaggtga
     2761 ataaccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     2821 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     2881 ttctgcgttt ataatcagca tttgcgatca ttcacgcagc gcttattcag ttgctcactg
     2941 cgatgtcata atcatcgcta cgagctgtga aagatgcata aagctcgtac gacgcgttcg
     3001 ctcgtctcct cacttctcct acggagcgtt ctggacacaa cgtcgtcttg aagttgcgat
     3061 tatagattga cagctagctc agtcctaggg attgtgctag cgaattcatt aaagaggaga
     3121 aaggtaccat gcatcaccat caccaccata gcagtaaaga acatcatttg gttattatta
     3181 acggtgttaa tagaggtttc gggcactccg tcgcgttgga ttacataaga cactcaggtg
     3241 ctcacgcggt gtcctttgtc ttggttggta gaacccagca ttccttggag caagtcttaa
     3301 cggagctgca cgaggctgca tcccacgctg gtgtcgtctt caagggtgtc gttgtgtccg
     3361 aggtcgacct ggctcacttg aactccctcg actccaacct cgcgaggata cagtccgccg
     3421 ccgctgacct aagagacgag gcggcgcaaa gcaccagaac tatcactaag tcggtcctct
     3481 tcaacaacgc gggtagcttg ggtgacttgt ccaagactgt taaggagttc acctggcaag
     3541 aggctcgttc ctacctcgat ttcaacgtcg tgtccctcgt tggtttgtgc tccatgttct
     3601 tgaaggatac cctcgaagca ttcccaaagg aacaataccc agatcataga actgtggtcg
     3661 tgtccatctc ttccctatta gctgttcagg ctttcccaaa ctggggtttg tacgctgctg
     3721 gtaaggcagc tagagataga ctattaggtg ttattgctct cgaagaagca gctaataacg
     3781 taaagacctt gaactacgct ccaggtccat tggataacga aatgcaggct gacgtccgca
     3841 gaactttggg tgataaggaa caactgaaga tctacgacga catgcataag tctggttcct
     3901 tggtgaagat ggaggactcc tctagaaagt tgattcattt gttaaaggct gacaccttca
     3961 cctccggtgg ccacattgat ttctacgacg aataaaaact cgagtaagga tctccaggca
     4021 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     4081 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     4141 tataaaaact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
     4201 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     4261 atggacgcac accgtggaaa cggatgaagg cacgaaccca gttgacataa gcctgttcgg
     4321 ttcgtaaact gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     4381 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     4441 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     4501 tgttatggag cagcaacgat gttacgcagc agcaacgatg ttacgcagca gggcagtcgc
     4561 cctaaaacaa agttaggtgg ctcaagtatg ggcatcattc gcacatgtag gctcggccct
     4621 gaccaagtca aatccatgcg ggctgctctt gatcttttcg gtcgtgagtt cggagacgta
     4681 gccacctact cccaacatca gccggactcc gattacctcg ggaacttgct ccgtagtaag
     4741 acattcatcg cgcttgctgc cttcgaccaa gaagcggttg ttggcgctct cgcggcttac
     4801 gttctgccca ggtttgagca gccgcgtagt gagatctata tctatgatct cgcagtctcc
     4861 ggcgagcacc ggaggcaggg cattgccacc gcgctcatca atctcctcaa gcatgaggcc
     4921 aacgcgcttg gtgcttatgt gatctacgtg caagcagatt acggtgacga tcccgcagtg
     4981 gctctctata caaagttggg catacgggaa gaagtgatgc actttgatat cgacccaagt
     5041 accgccacct aacaattcgt tcaagccgag atcggcttcc cggccgcgga gttgttcggt
     5101 aaattgtcac aacgccgcca ggtggcactt ttcggggaaa tgtgcgcgcc cgcgttcctg
     5161 ctggcgctgg gcctgtttct ggcgctggac ttcccgctgt tccgtcagca gcttttcgcc
     5221 cacggccttg atgatcgcgg cggccttggc ctgcatatcc cgattcaacg gccccagggc
     5281 gtccagaacg ggcttcaggc gctcccgaag gtctcgggcc gtctcttggg cttgatcggc
     5341 cttcttgcgc atctcacgcg ctcctgcggc ggcctgtagg gcaggctcat acccctgccg
     5401 aaccgctttt gtcagccggt cggccacggc ttccggcgtc tcaacgcgct ttgagattcc
     5461 cagcttttcg gccaatccct gcggtgcata ggcgcgtggc tcgaccgctt gcgggctgat
     5521 ggtgacgtgg cccactggtg gccgctccag ggcctcgtag aacgcctgaa tgcgcgtgtg
     5581 acgtgccttg ctgccctcga tgccccgttg cagccctaga tcggccacag cggccgcaaa
     5641 cgtggtctgg tcgcgggtca tctgcgcttt gttgccgatg aactccttgg ccgacagcct
     5701 gccgtcctgc gtcagcggca ccacgaacgc ggtcatgtgc gggctggttt cgtcacggtg
     5761 gatgctggcc gtcacgatgc gatccgcccc gtacttgtcc gccagccact tgtgcgcctt
     5821 ctcgaagaac gccgcctgct gttcttggct ggccgacttc caccattccg ggctggccgt
     5881 catgacgtac tcgaccgcca acacagcgtc cttgcgccgc ttctctggca gcaactcgcg
     5941 cagtcggccc atcgcttcat cggtgctgct ggccgcccag tgctcgttct ctggcgtcct
     6001 gctggcgtca gcgttgggcg tctcgcgctc gcggtaggcg tgcttgagac tggccgccac
     6061 gttgcccatt ttcgccagct tcttgcatcg catgatcgcg tatgccgcca tgcctgcccc
     6121 tcccttttgg tgtccaaccg gctcgacggg ggcagcgcaa ggcggtgcct ccggcgggcc
     6181 actcaatgct tgagtatact cactagactt tgcttcgcaa agtcgtgacc gcctacggcg
     6241 gctgcggcgc cctacgggct tgctctccgg gcttcgccct gcgcggtcgc tgcgctccct
     6301 tgccagcccg tggatatgtg gacgatggcc gcgagcggcc accggctggc tcgcttcgct
     6361 cggcccgtgg acaaccctgc tggacaagct gatggacagg ctgcgcctgc ccacgagctt
     6421 gaccacaggg attgcccacc ggctacccag ccttcgacca catacccacc ggctccaact
     6481 gcgcggcctg cggccttgcc ccatcaattt ttttaatttt ctctggggaa aagcctccgg
     6541 cctgcggcct gcgcgcttcg cttgccggtt ggacaccaag tggaaggcgg gtcaaggctc
     6601 gcgcagcgac cgcgcagcgg cttggccttg acgcgcctgg aacgacccaa gcctatgcga
     6661 gtgggggcag tcgaaggcga agcccgcccg cctgcccccc gagcctcacg gcggcgagtg
     6721 cgggggttcc aagggggcag cgccaccttg ggcaaggccg aaggccgcgc agtcgatcaa
     6781 caagccccgg aggggccact ttttgccgga gggggagccg cgccgaaggc gtgggggaac
     6841 cccgcagggg tgcccttctt tgggcaccaa agaactagat atagggcgaa atgcgaaaga
     6901 cttaaaaatc aacaacttaa aaaagggggg tacgcaacag ctcattgcgg caccccccgc
     6961 aatagctcat tgcgtaggtt aaagaaaatc tgtaattgac tgccactttt acgcaacgca
     7021 taattgttgt cgcgctgccg aaaagttgca gctgattgcg catggtgccg caaccgtgcg
     7081 gcaccctacc gcatggagat aagcatggcc acgcagtcca gagaaatcgg cattcaagcc
     7141 aagaacaagc ccggtcactg ggtgcaaacg gaacgcaaag cgcatgaggc gtgggccggg
     7201 cttattgcga ggaaacccac ggcggcaatg ctgctgcatc acctcgtggc gcagatgggc
     7261 caccagaacg ccgtggtggt cagccagaag acactttcca agctcatcgg acgttctttg
     7321 cggacggtcc aatacgcagt caaggacttg gtggccgagc gctggatctc cgtcgtgaag
     7381 ctcaacggcc ccggcaccgt gtcggcctac gtggtcaatg accgcgtggc gtggggccag
     7441 ccccgcgacc agttgcgcct gtcggtgttc agtgccgccg tggtggttga tcacgacgac
     7501 caggacgaat cgctgttggg gcatggcgac ctgcgccgca tcccgaccct gtatccgggc
     7561 gagcagcaac taccgaccgg ccccggcgag gagccgccca gccagcccgg cattccgggc
     7621 atggaaccag acctgccagc cttgaccgaa acggaggaat gggaacggcg cgggcagcag
     7681 cgcctgccga tgcccgatga gccgtgtttt ctggacgatg gcgagccgtt ggagccgccg
     7741 acacgggtca cgctgccgcg ccggtagcac ttgggttgcg cagcaacccg taagtgcgct
     7801 gttccagact atcggctgta gccgcctcgc cgccctatac cttgtctgcc tccccgcgtt
     7861 gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct
     7921 aacggattca ccgtttttat caggctctgg gaggcagaat aaatgatcat atcgtcaatt
     7981 attacctcca cggggagagc ctgagcaaac tggcctcagg catttgagaa gcacacggtc
     8041 acactgcttc cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa
     8101 cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt cgaatttctg ccattcatcc
     8161 gcttattatc acttattcag gcgtagcacc aggcgtttaa gggcaccaat aactgcctta
     8221 aaaaaattac gccccgccct gccactcatc gcagtcggcc tattggttaa aaaatgagct
     8281 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgc
//
