LOCUS       Exported                7210 bp ds-DNA     circular SYN 10-MAY-2021
DEFINITION  Alternative names: pCK306, pGNW2-pp2_J3(106)-BBa_J23117-sfGFP
            Single cross-over into P. putida. Then, kanamycin resistant marker 
            can be flipped out by pCK255.
ACCESSION   .
VERSION     .
KEYWORDS    pPPC034
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 10, 2021 from SnapGene Viewer 5.2.3
            https://www.snapgene.com
COMMENT     Alias: pCK306
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     promoter        1..31
                     /label=lac promoter
                     /note="color: #84b0dc"
     protein_bind    39..55
                     /label=lac operator
                     /note="color: #75c6a9"
     primer_bind     44..66
                     /label=M13/pUC Reverse
                     /note="color: #b1ff67; direction: RIGHT"
     primer_bind     63..79
                     /label=M13 Reverse
                     /note="color: #85dae9; direction: RIGHT"
     primer_bind     63..79
                     /label=M13 rev
                     /note="color: #85dae9; direction: RIGHT"
     misc_feature    75..93
                     /label=Seq-pGNW_R
                     /note="color: #b4abac"
     misc_feature    complement(94..111)
                     /label=I-SceI
                     /note="color: #c7b0e3; direction: LEFT"
     misc_feature    complement(111..128)
                     /label=pGNW-USER_F
                     /note="color: #ff9ccd; direction: LEFT"
     misc_feature    142..1141
                     /label=PP2_HR1
                     /note="color: #84b0dc"
     misc_feature    142..632
                     /label=PP_3038
                     /note="color: #c7b0e3; direction: RIGHT"
     gene            629..991
                     /label=PP_3039 gene
                     /note="color: #c6c9d1; direction: RIGHT"
     CDS             629..991
                     /label=PP_3039 CDS (conserved protein of unknown funct
                     /label=PP_3039 CDS (conserved protein of unknown function)
                     /note="color: #84b0dc; direction: RIGHT"
     misc_feature    1142..1147
                     /label=Mph1103I
                     /note="color: #9eafd2"
     misc_feature    1148..1317
                     /label=J3
                     /note="color: #85dae9; direction: RIGHT"
     misc_feature    1253..1271
                     /label=J309
                     /note="color: #b7e6d7"
     misc_feature    complement(1272..1291)
                     /label=J106
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    1318..1352
                     /label=BBa_J23111
                     /note="color: #ffef86; direction: RIGHT"
     misc_feature    1353..1379
                     /label=Bujard RBS (strong)
                     /note="color: #db7aff"
     RBS             1362..1373
                     /label=RBS (strong)
                     /note="color: #996633"
     misc_feature    1380..2096
                     /label=sfGFP
                     /note="color: #b1ff67; direction: RIGHT"
     misc_feature    complement(1418..1437)
                     /label=jfGFP1
                     /note="color: #b1ff67; direction: LEFT"
     terminator      2121..2249
                     /label=dblTerm
                     /note="color: #800080; direction: RIGHT"
     misc_feature    2250..2255
                     /label=ScaI
                     /note="color: #84b0dc"
     misc_feature    2256..3255
                     /label=PP2_HR2
                     /note="color: #ffef86"
     gene            2456..2803
                     /label=PP_3040 gene
                     /note="color: #ffef86; direction: RIGHT"
     CDS             2456..2803
                     /label=PP_3040 CDS (Pyocin R2_PP, holin)
                     /note="color: #d59687; direction: RIGHT"
     misc_feature    2968..3255
                     /label=PP_3041
                     /note="color: #ffef86; direction: RIGHT"
     misc_feature    complement(3263..3280)
                     /label=I-SceI
                     /note="color: #c7b0e3; direction: LEFT"
     misc_feature    complement(3282..3299)
                     /label=Seq-pGNW_F
                     /note="color: #ff9ccd; direction: LEFT"
     primer_bind     complement(3282..3299)
                     /label=M13 Forward
                     /note="color: #f8d3a9; direction: LEFT"
     primer_bind     complement(3282..3298)
                     /label=M13 fwd
                     /note="color: #ffef86; direction: LEFT"
     primer_bind     complement(3291..3313)
                     /label=M13/pUC Forward
                     /note="color: #84b0dc; direction: LEFT"
     CDS             complement(3730..4101)
                     /label=traJ
                     /note="color: #b4abac; direction: LEFT"
     oriT            4134..4243
                     /label=incP origin of transfer
                     /note="color: #75c6a9"
     rep_origin      4403..4791
                     /label=R6K gamma ori
                     /note="color: #ff9ccd"
     terminator      5016..5110
                     /label=lambda t0 terminator
                     /note="color: #c7b0e3"
     misc_feature    complement(5127..5840)
                     /label=msfGFP
                     /note="color: #b1ff67; direction: LEFT"
     CDS             complement(5127..5840)
                     /label=Translation 5840-5127
                     /note="color: #993366; direction: LEFT"
     CDS             6106..6900
                     /label=NeoR/KanR
                     /note="color: #d6b295; direction: RIGHT"
     primer_bind     complement(6160..6179)
                     /label=Neo-R
                     /note="color: #faac61; direction: LEFT"
     primer_bind     6770..6789
                     /label=Neo-F
                     /note="color: #d6b295; direction: RIGHT"
     protein_bind    7175..7196
                     /label=CAP binding site
                     /note="color: #84b0dc"
ORIGIN
        1 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
       61 cacaggaaac agctatgacc atgattacgc cggattaccc tgttatccct agaagcttgc
      121 atgcctgcag gtcgactcta gaccaggatg aataccttaa ggacgccacc ggtaaccggc
      181 gttattggcc ggtcgcttgc gtcaaggtgg accttgaagc attgcgtcgc gctcgtgacc
      241 agctgtgggc tgaggccatg ttctgctacc aggccggtga tatctggtgg gtgacccgtg
      301 aggaggaaga actgttcact gcagagcagg aagagcgctt cgtggtagat gaatgggagg
      361 ggccgatcct gaaatggttg gaggaatccc aggccggcga gacggtcacc ggaagcgaag
      421 tgttggggca ggcattgaac cttgaccctg gccactgggg caagcctgag cagatgcggg
      481 tgggatcgat catgcaccgc ctaggttggc ggcgtcgcag gctggctgcg ctgccgaaga
      541 gcggtaagcg cccttgggca tatcagaagc ctgatggttg ggggcgcagc gccttggagc
      601 agtccacgca gccgaaggag gagtgctttt gatcaaacac atagatgaga tgctgaaact
      661 gtgggctcaa gagctccatg cgccggagcc ctgtcattcg gcgggcggtg ttggtagcat
      721 gctcggcctg ttgatcgagt gcaagggtga ccttgtgcgt ggcacccgag gcagcaaggt
      781 gctactggac gagtctgcgg acatcgagat cattgtgaat aagcatctgg caccggagct
      841 ctacctggtg gttcgtgagc actactgtaa cgccgacagc gagctgtacc aaaagtaccg
      901 gcactgtggg tgtagccggg atacctatta caagcgctta catgaggcgc acgtctgtat
      961 cgcaggcttg ctcttggggc gagcggcatg attcgccatc cacggtccta ccgtcccgct
     1021 gccgtcctgc cgcgtttgat gcaggtcagc ctagctcaag cccgcgcagg tcgcgggctg
     1081 tcccaccgtc ccaccgtaca cacgaaggcg cgcacatagg cgtgtgcagc gcatcacgcg
     1141 catgcatagc atttgcgatc attcacgcag cgcttattca gttgctcact gcgatgtcat
     1201 aatcatcgct acgagctgtg aaagatgcat aaagctcgta cgacgcgttc gctcgtctcc
     1261 tcacttctcc tgcggttacc aaaggcgtcc tcgtcgtctt gaagttgcga ttatagattg
     1321 acggctagct cagtcctagg tatagtgcta gcgaattcat taaagaggag aaaggtacca
     1381 tgagcaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
     1441 atgttaatgg gcacaaattt tctgtccgtg gagagggtga aggtgatgct acaaacggaa
     1501 aactcaccct taaatttatt tgcactactg gaaaactacc tgttccgtgg ccaacacttg
     1561 tcactactct gacctatggt gttcaatgct tttcccgtta tccggatcac atgaaacggc
     1621 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaacgcact atatctttca
     1681 aagatgacgg gacctacaag acgcgtgctg aagtcaagtt tgaaggtgat acccttgtta
     1741 atcgtatcga gttaaagggt attgatttta aagaagatgg aaacattctt ggacacaaac
     1801 tcgagtacaa ctttaactca cacaatgtat acatcacggc agacaaacaa aagaatggaa
     1861 tcaaagctaa cttcaaaatt cgccacaacg ttgaagatgg ttccgttcaa ctagcagacc
     1921 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc
     1981 tgtcgacaca atctgtcctt tcgaaagatc ccaacgaaaa gcgtgaccac atggtccttc
     2041 ttgagtttgt aactgctgct gggattacac atggcatgga tgagctctac aaataaggat
     2101 ccaaactcga gtaaggatct ccaggcatca aataaaacga aaggctcagt cgaaagactg
     2161 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     2221 accttcgggt gggcctttct gcgtttataa gtactaaggt gtattccccc ggcattcaac
     2281 agacattccc cggacgttta ccacttattg ccgggtggca ttaaaactcg cttgctgcca
     2341 ccggaatcga cctgtaaaaa gtacccatct tcgagacgtg cgggcgcaca aagcggcccg
     2401 ccaaacactg aaaaccccgg ccctggcgcc ggggtttttg cctttggggg attcgatgaa
     2461 cagcgagcag caagcgttag ttgagatgcc aatctggttg gtgatcttcc tgtccctggt
     2521 cggcggggtg tcaggcgaga tgtggcgggc cgacatggcc ggcgttagcg gctggttcat
     2581 tttccgccag gtgctgctgc gctccggtgc ctgcgtcgta tgcggactgt cgaccatcat
     2641 gctgctgtac tcggcgggca tgtcgatgtg gtcggccagt gccattggtt gtctcactgc
     2701 cactgccggt gcggatgtgg ccatagggtt gtacaagcgt tgggtcgcca agcggctggg
     2761 cgtctgcgat gtcacgtccc gtagcggcga acctggacag tgacccgatc gccagcctcg
     2821 gtggggtcgg ggaccctggc gatatggccg ggttacgggg caggaaaccc gcggctcttc
     2881 gctagcggac agttcgccag cttactgaaa ttcaacctgt tgaaattgaa aggttgttgg
     2941 ttgaaatacc attgaaatgg agggctcatg acggattcga acttcttgtc aaagagcgcc
     3001 ttcgctgctc gcatagggag atcacccagc tacatcacct ggttgaaaga caacggccgc
     3061 ctggtgcttt cacccgatgg aaaattggtg gatgtgctgg ccaccgaggc caagattcag
     3121 gagacagctg atccggccaa agcagccgtc gcggctcggc atgaagaaaa ccgcatcgag
     3181 cgggacgtcc gggcccacat ccagcctagc gccgacacac ctgcggtgca gccagcggat
     3241 cacgcgccga gcggaaattc agattaccct gttatcccta tactggccgt cgttttacaa
     3301 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     3361 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     3421 agcctgaatg gcgaatgggg ctcgatcccc tcgcgagttg gttcagctgc tgcctgaggc
     3481 tggacgacct cgcggagttc taccggcagt gcaaatccgt cggcatccag gaaaccagca
     3541 gcggctatcc gcgcatccat gcccccgaac tgcaggagtg gggaggcacg atggccgctt
     3601 tggtcggtct tgccttgctc gtcggtgatg tacttcacca gctccgcgaa gtcgctcttc
     3661 ttgatggagc gcatggggac gtgcttggca atcacgcgca ccccccggcc gttttagcgg
     3721 ctaaaaaagt catggctctg ccctcgggcg gaccacgccc atcatgacct tgccaagctc
     3781 gtcctgcttc tcttcgatct tcgccagcag ggcgaggatc gtggcatcac cgaaccgcgc
     3841 cgtgcgcggg tcgtcggtga gccagagttt cagcaggccg cccaggcggc ccaggtcgcc
     3901 attgatgcgg gccagctcgc ggacgtgctc atagtccacg acgcccgtga ttttgtagcc
     3961 ctggccgacg gccagcaggt aggccgacag gctcatgccg gccgccgccg ccttttcctc
     4021 aatcgctctt cgttcgtctg gaaggcagta caccttgata ggtgggctgc ccttcctggt
     4081 tggcttggtt tcatcagcca tccgcttgcc ctcatctgtt acgccggcgg tagccggcca
     4141 gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc gaataaggga cagtgaagaa
     4201 ggaacacccg ctcgcgggtg ggcctacttc acctatcctg cccggctgac gccgttggat
     4261 acaccaagga aagtctacac gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga
     4321 tggatatacc gaaaaaatcg ctataatgac cccgaagcag ggttatgcag cggaaaacgg
     4381 ccacgatgcg tccggcgtag aggatctgaa gatcagcagt tcaacctgtt gatagtacgt
     4441 actaagctct catgtttcac gtactaagct ctcatgttta acgtactaag ctctcatgtt
     4501 taacgaacta aaccctcatg gctaacgtac taagctctca tggctaacgt actaagctct
     4561 catgtttcac gtactaagct ctcatgtttg aacaataaaa ttaatataaa tcagcaactt
     4621 aaatagcctc taaggtttta agttttataa gaaaaaaaag aatatataag gcttttaaag
     4681 cttttaaggt ttaacggttg tggacaacaa gccagggatg taacgcactg agaagccctt
     4741 agagcctctc aaagcaattt tgagtgacac aggaacactt aacggctgac atgggaatta
     4801 gcttcacgct gccgcaagca ctcagggcgc aagggctgct aaaggaagcg gaacacgtag
     4861 aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg tcagctactg ggctatctgg
     4921 acaagggaaa acgcaagcgc aaagagaaag caggtagctg taacatcaga gattttgaga
     4981 cacaaattta aatcgtaatt attggggacc cctggattct caccaataaa aaacgcccgg
     5041 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
     5101 aacaggagtc caagactagt catttatttg tagagttcat ccatgccgtg cgtgatacct
     5161 gcggcagtaa cgaactccag cagcaccatg tggtcgcgct tttcgttcgg gtctttggac
     5221 agtttagact gggtggacag gtagtggtta tccggcagca gaaccggacc atcaccgatc
     5281 ggagtgttct gctggtagtg gtccgccagc tgtacgctac cgtcttcaac gttatggcga
     5341 attttgaagt tagctttgat accgttcttc tgtttgtctg cggtgatgta aacgttatgg
     5401 gagttgaagt tatattccag tttgtggccc aggatgttgc cgtcctcttt gaaatcaatg
     5461 cctttcagtt caatacggtt caccagagta tcaccttcaa atttaacctc tgcacgggtt
     5521 ttgtaggtgc catcgtcttt gaaagaaatg gtgcgctcct gtacataacc ttccggcatt
     5581 gcagatttga agaaatcatg ctgcttcatg tgatccgggt aacgagaaaa acactgaaca
     5641 ccataggtca gggtagtcac cagagtcggc catggaaccg gcagtttacc ggtagtgcag
     5701 atgaatttca gggtcagttt accgttggtt gcatcacctt caccttcacc acgaacagag
     5761 aatttgtggc cgttaacatc accatccagt tcaaccagga tcggaacaac accggtgaac
     5821 agttcttcac ctttacgcat atgtttttcc tcctaaacat gcgtcgcggc ctaggtcgtg
     5881 caattatacc tggccgcgag agccttgtca atgggcttaa ttaaatggcg atagctagac
     5941 tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag
     6001 gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc
     6061 aggggatcaa gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     6121 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     6181 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     6241 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     6301 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     6361 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     6421 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     6481 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     6541 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     6601 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     6661 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     6721 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     6781 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     6841 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     6901 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     6961 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     7021 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccccaatac
     7081 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     7141 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     7201 caccccaggc
//
