LOCUS       pAAV-U6-LacZ_#1,_2-hSyn 5657 bp ds-DNA     circular     06-MAY-2021
DEFINITION  .
KEYWORDS    "creator:Michael Reed" "marker:L-ITR, R-ITR, AmpR"                 
FEATURES             Location/Qualifiers
     primer          174..195
                     /label="pUC forward"
                     /note="sequence: ggagcctatggaaaaacgccag"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    249..389
                     /label="L-ITR"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     misc_feature    399..403
                     /label="MluI"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     promoter        404..652
                     /label="Human U6 Promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    653..757
                     /label="LacZ #1"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        763..1011
                     /label="Human U6 Promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1012..1116
                     /label="LacZ #2"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1117..1121
                     /label="chimeric RNA backbone 1 (SapI)"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     misc_feature    1122..1127
                     /label="XbaI"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     misc_feature    1122..1603
                     /label="hSyn"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     primer          complement(1190..1209)
                     /label="hSyn reverse"
                     /note="sequence: gtcggtcgtcaggtaggcac"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          1536..1554
                     /label="hSyn forward"
                     /note="sequence: ctcagcgctgcctcagtct"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1631..2335
                     /label="mCherry"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             2360..2608
                     /label="KASH domain of Nesprin2"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2617..2630
                     /label="WPRE"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     polyA_signal    2650..3128
                     /label="hGH polyA"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     misc_feature    3168..3308
                     /label="R-ITR"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     rep_origin      3400..3706
                     /label="f1 Origin"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4225..5082
                     /label="AmpR"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     rep_origin      join(5233..5657,1..243)
                     /label="pUC ori"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
       61 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      121 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      181 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      241 tcacatgtcc tgcaggcagc tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc
      301 cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg
      361 agtggccaac tccatcacta ggggttcctg cggccgcaCG CGTgagggcc tatttcccat
      421 gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt
      481 gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg
      541 gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg
      601 aaagtatttc gatttcttgg ctttatatat cttGTGGAAA GGACGAAACA CCGTGTTCGC
      661 ATTATCCGAA CCATgtttta gagctaGAAA tagcaagtta aaataaggct agtccgttat
      721 caacttgaaa aagtggcacc gagtcggtgc TTTTTTGCTA GCgagggcct atttcccatg
      781 attccttcat atttgcatat acgatacaag gctgttagag agataattgg aattaatttg
      841 actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg
      901 tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga
      961 aagtatttcg atttcttggc tttatatatc ttGTGGAAAG GACGAAACAC CGCGCGATCG
     1021 TAATCACCCG AGTgttttag agctaGAAAt agcaagttaa aataaggcta gtccgttatc
     1081 aacttgaaaa agtggcaccg agtcggtgcT TTTTTACCGG Ttctagactg cagagggccc
     1141 tgcgtatgag tgcaagtggg ttttaggacc aggatgaggc ggggtggggg tgcctacctg
     1201 acgaccgacc ccgacccact ggacaagcac ccaaccccca ttccccaaat tgcgcatccc
     1261 ctatcagaga gggggagggg aaacaggatg cggcgaggcg cgtgcgcact gccagcttca
     1321 gcaccgcgga cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg cctcagcact
     1381 gaaggcgcgc tgacgtcact cgccggtccc ccgcaaactc cccttcccgg ccaccttggt
     1441 cgcgtccgcg ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga gatagggggg
     1501 cacgggcgcg accatctgcg ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg
     1561 ggcagcggag gagtcgtgtc gtgcctgaga gcgcagtcga gaaggtaccg gatcctctag
     1621 agTCGACatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
     1681 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     1741 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     1801 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
     1861 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
     1921 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     1981 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     2041 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
     2101 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
     2161 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
     2221 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     2281 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagGAATT
     2341 Ctccggactc agatctcgag aggaggagga ggagacagac agcaggatgc cccacctcga
     2401 cagccccggc agctcccagc cgagacgctc cttcctctca agggtgatca gggcagcgct
     2461 accgttgcag ctgcttctgc tgctgctgct gctcctggcc tgcctgctac ctgcctctga
     2521 agatgactac agctgcaccc aggccaacaa ctttgcccga tccttctacc ccatgctgcg
     2581 gtacaccaac gggccacctc ccacctagGA Tatcaagctt atcgataccg agcgctgctc
     2641 gagagatcta cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag
     2701 ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg
     2761 actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag
     2821 ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg
     2881 cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc
     2941 ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt
     3001 ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga
     3061 tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc
     3121 ctgtccttct gattttgtag gtaaccacgt gcggaccgag cggccgcagg aacccctagt
     3181 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
     3241 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg
     3301 cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     3361 catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     3421 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     3481 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     3541 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
     3601 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     3661 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     3721 tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     3781 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta
     3841 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     3901 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     3961 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     4021 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     4081 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4141 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4201 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4261 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     4321 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     4381 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     4441 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     4501 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     4561 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     4621 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     4681 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4741 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     4801 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     4861 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     4921 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     4981 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5041 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5101 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5161 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5221 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5281 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5341 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5401 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5461 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5521 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5581 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5641 cgtgagctat gagaaag
//