LOCUS       Exported                8791 bp ds-DNA     circular SYN 13-JAN-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8791)
  AUTHORS   AAP
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 6, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8791
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        3..80
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             81..1163
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     misc_feature    1406..1434
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1442..1458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1528..2628
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     2593..2616
                     /label=AAPS1
     CDS             2677..2694
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2695..2715
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(2707..2724)
                     /label=AAP70
     CDS             2725..4806
                     /codon_start=1
                     /label=PGL-3
                     /translation="MEANKRQIVEVDGIKSYFFPHLAHYLASNDELLVNNIAQANKLAA
                     FVLGATDKRPSNEEIAEMILPNDSSAYVLAAGMDVCLILGDDFRPKFDSGAEKLSQLGQ
                     AHDLAPIIDDEKKISMLARKTKLKKSNDAKILQVLLKVLGAEEAEEKFVELSELSSALD
                     LDFDVYVLAKLLGFASEELQEEIEIIRDNVTDAFEACKPLLKKLMIEGPKIDSVDPFTQ
                     LLLTPQEESIEKAVSHIVARFEEASAVEDDESLVLKSQLGYQLIFLVVRSLADGKRDAS
                     RTIQSLMPSSVRAEVFPGLQRSVFKSAVFLASHIIQVFLGSMKSFEDWAFVGLAEDLES
                     TWRRRAIAELLKKFRISVLEQCFSQPIPLLPQSELNNETVIENVNNALQFALWITEFYG
                     SESEKKSLNQLQFLSPKSKNLLVDSFKKFAQGLDSKDHVNRIIESLEKSSSSEPSATAK
                     QTTTSNGPTTVSTAAQVVTVEKMPFSRQTIPCEGTDLANVLNSAKIIGESVTVAAHDVI
                     PEKLNAEKNDNTPSTASPVQFSSDGWDSPTKSVALPPKISTLEEEQEEDTTITKVSPQP
                     QERTGTAWGSGDATPVPLATPVNEYKVSGFGAAPVASGFGQFASSNGTSGRGSYGGGRG
                     GDRGGRGAYGGDRGRGGSGDGSRGYRGGDRGGRGSYGEGSRGYQGGRAGFFGGSRGGS"
     primer_bind     3337..3356
                     /label=AAP69
     primer_bind     complement(3343..3360)
                     /label=AAP65
     primer_bind     complement(4042..4065)
                     /label=AAP67
     primer_bind     4066..4083
                     /label=AAP66
     primer_bind     complement(4573..4590)
                     /label=AAP68
     primer_bind     4804..4827
                     /label=AAP64
     protein_bind    complement(4807..4831)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4882..4898)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      5247..5333
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5425..5452
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        5471..5562
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5563..6423
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      complement(6465..6978)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      7089..7677
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    7863..8005
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(8107..8298)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
       61 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
      121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
      181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
      241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
      301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
      361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
      421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
      481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
      541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
      601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
      661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
      721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
      781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
      841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
      901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
      961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     1141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag
     1201 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg
     1261 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg
     1321 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt
     1381 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga
     1441 attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga
     1501 gcacttcacc aacaaggacc atagcatatg aaaatcgaag aaggtaaact ggtaatctgg
     1561 attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat
     1621 accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt
     1681 gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac
     1741 gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat
     1801 ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt
     1861 gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa
     1921 gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac
     1981 ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag
     2041 tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg
     2101 ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac
     2161 tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg
     2221 gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc
     2281 aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt
     2341 ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg
     2401 gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag
     2461 ttggcgaaag atccacgtat tgccgccact atggaaaacg cccagaaagg tgaaatcatg
     2521 ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc
     2581 gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg
     2641 aacaacaaca acaataacaa taacaacaac ctcgggcacc accaccacca ccacgaaaac
     2701 ctgtacttcc aaggcgcagg cttaatggaa gcaaacaaac gacaaattgt ggaggtcgat
     2761 gggattaagt cctacttttt cccccatctc gcccattacc tcgcgagcaa tgacgagctg
     2821 ttggtcaaca acatagcaca ggcgaacaag cttgcagctt tcgtcttggg agccaccgac
     2881 aaacgcccaa gcaatgaaga aattgcggag atgatcctgc caaatgactc cagtgcctac
     2941 gtgcttgctg ctggcatgga cgtttgcctt attctcgggg atgacttccg accaaagttc
     3001 gattctggcg ctgagaagct gagtcagctt ggacaagctc atgatttggc tccaatcatc
     3061 gatgatgaaa agaaaatctc gatgctcgcc cgaaaaacaa agctaaagaa gtcgaatgac
     3121 gccaagattc tgcaagtctt gctcaaagtt ctcggtgcgg aagaagcgga agagaagttt
     3181 gtcgagctct cggaactgtc gagcgccttg gatcttgatt tcgacgtcta tgtcttggca
     3241 aaactgcttg gatttgcatc ggaggagctc caggaagaaa ttgaaatcat ccgtgacaac
     3301 gtcacagatg cgtttgaggc ctgcaagccg ttgttgaaga agctgatgat cgagggaccg
     3361 aagattgatt ctgtggaccc attcacccaa cttctgttaa ctccacaaga agaatctatc
     3421 gaaaaagctg tatcccacat cgttgctcgc ttcgaagaag ccagcgccgt cgaggatgat
     3481 gaatcactcg tcctgaaatc tcagctagga tatcaattga ttttcctcgt cgttcgctca
     3541 cttgctgatg gaaagcgtga tgcttccagg acaatccaat cgttgatgcc gtcgagtgtc
     3601 cgagcggaag tgtttcccgg attgcaacgg agtgttttca aaagtgccgt gttcctcgca
     3661 agccatatca ttcaagtctt tttgggatcc atgaagtcat tcgaggattg ggcgtttgtt
     3721 ggcctcgctg aagacttgga gagcacgtgg agaagaagag caatcgctga attgctgaaa
     3781 aagttcagaa tctctgttct cgagcagtgc ttttctcaac caatcccact gctccctcaa
     3841 agcgagttga acaacgaaac tgtcatcgag aatgtgaata atgctctcca atttgcattg
     3901 tggatcacag agttctacgg atcggagagt gagaagaaat cactcaatca gcttcaattc
     3961 ctaagcccaa aatccaagaa tcttcttgtt gatagcttca aaaagttcgc ccaaggactg
     4021 gattctaaag atcacgtgaa tcgtatcatt gaatctctgg agaagagcag ttcgtcggaa
     4081 ccttctgcaa ctgcaaaaca gaccacaact tccaatggac cgaccaccgt ctctactgca
     4141 gctcaagtag taactgttga aaagatgcca ttcagtcgtc aaacgattcc atgtgaagga
     4201 acagacttgg cgaatgtgct caattctgca aagatcatcg gagaaagtgt taccgtggct
     4261 gcccatgatg tcattccgga gaaactaaat gcggagaaga atgacaatac tccatcaact
     4321 gcctcacctg ttcaattttc tagcgatggt tgggattctc cgacaaaatc tgttgctctg
     4381 ccaccgaaga tctcgacttt ggaggaagaa caagaagaag atacaacgat caccaaggtt
     4441 tctccgcaac cacaagaacg aactggaacc gcatggggat caggcgatgc gactccagtg
     4501 cctcttgcta ctccggttaa cgaatacaaa gttagtgggt tcggcgctgc gccagtcgct
     4561 tcgggattcg gacagtttgc cagcagcaac ggaacgtctg gaagaggctc ttatggaggt
     4621 ggtcgcggtg gcgatcgtgg aggaagagga gcatacggtg gcgatcgtgg acgtggaggt
     4681 tccggagatg gatctcgtgg atatcgtggt ggtgatcgag gaggacgcgg aagttacggc
     4741 gaaggatctc gtggttacca gggaggacgt gccggattct ttggtggatc ccgtggaggt
     4801 tcctaaaccc agctttcttg tacaaagtgg tgatatttca gaattcggat cctctagagt
     4861 cgacctgcag gcaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     4921 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     4981 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggca
     5041 gcttggctgt tttggcggat gagataagat tttcagcctg atacagatta aatcagaacg
     5101 cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga
     5161 ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca
     5221 tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     5281 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg
     5341 gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat
     5401 aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc
     5461 tacaaactct tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     5521 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     5581 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     5641 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     5701 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg tttcccaatg
     5761 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     5821 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     5881 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     5941 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     6001 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     6061 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     6121 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     6181 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     6241 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     6301 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     6361 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     6421 taactgtcag accaagttta ctcatatata ctttagattg atttaccccg gttgataatc
     6481 agaaaagccc caaaaacagg aagattgtat aagcaaatat ttaaattgta aacgttaata
     6541 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     6601 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     6661 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     6721 ccgtctatca gggcgatggc ccactacgtg aaccatcacc caaatcaagt tttttggggt
     6781 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     6841 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     6901 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     6961 cgccgctaca gggcgcgtaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     7021 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     7081 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     7141 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     7201 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     7261 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     7321 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     7381 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     7441 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     7501 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     7561 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7621 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7681 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     7741 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     7801 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     7861 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt
     7921 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
     7981 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
     8041 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     8101 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
     8161 atcagcgtgg tcgtgcagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
     8221 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
     8281 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat
     8341 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg
     8401 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa
     8461 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag
     8521 ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt
     8581 ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga
     8641 cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc
     8701 agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac
     8761 ccgtggccag gacccaacgc tgcccgaaat t
//
