LOCUS       Exported                3744 bp ds-DNA     circular SYN 07-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pFTK001 CP0_23_Pc_Pact
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Carsten Pohl
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 7, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     CDS             215..1225
                     /codon_start=1
                     /label=Sm/Sp no DraIII
                     /note="Sm/Sp no DraIII"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     variation       1192
                     /label=G to A
                     /note="G to A"
     misc_feature    1385..1999
                     /label=rep
                     /note="rep"
     rep_origin      1984
                     /label=ORI
                     /note="ORI"
     misc_feature    2236..3728
                     /label=pActin
                     /note="pActin"
     misc_feature    3729..3732
                     /label=AATG
                     /note="AATG"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
      361 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
      421 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
      481 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
      541 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
      601 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
      661 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
      721 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
      781 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
      841 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
      901 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
      961 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     1021 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     1081 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     1141 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga
     1201 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat
     1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2161 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact
     2221 ctgtggtctc aggagggagg ctctggtcga ttcgcgcggc gaccagtggg ttcgatcatg
     2281 tgaccgtata acaaaccctt ggaaaaggaa ctggactaca tcatcgacaa tatagaatac
     2341 gctttctcca gaaggtgata catcataaaa gaaatccatg gttttcatcc caacattgag
     2401 ctacaaaccc tgtcaaccat atgtgattcg agtcttggcc catgtaatac caactgaggt
     2461 ttaatccata ttcgttctca ttggaaagag actgagggct tctagatttt tcatggagat
     2521 aaccgagtta agaaggtatg aaagcatgtt atcaccacgt tttgtttcgg tttgaatggt
     2581 gctggtttgc tggaaccgtt gggcatccgg gatagctttg cagataggaa taatctggcc
     2641 ctgtccggga atagctgtgt tatactatga gatgactgcc atgttcatcg cttccctaat
     2701 ggtttatcga ggtagggagt gccttggaat gtactagaac ctgcccaggt ggctatcaaa
     2761 gttgtgccaa gatatatttc aaggggctta aagttcagtg cgggagaact ttaggtgccg
     2821 atggaggggt caaatactcc gtacatagca ggatgttctg ctagccaaaa gtaggctgac
     2881 ctattccatt ctaatgcaag atcagaacag aacataaaat aaggcaaagt cagtggtgaa
     2941 tagagagaac gtacaagtcg tattcgtact tctcaatgta tatcacgtgg cacttcaccg
     3001 cctcatcatt ccaagaggct tagctgttct ccctgaccct ccagggttgg aaatggcggt
     3061 aacctgaaca gatagaaatg gtctgacagt ggccagggac tctcccgtat tatccccatc
     3121 ttaccaaaac caaaaccgag gtggtttgcg tgtccacttc caataacctt ggaacgggta
     3181 ccccaatgac ttcaagtctg ggtctgtcct atacttagaa catcgacgcc acgggggaat
     3241 tcagcagcct acggagtcca taagacacca agacacagcc attgtatgga ttatatatgc
     3301 catgtatgcc tgacaatgct gtataagtac tgtaatacaa ggtaaacccc caacccggtc
     3361 aaggtacgtg ttcccgccgt acccaaaagg gtccccaaga atgtccacgc aatactttta
     3421 ggtagacatt gaaggaatcc aagtgagaaa ttcaatgaac atgaacaata gttctgcctt
     3481 ataatcttta taagtataaa aatcagaaag agaattatat acaaaagggt agatctggag
     3541 ggggttcaga gttaaggcct caggcagcgc acaatcccag ccatcacaaa cccctctcca
     3601 ctcttccctc tctctctctt ccttcttcct ttctccccta atcccaacta tatcccctct
     3661 aacctctttc catctttctt ttcttttttc ccctcttctc ccctaagtcc cttgtttaat
     3721 cagtcacaaa tgtgagacca cgaa
//
