LOCUS       Exported                3184 bp ds-DNA     circular SYN 07-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pFTK020 CP0_41_palcA-syn
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Carsten Pohl
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 7, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     CDS             215..1225
                     /codon_start=1
                     /label=Sm/Sp no DraIII
                     /note="Sm/Sp no DraIII"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     variation       1192
                     /label=G to A
                     /note="G to A"
     misc_feature    1385..1999
                     /label=rep
                     /note="rep"
     rep_origin      1984
                     /label=ORI
                     /note="ORI"
     misc_feature    2232..3172
                     /label=PalcA synth Long
                     /note="PalcA synth Long"
     misc_feature    2466
                     /label=c-->g
                     /note="c-->g"
     misc_feature    2557
                     /label=g-->c
                     /note="g-->c"
     misc_feature    2571
                     /label=a-->c, removal BpiI-site
                     /note="a-->c, removal BpiI-site"
     misc_feature    2591..2595
                     /label=alcR-binding-site
                     /note="alcR-binding-site"
     misc_feature    2598..2602
                     /label=alcR-binding-site
                     /note="alcR-binding-site"
     misc_feature    2636..2640
                     /label=alcR-binding-site
                     /note="alcR-binding-site"
     misc_feature    2643..2647
                     /label=alcR-binding-site
                     /note="alcR-binding-site"
     misc_feature    2686..2690
                     /label=alcR-binding-site
                     /note="alcR-binding-site"
     misc_feature    2693..2697
                     /label=alcR-binding-site
                     /note="alcR-binding-site"
     misc_feature    2715
                     /label=c-->t
                     /note="c-->t"
     misc_feature    2731..2735
                     /label=alcR-binding-site
                     /note="alcR-binding-site"
     misc_feature    2738..2742
                     /label=alcR-binding-site
                     /note="alcR-binding-site"
     misc_feature    2751
                     /label=g-->t
                     /note="g-->t"
     misc_feature    2770..2771
                     /label=cc-->ta
                     /note="cc-->ta"
     misc_feature    2781..2785
                     /label=alcR-binding-site
                     /note="alcR-binding-site"
     misc_feature    2788..2792
                     /label=alcR-binding-site
                     /note="alcR-binding-site"
     misc_feature    2812..3092
                     /label=core_PalcA
                     /note="core_PalcA"
     misc_feature    2836..2840
                     /label=alcR-binding-site
                     /note="alcR-binding-site"
     misc_feature    2848..2852
                     /label=alcR-binding-site
                     /note="alcR-binding-site"
     misc_feature    2886..2890
                     /label=alcR-binding-site
                     /note="alcR-binding-site"
     misc_feature    2893..2897
                     /label=alcR-binding-site
                     /note="alcR-binding-site"
     misc_feature    2907..2908
                     /label=gg-->at
                     /note="gg-->at"
     misc_feature    2933..2934
                     /label=cc-->at
                     /note="cc-->at"
     misc_feature    2958..2961
                     /label=tata
                     /note="tata"
     misc_feature    2968..2971
                     /label=tata
                     /note="tata"
     misc_feature    2981
                     /label=c-->a, removal Esp3I site
                     /note="c-->a, removal Esp3I site"
     misc_feature    3002..3006
                     /label=alcR-binding-site
                     /note="alcR-binding-site"
     misc_feature    3015..3019
                     /label=alcR-binding-site
                     /note="alcR-binding-site"
     misc_feature    3040..3041
                     /label=gg-->at
                     /note="gg-->at"
     misc_feature    3062..3063
                     /label=cc-->ta
                     /note="cc-->ta"
     misc_feature    3076..3079
                     /label=cccc-->tata
                     /note="cccc-->tata"
     misc_feature    3080..3082
                     /label=alcR-binding-site
                     /note="alcR-binding-site"
     misc_feature    3095..3100
                     /label=tatata
                     /note="tatata"
     misc_feature    3143..3146
                     /label=caat
                     /note="caat"
     misc_feature    3169..3172
                     /label=AATG
                     /note="AATG"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
      361 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
      421 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
      481 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
      541 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
      601 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
      661 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
      721 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
      781 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
      841 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
      901 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
      961 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     1021 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     1081 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     1141 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga
     1201 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat
     1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2161 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact
     2221 ctgtggtctc aggagacgtc gctctccccg atgacataca ggaggggtca gggcattcga
     2281 gaaataccgt ggatacagtt gggcatttct agggctgaat gggaaggaga gagttttgaa
     2341 ataggcgttc cgttctgctt agggtatttg ggaacaatca atgttcaatg tacatttaat
     2401 ccacgatttt ataaaacgtc atcctttgcc ctcccttctt atttgccaat accaaaaatc
     2461 ttactgcagt ggttcggtaa tcgcagagtt aaatctgggc tcggtggcag atctgcgatg
     2521 ctccataacc gttcagatgt tgattggaac tgggtgcggt agacagctcg cagaccgagt
     2581 gaacgatgca tgcggaaccg cacgagggcg tatacctaag acactttgag atgcatgcgg
     2641 aaccgcacga gggccacggc cggaacactg taagtccctg atgcatgcgg aaccgcacga
     2701 gggctcgtat ttcttcgcct gtgtgatgca tgcggaaccg cacgagggcg tagctaccat
     2761 ccaataacct acaggatgca tgcggaaccg cacgagggcc tgaaaagctg attgtgatag
     2821 ttcccacttg tccgtccgca tcggcatccg cagctcggga tagttccgac ctaggattgg
     2881 atgcatgcgg aaccgcacga gggcggatcg gaaattgaca caccactcct ctatacgcac
     2941 cgttcaagag gtacgcgtat agagccgtat agagcagaga aggagcactt tctggtactg
     3001 tccgcacggg atgtccgcac ggagagccac aaacgagcga tgccccgtac gtgctctcct
     3061 ataccaggat cgcattatag catagctgaa catctatata aagaccccca aggttctcag
     3121 tctcaccaac atcatcaacc aacaatcaac agttctctac tcagttaaaa tgtgagacca
     3181 cgaa
//
