LOCUS       Exported File           3731 bp ds-DNA    circular SYN 11-MAY-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pFTK035 CP0_30_Pc_ergA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 11, 2021 from SnapGene 1.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     CDS             215..1225
                     /codon_start=1
                     /note="Sm/Sp no DraIII"
                     /note="color: #ff9966"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     variation       1192
                     /note="G to A"
                     /note="color: #3366cc"
     misc_feature    1385..1999
                     /note="rep (pMB1)"
                     /note="color: #66cc99; direction: RIGHT"
     rep_origin      1984
                     /note="ORI"
                     /note="color: #3366cc"
     CDS             2236..3715
                     /codon_start=1
                     /note="ergA"
                     /note="color: #993366"
                     /translation="MTLLNGHASLSPAAQRRIEHHEADIVIVGAGVLGCALAVALGNQG
                     RSVILLEKSLEEPNRIVGELLQPGGVQALEQLGLRDCLEDIDGINVKGYWVTYFGEPVL
                     LEYPKSSPTSPTPLGRAFHHGRFVMKLRAAALSCPNVTVVETKVTGLITSSYTQEVLGV
                     ECITKDVKDCYFGQLTVAADGYNSVFRKEHHQYTPKRRSKFHGLELIDAKLPAPNTGHV
                     LLSDNPPVLMYQIGTHETRILIDIPDNLPAASVKNGGVKGYMRNNILPKLPEGVQQSFS
                     DALEKGQLRSMPNSFLPASANKTPGLMILGDALNMRHPLTGGGMTVALNDVCVIRELLS
                     PERVPNLSNTGLVLEQLAEFHWKRKNSSSVINILAQALYALFAADSTHAHSSG*FAITN
                     CSFNRLLPQSSSAWLFPLLPDWTSWRPCRVVSRSNQETPRPCQPLLLRCLPRHLGPHMR
                     YPSVQAIPSPILRRHDIVHSLCRDFAIHLDRDLVL"
     misc_feature    2952
                     /note="G-->A for BsaI-site removal"
                     /note="color: #a6acb3"
     misc_feature    3392..3446
                     /note="intron1"
                     /note="color: #a6acb3"
     misc_feature    3716..3719
                     /note="GCTT"
                     /note="color: #3366cc"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
      361 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
      421 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
      481 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
      541 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
      601 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
      661 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
      721 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
      781 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
      841 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
      901 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
      961 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     1021 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     1081 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     1141 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga
     1201 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat
     1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2161 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact
     2221 ctgtggtctc aaatgatgac cttgctcaat ggccacgcct ccctctcccc ggccgctcaa
     2281 cgccgcatcg aacaccatga agctgatatt gtaattgtgg gtgccggagt cctcggctgt
     2341 gcacttgcag ttgcactggg aaatcaaggg cgcagtgtaa tcctgctgga gaagtcgttg
     2401 gaagagccca accgtattgt tggtgaacta ctccaaccgg gaggtgttca ggcgctcgag
     2461 caactagggc tacgggattg tctggaagat atcgatggca tcaatgtgaa gggatactgg
     2521 gttacttact ttggggagcc ggttctcctc gaatacccca agtccagccc aacctcgcct
     2581 acaccgttgg gacgagcctt ccaccatggc cgattcgtga tgaaactacg ggcagccgct
     2641 ctgtcgtgcc ccaatgtcac ggtggtcgaa accaaggtta ccggcctcat cacatcctca
     2701 tatactcagg aagtccttgg agtcgagtgt ataaccaagg atgtcaagga ctgctacttc
     2761 ggtcaactca ccgtggccgc cgacggttat aactccgttt tccgcaagga acaccaccag
     2821 tatacaccca aacggcgctc aaagttccat ggcttggagc tcatcgatgc taaactgccc
     2881 gctcccaaca ctggtcatgt gcttctcagc gataatccac ccgtacttat gtaccagatc
     2941 ggcacccatg aaacccgaat ccttatcgat atccctgata acctgcctgc ggcctcggtc
     3001 aagaacggtg gtgtcaaggg ttacatgcga aacaacatcc tgcccaagct cccagagggt
     3061 gtccagcagt cgttctccga tgccctagag aagggccagc tacggtcaat gccgaactcg
     3121 ttcctgcctg cctcggccaa caagacaccc ggcttgatga ttctgggcga tgcgcttaac
     3181 atgcggcacc ctctcacagg tggaggcatg actgtggcgc tcaacgatgt ttgtgtcatc
     3241 cgcgaattgc tcagcccaga gcgcgtgccc aacctctcca atacaggcct tgttctcgag
     3301 cagctcgcag agttccactg gaaacgaaag aactcatctt cggtcatcaa tatcctcgca
     3361 caggctctct atgcgctctt cgctgcagac agtacgcacg ctcactcttc aggttaattt
     3421 gcaataacta actgttcatt caacagacta ttacctcaaa gctcttcagc gtggctgttt
     3481 ccgctacttc cagattggac cagttggcgg ccctgtcggg ttgttagccg gtctaatcaa
     3541 gaaacccctc gtccttgtca gccacttctt ctccgttgcc ttcctcgcca tctgggtcca
     3601 catatgcgat acccctctgt ccaagctatt cctagcccca tactacgccg tcatgatatt
     3661 gtacacagcc tctgtcgtga ttttgccata catctggacc gagatctggt actgagcttt
     3721 gagaccacga a
//
