LOCUS       Exported                2986 bp ds-DNA     circular SYN 15-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pFTK042 MC_0_82_Anid-hisB
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 6, 2021 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     CDS             215..1225
                     /codon_start=1
                     /label=Sm/Sp no DraIII
                     /label=Sm/Sp\no\DraIII
                     /note="/vntifkey=4"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     variation       1192
                     /label=G to A
                     /label=G\to\A
                     /label=nonstandard type: mutation
                     /note="/vntifkey=62"
     misc_feature    1385..1999
                     /label=rep (pMB1)
                     /label=rep\(pMB1)
                     /note="/vntifkey=21"
     rep_origin      1984
                     /label=ORI
                     /note="RNaseH cleavage point"
                     /note="/vntifkey=33"
     CDS             2236..2970
                     /codon_start=1
                     /locus_tag="A nidulans his3"
                     /label=A nidulans his3
                     /translation="MPLPARTASLSRNTNETKIQVSLSLDGGVLPPYEPSSHFPVPTDP
                     LEAEAAKKGIVPNKDAAHATQFTPTQQITISTGIGFLDHMLHALAKHSGWSLAIRAKGD
                     LYIDDHHTTEDTFLALGTAFTTALGARQSLARFGRGDAPLDEALSWAVIDLSSRPWAVI
                     NIGFKREKIGDLSTEMITHGLQSFAQAAGVTLHIGCTYGDNDHHRAESAFKALAVAIRA
                     ACARRVEGEVGAGDVVSTKGVL"
     primer_bind     2236..2253
                     /locus_tag="818_Anhis_newfw"
                     /label=818_Anhis_newfw
     primer_bind     2269..2288
                     /locus_tag="826_hisB_split_fw"
                     /label=826_hisB_split_fw
     misc_feature    2283
                     /label=G--> A for removal of BsaI-site
     misc_feature    2298
                     /label=C-->T for removal of BsaI-Site
     misc_feature    2577
                     /label=A --> G for removal of BpiI-Site
     primer_bind     complement(2913..2930)
                     /locus_tag="827_hisB_split_rev"
                     /label=827_hisB_split_rev
     primer_bind     complement(2951..2970)
                     /locus_tag="819_anhis_newrev"
                     /label=819_anhis_newrev
     misc_feature    2971..2974
                     /label=GCTT
                     /note="/vntifkey=21"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
      361 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
      421 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
      481 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
      541 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
      601 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
      661 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
      721 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
      781 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
      841 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
      901 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
      961 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     1021 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     1081 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     1141 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga
     1201 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat
     1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2161 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact
     2221 ctgtggtctc aaatgatgcc ccttcctgcc cgcacagcat cgctctcgcg aaacaccaac
     2281 gaaaccaaaa tccaggtttc tctctctcta gatggcggcg ttctccctcc ctacgaaccc
     2341 tcctctcact ttcccgtacc taccgaccct cttgaagccg aagctgccaa gaagggcatc
     2401 gtcccaaata aagatgccgc acacgcaacg cagttcacgc cgacgcaaca aatcactatc
     2461 agcacgggaa ttgggttcct agaccatatg ctgcacgcac ttgcgaagca ctcggggtgg
     2521 agcttggcca tcagggccaa gggagactta tacatcgacg atcaccacac aactgaggac
     2581 acttttcttg ctcttggtac cgcatttact accgccctag gagcccggca atccctcgcg
     2641 cgattcggcc gtggcgatgc ccctctggac gaggctctct cgtgggcagt cattgatctc
     2701 tctagccggc catgggcagt catcaacatt ggcttcaaac gcgaaaagat cggcgacctc
     2761 agcacggaga tgattacgca cggtctgcag agctttgcgc aggcggctgg tgttaccctt
     2821 cacattggct gcacctacgg cgacaatgac catcaccggg cggagagcgc gttcaaggca
     2881 ctggctgttg ctattagggc ggcttgtgcg cggagggttg agggcgaagt cggtgccggt
     2941 gatgtggtca gcaccaaagg tgtgctctaa gctttgagac cacgaa
//
