LOCUS       Exported                5007 bp ds-DNA     circular SYN 07-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pFTK054 YN0_56_QS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 7, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             215..1225
                     /codon_start=1
                     /label=Sm/Sp no DraIII
                     /note="Sm/Sp no DraIII"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     variation       1192
                     /label=G to A
                     /note="G to A"
     misc_feature    1385..1999
                     /label=rep
                     /note="rep"
     rep_origin      1984
                     /label=ORI
                     /note="ORI"
     CDS             2233..4989
                     /codon_start=1
                     /label=QS codon-optimized
                     /note="QS codon-optimized"
                     /translation="MNTIPARHVGDVAARDPLPLPHISSSVASGMKRSFATMAMLYNDT
                     GNSNDVGAHARRPPRTLSNSRSTSAHRVPLGSWSAPNSPPRRALPHHPITASFDPDASI
                     VIAGIRGAGKSTLAIMASTAMKRKIVDLESEFHHLTGLSSSSYKKTHGPVDYGRRQIAI
                     LQNILNLHRTRAILVCSWLERDVQAMLQDFSVSNPVIYVLRDAKAIEAHLKGYDKSKVG
                     TLLDATSTVLRRCTRFEFFNVSEENLDTHSASTSPPAVPDQRHTAPYLTLKRAERHFLK
                     FLSLILPKGTIPFVESAFPLASVPVEQRRFTYALALPVSALLDKGVDIQELDVGVDAIE
                     IIVDDLATSESGPTSPLGLAPHRASEISRVVGEIRRDTVIPIILHVVFPERALYEEALL
                     ALYMTYLNHALRLAPDYLTVDLGLDSGLLGQLTTVQGTTKVIGNKQLAEVNSPRWGDPS
                     WLQAYEKAQNTGCDLVRLTRPASNPRDNTDIRQFHVAVEAVGGPRLPFIAYNTGRLGRT
                     SMCFNEILTPVTPVPTKEDAIGLRNPAHRYLQPPLTALEATQALYSAFVHDPMKLYVFG
                     ANVGYSLSPAMHNAALKACGIPHHYKPLSTANIGTLREVISDPQFAGASVGLPFKVEII
                     SLTHSLSRHAKAIGAVNTLIPVRHLTADGGIPDEVSMFNNISQAGAVRALYGENTDWIG
                     IRACLRRGLSPANAVRSTSTGLVIGAGGMARAAVYAMLQLGVKKILIFNRTFANAEKLV
                     LHFENLLVRDALPLLSTGPRSHDNTCFHIIRSRDDPLPENFKNPTMIVSCIPTHTVDNT
                     PDPEFTVPLHWLDNPTGGIVLELDYKCLTSPLLEQTRREAHRGWVAMDGLDLLPEQGFA
                     QFELFTGRRAPRRLMRREVLRAYPDDQAKSHTAQLQPRLNGIATQIS"
     misc_feature    2443..2447
                     /label=DraIII
                     /note="DraIII"
     misc_feature    3391..3392
                     /label=BpiI
                     /note="BpiI"
     misc_feature    3394..3396
                     /label=BpiI
                     /note="BpiI"
     misc_feature    3847..3848
                     /label=BpiI
                     /note="BpiI"
     misc_feature    3850..3853
                     /label=fusion
                     /note="fusion"
     misc_feature    3851..3852
                     /label=BpiI
                     /note="BpiI"
     misc_feature    4992..4995
                     /label=GCTT
                     /note="GCTT"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
      361 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
      421 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
      481 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
      541 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
      601 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
      661 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
      721 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
      781 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
      841 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
      901 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
      961 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     1021 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     1081 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     1141 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga
     1201 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat
     1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2161 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact
     2221 ctgtggtctc aaatgaacac tattccagcg cgtcacgttg gagatgtggc agctcgtgac
     2281 ccactccccc tgccacatat aagttccagt gtggccagtg gcatgaaacg atcgtttgcc
     2341 actatggcca tgctctataa tgatacgggc aatagcaatg atgtcggagc gcatgcgcga
     2401 cgcccaccgc gcactctgag taacagccgc agcacatcgg ctcaccgggt cccattgggt
     2461 agctggagcg ccccgaactc gccaccgcga cgcgcactcc cacaccaccc cataacagct
     2521 tcgttcgacc cggacgcctc gatcgtgatt gccggaatcc gcggtgctgg aaagtccact
     2581 ctcgccataa tggcttcgac ggcgatgaag cgcaagatcg ttgacctcga atcggaattt
     2641 caccacctga ctggcctgtc ctccagcagc tacaagaaaa cacacggccc ggtggactat
     2701 ggtcgccgcc agatagcaat cctgcagaac atccttaacc tgcaccgaac gcgcgccatt
     2761 ctggtatgtt cctggttgga gcgcgatgtg caggcaatgc ttcaggattt ttcggtcagc
     2821 aacccggtga tctatgtcct cagggatgcc aaagccattg aggcccacct gaaaggttat
     2881 gacaagtcga aggtgggtac gttgctggat gcgacctcga ccgtcctgag gcgatgcacg
     2941 cgatttgagt tcttcaatgt ctccgaagaa aatctggata ctcattccgc cagcacgtcc
     3001 cccccagcgg ttccagatca gaggcatact gctccctacc tgactcttaa gcgcgcagag
     3061 cgtcactttc ttaagttcct gtccttgatt ttgcccaaag gaaccatccc gttcgtagaa
     3121 tccgccttcc cactggcgtc ggttccggtg gagcaacgcc gcttcaccta tgcgctggcg
     3181 cttcccgtgt cggcactcct ggacaaggga gtcgacattc aggagcttga cgtaggcgtc
     3241 gacgcaatcg aaattatcgt agacgatctg gccacaagcg aatccggccc aactagcccc
     3301 ctcggcctcg ccccgcaccg agcaagcgaa atctccaggg tggttggcga gatccgcagg
     3361 gataccgtca tacccatcat cctccacgtg gttttcccgg agagggccct gtacgaagag
     3421 gccctgttgg ccctgtacat gacctacctg aaccacgcct tgcgtctcgc gcccgattac
     3481 cttacagtgg acctcggact ggatagcggc ctgctgggtc agttgacgac agtgcagggc
     3541 acaactaaag tgattggtaa caagcagctg gctgaagtga atagccctcg ctggggcgac
     3601 ccatcgtggt tgcaggccta cgaaaaggcc caaaatactg gctgtgacct tgtgcggttg
     3661 acacgacccg cctcgaaccc gcgtgacaat actgacatcc gtcagttcca tgttgccgtg
     3721 gaggctgtgg gtggcccacg actgcccttc atcgcctata atactggtag gctcggccga
     3781 accagtatgt gcttcaacga aatcctgacg ccagtcactc ccgtccccac caaggaggat
     3841 gccattggtc tgcgcaatcc ggcacaccgt tacctgcagc caccgttgac tgcactggaa
     3901 gcaacccagg ccctgtacag cgcgtttgtt cacgacccaa tgaagctgta cgttttcgga
     3961 gctaatgttg gatacagcct gagccccgct atgcacaatg ccgcgttgaa agcctgcgga
     4021 atcccgcatc attacaagcc cctgagtact gctaacatag gcacccttcg tgaggtgatc
     4081 tccgacccgc agttcgcggg agctagtgta ggtctgccct ttaaggtgga aatcattagc
     4141 ctgactcact cgctgagccg tcacgctaaa gccattggcg cggtgaatac gttgattcct
     4201 gtgaggcatc ttacagcaga tggaggcatc cccgacgagg tgtccatgtt taataacatc
     4261 agccaggcag gtgctgtgag ggcattgtac ggcgagaata ccgattggat cggaatccgt
     4321 gcctgtctgc gtcgaggttt gagtcctgca aatgcagtcc gctccacatc cactggattg
     4381 gtgatcggag caggcggcat ggcccgagca gcggtgtacg caatgctcca gctcggagtc
     4441 aagaagatcc tcattttcaa ccgcaccttc gctaacgccg agaagctcgt gctgcatttc
     4501 gagaatctgt tggtcaggga tgccctgccc ctgttgtcga caggccctcg cagccacgat
     4561 aacacgtgtt ttcacatcat acggtcgagg gatgatccgc tcccagagaa cttcaagaac
     4621 cctaccatga tagtgtcgtg catcccaacg cacactgtag ataatacgcc cgatcccgag
     4681 ttcaccgtgc cattgcactg gttggacaac ccgaccggcg gaatcgtact ggagctggac
     4741 tacaagtgct tgacaagtcc actgttggag cagacgcggc gtgaggctca tcgcggatgg
     4801 gttgcgatgg atggcttgga cctgctccct gaacaaggat ttgcgcagtt tgaattgttc
     4861 accggccgac gtgcaccgcg tcgcctgatg cgccgcgagg tcctgcgagc atatccggac
     4921 gaccaggcca agagtcatac tgcacagctg caacctcgtc tcaatggaat agcaacgcag
     4981 atttcgtaaa agctttgaga ccacgaa
//
