LOCUS       Exported                6352 bp ds-DNA     circular SYN 07-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pFTK063 LM0_34 dSpCas9m4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Laszlo
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 7, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     CDS             215..1225
                     /codon_start=1
                     /label=Sm/Sp no DraIII
                     /note="Sm/Sp no DraIII"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     variation       1192
                     /label=G to A
                     /note="G to A"
     misc_feature    1385..1999
                     /label=rep
                     /note="rep"
     rep_origin      1984
                     /label=ORI
                     /note="ORI"
     CDS             2233..6336
                     /codon_start=1
                     /label=dCas9m4
                     /note="dCas9m4"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    4748..4749
                     /label=AC to CT
                     /note="AC to CT"
     misc_feature    4818..4821
                     /label=GGCC
                     /note="GGCC"
     misc_feature    4819..4820
                     /label=AC to GC
                     /note="AC to GC"
     misc_feature    6337..6340
                     /label=AGGT
                     /note="AGGT"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
      361 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
      421 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
      481 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
      541 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
      601 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
      661 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
      721 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
      781 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
      841 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
      901 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
      961 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     1021 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     1081 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     1141 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga
     1201 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat
     1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2161 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact
     2221 ctgtggtctc aaatggacaa gaagtattct atcggactgg ccatcgggac taatagcgtc
     2281 gggtgggccg tgatcactga cgagtacaag gtgccctcta agaagttcaa ggtgctcggg
     2341 aacaccgacc ggcattccat caagaaaaat ctgatcggag ctctcctctt tgattcaggg
     2401 gaaaccgctg aagcaacccg cctcaagcgg actgctagac ggcggtacac caggaggaag
     2461 aaccggattt gttaccttca agagatattc tccaacgaaa tggcaaaggt cgacgacagc
     2521 ttcttccata ggctggaaga atcattcctc gtggaagagg ataagaagca tgaacggcat
     2581 cccatcttcg gtaatatcgt cgacgaggtg gcctatcacg agaaataccc aaccatctac
     2641 catcttcgca aaaagctggt ggactcaacc gacaaggcag acctccggct tatctacctg
     2701 gccctggccc acatgatcaa gttcagaggc cacttcctga tcgagggcga cctcaatcct
     2761 gacaatagcg atgtggataa actgttcatc cagctggtgc agacttacaa ccagctcttt
     2821 gaagagaacc ccatcaatgc aagcggagtc gatgccaagg ccattctgtc agcccggctg
     2881 tcaaagagcc gcagacttga gaatcttatc gctcagctgc cgggtgaaaa gaaaaatgga
     2941 ctgttcggga acctgattgc tctttcactt gggctgactc ccaatttcaa gtctaatttc
     3001 gacctggcag aggatgccaa gctgcaactg tccaaggaca cctatgatga cgatctcgac
     3061 aacctcctgg cccagatcgg tgaccaatac gccgaccttt tccttgctgc taagaatctt
     3121 tctgacgcca tcctgctgtc tgacattctc cgcgtgaaca ctgaaatcac caaggcccct
     3181 ctttcagctt caatgattaa gcggtatgat gagcaccacc aggacctgac cctgcttaag
     3241 gcactcgtcc ggcagcagct tccggagaag tacaaggaaa tcttctttga ccagtcaaag
     3301 aatggatacg ccggctacat cgacggaggt gcctcccaag aggaatttta taagtttatc
     3361 aaacctatcc ttgagaagat ggacggcacc gaagagctcc tcgtgaaact gaatcgggag
     3421 gatctgctgc ggaagcagcg cactttcgac aatgggagca ttccccacca gatccatctt
     3481 ggggagcttc acgccatcct tcggcgccaa gaggacttct acccctttct taaggacaac
     3541 agggagaaga ttgagaaaat tctcactttc cgcatcccct actacgtggg acccctcgcc
     3601 agaggaaata gccggtttgc ttggatgacc agaaagtcag aagaaactat cactccctgg
     3661 aacttcgaag aggtggtgga caagggagcc agcgctcagt cattcatcga acggatgact
     3721 aacttcgata agaacctccc caatgagaag gtcctgccga aacattccct gctctacgag
     3781 tactttaccg tgtacaacga gctgaccaag gtgaaatatg tcaccgaagg gatgaggaag
     3841 cccgcattcc tgtcaggcga acaaaagaag gcaattgtgg accttctgtt caagaccaat
     3901 agaaaggtga ccgtgaagca gctgaaggag gactatttca agaaaattga atgcttcgac
     3961 tctgtggaga ttagcggggt cgaagatcgg ttcaacgcaa gcctgggtac ttaccatgat
     4021 ctgcttaaga tcatcaagga caaggatttt ctggacaatg aggagaacga ggacatcctt
     4081 gaggacattg tcctgactct cactctgttc gaggaccggg aaatgatcga ggagaggctt
     4141 aagacctacg cccatctgtt cgacgataaa gtgatgaagc aacttaaacg gagaagatat
     4201 accggatggg gacgccttag ccgcaaactc atcaacggaa tccgggacaa acagagcgga
     4261 aagaccattc ttgatttcct taagagcgac ggattcgcta atcgcaactt catgcaactt
     4321 atccatgatg attccctgac ctttaaggag gacatccaga aggcccaagt gtctggacaa
     4381 ggtgactcac tgcacgagca tatcgcaaat ctggctggtt cacccgctat taagaagggt
     4441 attctccaga ccgtgaaagt cgtggacgag ctggtcaagg tgatgggtcg ccataaacca
     4501 gagaacattg tcatcgagat ggccagggaa aaccagacta cccagaaggg acagaagaac
     4561 agcagggagc ggatgaaaag aattgaggaa gggattaagg agctcgggtc acagatcctt
     4621 aaagagcacc cggtggaaaa cacccagctt cagaatgaga agctctatct gtactacctt
     4681 caaaatggac gcgatatgta tgtggaccaa gagcttgata tcaacaggct ctcagactac
     4741 gacgtggctg ccatcgtccc tcagagcttc ctcaaagacg actcaattga caataaggtg
     4801 ctgactcgct cagacaaggc ccggggaaag tcagataacg tgccctcaga ggaagtcgtg
     4861 aaaaagatga agaactattg gcgccagctt ctgaacgcaa agctgatcac tcagcggaag
     4921 ttcgacaatc tcactaaggc tgagaggggc ggactgagcg aactggacaa agcaggattc
     4981 attaaacggc aacttgtgga gactcggcag attactaaac atgtcgccca aatccttgac
     5041 tcacgcatga ataccaagta cgacgaaaac gacaaactta tccgcgaggt gaaggtgatt
     5101 accctgaagt ccaagctggt cagcgatttc agaaaggact ttcaattcta caaagtgcgg
     5161 gagatcaata actatcatca tgctcatgac gcatatctga atgccgtggt gggaaccgcc
     5221 ctgatcaaga agtacccaaa gctggaaagc gagttcgtgt acggagacta caaggtctac
     5281 gacgtgcgca agatgattgc caaatctgag caggagatcg gaaaggccac cgcaaagtac
     5341 ttcttctaca gcaacatcat gaatttcttc aagaccgaaa tcacccttgc aaacggtgag
     5401 atccggaaga ggccgctcat cgagactaat ggggagactg gcgaaatcgt gtgggacaag
     5461 ggcagagatt tcgctaccgt gcgcaaagtg ctttctatgc ctcaagtgaa catcgtgaag
     5521 aaaaccgagg tgcaaaccgg aggcttttct aaggaatcaa tcctccccaa gcgcaactcc
     5581 gacaagctca ttgcaaggaa gaaggattgg gaccctaaga agtacggcgg attcgattca
     5641 ccaactgtgg cttattctgt cctggtcgtg gctaaggtgg aaaaaggaaa gtctaagaag
     5701 ctcaagagcg tgaaggaact gctgggtatc accattatgg agcgcagctc cttcgagaag
     5761 aacccaattg actttctcga agccaaaggt tacaaggaag tcaagaagga ccttatcatc
     5821 aagctcccaa agtatagcct gttcgaactg gagaatgggc ggaagcggat gctcgcctcc
     5881 gctggcgaac ttcagaaggg taatgagctg gctctcccct ccaagtacgt gaatttcctc
     5941 taccttgcaa gccattacga gaagctgaag gggagccccg aggacaacga gcaaaagcaa
     6001 ctgtttgtgg agcagcataa gcattatctg gacgagatca ttgagcagat ttccgagttt
     6061 tctaaacgcg tcattctcgc tgatgccaac ctcgataaag tccttagcgc atacaataag
     6121 cacagagaca aaccaattcg ggagcaggct gagaatatca tccacctgtt caccctcacc
     6181 aatcttggtg cccctgccgc attcaagtac ttcgacacca ccatcgaccg gaaacgctat
     6241 acctccacca aagaagtgct ggacgccacc ctcatccacc agagcatcac cggactttac
     6301 gaaactcgga ttgacctctc acagctcgga ggggataggt tgagaccacg aa
//
