LOCUS       Exported                3970 bp ds-DNA     circular SYN 25-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pFTK087 MC_0_26_pANtRNA-Pro1-sgRNA TU
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 6, 2021 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        110..214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             434..1225
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1396..1984
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      1984
                     /label=ORI
                     /note="RNaseH cleavage point"
                     /note="/vntifkey=33"
     promoter        2236..2860
                     /label=ptRNA-Pro-1 promoter
     tRNA            2861..2951
                     /gene="tRNA-Pro (AGG)"
                     /locus_tag="An01e12280"
                     /product="tRNA-Pro An01e14470"
                     /label=tRNA -Pro-1 (AGG) - An01e14470
                     /inference="profile:same species:1.4"
     gene            2861..2951
                     /gene="tRNA-Pro (AGG)"
                     /locus_tag="An01e12280"
     misc_feature    2952..2958
                     /label=Esp3I
     promoter        2993..3116
                     /label=P lac prom
                     /label=P\lac\prom
                     /note="/vntifkey=30"
     protein_bind    3013..3034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3049..3079
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        3085
                     /label=P(LAC)
                     /note="lac promoter"
                     /note="/vntifkey=30"
     protein_bind    3087..3103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3111..3127
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3123..3446
                     /codon_start=1
                     /label=ALPHA
                     /note="/vntifkey=4"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    3140..3196
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(3197..3213)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3532..3538
                     /label=Esp3I
     misc_RNA        3539..3614
                     /label=g RNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    3630..3954
                     /label=TtrpC
     misc_feature    3955..3958
                     /label=CGCT
                     /note="/vntifkey=21"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
      361 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
      421 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
      481 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
      541 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
      601 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
      661 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
      721 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
      781 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
      841 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
      901 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
      961 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     1021 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     1081 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     1141 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga
     1201 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat
     1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2161 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact
     2221 ctgtggtctc aggagtatgc atgcaatgta gttgtagggc tctggcactc tgtaactcta
     2281 ggagtgatga acttagttac tcaagatggg atatatataa tcggttcttt ttggtcgggg
     2341 tatgttgctg agatacaaca ccggggggta ggtgcaggta gtcgctcagg tatgtaagta
     2401 gaatcgcgcg agggtgttgt tgcagttttc tggtcattgg gctttggggg aggcgccgcg
     2461 ttcaacatcg atgagagggt gtattccatg gatgaagtgc cgggcatgat atttcgcggt
     2521 gagtgatgcc gttcgtaaaa gttgaaaggc gataagcagg caccatccct ccgaagtagt
     2581 atgaagtacc caccagtata tattcagcca acacgcctcc gactcacaac ttaaaggtta
     2641 gcaaccatca ctcccttgtt cattacttac cttactagta gtacttagaa gtctaagcca
     2701 atctaaattt caacaacgta tatagaaagg atattcttct tccgcggccg tataaccctg
     2761 tatgacaaac atcacgacag cacaggtagt tacggaactg cagctaaaat tgaaaaaaga
     2821 ctcatagagc ataaaagaaa accgtgtttc aacaggcaac gcccgggtgg tctagtggta
     2881 tgattctcgc ttagggatac aaacccaagc atatctgcga gaggtcccgc gttcgatccg
     2941 cggctcgggc ctgagacgca gctggcacga caggtttccc gactggaaag cgggcagtga
     3001 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     3061 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     3121 ctatgaccat gattacgcca agcttgcatg cctgcaggtc gactctagag gatccccggg
     3181 taccgagctc gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     3241 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     3301 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
     3361 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     3421 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     3481 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gcgtctcagt
     3541 ttcagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
     3601 caccgagtcg gtgcgtttct ttttcttttt tagtagatgc cgaccgcggg atccacttaa
     3661 cgttactgaa atcatcaaac agcttgacga atctggatat aagatcgttg gtgtcgatgt
     3721 cagctccgga gttgagacaa atggtgttca ggatctcgat aagatacgtt catttgtcca
     3781 agcagcaaag agtgccttct agtgatttaa tagctccatg tcaacaagaa taaaacgcgt
     3841 tttcgggttt acctcttcca gatacagctc atctgcaatg cattaatgca ttgactgcaa
     3901 cctagtaacg ccttncaggc tccggcgaag agaagaatag cttagcagag ctatcgcttg
     3961 agaccacgaa
//
