LOCUS       Exported               10770 bp ds-DNA     circular SYN 07-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pFTK094 pLM-AMA002
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10770)
  AUTHORS   Laszlo Mozsik
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 7, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10770
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    2..65
                     /label=6-APA remaining
                     /note="6-APA remaining"
     promoter        complement(95..113)
                     /note="T7 promoter"
     primer_bind     complement(123..139)
                     /label=M13 fwd
                     /note="M13 fwd"
     rep_origin      281..736
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 ori"
     promoter        762..866
                     /note="AmpR promoter"
     misc_feature    1068..2247
                     /label=Cam
                     /note="Cam"
     rep_origin      2336..2924
                     /direction=RIGHT
                     /label=ori
                     /note="ori"
     protein_bind    3212..3233
                     /note="CAP binding site"
     promoter        3248..3278
                     /note="lac promoter"
     protein_bind    3286..3302
                     /note="lac operator"
     primer_bind     3310..3326
                     /label=M13 rev
                     /note="M13 rev"
     promoter        3347..3365
                     /note="T3 promoter"
     misc_feature    3399..4160
                     /label=PpcbC
                     /note="PpcbC"
     CDS             4161..4535
                     /codon_start=1
                     /label=BleoR
                     /note="BleoR"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      4620..4867
                     /note="CYC1 terminator"
     misc_feature    complement(4873..10122)
                     /label=AMA1
                     /note="AMA1"
     misc_feature    10170..10173
                     /label=GGAG
                     /note="GGAG"
     misc_feature    10175..10180
                     /label=BsaI
                     /note="BsaI"
     misc_feature    10181..10755
                     /label=LacZ / LVL0 reciever
                     /note="LacZ / LVL0 reciever"
     promoter        10215..10338
                     /note="P lac prom"
     promoter        10307
                     /note="P(LAC)"
     CDS             10345..10665
                     /codon_start=1
                     /label=ALPHA
                     /note="ALPHA"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    10755..10760
                     /label=BsaI
                     /note="BsaI"
     misc_feature    10762..10765
                     /label=CGCT
                     /note="CGCT"
ORIGIN
        1 gccgctcccc ccctgaaaga gttgatattg aaggtagtag gtacggtgca gggtaacgcg
       61 tgatggccgc caccgcggtg gagctccaat tcgccctata gtgagtcgta ttacgcgcgc
      121 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
      181 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
      241 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
      301 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
      361 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
      421 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
      481 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
      541 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
      601 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      661 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      721 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      781 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      841 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      901 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      961 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     1021 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttcgac
     1081 cgaataaata cctgtgacgg aagatcactt cgcagaataa ataaatcctg gtgtccctgt
     1141 tgataccggg aagccctggg ccaacttttg gcgaaaatga gacgttgatc ggcacgtaag
     1201 aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttgtc
     1261 gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt
     1321 tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg
     1381 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa
     1441 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc
     1501 ggaattacgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg
     1561 ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga
     1621 cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct
     1681 ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt
     1741 gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg ccccgttttc
     1801 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1861 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1921 tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca gttattggtg cccttaaacg
     1981 cctggttgct acgcctgaat aagtgataat aagcggatga atggcagaaa ttcgaaagca
     2041 aattcgaccc ggtcgtcggt tcagggcagg gtcgttaaat agccgcttat gtctattgct
     2101 ggtttaccgg tttattgact accggaagca gtgtgaccgt gtgcttctca aatgcctgag
     2161 gccagtttgc tcaggctctc cccgtggagg taataattga cgatatgatc ctttttttct
     2221 gatcaaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     2281 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     2341 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2401 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2461 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     2521 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2581 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2641 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2701 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2761 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2821 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2881 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2941 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3001 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3061 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     3121 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     3181 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     3241 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     3301 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     3361 aaagggaaca aaagctgggt accgggcccc ccctcgaggt cgactacatg tatctgcatg
     3421 ttgcatcggg aaatcccacc acagggacag ccaagcggcc ccgcgacttg gcagtgggca
     3481 aactacgccc gattctggtg ccaagaaccg agaagaatga gacagaccca cgttgcactc
     3541 taaccggatg ctatcgactt acggtggctg aagattcaac acgctgcaac gagagccaag
     3601 gtggtccgga cattttctac gtgccggttt accttggaac atcgccgtcg ttgagtgcac
     3661 gttgcctact ctctcgtggc ttggctgggc ccacgagccc gattgactcg acggtgttac
     3721 ttgggtatct atggccccgt tttctggcac ggtaatgata agtacttact agtcttcgag
     3781 cgagcggggg agtgttgctc tgcccgagca tcaacgattg gcctgatcgc accgtctgca
     3841 aatgccacgg tgcggaccga ctgaaatctc agaccaccaa agaccctccg acttcgagat
     3901 acggttacta attttacact ggctccagcg gccccatcca gtaagcatct gggctgcaag
     3961 cgtataatgt ctccaggttg tctcagcata aacaccccgc ccccgctcag gcacacagga
     4021 agagagctca ggtcgtttcc attgcgtcca tactcttcac tcattgtcat ctgcaggaga
     4081 acttcccctg tccctttgcc aagccctctc ttcgtcgttg tccacgcctt caagttttca
     4141 ccattatttt tctagacacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
     4201 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
     4261 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
     4321 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
     4381 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
     4441 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
     4501 actgcgtgca cttcgtggcc gaggagcagg actgaccgac gccgaccaac accgccggtc
     4561 cgacgcggcc cgacgggtcc gaggcctcgg agatccgtcc cccttttcct ttgtcgatat
     4621 catgtaatta gttatgtcac gcttacattc acgccctccc cccacatccg ctctaaccga
     4681 aaaggaagga gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt
     4741 agtattaaga acgttattta tatttcaaat ttttcttttt tttctgtaca gacgcgtgta
     4801 cgcatgtaac attatactga aaaccttgct tgagaaggtt ttgggacgct cgaaggcttt
     4861 aatttgcaag cttatttttt gtatactgtt ttgtgatagc acgaagtttt tccacggtat
     4921 cttgtaaaaa tatatatttg tggcgggctt acctacatca aattaataag agactaatta
     4981 taaactaaac acacaagcaa gctactttag ggtaaaagtt tataaatgct tttgacgtat
     5041 aaacgttgct tgtatttatt attacaatta aaggtggata gaaaacctag agactagtta
     5101 gaaactaatc tcaggtttgc gttaaactaa atcagagccc gagaggttaa cagaacctag
     5161 aaggggacta gatatccggg tagggaaaca aaaaaaaaaa acaagacagc cacatattag
     5221 ggagactagt tagaagctag ttccaggact aggaaaataa aagacaatga taccacagtc
     5281 tagttgacaa ctagatagat tctagattga ggccaaagtc tctgagatcc aggttagttg
     5341 caactaatac tagttagtat ctagtctcct ataactctga agctagaata acttactact
     5401 attatcctca ccactgttca gctgcgcaaa cggagtgatt gcaaggtgtt cagagactag
     5461 ttattgacta gtcagtgact agcaataact aacaaggtat taacctacca tgtctgccat
     5521 caccctgcac ttcctcgggc tcagcagcct tttcctcctc attttcatgc tcattttcct
     5581 tgtttaagac tgtgactagt caaagactag tccagaacca caaaggagaa atgtcttacc
     5641 actttcttca ttgcttgtct cttttgcatt atccatgtct gcaactagtt agagtctagt
     5701 tagtgactag tccgacgagg acttgcttgt ctccggattg ttggaggaac tctccagggc
     5761 ctcaagatcc acaacagagc cttctagaag actggtcaat aactagttgg tctttgtctg
     5821 agtctgactt acgaggttgc atactcgctc cctttgcctc gtcaatcgat gagaaaaagc
     5881 gccaaaactc gcaatatggc tttgaaccac acggtgctga gactagttag aatctagtcc
     5941 caaactagct tggatagctt acctttgccc tttgcgttgc gacaggtctt gcagggtatg
     6001 gttcctttct caccagctga tttagctgcc ttgctaccct cacggcggat ctgccataaa
     6061 gagtggctag aggttataaa ttagcactga tcctaggtac ggggctgaat gtaacttgcc
     6121 tttcctttct catcgcgcgg caagacaggc ttgctcaaat tcctaccagt cacaggggta
     6181 tgcacggcgt acggaccact tgaactagtc acagattagt tagcaactag tctgcattga
     6241 atggctgtac ttacgggccc tcgccattgt cctgatcatt tccagcttca ccctcgttgc
     6301 tgcaaagtag ttagtgacta gtcaaggact agttgaaatg ggagaagaaa ctcacgaatt
     6361 ctcgactccc ttagtattgt ggtccttgga cttggtgctg ctatatatta gctaatacac
     6421 tagttagact cacagaaact tacgcagctc gcttgcgctt cttggtagga gtcggggttg
     6481 ggagaacagt gccttcaaac aagccttcat accatgctac ttgactagtc agggactagt
     6541 caccaagtaa tctagatagg acttgccttt ggcctccatc agttccttca tagtgggagg
     6601 accattgtgc aatgtaaact ccatgccgtg ggagttcttg tccttcaagt gcttgaccaa
     6661 tatgtttctg ttggcagagg gaacctgtca actagttaat aactagtcag aaactatgat
     6721 agcagtagac tcactgtacg cttgaggcat cccttcactc ggcagtagac ttcatatgga
     6781 tggatatcag gcacgccatt gtcgtcctgt ggactagtca gtaactaggc ttaaagctag
     6841 tcgggtcggc ttactatctt gaaatccggc agcgtaagct ccccgtcctt aactgcctcg
     6901 agatagtgac agtactctgg ggactttcgg agatcgttat cgttatcgcg aatgctcggc
     6961 atactaactg ttgactagtc ttggactagt cccgagcaaa aaggattgga ggaggaggag
     7021 gaaggtgaga gtgagacaaa gagcgaaata agagcttcaa aggctatctc taagcagtat
     7081 gaaggttaag tatctagttc ttgactagat ttaaagagat ttcgactagt tatgtacctg
     7141 gagtttggat ataggaatgt gttgtggtaa cgaaatgtaa gggggaggaa agaaaaagtc
     7201 gtcaagaggt aactctaagt cggccattcc tttttgggag gcgctaacca taaacggcat
     7261 ggtcgactta gagttagctc agggaattta gggagttatc tgcgaccacc gaggaacggc
     7321 ggaatgccaa agaatcccga tggagctcta gctggcggtt gacaacccca ccttttggcg
     7381 tttctgcggc gttgcaggcg ggactggata cttcgtagaa ccagaaaggc aaggcagaac
     7441 gcgctcagca agagtgttgg aagtgatagc atgatgtgcc ttgttaacta ggtnccaatc
     7501 tgcagtatgc ttgatgttat ccaaagtgtg agagaggaag gtccaaacat acacgattgg
     7561 gagagggcct aggtataaga gtttttgagt agaacgcatg tgagcccagc catctcgagg
     7621 agattaaaca cgggccggca tttgatggct atgttagtac cccaatggaa acggtgagag
     7681 tccagtggtc gcagataact ccctaaattc cctgagctaa ctctaagtcg accatgccgt
     7741 ttatggttag cgcctcccaa aaaggaatgg ccgacttaga gttacctctt gacgactttt
     7801 tctttcctcc cccttacatt tcgttaccac aacacattcc tatatccaaa ctccaggtac
     7861 ataactagtc gaaatctctt taaatctagt caagaactag atacttaacc ttcatactgc
     7921 ttagagatag cctttgaagc tcttatttcg ctctttgtct cactctcacc ttcctcctcc
     7981 tcctccaatc ctttttgctc gggactagtc caagactagt caacagttag tatgccgagc
     8041 attcgcgata acgataacga tctccgaaag tccccagagt actgtcacta tctcgaggca
     8101 gttaaggacg gggagcttac gctgccggat ttcaagatag taagccgacc cgactagctt
     8161 taagcctagt tactgactag tccacaggac gacaatggcg tgcctgatat ccatccatat
     8221 gaagtctact gccgagtgaa gggatgcctc aagcgtacag tgagtctact gctatcatag
     8281 tttctgacta gttattaact agttgacagg ttccctctgc caacagaaac atattggtca
     8341 agcacttgaa ggacaagaac tcccacggca tggagtttac attgcacaat ggtcctccca
     8401 ctatgaagga actgatggag gccaaaggca agtcctatct agattacttg gtgactagtc
     8461 cctgactagt caagtagcat ggtatgaagg cttgtttgaa ggcactgttc tcccaacccc
     8521 gactcctacc aagaagcgca agcgagctgc gtaagtttct gtgagtctaa ctagtgtatt
     8581 agctaatata tagcagcacc aagtccaagg accacaatac taagggagtc gagaattcgt
     8641 gagtttcttc tcccatttca actagtcctt gactagtcac taactacttt gcagcaacga
     8701 gggtgaagct ggaaatgatc aggacaatgg cgagggcccg taagtacagc cattcaatgc
     8761 agactagttg ctaactaatc tgtgactagt tcaagtggtc cgtacgccgt gcatacccct
     8821 gtgactggta ggaatttgag caagcctgtc ttgccgcgcg atgagaaagg aaaggcaagt
     8881 tacattcagc cccgtaccta ggatcagtgc taatttataa cctctagcca ctctttatgg
     8941 cagatccgcc gtgagggtag caaggcagct aaatcagctg gtgagaaagg aaccataccc
     9001 tgcaagacct gtcgcaacgc aaagggcaaa ggtaagctat ccaagctagt ttgggactag
     9061 attctaacta gtctcagcac cgtgtggttc aaagccatat tgcgagtttt ggcgcttttt
     9121 ctcatcgatt gacgaggcaa agggagcgag tatgcaacct cgtaagtcag actcagacaa
     9181 agaccaacta gttattgacc agtcttctag aaggctctgt tgtggatctt gaggccctgg
     9241 agagttcctc caacaatccg gagacaagca agtcctcgtc ggactagtca ctaactagac
     9301 tctaactagt tgcagacatg gataatgcaa aagagacaag caatgaagaa agtggtaaga
     9361 catttctcct ttgtggttct ggactagtct ttgactagtc acagtcttaa acaaggaaaa
     9421 tgagcatgaa aatgaggagg aaaaggctgc tgagcccgag gaagtgcagg gtgatggcag
     9481 acatggtagg ttaatacctt gttagttatt gctagtcact gactagtcaa taactagtct
     9541 ctgaacacct tgcaatcact ccgtttgcgc agctgaacag tggtgaggat aatagtagta
     9601 agttattcta gcttcagagt tataggagac tagatactaa ctagtattag ttgcaactaa
     9661 cctggatctc agagactttg gcctcaatct agaatctatc tagttgtcaa ctagactgtg
     9721 gtatcattgt cttttatttt cctagtcctg gaactagctt ctaactagtc tccctaatat
     9781 gtggctgtct tgtttttttt ttttgtttcc ctacccggat atctagtccc cttctaggtt
     9841 ctgttaacct ctcgggctct gatttagttt aacgcaaacc tgagattagt ttctaactag
     9901 tctctaggtt ttctatccac ctttaattgt aataataaat acaagcaacg tttatacgtc
     9961 aaaagcattt ataaactttt accctaaagt agcttgcttg tgtgtttagt ttataattag
    10021 tctcttatta atttgatgta ggtaagcccg ccacaaatat atatttttaa caagataccg
    10081 tggaaaaact tcgtgctatc acaaaacagt atacaaaaaa taagctatcg aattcctgca
    10141 gccccagatc atcctgtctt cagtcttaag gagcgagacc cagctggcac gacaggtttc
    10201 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    10261 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    10321 aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca tgcctgcagg
    10381 tcgactctag aggatccccg ggtaccgagc tcgaattcac tggccgtcgt tttacaacgt
    10441 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
    10501 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
    10561 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
    10621 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aaaccagccc
    10681 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
    10741 tacagacaag ctgtggtctc ccgctttaag
//
