LOCUS       Exported                8421 bp ds-DNA     circular SYN 04-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8421)
  AUTHORS   Dell
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 10, 2021 from SnapGene Viewer 5.2.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8421
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             106..966
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1137..1725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1819..2059
                     /label=U6 Promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    2100..2135
                     /label=DR
     enhancer        2158..2537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2538..2741
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          2833..2965
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             2998..3018
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3019..5340
                     /codon_start=1
                     /label=dCas13X.1
                     /translation="AQVSKQTSKKRELSIDEYQGARKWCFTIAFNKALVNRDKNDGLFV
                     ESLLRHEKYSKHDWYDEDTRALIKCSTQAANAKAEALANYFSAYRHSPGCLTFTAEDEL
                     RTIMERAYERAIFECRRRETEVIIEFPSLFEGDRITTAGVVFFVSFFVERRVLDRLYGA
                     VSGLKKNEGQYKLTRKALSMYCLKDSRFTKAWDKRVLLFRDILAQLGRIPAEAYEYYHG
                     EQGDKKRANDNEGTNPKRHKDKFIEFALHYLEAQHSEICFGRRHIVREEAGAGDEHKKH
                     RTKGKVVVDFSKKDEDQSYYISKNNVIVRIDKNAGPRSYRMGLNELKYLVLLSLQGKGD
                     DAIAKLYRYRQHVENILDVVKVTDKDNHVFLPRFVLEQHGIGRKAFKQRIDGRVKHVRG
                     VWEKKKAATNEMTLHEKARDILQYVNENCTRSFNPGEYNRLLVCLVGKDVENFQAGLKR
                     LQLAERIDGRVYSIFAQTSTINEMHQVVCDQILNRLCRIGDQKLYDYVGLGKKDEIDYK
                     QKVAWFKEHISIRRGFLRKKFWYDSKKGFAKLVEEHLESGGGQRDVGLDKKYYHIDAIG
                     RFEGANPALYETLARDRLCLMMAQYFLGSVRKELGNKIVWSNDSIELPVEGSVGNEKSI
                     VFSVSDYGKLYVLDDAEFLGRICEYFMPHEKGKIRYHTVYEKGFRAYNDLQKKCVEAVL
                     AFEEKVVKAKKMSEKEGAHYIDFREILAQTMCKEAEKTAVNKVAAAFFAAHLKFVIDEF
                     GLFSDVMKKYGIEKEWKFPVK"
     CDS             5341..5361
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5362..5424
                     /codon_start=1
                     /label=linker
                     /translation="GGGGGSGGGGSGGGGSGGGGS"
     CDS             5425..6579
                     /codon_start=1
                     /label=REPAIRv2(hADAR2dd)
                     /translation="QLHLPQVLADAVSRLVLGKFGDLTDNFSSPHARRKVLAGVVMTTG
                     TDVKDAKVISVSTGGKCINGEYMSDRGLALNDCHAEIISRRSLLRFLYTQLELYLNNKD
                     DQKRSIFQKSERGGFRLKENVQFHLYISTSPCGDARIFSPHEPILEEPADRHPNRKARG
                     QLRTKIESGQGTIPVRSNASIQTWDGVLQGERLLTMSCSDKIARWNVVGIQGSLLSIFV
                     EPIYFSSIILGSLYHGDHLSRAMYQRISNIEDLPPLYTLNKPLLSGISNAEARQPGKAP
                     NFSVNWTVGDSAIEVINATTGKDELGRASRLCKHALYCRWMRVHGKVPSHLLRSKITKP
                     NVYHESKLAAKEYQAAKARLFTAFIKAGLGAWVEKPTEQDQFSLT"
     CDS             6580..6606
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    6640..6847
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        6916..7219
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7220..7423
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             7458..8168
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     DDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    8191..8415
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
       61 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      121 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      181 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      241 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      301 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      361 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      421 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      481 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
      541 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
      601 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
      661 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
      721 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
      781 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
      841 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
      901 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
      961 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1021 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1081 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1141 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1201 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1261 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1321 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1381 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1441 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1501 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1561 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1621 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1681 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1741 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1801 tcccctgatt ctctcgagga gggcctattt cccatgattc cttcatattt gcatatacga
     1861 tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta
     1921 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
     1981 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
     2041 tatatcttgt ggaaaggacg aaacaccggg gtcttcgata ttcaagcgtc ggaagacctg
     2101 ctggagcagc ccccgatttg tggggtgatt acagcttttt ttgtggacgg tgatatcggc
     2161 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     2221 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     2281 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     2341 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     2401 tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     2461 attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag
     2521 tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt
     2581 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     2641 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
     2701 gcggtaggcg tgtacggtgg gaggtctata taagcagagg tcgtttagtg aaccgtcaga
     2761 tcactagtag ctttattgcg gtagtttatc acagttaaat tgctaacgca gtcagtgctc
     2821 gactgatcac aggtaagtat caaggttaca agacaggttt aaggaggcca atagaaactg
     2881 ggcttgtcga gacagagaag attcttgcgt ttctgatagg cacctattgg tcttactgac
     2941 atccactttg cctttctctc cacaggggta ccgaagccgc tagtcgacgc caccatgccc
     3001 aagaagaagc ggaaggtggc ccaggtgagc aagcagacct ccaagaagag ggagctgagc
     3061 atcgacgagt accagggcgc ccggaagtgg tgcttcacca ttgccttcaa caaggccctg
     3121 gtgaaccggg acaagaacga cggcctgttc gtggaaagcc tgctgagaca cgagaagtac
     3181 agcaagcacg actggtacga cgaagatacc cgggccctga tcaagtgcag cacccaggcc
     3241 gccaacgcca aggctgaagc cctggcgaac tacttcagtg cttaccggca tagccctggc
     3301 tgcctgacct tcaccgccga ggacgaactg cggaccatca tggagagagc ctatgagcgg
     3361 gccatcttcg agtgcagaag aagagagaca gaggtgatca tcgagtttcc cagcctgttc
     3421 gagggcgacc ggatcaccac cgccggcgtg gtgtttttcg tgagcttttt cgtggaaaga
     3481 agagtgctgg atcggctgta tggagccgtg tccggcctga agaagaatga gggacagtac
     3541 aagctgaccc ggaaggccct gagcatgtac tgcctgaagg acagcagatt caccaaggcc
     3601 tgggataagc gggtgctgct gttcagagac atcctggccc agctgggaag aatccccgcc
     3661 gaggcctacg agtactacca cggcgagcag ggtgataaga agagagctaa cgacaatgag
     3721 ggcacaaatc ccaagcggca caaggacaag ttcatcgaat ttgcactgca ctacctggaa
     3781 gcccagcaca gcgagatctg cttcggcaga cgccacatcg tgcgggaaga ggccggcgcc
     3841 ggcgatgagc acaagaagca ccggaccaag ggaaaggtgg tggtggactt cagcaagaag
     3901 gacgaggacc agagctacta tatctccaag aacaacgtga tcgtgcggat cgacaagaac
     3961 gccggcccta gaagctaccg gatgggcctg aacgagctga agtacctcgt gctgctgagc
     4021 ctgcagggga agggcgacga tgccatcgcc aagctgtaca gatacagaca gcacgtggag
     4081 aacatcctgg atgtggtgaa ggtgaccgat aaggataacc acgtgttcct gccccgcttc
     4141 gtgctggagc agcacggcat cggcagaaag gccttcaagc agcggatcga tggacgggtg
     4201 aagcacgtgc ggggcgtgtg ggagaagaag aaggccgcca ccaatgaaat gaccctgcac
     4261 gagaaggcca gagacatcct gcagtacgtg aacgaaaact gcacccggtc cttcaaccct
     4321 ggcgaataca acagactgct ggtgtgcctg gtgggcaagg acgtggagaa ctttcaggcc
     4381 ggcctgaagc ggctgcagct ggccgaaagg atcgatggcc gggtgtactc catcttcgcc
     4441 cagaccagca ccatcaatga gatgcaccag gtggtgtgcg accagatcct gaaccggctg
     4501 tgcagaatcg gcgaccagaa gctgtacgat tacgtgggac tgggcaagaa ggacgaaatc
     4561 gactacaagc agaaggtggc ctggttcaag gagcacatca gcatccggag aggattcctg
     4621 agaaagaagt tctggtacga tagcaagaag ggattcgcaa agctggtgga ggaacacctg
     4681 gagtccggcg gcggccagcg cgacgtgggc ctggacaaga agtactacca catcgacgcc
     4741 atcggcagat tcgagggcgc caaccccgcc ctgtacgaga ccctggccag agatcggctg
     4801 tgcctcatga tggcccagta cttcctgggc agcgtgagaa aggaactggg caacaagatt
     4861 gtgtggagca acgacagcat cgaactgcct gtggaaggct ctgtgggaaa tgagaagagc
     4921 atcgtgttct ccgtgtctga ctacggcaag ctgtacgtgc tggacgatgc cgaattcctg
     4981 ggccggatct gcgaatactt catgccccac gaaaagggca agatccggta ccacacagtg
     5041 tacgaaaagg gctttagagc atacaacgac ctgcagaaga agtgcgtgga ggccgtgctg
     5101 gctttcgaag agaaggtggt gaaggccaag aagatgagcg agaaggaagg cgcccactac
     5161 atcgacttcc gggagatcct ggcccagacc atgtgcaagg aggccgagaa aaccgcagtg
     5221 aacaaggtgg cggctgcctt cttcgctgcg cacctgaagt tcgtgattga cgagttcggc
     5281 ctgttcagcg acgtgatgaa gaagtacggc atcgagaagg aatggaagtt ccctgtcaag
     5341 cccaagaaga agcggaaggt gggtggaggc ggaggttctg ggggaggagg tagtggcggt
     5401 ggtggttcag gaggcggcgg aagccagctg catttaccgc aggttttagc tgacgctgtc
     5461 tcacgcctgg tcctgggtaa gtttggtgac ctgaccgaca acttctcctc ccctcacgct
     5521 cgcagaaaag tgctggctgg agtcgtcatg acaacaggca cagatgttaa agatgccaag
     5581 gtgataagtg tttctacagg aggcaaatgt attaatggtg aatacatgag tgatcgtggc
     5641 cttgcattaa atgactgcca tgcagaaata atatctcgga gatccttgct cagatttctt
     5701 tatacacaac ttgagcttta cttaaataac aaagatgatc aaaaaagatc catctttcag
     5761 aaatcagagc gaggggggtt taggctgaag gagaatgtcc agtttcatct gtacatcagc
     5821 acctctccct gtggagatgc cagaatcttc tcaccacatg agccaatcct ggaagaacca
     5881 gcagatagac acccaaatcg taaagcaaga ggacagctac ggaccaaaat agagtctggt
     5941 caggggacga ttccagtgcg ctccaatgcg agcatccaaa cgtgggacgg ggtgctgcaa
     6001 ggggagcggc tgctcaccat gtcctgcagt gacaagattg cacgctggaa cgtggtgggc
     6061 atccagggat cactgctcag cattttcgtg gagcccattt acttctcgag catcatcctg
     6121 ggcagccttt accacgggga ccacctttcc agggccatgt accagcggat ctccaacata
     6181 gaggacctgc cacctctcta caccctcaac aagcctttgc tcagtggcat cagcaatgca
     6241 gaagcacggc agccagggaa ggcccccaac ttcagtgtca actggacggt aggcgactcc
     6301 gctattgagg tcatcaacgc cacgactggg aaggatgagc tgggccgcgc gtcccgcctg
     6361 tgtaagcacg cgttgtactg tcgctggatg cgtgtgcacg gcaaggttcc ctcccactta
     6421 ctacgctcca agattaccaa gcccaacgtg taccatgagt ccaagctggc ggcaaaggag
     6481 taccaggccg ccaaggcgcg tctgttcaca gccttcatca aggcggggct gggggcctgg
     6541 gtggagaagc ccaccgagca ggaccagttc tcactcacgt acccatacga cgtaccagat
     6601 tacgcttaag aattcctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
     6661 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     6721 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     6781 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagagaat agcaggcatg
     6841 ctggggagcg gccgctagtt attaatagta atcaattacg gggtcattag ttcatagccc
     6901 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     6961 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     7021 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     7081 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     7141 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     7201 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     7261 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     7321 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     7381 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
     7441 gatccgctag cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     7501 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     7561 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     7621 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     7681 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     7741 gcttcaagtg ggagcgcgtg atgaacttcg atgacggcgg cgtggtgacc gtgacccagg
     7801 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     7861 cctccgacgg ccccgtaatg cagaagaaaa ccatgggctg ggaggcctcc tccgagcgga
     7921 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     7981 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     8041 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     8101 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     8161 acaagtaaac cggtctcttg gcccggatcc ctgtgccttc tagttgccag ccatctgttg
     8221 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     8281 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     8341 gggtggggca ggacagcaag ggggaggatt gggaaaacaa tagcaggcat gctggggatg
     8401 cggtgggctc tatggaagct t
//
