LOCUS       Exported                7358 bp ds-DNA     circular SYN 04-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7358)
  AUTHORS   QingquanXiao
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 10, 2021 from SnapGene Viewer 5.2.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7358
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             106..966
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1137..1725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1819..2059
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    2100..2135
                     /label=13e1_DR
     enhancer        2158..2537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2538..2741
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          2833..2965
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             2998..3018
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3019..4350
                     /codon_start=1
                     /label=minidCas13X.1
                     /translation="LSMYCLKDSRFTKAWDKRVLLFRDILAQLGRIPAEAYEYYHGEQG
                     DKKRANDNEGTNPKRHKDKFIEFALHYLEAQHSEICFGRRHIVREEAGAGDEHKKHRTK
                     GKVVVDFSKKDEDQSYYISKNNVIVRIDKNAGPRSYRMGLNELKYLVLLSLQGKGDDAI
                     AKLYRYRQHVENILDVVKVTDKDNHVFLPRFVLEQHGIGRKAFKQRIDGRVKHVRGVWE
                     KKKAATNEMTLHEKARDILQYVNENCTRSFNPGEYNRLLVCLVGKDVENFQAGLKRLQL
                     AERIDGRVYSIFAQTSTINEMHQVVCDQILNRLCRIGDQKLYDYVGLGKKDEIDYKQKV
                     AWFKEHISIRRGFLRKKFWYDSKKGFAKLVEEHLESGGGQRDVGLDKKYYHIDAIGRFE
                     GANPALYETLARDRLCLMMAQYFLGSVRKELGNKIVWSNDSIELP"
     CDS             4351..4371
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4372..4434
                     /codon_start=1
                     /label=linker
                     /label=scFv GCN4
                     /translation="GGGGGSGGGGSGGGGSGGGGS"
     CDS             4435..5589
                     /codon_start=1
                     /label=REPAIRv2(hADAR2dd)
                     /translation="QLHLPQVLADAVSRLVLGKFGDLTDNFSSPHARRKVLAGVVMTTG
                     TDVKDAKVISVSTGGKCINGEYMSDRGLALNDCHAEIISRRSLLRFLYTQLELYLNNKD
                     DQKRSIFQKSERGGFRLKENVQFHLYISTSPCGDARIFSPHEPILEEPADRHPNRKARG
                     QLRTKIESGQGTIPVRSNASIQTWDGVLQGERLLTMSCSDKIARWNVVGIQGSLLSIFV
                     EPIYFSSIILGSLYHGDHLSRAMYQRISNIEDLPPLYTLNKPLLSGISNAEARQPGKAP
                     NFSVNWTVGDSAIEVINATTGKDELGRASRLCKHALYCRWMRVHGKVPSHLLRSKITKP
                     NVYHESKLAAKEYQAAKARLFTAFIKAGLGAWVEKPTEQDQFSLT"
     CDS             5590..5616
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    5650..5857
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        5866..6169
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6170..6373
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             6402..7121
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    7128..7352
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
       61 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      121 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      181 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      241 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      301 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      361 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      421 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      481 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
      541 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
      601 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
      661 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
      721 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
      781 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
      841 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
      901 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
      961 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1021 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1081 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1141 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1201 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1261 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1321 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1381 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1441 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1501 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1561 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1621 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1681 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1741 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1801 tcccctgatt ctctcgagga gggcctattt cccatgattc cttcatattt gcatatacga
     1861 tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta
     1921 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
     1981 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
     2041 tatatcttgt ggaaaggacg aaacaccggg gtcttcgata ttcaagcgtc ggaagacctg
     2101 ctggagcagc ccccgatttg tggggtgatt acagcttttt ttgtggacgg tgatatcggc
     2161 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     2221 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     2281 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     2341 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     2401 tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     2461 attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag
     2521 tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt
     2581 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     2641 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
     2701 gcggtaggcg tgtacggtgg gaggtctata taagcagagg tcgtttagtg aaccgtcaga
     2761 tcactagtag ctttattgcg gtagtttatc acagttaaat tgctaacgca gtcagtgctc
     2821 gactgatcac aggtaagtat caaggttaca agacaggttt aaggaggcca atagaaactg
     2881 ggcttgtcga gacagagaag attcttgcgt ttctgatagg cacctattgg tcttactgac
     2941 atccactttg cctttctctc cacaggggta ccgaagccgc tagtcgacgc caccatgccc
     3001 aagaagaagc ggaaggtgct gagcatgtac tgcctgaagg acagcagatt caccaaggcc
     3061 tgggataagc gggtgctgct gttcagagac atcctggccc agctgggaag aatccccgcc
     3121 gaggcctacg agtactacca cggcgagcag ggtgataaga agagagctaa cgacaatgag
     3181 ggcacaaatc ccaagcggca caaggacaag ttcatcgaat ttgcactgca ctacctggaa
     3241 gcccagcaca gcgagatctg cttcggcaga cgccacatcg tgcgggaaga ggccggcgcc
     3301 ggcgatgagc acaagaagca ccggaccaag ggaaaggtgg tggtggactt cagcaagaag
     3361 gacgaggacc agagctacta tatctccaag aacaacgtga tcgtgcggat cgacaagaac
     3421 gccggcccta gaagctaccg gatgggcctg aacgagctga agtacctcgt gctgctgagc
     3481 ctgcagggga agggcgacga tgccatcgcc aagctgtaca gatacagaca gcacgtggag
     3541 aacatcctgg atgtggtgaa ggtgaccgat aaggataacc acgtgttcct gccccgcttc
     3601 gtgctggagc agcacggcat cggcagaaag gccttcaagc agcggatcga tggacgggtg
     3661 aagcacgtgc ggggcgtgtg ggagaagaag aaggccgcca ccaatgaaat gaccctgcac
     3721 gagaaggcca gagacatcct gcagtacgtg aacgaaaact gcacccggtc cttcaaccct
     3781 ggcgaataca acagactgct ggtgtgcctg gtgggcaagg acgtggagaa ctttcaggcc
     3841 ggcctgaagc ggctgcagct ggccgaaagg atcgatggcc gggtgtactc catcttcgcc
     3901 cagaccagca ccatcaatga gatgcaccag gtggtgtgcg accagatcct gaaccggctg
     3961 tgcagaatcg gcgaccagaa gctgtacgat tacgtgggac tgggcaagaa ggacgaaatc
     4021 gactacaagc agaaggtggc ctggttcaag gagcacatca gcatccggag aggattcctg
     4081 agaaagaagt tctggtacga tagcaagaag ggattcgcaa agctggtgga ggaacacctg
     4141 gagtccggcg gcggccagcg cgacgtgggc ctggacaaga agtactacca catcgacgcc
     4201 atcggcagat tcgagggcgc caaccccgcc ctgtacgaga ccctggccag agatcggctg
     4261 tgcctcatga tggcccagta cttcctgggc agcgtgagaa aggaactggg caacaagatt
     4321 gtgtggagca acgacagcat cgaactgcct cccaagaaga agcggaaggt gggtggaggc
     4381 ggaggttctg ggggaggagg tagtggcggt ggtggttcag gaggcggcgg aagccagctg
     4441 catttaccgc aggttttagc tgacgctgtc tcacgcctgg tcctgggtaa gtttggtgac
     4501 ctgaccgaca acttctcctc ccctcacgct cgcagaaaag tgctggctgg agtcgtcatg
     4561 acaacaggca cagatgttaa agatgccaag gtgataagtg tttctacagg aggcaaatgt
     4621 attaatggtg aatacatgag tgatcgtggc cttgcattaa atgactgcca tgcagaaata
     4681 atatctcgga gatccttgct cagatttctt tatacacaac ttgagcttta cttaaataac
     4741 aaagatgatc aaaaaagatc catctttcag aaatcagagc gaggggggtt taggctgaag
     4801 gagaatgtcc agtttcatct gtacatcagc acctctccct gtggagatgc cagaatcttc
     4861 tcaccacatg agccaatcct ggaagaacca gcagatagac acccaaatcg taaagcaaga
     4921 ggacagctac ggaccaaaat agagtctggt caggggacga ttccagtgcg ctccaatgcg
     4981 agcatccaaa cgtgggacgg ggtgctgcaa ggggagcggc tgctcaccat gtcctgcagt
     5041 gacaagattg cacgctggaa cgtggtgggc atccagggat cactgctcag cattttcgtg
     5101 gagcccattt acttctcgag catcatcctg ggcagccttt accacgggga ccacctttcc
     5161 agggccatgt accagcggat ctccaacata gaggacctgc cacctctcta caccctcaac
     5221 aagcctttgc tcagtggcat cagcaatgca gaagcacggc agccagggaa ggcccccaac
     5281 ttcagtgtca actggacggt aggcgactcc gctattgagg tcatcaacgc cacgactggg
     5341 aaggatgagc tgggccgcgc gtcccgcctg tgtaagcacg cgttgtactg tcgctggatg
     5401 cgtgtgcacg gcaaggttcc ctcccactta ctacgctcca agattaccaa gcccaacgtg
     5461 taccatgagt ccaagctggc ggcaaaggag taccaggccg ccaaggcgcg tctgttcaca
     5521 gccttcatca aggcggggct gggggcctgg gtggagaagc ccaccgagca ggaccagttc
     5581 tcactcacgt acccatacga cgtaccagat tacgcttaag aattcctaga gctcgctgat
     5641 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     5701 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     5761 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     5821 gggaggattg ggaagagaat agcaggcatg ctggggagcg gccgccgtta cataacttac
     5881 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     5941 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     6001 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     6061 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     6121 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     6181 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     6241 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     6301 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     6361 tataagcaga gctggtttag tgaaccgtca gatccgctag catggtgagc aagggcgagg
     6421 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     6481 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     6541 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     6601 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     6661 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     6721 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     6781 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     6841 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     6901 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     6961 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     7021 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     7081 ccgccgggat cactctcggc atggacgagc tgtacaagta aggatccctg tgccttctag
     7141 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     7201 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     7261 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aaaacaatag
     7321 caggcatgct ggggatgcgg tgggctctat ggaagctt
//
