LOCUS       Exported                9288 bp ds-DNA     circular SYN 11-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 11, 2021 from SnapGene Viewer 5.2.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9288
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1768..2881
                     /codon_start=1
                     /label=MBP tag
                     /note="MBP tag"
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQTNSSS"
     CDS             2887..2910
                     /codon_start=1
                     /label=Strep tag
                     /note="Strep tag"
                     /translation="WSHPQFEK"
     CDS             2947..2970
                     /codon_start=1
                     /label=Strep tag
                     /note="Strep tag"
                     /translation="WSHPQFEK"
     CDS             2977..3000
                     /codon_start=1
                     /label=Flag tag
                     /note="Flag tag"
                     /translation="DYKDDDDK"
     CDS             3007..3027
                     /codon_start=1
                     /label=TEV cleavage site
                     /note="TEV cleavage site"
                     /translation="ENLYFQS"
     CDS             3043..6192
                     /codon_start=1
                     /label=hULK1
                     /translation="MEPGRGGTETVGKFEFSRKDLIGHGAFAVVFKGRHREKHDLEVAV
                     KCINKKNLAKSQTLLGKEIKILKELKHENIVALYDFQEMANSVYLVMEYCNGGDLADYL
                     HAMRTLSEDTIRLFLQQIAGAMRLLHSKGIIHRDLKPQNILLSNPAGRRANPNSIRVKI
                     ADFGFARYLQSNMMAATLCGSPMYMAPEVIMSQHYDGKADLWSIGTIVYQCLTGKAPFQ
                     ASSPQDLRLFYEKNKTLVPTIPRETSAPLRQLLLALLQRNHKDRMDFDEFFHHPFLDAS
                     PSVRKSPPVPVPSYPSSGSGSSSSSSSTSHLASPPSLGEMQQLQKTLASPADTAGFLHS
                     SRDSGGSKDSSCDTDDFVMVPAQFPGDLVAEAPSAKPPPDSLMCSGSSLVASAGLESHG
                     RTPSPSPPCSSSPSPSGRAGPFSSSRCGASVPIPVPTQVQNYQRIERNLQSPTQFQTPR
                     SSAIRRSGSTSPLGFARASPSPPAHAEHGGVLARKMSLGGGRPYTPSPQVGTIPERPGW
                     SGTPSPQGAEMRGGRSPRPGSSAPEHSPRTSGLGCRLHSAPNLSDLHVVRPKLPKPPTD
                     PLGAVFSPPQASPPQPSHGLQSCRNLRGSPKLPDFLQRNPLPPILGSPTKAVPSFDFPK
                     TPSSQNLLALLARQGVVMTPPRNRTLPDLSEVGPFHGQPLGPGLRPGEDPKGPFGRSFS
                     TSRLTDLLLKAAFGTQAPDPGSTESLQEKPMEIAPSAGFGGSLHPGARAGGTSSPSPVV
                     FTVGSPPSGSTPPQGPRTRMFSAGPTGSASSSARHLVPGPCSEAPAPELPAPGHGCSFA
                     DPITANLEGAVTFEAPDLPEETLMEQEHTEILRGLRFTLLFVQHVLEIAALKGSASEAA
                     GGPEYQLQESVVADQISLLSREWGFAEQLVLYLKVAELLSSGLQSAIDQIRAGKLCLSS
                     TVKQVVRRLNELYKASVVSCQGLSLRLQRFFLDKQRLLDRIHSITAERLIFSHAVQMVQ
                     SAALDEMFQHREGCVPRYHKALLLLEGLQHMLSDQADIENVTKCKLCIERRLSALLTGI
                     CA"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1621 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1681 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcctcgatc
     1741 gacggtatcg atgccaccat ggctagcatg aaaatcgaag aaggtaaact ggtaatctgg
     1801 attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat
     1861 accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt
     1921 gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac
     1981 gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat
     2041 ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt
     2101 gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa
     2161 gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac
     2221 ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag
     2281 tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg
     2341 ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac
     2401 tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg
     2461 gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc
     2521 aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt
     2581 ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg
     2641 gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag
     2701 ttggcgaaag atccacgtat tgccgccact atggaaaacg cccagaaagg tgaaatcatg
     2761 ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc
     2821 gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg
     2881 gctagctgga gccacccgca gttcgagaaa ggtggaggtg cccgaggtgg atcgggaggt
     2941 ggatcgtgga gccacccgca gttcgaaaaa ggattcgatt acaaggatga cgatgacaag
     3001 ggtaccgaaa acctgtactt ccaatccacc ggtggtcccg ggatggaacc tggcagagga
     3061 ggaacagaaa ccgtcggaaa attcgagttt agcagaaagg acctgattgg acatggagca
     3121 ttcgcagtcg tgttcaaggg tcgtcaccgg gaaaaacatg acctggaggt ggccgtcaag
     3181 tgcatcaaca agaaaaatct ggctaaatcc cagaccctgc tcggcaagga aatcaaaatt
     3241 ctgaaggagc tcaaacacga aaacattgtg gccctgtacg acttccagga aatggctaac
     3301 tctgtgtacc tggtcatgga gtattgcaat ggcggagacc tggctgatta tctccatgca
     3361 atgagaaccc tgagcgagga cactatccgt ctgttcctcc agcagattgc tggtgcaatg
     3421 cgtctgctcc actccaaggg catcattcat cgggatctga aaccccagaa catcctgctc
     3481 tccaatcctg ctggccgcag ggcaaaccca aattctatca gggtgaagat tgccgacttc
     3541 ggatttgcta gatacctgca gtctaacatg atggccgcta cactgtgcgg ttcacctatg
     3601 tacatggctc cagaggtcat tatgtcacag cactatgacg gaaaggccga tctgtggagt
     3661 atcggtacta ttgtgtacca gtgtctgaca ggcaaagccc ctttccaggc tagctcccca
     3721 caggacctgc gcctctttta tgaaaagaac aaaaccctgg tgccaactat cccccgtgag
     3781 acctctgcac ctctgcgtca gctgctcctg gcactcctgc agagaaatca caaggaccgt
     3841 atggacttcg atgagttctt tcaccatccc tttctggatg cttcacctag tgtgaggaaa
     3901 tccccccctg tgcctgtccc aagctaccca tcttcaggtt ccggcagtag ctcctcttca
     3961 agtagcacct ctcacctggc atccccacca tctctcggag agatgcagca gctgcagaag
     4021 accctcgcct cacccgctga cactgcagga ttcctgcatt cctctagaga ttcaggtggc
     4081 agtaaagact caagttgcga tactgacgat ttcgtgatgg tccccgctca gtttcctgga
     4141 gacctggtcg ccgaagctcc tagcgccaag cctccacccg attccctgat gtgttctggt
     4201 agctccctgg tggcatctgc aggtctcgag tcacacggtc gtaccccaag cccttcccct
     4261 ccatgctctt caagtccatc tccctcagga cgggcaggtc cattcagctc ctctcgctgt
     4321 ggcgcctctg tgcctatccc agtccccaca caggtgcaga actaccagcg gattgaacgc
     4381 aatctgcaga gcccaacaca gttccagacc ccccgttcaa gtgctatcag acgtagtggc
     4441 agcacctccc cactgggatt tgctcgtgca tctccatcac cacctgcaca cgctgagcat
     4501 ggaggtgtcc tggccaggaa gatgagtctc ggcggaggta gaccctatac acctagccca
     4561 caagtgggta ccattcctga aaggccaggc tggtcaggaa cccccagtcc tcagggtgct
     4621 gagatgaggg gcggaagatc cccacgtcca ggtagctccg caccagagca cagcccacgt
     4681 acttccggac tgggttgcag gctccacagt gctcctaacc tgagcgacct ccatgtggtc
     4741 cgtcccaagc tgcctaaacc acccaccgat cccctgggag cagtgttctc ccctccacag
     4801 gcatctccac ctcagccttc acatggactg cagagttgtc ggaacctccg cggtagccca
     4861 aagctgcccg acttcctcca gagaaatccc ctgccaccca tcctcggatc ccccacaaag
     4921 gccgtccctt ctttcgattt tcctaaaacc ccatcttcac agaacctcct ggccctcctg
     4981 gctcgtcagg gtgtggtcat gacacctcca cgcaatagga ccctgccaga cctctccgaa
     5041 gtgggtcctt tccacggaca gccactgggt cctggactcc gtccaggcga ggatccaaag
     5101 ggtcccttcg gccggagttt tagcacttcc cgcctgacag acctcctgct caaagcagcc
     5161 ttcggtacac aggcccctga tccaggcagc accgagtctc tgcaggagaa gccaatggag
     5221 atcgcaccct ctgccggctt tggtggttca ctgcacccag gagcaagggc aggaggtacc
     5281 agtagcccct cacctgtggt cttcactgtg ggaagtcccc cttctggttc aactccaccc
     5341 cagggcccta gaacacgtat gtttagtgct ggaccaactg gtagcgcatc ctcttcagcc
     5401 agacacctgg tcccaggacc ctgcagcgaa gctcctgcac cagagctgcc agcacctggt
     5461 catggttgtt ccttcgcaga ccctatcact gccaacctgg aaggcgctgt gacatttgag
     5521 gcacctgatc tgccagagga aaccctcatg gagcaggaac acactgagat cctgaggggt
     5581 ctcagattca ccctgctctt tgtgcagcat gtcctggaaa ttgctgcact caagggtagt
     5641 gctagcgagg cagctggtgg accagaatac cagctgcagg agagcgtggt cgccgaccag
     5701 atctctctgc tctcaaggga atggggcttc gctgagcagc tggtcctcta tctgaaggtg
     5761 gccgaactgc tcagtagcgg cctgcagtcc gccatcgatc agattagagc tggaaagctc
     5821 tgcctgtcct ctactgtgaa acaggtggtc cggcgcctga acgagctcta caaggccagt
     5881 gtggtcagct gtcagggact ctctctgcgt ctccagcggt tctttctgga caaacagcgc
     5941 ctgctcgata ggatccactc aattacagct gaacgcctga tcttcagcca tgcagtgcag
     6001 atggtccagt ccgcagccct ggacgaaatg tttcagcaca gggagggatg cgtccccaga
     6061 taccataagg ctctgctcct gctcgagggt ctgcagcaca tgctcagcga ccaggccgat
     6121 atcgaaaatg tgactaagtg caaactgtgt attgagagga gactctccgc tctgctcaca
     6181 ggcatctgtg cataacccgg gtgatatctt aattaattaa ctcgaggaat tcactcctca
     6241 ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata
     6301 ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca
     6361 tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     6421 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta
     6481 tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct
     6541 ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa
     6601 agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac
     6661 atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct cctgactact
     6721 cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc ccaagcttgg
     6781 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     6841 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     6901 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga
     6961 tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     7021 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7081 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     7141 aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa
     7201 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7261 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc
     7321 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     7381 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7441 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7501 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7561 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7621 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7681 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
     7741 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7801 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7861 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7921 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     7981 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     8041 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     8101 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     8161 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     8221 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8281 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8341 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8401 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8461 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8521 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8581 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     8641 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8701 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     8761 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     8821 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     8881 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     8941 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     9001 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     9061 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     9121 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     9181 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     9241 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctg
//
