LOCUS       Exported                6667 bp ds-DNA     circular SYN 10-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 10, 2021 from SnapGene Viewer 5.2.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6667
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    258..890
                     /label=GST
                     /note="GST"
     CDS             918..2648
                     /codon_start=1
                     /label=OPTN
                     /note="OPTN"
                     /translation="MSHQPLSCLTEKEDSPSESTGNGPPHLAHPNLDTFTPEELLQQMK
                     ELLTENHQLKEAMKLNNQAMKGRFEELSAWTEKQKEERQFFEIQSKEAKERLMALSHEN
                     EKLKEELGKLKGKSERSSEDPTDDSRLPRAEAEQEKDQLRTQVVRLQAEKADLLGIVSE
                     LQLKLNSSGSSEDSFVEIRMAEGEAEGSVKEIKHSPGPTRTVSTGTALSKYRSRSADGA
                     KNYFEHEELTVSQLLLCLREGNQKVERLEVALKEAKERVSDFEKKTSNRSEIETQTEGS
                     TEKENDEEKGPETVGSEVEALNLQVTSLFKELQEAHTKLSEAELMKKRLQEKCQALERK
                     NSAIPSELNEKQELVYTNKKLELQVESMLSEIKMEQAKTEDEKSKLTVLQMTHNKLLQE
                     HNNALKTIEELTRKESEKVDRAVLKELSEKLELAEKALASKQLQMDEMKQTIAKQEEDL
                     ETMTILRAQMEVYCSDFHAERAAREKIHEEKEQLALQLAVLLKENDAFEDGGRQSLMEM
                     QSRHGARTSDSDQQAYLVQRGAEDRDWRQQRNIPIHSCPKCGEVLPDIDTLQIHVMDCI
                     I"
ORIGIN
        1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
      301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
      361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
      421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
      481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
      541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
      601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
      661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
      721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
      781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
      841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
      901 atcctccaaa aatcgatatg tcccatcaac ctctcagctg cctcactgaa aaggaggaca
      961 gccccagtga aagcacagga aatggacccc cccacctggc ccacccaaac ctggacacgt
     1021 ttaccccgga ggagctgctg cagcagatga aagagctcct gaccgagaac caccagctga
     1081 aagaagccat gaagctaaat aatcaagcca tgaaagggag atttgaggag ctttcggcct
     1141 ggacagagaa acagaaggaa gaacgccagt tttttgagat acagagcaaa gaagcaaaag
     1201 agcgtctaat ggccttgagt catgagaatg agaaattgaa ggaagagctt ggaaaactaa
     1261 aagggaaatc agaaaggtca tctgaggacc ccactgatga ctccaggctt cccagggccg
     1321 aagcggagca ggaaaaggac cagctcagga cccaggtggt gaggctacaa gcagagaagg
     1381 cagacctgtt gggcatcgtg tctgaactgc agctcaagct gaactccagc ggctcctcag
     1441 aagattcctt tgttgaaatt aggatggctg aaggagaagc agaagggtca gtaaaagaaa
     1501 tcaagcatag tcctgggccc acgagaacag tctccactgg cacggcattg tctaaatata
     1561 ggagcagatc tgcagatggg gccaagaatt acttcgaaca tgaggagtta actgtgagcc
     1621 agctcctgct gtgcctaagg gaagggaatc agaaggtgga gagacttgaa gttgcactca
     1681 aggaggccaa agaaagagtt tcagattttg aaaagaaaac aagtaatcgt tctgagattg
     1741 aaacccagac agaggggagc acagagaaag agaatgatga agagaaaggc ccggagactg
     1801 ttggaagcga agtggaagca ctgaacctcc aggtgacatc tctgtttaag gagcttcaag
     1861 aggctcatac aaaactcagc gaagctgagc taatgaagaa gagacttcaa gaaaagtgtc
     1921 aggcccttga aaggaaaaat tctgcaattc catcagagtt gaatgaaaag caagagcttg
     1981 tttatactaa caaaaagtta gagctacaag tggaaagcat gctatcagaa atcaaaatgg
     2041 aacaggctaa aacagaggat gaaaagtcca aattaactgt gctacagatg acacacaaca
     2101 agcttcttca agaacataat aatgcattga aaacaattga ggaactaaca agaaaagagt
     2161 cagaaaaagt ggacagggca gtgctgaagg aactgagtga aaaactggaa ctggcagaga
     2221 aggctctggc ttccaaacag ctgcaaatgg atgaaatgaa gcaaaccatt gccaagcagg
     2281 aagaggacct ggaaaccatg accatcctca gggctcagat ggaagtttac tgttctgatt
     2341 ttcatgctga aagagcagcg agagagaaaa ttcatgagga aaaggagcaa ctggcattgc
     2401 agctggcagt tctgctgaaa gagaatgatg ctttcgaaga cggaggcagg cagtccttga
     2461 tggagatgca gagtcgtcat ggggcgagaa caagtgactc tgaccagcag gcttaccttg
     2521 ttcaaagagg agctgaggac agggactggc ggcaacagcg gaatattccg attcattcct
     2581 gccccaagtg tggagaggtt ctgcctgaca tagacacgtt acagattcac gtgatggatt
     2641 gcatcattta actcgagcgg ccgcatcgtg actgactgac gatctgcctc gcgcgtttcg
     2701 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     2761 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     2821 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtataattct tgaagacgaa
     2881 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     2941 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     3001 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     3061 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     3121 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     3181 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     3241 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     3301 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     3361 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     3421 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     3481 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     3541 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     3601 gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac
     3661 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     3721 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     3781 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     3841 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     3901 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     3961 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     4021 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     4081 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     4141 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     4201 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     4261 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     4321 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     4381 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     4441 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     4501 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     4561 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     4621 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     4681 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     4741 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     4801 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     4861 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     4921 cacaccgcat aaattccgac accatcgaat ggtgcaaaac ctttcgcggt atggcatgat
     4981 agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg
     5041 tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc
     5101 acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc
     5161 ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct
     5221 ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc
     5281 aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag
     5341 cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg
     5401 atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg
     5461 atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac
     5521 tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat
     5581 taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc
     5641 aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa
     5701 ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga
     5761 tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct
     5821 cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca
     5881 tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc
     5941 agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca
     6001 ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     6061 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     6121 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     6181 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacggattc
     6241 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     6301 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     6361 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc
     6421 agaagcggtg ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt
     6481 cccctcaaac tggcagatgc acggttacga tgcgcccatc tacaccaacg taacctatcc
     6541 cattacggtc aatccgccgt ttgttcccac ggagaatccg acgggttgtt actcgctcac
     6601 atttaatgtt gatgaaagct ggctacagga aggccagacg cgaattattt ttgatggcgt
     6661 tggaatt
//
