LOCUS       Exported                3763 bp ds-DNA     circular SYN 04-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 11, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
COMMENT     SECID/File created by SciEd Central, Scientific
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(63..167)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     641..657
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        664..682
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    664..680
                     /label=T7 promoter
     misc_feature    682
                     /label=5'UTR
     misc_feature    772
                     /label=5'UTR
     misc_feature    780..1439
                     /label=GST
     misc_feature    1440..1504
                     /label=MCS
     misc_feature    1440..1483
                     /label=MCS
                     /note="multiple cloning site of fluorescent protein 
                     plasmids"
     misc_feature    1506
                     /label=3'UTR
     promoter        complement(1769..1787)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1805..1821)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1829..1845
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1853..1883)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(2207..2796)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2967..3763)
                     /label=AmpR
ORIGIN
        1 aaacaggaag gcaaatgccg aaaaaaggga ataagggcga cacggaaatg ttgaatactc
       61 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
      121 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
      181 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
      241 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
      301 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
      361 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
      421 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
      481 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
      541 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      601 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
      661 ttgtaatacg actcactata gggcgaatta attcgagctc ggtacccagc ttgcttgttc
      721 tttttgcaga agctcagaat aaacgctcaa ctttggcaga tcaattcccc ggtgctagca
      781 tgtcccctat actaggttat tggaaaatta agggccttgt gcaacccact cgacttcttt
      841 tggaatatct tgaagaaaaa tatgaagagc atttgtatga gcgcgatgaa ggtgataaat
      901 ggcgaaacaa aaagtttgaa ttgggtttgg agtttcccaa tcttccttat tatattgatg
      961 gtgatgttaa attaacacag tctatggcca tcatacgtta tatagctgac aagcacaaca
     1021 tgttgggtgg ttgtccaaaa gagcgtgcag agatttcaat gcttgaagga gcggttttgg
     1081 atattagata cggtgtttcg agaattgcat atagtaaaga ctttgaaact ctcaaagttg
     1141 attttcttag caagctacct gaaatgctga aaatgttcga agatcgttta tgtcataaaa
     1201 catatttaaa tggtgatcat gtaacccatc ctgacttcat gttgtatgac gctcttgatg
     1261 ttgttttata catggaccca atgtgcctgg atgcgttccc aaaattagtt tgttttaaaa
     1321 aacgtattga agctatccca caaattgata agtacttgaa atccagcaag tatatagcat
     1381 ggcctttgca gggctggcaa gccacgtttg gtggtggcga ccatcctcca aaatcggatc
     1441 tcgagctcaa gcttcgaatt ctgcagtcga cggcccggga tcctgtacaa gtaaagcggc
     1501 cgctctagat aaatgatagc ttgatctggt taccactaaa ccagcctcaa gaacacccga
     1561 atggagtctc taagctacat aataccaact tacactttac aaaatgttgt cccccaaaat
     1621 gtagccattc gtatctgctc ctaataaaaa gaaagtttct tcacattcta aaaaaaaaaa
     1681 aaaaaaaaaa aaaaaaaaaa aaaccccccc cccccccccc tgcaggcgcg ccgccggcga
     1741 tttaaattaa ttaatctagc ttgagtattc tatagtgtca cctaaatagc ttggcgtaat
     1801 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     1861 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     1921 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     1981 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     2041 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     2101 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     2161 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2221 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2281 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2341 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2401 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2461 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2521 ccaacccggt aagacacgac cttatcgcca ctggcagcag ccactggtaa caggattagc
     2581 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     2641 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     2701 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2761 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2821 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     2881 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2941 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3001 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3061 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3121 ccggctccag atttatcagc aataaaccag ccagcccgga agggccgagc gcagaagtgg
     3181 tcctgcaaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     3241 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     3301 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     3361 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     3421 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     3481 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     3541 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     3601 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     3661 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     3721 gatcttcagc atcttttact ttcaccagcg tttctgggtg agc
//
