LOCUS       Exported                3939 bp ds-DNA     circular SYN 04-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 11, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
COMMENT     SECID/File created by SciEd Central, Scientific
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    664..680
                     /label=T7 promoter
     misc_feature    682
                     /label=5'UTR
     misc_feature    772
                     /label=5'UTR
     CDS             780..1433
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     misc_feature    1455..1631
                     /label=inserted from Cav002
     misc_feature    1470..1631
                     /label=Kash5Domain
     misc_feature    1682
                     /label=3'UTR
     CDS             complement(3143..3939)
                     /label=AmpR
ORIGIN
        1 aaacaggaag gcaaatgccg aaaaaaggga ataagggcga cacggaaatg ttgaatactc
       61 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
      121 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
      181 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
      241 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
      301 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
      361 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
      421 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
      481 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
      541 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      601 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
      661 ttgtaatacg actcactata gggcgaatta attcgagctc ggtacccagc ttgcttgttc
      721 tttttgcaga agctcagaat aaacgctcaa ctttggcaga tcaattcccc ggtgctagca
      781 tgtcccctat actaggttat tggaaaatta agggccttgt gcaacccact cgacttcttt
      841 tggaatatct tgaagaaaaa tatgaagagc atttgtatga gcgcgatgaa ggtgataaat
      901 ggcgaaacaa aaagtttgaa ttgggtttgg agtttcccaa tcttccttat tatattgatg
      961 gtgatgttaa attaacacag tctatggcca tcatacgtta tatagctgac aagcacaaca
     1021 tgttgggtgg ttgtccaaaa gagcgtgcag agatttcaat gcttgaagga gcggttttgg
     1081 atattagata cggtgtttcg agaattgcat atagtaaaga ctttgaaact ctcaaagttg
     1141 attttcttag caagctacct gaaatgctga aaatgttcga agatcgttta tgtcataaaa
     1201 catatttaaa tggtgatcat gtaacccatc ctgacttcat gttgtatgac gctcttgatg
     1261 ttgttttata catggaccca atgtgcctgg atgcgttccc aaaattagtt tgttttaaaa
     1321 aacgtattga agctatccca caaattgata agtacttgaa atccagcaag tatatagcat
     1381 ggcctttgca gggctggcaa gccacgtttg gtggtggcga ccatcctcca aaatcggatc
     1441 tcgagctcaa gcttgccgcc cccttcaccc acctcctgat cccagcgcca ctcctgggcc
     1501 tgctgctgct gctgctgctc tccgtcctgc tgctgggcca gtccccgccc cccacgtggc
     1561 cccacctcca gctctgctac ctccagcctc ctccagtgtg agccatgccc cctacccctg
     1621 cagagcaatg tgaattctgc agtcgacggc ccgggatcct gtacaagtaa agcggccgct
     1681 ctagataaat gatagcttga tctggttacc actaaaccag cctcaagaac acccgaatgg
     1741 agtctctaag ctacataata ccaacttaca ctttacaaaa tgttgtcccc caaaatgtag
     1801 ccattcgtat ctgctcctaa taaaaagaaa gtttcttcac attctaaaaa aaaaaaaaaa
     1861 aaaaaaaaaa aaaaaaaaac cccccccccc cccccctgca ggcgcgccgc cggcgattta
     1921 aattaattaa tctagcttga gtattctata gtgtcaccta aatagcttgg cgtaatcatg
     1981 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     2041 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     2101 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     2161 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     2221 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     2281 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2341 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2401 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2461 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2521 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2581 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2641 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2701 cccggtaaga cacgacctta tcgccactgg cagcagccac tggtaacagg attagcagag
     2761 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2821 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2881 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2941 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3001 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3061 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3121 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     3181 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     3241 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     3301 ctccagattt atcagcaata aaccagccag cccggaaggg ccgagcgcag aagtggtcct
     3361 gcaaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     3421 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     3481 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     3541 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     3601 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     3661 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     3721 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     3781 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     3841 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     3901 ttcagcatct tttactttca ccagcgtttc tgggtgagc
//
