LOCUS       G545A_pLenti-CMV-EGFP-2 8225 bp ds-DNA     circular     09-MAR-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        62..90
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(70..89)
                     /label="KAM1636"
                     /note="sequence: ttgtctcatgagcggataca"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     gene            132..992
                     /label="Ampicillin"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          144..166
                     /label="KAM483"
                     /note="sequence: catttccgtgtcgcccttattcc"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(144..166)
                     /label="KAM480"
                     /note="sequence: ggaataagggcgacacggaaatg"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(291..310)
                     /label="KAM849"
                     /note="sequence: gggcgaaaactctcaaggat"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          490..509
                     /label="KAM854"
                     /note="sequence: tatgcagtgctgccataacc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(823..842)
                     /label="KAM848"
                     /note="sequence: ctcaccggctccagatttat"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          983..1004
                     /label="KAM853"
                     /note="sequence: gcattggtaactgtcagaccaa"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     rep_origin      1147..1766
                     /label="pBR322_origin"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1310..1310
                     /label="C originally"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(1323..1342)
                     /label="KAM847"
                     /note="sequence: aagtggtggcctaactacgg"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          1493..1510
                     /label="KAM852"
                     /note="sequence: gtgcacacagcccagctt"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(1804..1823)
                     /label="KAM846"
                     /note="sequence: cgcaggaaagaacatgtgag"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          1828..1849
                     /label="KAM363"
                     /note="sequence: cccctgattCTGTGGATAACCG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          1978..1997
                     /label="KAM851"
                     /note="sequence: attaatgcagctggcacgac"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        2075..2104
                     /label="lac_promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(2105..2133)
                     /label="KAM2884"
                     /note="sequence: gaaattgttatccgctcacaattccacac"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          2134..2161
                     /label="KAM2885"
                     /note="sequence: acacaggaaacagctatgacCATGATTA"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(2170..2201)
                     /label="KAM1577"
                     /note="sequence: cttttgttccctttagtgagggttaattgcgc"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     promoter        2174..2193
                     /label="T3_promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          2174..2193
                     /label="KAM007-T3promoter"
                     /note="sequence: aattaaccctcactaaaggg"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          2186..2214
                     /label="KAM1576"
                     /note="sequence: ctaaagggaacaaaagctggagctgcaag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2218..2627
                     /label="RSV-5'LTR_hybrid_promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2447..2627
                     /label="HIV-1_5_LTR"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     misc_feature    2647..2647
                     /label="C to T"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2671..2673
                     /label="GAG inserted"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2738..2782
                     /label="HIV-1_psi_pack"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          2798..2817
                     /label="KAM767"
                     /note="sequence: TCAGTATTAAGCGGGGGAGA"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3292..3525
                     /label="RRE"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3346..3346
                     /label="C to G mut"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3612..3612
                     /label="T inserted"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          3966..3987
                     /label="KAM1452"
                     /note="sequence: gagacagagacagatccattcg"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4007..4012
                     /label="CGGTAT insertion"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    4025..4040
                     /label="cPPT"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(4047..4066)
                     /label="KAM517"
                     /note="sequence: TCTTTCCCCTGCACTGTACC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          4049..4075
                     /label="KAM1369"
                     /note="sequence: tacagtgcaggggaaagaatagtagac"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(4049..4075)
                     /label="KAM1368"
                     /note="sequence: gtctactattctttcccctgcactgta"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    4122..4123
                     /label="A deleted"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(4124..4156)
                     /label="KAM1164"
                     /note="sequence: aactcccaagcttatcgataaaattttgaattt"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        4157..4661
                     /label="CMV_immearly_promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          4241..4262
                     /label="KAM1773"
                     /note="sequence: tcccatagtaacgccaataggg"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(4241..4262)
                     /label="KAM1772"
                     /note="sequence: ccctattggcgttactatggga"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          4306..4325
                     /label="KAM3228"
                     /note="sequence: cccacttggcagtacatcaa"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          4457..4476
                     /label="KAM2144"
                     /note="sequence: TACCATGGTGATGCGGTTTT"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          4618..4638
                     /label="KAM020_CMVpromoter"
                     /note="sequence: CGCAAATGGGCGGTAGGCGTG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        4619..4688
                     /label="CMV_promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          4664..4688
                     /label="KAM024_LNCX-F"
                     /note="sequence: AGCTCGTTTAGTGAACCGTCAGATC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(4679..4698)
                     /label="KAM2063"
                     /note="sequence: gtctccaggcgatctgacgg"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(4685..4704)
                     /label="KAM1303"
                     /note="sequence: GGTggcgtctccaggcgatc"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(4685..4713)
                     /label="KAM1376"
                     /note="sequence: AGAcaccatggTGGCGTCTCCAGGCGATC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          4694..4734
                     /label="KAM1377"
                     /note="sequence: GAGACGCCAccatggtgTCTAAAGGAGAAGAATTATTTacc"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          4697..4721
                     /label="KAM1516"
                     /note="sequence: acGCCACCatggtgTCTAAAGGAGA"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          4698..4737
                     /label="KAM1943"
                     /note="sequence: CGCCACCatggtgTCTAAAGGAGAAGAATTATTTaccggg"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          4699..4737
                     /label="KAM1982"
                     /note="sequence: GCCACCatggtgTCTAAAGGAGAAGAATTATTTaccggg"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(4699..4753)
                     /label="KAM1206"
                     /note="sequence: ccaggatgggcaccaccccggtaaataattcttctcctttagacaccatggtggc"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             4705..6177
                     /label="Translation 4705-6177"
     primer          4708..4737
                     /label="KAM1222"
                     /note="sequence: gtgtctaaaggagaagaattatttaccggg"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(4708..4760)
                     /label="KAM1107"
                     /note="sequence: agctcgaccaggatgggcaccaccccggtAAATAATTCTTCTCCTTTAGAcac"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(4708..4765)
                     /label="KAM1027"
                     /note="sequence: cgtccagctcgaccaggatgggcaccaccccggtAAATAATTCTTCTCCTTTAGAcac"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    4708..5400
                     /label="EGFP"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          4712..4746
                     /label="KAM964"
                     /note="sequence: CTAAAGGAGAAGAATTATTTaccggggtggtgccc"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          4745..4765
                     /label="KAM1482"
                     /note="sequence: ccatcctggtcgagctggacg"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(4745..4765)
                     /label="KAM1483"
                     /note="sequence: cgtccagctcgaccaggatgg"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          4750..4771
                     /label="KAM262"
                     /note="sequence: ctggtcgagctggacggcgacg"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(4750..4771)
                     /label="KAM023_EGFP-N"
                     /note="sequence: CGTCGCCGTCCAGCTCGACCAG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          4803..4830
                     /label="KAM1026"
                     /note="sequence: gggcgagggcgatgccacctacggcaag"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(4813..4830)
                     /label="KAM1015"
                     /note="sequence: cttgccgtaggtggcatc"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(4836..4856)
                     /label="KAM047"
                     /note="sequence: gtggtgcagatgaacttcagg"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(4931..4951)
                     /label="KAM263"
                     /note="sequence: cgtgctgcttcatgtggtcgg"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          5093..5112
                     /label="KAM732"
                     /note="sequence: acttcaaggaggacggcaac"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(5210..5235)
                     /label="KAM1547"
                     /note="sequence: cacgctgccgtcctcgatgttgtggc"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          5222..5243
                     /label="KAM1546"
                     /note="sequence: aggacggcagcgtgcagctcgc"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          5245..5265
                     /label="KAM049"
                     /note="sequence: gaccactaccagcagaacacc"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(5247..5266)
                     /label="KAM733"
                     /note="sequence: gggtgttctgctggtagtgg"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          5358..5379
                     /label="KAM022_EGFP-C"
                     /note="sequence: CATGGTCCTGCTGGAGTTCGTG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(5383..5399)
                     /label="KAM998"
                     /note="sequence: agagtgatcccggcggc"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(5383..5400)
                     /label="KAM1485"
                     /note="sequence: gagagtgatcccggcggc"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          5384..5427
                     /label="KAM997"
                     /note="sequence: ccgccgggatcactctCGGAGGTagcggagctactaacttcagc"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5401..5412
                     /label="Linker"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(5407..5427)
                     /label="KAM1888"
                     /note="sequence: gctgaagttagtagctccgct"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          5407..5428
                     /label="KAM2735"
                     /note="sequence: agcggagctactaacttcagcc"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    5413..5469
                     /label="P2A"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          5413..5470
                     /label="KAM1877"
                     /note="sequence: GCTACTAACTTCAGCCTGCTGAAGCAGGCTGGAGACGTGGAGGAGAACCCTggacctg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          5429..5488
                     /label="KAM2719"
                     /note="sequence: TGCTGAAGCAGGCTGGAGACGTGGAGGAGAACCCTGGACCTGTGTCCAAGGGCGAGGAGG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(5430..5457)
                     /label="KAM1692"
                     /note="sequence: ctcctccacgtCTCCAGCCTGCTTCAGC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          5431..5461
                     /label="KAM485"
                     /note="sequence: ctgaagcaggctggaGacgtggaggagaacc"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(5431..5461)
                     /label="KAM486"
                     /note="sequence: ggttctcctccacgtCtccagcctgcttcag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(5436..5454)
                     /label="KAM1086"
                     /note="sequence: CTCCACGTCTCCAGCCTGC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(5439..5457)
                     /label="KAM1088"
                     /note="sequence: CTCCTCCACGTCTCCAGCC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          5439..5466
                     /label="KAM1691"
                     /note="sequence: GGCTGGAGacgtggaggagaaccctgga"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(5440..5460)
                     /label="KAM1090"
                     /note="sequence: GTTCTCCTCCACGTCTCCAGC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(5444..5463)
                     /label="KAM1092"
                     /note="sequence: AGGGTTCTCCTCCACGTCTC"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(5447..5466)
                     /label="KAM1064"
                     /note="sequence: tccagggttctcctccacgt"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(5450..5469)
                     /label="KAM1058"
                     /note="sequence: aggtccagggttctcctcca"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(5450..5478)
                     /label="KAM1378"
                     /note="sequence: CTTGGACACaggtccagggttctcctcca"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          5461..5488
                     /label="KAM1379"
                     /note="sequence: cctggacctGTGTCCAAGGGCGAGGAGG"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          5470..5489
                     /label="KAM3148"
                     /note="sequence: GTGTCCAAGGGCGAGGAGGA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5470..6174
                     /label="mNeonGreen"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          6154..6174
                     /label="KAM408"
                     /note="sequence: ggcatggacgagctgtacaag"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(6154..6174)
                     /label="KAM409"
                     /note="sequence: cttgtacagctcgtccatgcc"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(6154..6183)
                     /label="KAM1380"
                     /note="sequence: gggtgattaCTTGTACAGCTCGTCCATGCC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(6154..6184)
                     /label="KAM2172"
                     /note="sequence: tgggtgattaCTTGTACAGCTCGTCCATGCC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          6165..6201
                     /label="KAM2173"
                     /note="sequence: GCTGTACAAGtaatcacccagctttcttgtacaaagt"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          6166..6202
                     /label="KAM1381"
                     /note="sequence: CTGTACAAGtaatcacccagctttcttgtacaaagtg"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          6166..6214
                     /label="KAM1432"
                     /note="sequence: CTGTACAAGtaatcacccagctttcttgtacaaagtggttgatatccag"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          6175..6202
                     /label="KAM2062"
                     /note="sequence: taatcacccagctttcttgtacaaagtg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          6178..6202
                     /label="KAM1641"
                     /note="sequence: tcacccagctttcttgtacaaagtg"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          6205..6225
                     /label="KAM1302"
                     /note="sequence: tgatatccagcacagtggcgg"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(6228..6247)
                     /label="KAM1309"
                     /note="sequence: ccagaggttgattgtcgagc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    6237..6812
                     /label="WPRE"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(6310..6329)
                     /label="KAM1310"
                     /note="sequence: ggcattaaagcagcgtatcc"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(6843..6873)
                     /label="KAM2833"
                     /note="sequence: ACAGAATTAATTCCAAactcattactaaccg"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(6844..6873)
                     /label="KAM2848"
                     /note="sequence: acagaattaattccaaactcattactaacc"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          6856..6896
                     /label="KAM2847"
                     /note="sequence: gtttggaattaattctgtgcacaattcgagctcggtacctt"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          6858..6894
                     /label="KAM2832"
                     /note="sequence: tTGGAATTAATTCTGTgcacaattcgagctcggtacc"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          6875..6894
                     /label="KAM3217"
                     /note="sequence: cacaattcgagctcggtacc"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(6875..6894)
                     /label="KAM823"
                     /note="sequence: ggtaccgagctcgaattgtg"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6943..6964
                     /label="U3PPT"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(6959..6978)
                     /label="KAM1311"
                     /note="sequence: gagtgagctagcccttccag"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    7013..7193
                     /label="truncHIV-1_3_LTR"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    7059..7060
                     /label="GC to AA"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          7206..7253
                     /label="KAM797"
                     /note="sequence: gtcatcttattattcagtatttataacttgcaaagaaatgaatatcag"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          7268..7292
                     /label="KAM2768"
                     /note="sequence: TTGTTTATTGCAGCTTATAATGGTT"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     terminator      7268..7387
                     /label="SV40_PA_terminator"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          7302..7324
                     /label="KAM2148"
                     /note="sequence: GCAATAGCATCACAAATTTCACA"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          7345..7366
                     /label="KAM056"
                     /note="sequence: gcattctagttgtggtttgtcc"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(7345..7366)
                     /label="KAM202"
                     /note="sequence: GGACAAACCACAACTAGAATGC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(7356..7375)
                     /label="KAM191"
                     /note="sequence: GATGAGTTTGGACAAACCAC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    7405..7425
                     /label="Insertion"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     rep_origin      7426..7503
                     /label="SV40_origin"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          7545..7565
                     /label="KAM1219"
                     /note="sequence: AGGCTTTTTTGGAGGCCTAGG"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(7545..7565)
                     /label="KMA1220"
                     /note="sequence: CCTAGGCCTCCAAAAAAGCCT"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    7565..7575
                     /label="Deletion cttttgcgtcgagacgtaccc"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    7619..7762
                     /label="lacZ_a"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(7668..7685)
                     /label="KAM2198"
                     /note="sequence: gggatgtgctgcaaggcg"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(7686..7705)
                     /label="KAM2199"
                     /note="sequence: ttacgccagctggcgaaagg"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          7697..7716
                     /label="KAM856"
                     /note="sequence: ctggcgtaatagcgaagagg"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      7785..8091
                     /label="f1_origin"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          7895..7935
                     /label="KAM2515"
                     /note="sequence: CGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          8018..8037
                     /label="KAM1478"
                     /note="sequence: atagacggtttttcgccctt"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(8018..8037)
                     /label="KAM850"
                     /note="sequence: aagggcgaaaaaccgtctat"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          8081..8106
                     /label="KAM3188"
                     /note="sequence: aactggaacaacactcaaccctatct"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtT cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac Ataaacgggt ctctctggtt
     2461 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     2521 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     2581 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     2641 agggacTtga aagcgaaagg gaaaccaGAG GAGctctctc gacgcaggac tcggcttgct
     2701 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     2761 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     2821 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     2881 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     2941 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     3001 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     3061 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     3121 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     3181 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     3241 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     3301 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcGtcaa tgacgctgac
     3361 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     3421 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     3481 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     3541 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     3601 tctggaacag aTttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     3661 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     3721 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     3781 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     3841 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     3901 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     3961 agagagagac agagacagat ccattcgatt agtgaacgga tctcgaCGGT ATcggttaac
     4021 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     4081 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc
     4141 gataagcttg ggagttCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC
     4201 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG
     4261 GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC
     4321 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG
     4381 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG
     4441 TATTAGTCAT CGCTATTACC ATGGTGATGC GGTTTTGGCA GTACATCAAT GGGCGTGGAT
     4501 AGCGGTTTGA CTCACGGGGA TTTCCAAGTC TCCACCCCAT TGACGTCAAT GGGAGTTTGT
     4561 TTTGGCACCA AAATCAACGG GACTTTCCAA AATGTCGTAA CAACTCCGCC CCATTGACGC
     4621 AAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG CAGAGCTCGT TTAGTGAACC
     4681 GTCAGATCGC CTGGAGACGC CAccatggtg TCTAAAGGAG AAGAATTATT Taccggggtg
     4741 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     4801 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     4861 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     4921 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     4981 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     5041 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     5101 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     5161 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     5221 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     5281 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     5341 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctC
     5401 GGAGGTagcg gagctactaa cttcagcctg ctgaagcagg ctggagacgt ggaggagaac
     5461 cctggacctG TGTCCAAGGG CGAGGAGGAC AACATGGCCT CCCTGCCCGC TACCCACGAG
     5521 CTGCACATTT TCGGCTCCAT TAACGGCGTG GACTTCGACA TGGTGGGCCA GGGCACAGGC
     5581 AACCCAAACG ACGGCTACGA GGAGCTGAAC CTGAAGTCTA CAAAGGGCGA CCTGCAGTTC
     5641 TCTCCATGGA TCCTGGTGCC CCACATCGGC TACGGCTTCC ACCAGTACCT CCCCTACCCC
     5701 GACGGCATGT CCCCTTTCCA GGCCGCCATG GTGGACGGCA GCGGCTACCA GGTGCACAGG
     5761 ACCATGCAGT TCGAGGACGG CGCTAGCCTG ACAGTGAACT ACCGCTACAC CTACGAGGGC
     5821 AGCCACATTA AGGGCGAGGC TCAGGTGAAG GGCACAGGCT TCCCTGCCGA CGGCCCTGTG
     5881 ATGACAAACT CTCTGACAGC CGCTGACTGG TGCAGATCTA AGAAGACCTA CCCAAACGAC
     5941 AAGACCATCA TTAGCACCTT CAAGTGGTCT TACACCACCG GCAACGGCAA GCGGTACAGA
     6001 AGCACAGCCA GGACAACATA CACATTCGCT AAGCCCATGG CTGCTAACTA CCTGAAGAAC
     6061 CAGCCCATGT ACGTGTTCAG GAAGACAGAG CTGAAGCACA GCAAGACAGA GCTCAACTTC
     6121 AAGGAGTGGC AGAAGGCTTT CACCGACGTG ATGGGCATGG ACGAGCTGTA CAAGtaatca
     6181 cccagctttc ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgacaatcaa
     6241 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6301 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6361 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6421 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6481 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6541 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6601 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     6661 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6721 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     6781 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat tctgcagata
     6841 tccggttagt aatgagtttg gaattaattc tgtgcacaat tcgagctcgg tacctttaag
     6901 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     6961 ggaagggcta gctcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     7021 ctggttagac cagatctgag cctgggagct ctctggctAA ctagggaacc cactgcttaa
     7081 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7141 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     7201 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     7261 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7321 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7381 atcttatcat gtctggctct agctATCCCG CCCCTAACTC CGCCCatccc gcccctaact
     7441 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     7501 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7561 ctaggGACGT ACCCaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     7621 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     7681 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     7741 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     7801 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     7861 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     7921 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     7981 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     8041 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     8101 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     8161 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     8221 tttcc
//