LOCUS       Exported                7827 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    p15a-AsCas12f-blaZ
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 12, 2021 from SnapGene 3.1.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7827
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(534..1079)
                     /direction=LEFT
                     /note="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             1755..2615
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(2845..3723)
                     /label=araC
                     /note="araC"
     promoter        3750..4034
                     /gene="araBAD"
                     /note="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             4093..4509
                     /label=gam
                     /note="gam"
     CDS             4515..5300
                     /label=bet
                     /note="bet"
     CDS             5297..5977
                     /label=exo
                     /note="exo"
     promoter        6377..6558
                     /label=rpsL\promoter
                     /note="rpsL promoter"
     CDS             6559..7824
                     /codon_start=1
                     /label=AsCas12f1
                     /note="AsCas12f1"
                     /translation="MIKVYRYEIVKPLDLDWKEFGTILRQLQQETRFALNKATQLAWEW
                     MGFSSDYKDNHGEYPKSKDILGYTNVHGYAYHTIKTKAYRLNSGNLSQTIKRATDRFKA
                     YQKEILRGDMSIPSYKRDIPLDLIKENISVNRMNHGDYIASLSLLSNPAKQEMNVKRKI
                     SVIIIVRGAGKTIMDRILSGEYQVSASQIIHDDRKNKWYLNISYDFEPQTRVLDLNKIM
                     GIDLGVAVAVYMAFQHTPARYKLEGGEIENFRRQVESRRISMLRQGKYAGGARGGHGRD
                     KRIKPIEQLRDKIANFRDTTNHRYSRYIVDMAIKEGCGTIQMEDLTNIRDIGSRFLQNW
                     TYYDLQQKIIYKAEEAGIKVIKIDPQYTSQRCSECGNIDSGNRIGQAIFKCRACGYEAN
                     ADYNAARNIAIPNIDKIIAESIK"
ORIGIN
        1 tggctggttg gcgtactgtt gtggtaaata cccatatgga cagttttccc tttgatatgt
       61 aacggtgaac agttgttcta cttttgtttg ttagtcttga tgcttcactg atagatacaa
      121 gagcgtcggg tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct
      181 ccagtggctt ctgtttctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt
      241 aacggcaaaa gcaccgccgg acatcagcgc tagcggagtg tatactggct tactatgttg
      301 gcactgatga gggtgtcagt gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt
      361 gcgtcagcag aatatgtgat acaggatata ttccgcttcc tcgctcactg actcgctacg
      421 ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa cggggcggag atttcctgga
      481 agatgccagg aagatactta acagggaagt gagagggccg cggcaaagcc gtttttccat
      541 aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg gtggcgaaac
      601 ccgacaggac tataaagata ccaggcgttt ccccctggcg gctccctcgt gcgctctcct
      661 gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc
      721 acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc
      781 ccccgttcag tccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccgga
      841 aagacatgca aaagcaccac tggcagcagc cactggtaat tgatttagag gagttagtct
      901 tgaagtcatg cgccggttaa ggctaaactg aaaggacaag ttttggtgac tgcgctcctc
      961 caagccagtt acctcggttc aaagagttgg tagctcagag aaccttcgaa aaaccgccct
     1021 gcaaggcggt tttttcgttt tcagagcaag agattacgcg cagaccaaaa cgatctcaag
     1081 aagatcatct tattaatcag ataaaatatt tctagatttc agtgcaattt atctcttcaa
     1141 atgtagcacc tgaagtcagc cccatacgat ataagttgtg acgtaaaatg agacgttgat
     1201 cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt
     1261 tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
     1321 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
     1381 gttgctcaat gtacctataa ccagaccgtt cagctgggtc atccaccgga tcaattcccc
     1441 tgctcgcgca ggctgggtgc caagctctcg ggtaacatca aggcccgatc cttggagccc
     1501 ttgccctccc gcacgatgat cgtgccgtga tcgaaatcca gatccttgac ccgcagttgc
     1561 aaaccctcac tgatccggct cacggtaact gatgccgtat ttgcagtacc agcgtacggc
     1621 ccacagaatg atgtcacgct gaaaatgccg gcctttgaat gggttcatgt gcagctccat
     1681 cagcaaaagg ggatgataag tttatcacca ccgactattt gcaacagtgc cgttgatcgt
     1741 gctatgatcg actgatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     1801 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     1861 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     1921 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     1981 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     2041 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     2101 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     2161 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     2221 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     2281 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     2341 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     2401 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     2461 gtgagcgtgg gtcgcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     2521 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     2581 ctgagatagg tgcctcactg attaagcatt ggtaatgagc tcatgagcgg agaacgagat
     2641 gacgttggag gggcaaggtc gcgctgattg ctggggcaac acgtgaaagg cgagatcacc
     2701 aaggtagtcg gcaaataatg tctaacggta cccggggatc ctctagagtc gacctgcata
     2761 atgtgcctgt caaatggacg aagcagggat tctgcaaacc ctatgctact ccgtcaagcc
     2821 gtcaattgtc tgattcgtta ccaattatga caacttgacg gctacatcat tcactttttc
     2881 ttcacaaccg gcacggaact cgctcgggct ggccccggtg cattttttaa atacccgcga
     2941 gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag gcatccgggt
     3001 ggtgctcaaa agcagcttcg cctggctgat acgttggtcc tcgcgccagc ttaagacgct
     3061 aatccctaac tgctggcgga aaagatgtga cagacgcgac ggcgacaagc aaacatgctg
     3121 tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt actgacaagc
     3181 ctcgcgtacc cgattatcca tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg
     3241 cagtaacaat tgctcaagca gatttatcgc cagcagctcc gaatagcgcc cttccccttg
     3301 cccggcgtta atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg
     3361 gcgaaagaac cccgtattgg caaatattga cggccagtta agccattcat gccagtaggc
     3421 gcgcggacga aagtaaaccc actggtgata ccattcgcga gcctccggat gacgaccgta
     3481 gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg
     3541 tccctgattt ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat aacctttcat
     3601 tcccagcggt cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc
     3661 cgccaccaga tgggcattaa acgagtatcc cggcagcagg ggatcatttt gcgcttcagc
     3721 catacttttc atactcccgc cattcagaga agaaaccaat tgtccatatt gcatcagaca
     3781 ttgccgtcac tgcgtctttt actggctctt ctcgctaacc aaaccggtaa ccccgcttat
     3841 taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt
     3901 ctataatcac ggcagaaaag tccacattga ttatttgcac ggcgtcacac tttgctatgc
     3961 catagcattt ttatccataa gattagcgga tcctacctga cgctttttat cgcaactctc
     4021 tactgtttct ccatacccgt ttttttgggc tagcgaaaag atgtttcgtg aagccgtcga
     4081 cgcttataaa aaatggatat taatactgaa actgagatca agcaaaagca ttcactaacc
     4141 ccctttcctg ttttcctaat cagcccggca tttcgcgggc gatattttca cagctatttc
     4201 aggagttcag ccatgaacgc ttattacatt caggatcgtc ttgaggctca gagctgggcg
     4261 cgtcactacc agcagctcgc ccgtgaagag aaagaggcag aactggcaga cgacatggaa
     4321 aaaggcctgc cccagcacct gtttgaatcg ctatgcatcg atcatttgca acgccacggg
     4381 gccagcaaaa aatccattac ccgtgcgttt gatgacgatg ttgagtttca ggagcgcatg
     4441 gcagaacaca tccggtacat ggttgaaacc attgctcacc accaggttga tattgattca
     4501 gaggtataaa acgaatgagt actgcactcg caacgctggc tgggaagctg gctgaacgtg
     4561 tcggcatgga ttctgtcgac ccacaggaac tgatcaccac tcttcgccag acggcattta
     4621 aaggtgatgc cagcgatgcg cagttcatcg cattactgat cgttgccaac cagtacggcc
     4681 ttaatccgtg gacgaaagaa atttacgcct ttcctgataa gcagaatggc atcgttccgg
     4741 tggtgggcgt tgatggctgg tcccgcatca tcaatgaaaa ccagcagttt gatggcatgg
     4801 actttgagca ggacaatgaa tcctgtacat gccggattta ccgcaaggac cgtaatcatc
     4861 cgatctgcgt taccgaatgg atggatgaat gccgccgcga accattcaaa actcgcgaag
     4921 gcagagaaat cacggggccg tggcagtcgc atcccaaacg gatgttacgt cataaagcca
     4981 tgattcagtg tgcccgtctg gccttcggat ttgctggtat ctatgacaag gatgaagccg
     5041 agcgcattgt cgaaaatact gcatacactg cagaacgtca gccggaacgc gacatcactc
     5101 cggttaacga tgaaaccatg caggagatta acactctgct gatcgccctg gataaaacat
     5161 gggatgacga cttattgccg ctctgttccc agatatttcg ccgcgacatt cgtgcatcgt
     5221 cagaactgac acaggccgaa gcagtaaaag ctcttggatt cctgaaacag aaagccgcag
     5281 agcagaaggt ggcagcatga caccggacat tatcctgcag cgtaccggga tcgatgtgag
     5341 agctgtcgaa cagggggatg atgcgtggca caaattacgg ctcggcgtca tcaccgcttc
     5401 agaagttcac aacgtgatag caaaaccccg ctccggaaag aagtggcctg acatgaaaat
     5461 gtcctacttc cacaccctgc ttgctgaggt ttgcaccggt gtggctccgg aagttaacgc
     5521 taaagcactg gcctggggaa aacagtacga gaacgacgcc agaaccctgt ttgaattcac
     5581 ttccggcgtg aatgttactg aatccccgat catctatcgc gacgaaagta tgcgtaccgc
     5641 ctgctctccc gatggtttat gcagtgacgg caacggcctt gaactgaaat gcccgtttac
     5701 ctcccgggat ttcatgaagt tccggctcgg tggtttcgag gccataaagt cagcttacat
     5761 ggcccaggtg cagtacagca tgtgggtgac gcgaaaaaat gcctggtact ttgccaacta
     5821 tgacccgcgt atgaagcgtg aaggcctgca ttatgtcgtg attgagcggg atgaaaagta
     5881 catggcgagt tttgacgaga tcgtgccgga gttcatcgaa aaaatggacg aggcactggc
     5941 tgaaattggt tttgtatttg gggagcaatg gcgatgacgc atcctcacga taataagctt
     6001 cctgctgaac atcaaaggca agaaaacatc tgttgtcaaa gacagcatcc ttgaacaagg
     6061 acaattaaca gttaacaaat aaaaacgcaa aagaaaatgc cgatattgac taccggaagc
     6121 agtgtgaccg tgtgcttctc aaatgcctga ttcaggctgt ctatgtgtga ctgttgagct
     6181 gtaacaagtt gtctcaggtg ttcaatttca tgttctagtt gctttgtttt actggtttca
     6241 cctgttctat taggtgttac atgctgttca tctgttacat tgtcgatctg ttcatggtga
     6301 acagctttaa atgcaccaaa aactcgtaaa agctctgatg tatctatctt ttttacaccg
     6361 ttttcatctg tgcatatagc tatactgatt tcgtcagact cacagtcaaa catgccggtc
     6421 agttggcctg gtgatggcgg gatcgttgta tatttcttga caccttttcg gcaccgccct
     6481 aaaattctgc gtcctcataa tatatgaggc gatttattac gtgtttacga agcaaaagct
     6541 aaaaccagga gctatttaat gatcaaagtt taccgttatg aaatcgttaa accgctggac
     6601 ctggattgga aagaattcgg caccatcctg cgtcagctgc agcaggaaac ccgcttcgcg
     6661 ctgaacaaag caacccagct ggcgtgggaa tggatgggtt tcagctctga ttacaaagac
     6721 aaccatggcg aatacccgaa aagcaaagac atcctgggct acaccaacgt tcacggttac
     6781 gcataccaca ccatcaaaac caaagcttat cgtctgaaca gcggcaacct gagccagacc
     6841 atcaaacgtg cgaccgatcg cttcaaagcg taccagaaag aaatcctgcg tggtgacatg
     6901 tccatcccgt cttacaaacg tgatatcccg ctggatctga tcaaagaaaa catcagcgtt
     6961 aaccgtatga accacggtga ttatatcgct tccctgtccc tgctgtctaa cccggcgaaa
     7021 caggaaatga acgttaaacg taaaatctcc gtgattatca tcgttcgtgg cgcgggtaaa
     7081 accatcatgg atcgcatcct gagcggcgaa taccaggtga gcgcgagcca gatcatccac
     7141 gacgaccgta aaaacaaatg gtacctgaac atctcttatg atttcgaacc gcagacccgt
     7201 gttctggatc tgaacaaaat catgggcatc gatctgggtg ttgcggtggc ggtgtacatg
     7261 gcgttccagc acaccccggc gcgctacaaa ctggaaggcg gtgaaatcga aaacttccgt
     7321 cgtcaggtgg agtctcgtcg catctccatg ctgcgccagg gtaaatacgc gggtggtgct
     7381 cgtggcggtc acggccgtga taaacgtatc aaaccgatcg aacagctgcg tgacaaaatc
     7441 gctaacttcc gcgataccac taaccaccgt tactctcgtt acatcgttga tatggctatc
     7501 aaagaaggtt gcggtaccat tcagatggaa gatctgacca acatccgtga tatcggctct
     7561 cgtttcctgc agaactggac ctactacgat ctgcagcaga aaatcatcta caaagcggaa
     7621 gaagcgggca tcaaagttat caaaattgat ccgcagtaca ccagccagcg ttgcagcgaa
     7681 tgcggcaaca tcgattccgg taaccgtatc ggtcaggcga tcttcaaatg ccgtgcttgc
     7741 ggctacgaag cgaacgcgga ctacaacgca gctcgtaaca tcgcgatccc gaacatcgat
     7801 aaaatcatcg cggaaagcat caaataa
//
