LOCUS       Exported                3211 bp ds-DNA     circular SYN 14-MAY-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 14, 2021 from SnapGene 5.2.5.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     28..46
                     /label=T7
                     /note="color: #00ffff; direction: RIGHT"
     misc_feature    28..44
                     /label=T7 Promoter
                     /note="color: #00ff00"
     misc_binding    47..69
                     /label=LacO
                     /note="color: #6495ed"
     primer_bind     95..120
                     /label=Primer 1
                     /note="Forward primer for cloning into 
                     T7-TetO-TetR_intermediate."
                     /note="color: black; sequence: 
                     AGTACTCTTTAAGAAGGAGGAGTACATATGCC; added: 2020-03-29"
     misc_feature    95..107
                     /label=RBS
                     /note="color: #ff0080"
     CDS             119..1168
                     /codon_start=1
                     /label=QF
                     /note="color: #800080"
                     /translation="PPKRKTLNAAAEANAHADGHADGNADGHVANTAASSNNARFADLT
                     NIDTPGLGPTTTTLLVEPARSKRQRVSRACDQCRAAREKCDGIQPACFPCVSQGRSCTY
                     QASPKKRGVQTGYIRTLELALAWMFENVARSEDALHNLLVRDAGQGSALLVGKDSPAAE
                     RLHARWATSRVNKSITRLLRQLELPPTATATASIMPHVMEQPLSTSINPVNDRFNGIPN
                     PTPYNSDAALDAITQTNDYGSVNTHGILSTYPPPATHLNEASVALAPGGAPPRPPPPYV
                     DSTTNHPPYHSNLVPMANFGYSTVDYDAMVDDLASIEYTDAVDVDPQFMTNLGFVPGCN
                     FSDINTYEQ"
     primer_bind     complement(1144..1168)
                     /label=Primer 2
                     /note="Reverse primer for cloning into 
                     T7-TetO-TetR_Intermediate"
                     /note="color: black; sequence: 
                     CCTGCAGGTCACTGTTCGTATGTATTAATGTCG; added: 2020-03-29"
     misc_feature    1175..1222
                     /label=T7 stop
                     /note="color: #800000"
     misc_feature    1231..2143
                     /label=p15A Origin
                     /note="color: #8080ff"
     misc_feature    2150..2244
                     /label=T0
                     /note="color: #ff8000"
     CDS             complement(2275..3066)
                     /label=Kan/neoR
                     /note="color: #ffff00; direction: LEFT"
ORIGIN
        1 gaattccgct agccgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg
       61 ataacaattc ccctctagaa ataattttgt ttaactttaa gaaggaggag tacatatgcc
      121 acccaagcgc aaaacgctta acgctgcggc tgaggctaac gctcatgccg acggacacgc
      181 cgacggaaac gccgacggac acgtggccaa tacggccgcg tcctcgaata atgcgaggtt
      241 cgctgatctc actaacatcg atactccggg tctgggaccc acaactacga ccctgctcgt
      301 ggaaccagca cgctcaaagc gtcaacgagt gtcccgcgca tgcgaccagt gccgtgcagc
      361 ccgagagaaa tgcgacggaa tacagcctgc gtgtttcccg tgcgtttccc agggaaggtc
      421 ctgcacttat caggcttcgc cgaaaaagag gggagttcaa accggttata ttcgtacgct
      481 ggagctcgcc ctcgcctgga tgtttgaaaa tgtcgcgcgt tccgaagatg ccttgcataa
      541 cctcctcgtc cgtgacgccg gacaaggatc agctctgctc gttggtaaag attcgccggc
      601 tgccgagcga ctccatgccc gttgggctac tagccgtgtc aataagagca ttacccgcct
      661 cctccgtcag ttggagctcc ctcctaccgc cacggctacg gcctcgataa tgccgcacgt
      721 gatggagcag cctctcagta ccagcattaa ccccgtcaac gaccgcttca acggtattcc
      781 caaccccact ccgtataact ccgatgcagc tctcgatgct atcactcaga ccaacgatta
      841 tggaagcgta aatacacatg gtatcctctc tacttacccg ccaccggcta cgcaccttaa
      901 tgaagcttcc gtcgctctcg ctcccggtgg cgcccccccc cgaccgcctc ctccgtatgt
      961 tgacagcacg accaatcacc cgccgtacca ctcgaatctg gttccaatgg cgaactttgg
     1021 ttactcgacc gttgattacg atgccatggt tgacgatttg gctagcattg aatacacgga
     1081 cgctgtggat gtcgacccac agtttatgac caatctggga ttcgttcctg gatgtaactt
     1141 ctccgacatt aatacatacg aacagtgaaa gcttctagca taaccccttg gggcctctaa
     1201 acgggtcttg aggggttttt tggcggccgc acaacttata tcgtatgggg ctgacttcag
     1261 gtgctacatt tgaagagata aattgcactg aaatctagaa atattttatc tgattaataa
     1321 gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa
     1381 ccgccttgca gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta
     1441 actggcttgg aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc
     1501 atgacttcaa gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt
     1561 gcatgtcttt ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac
     1621 tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt
     1681 gtcaggcgtg gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa
     1741 ggcaggaaca ggagagcgca cgagggagcc gccaggggga aacgcctggt atctttatag
     1801 tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg
     1861 gcggagccta tggaaaaacg gctttgccgc ggccctctca cttccctgtt aagtatcttc
     1921 ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga
     1981 acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atatcctgta tcacatattc
     2041 tgctgacgca ccggtgcagc cttttttctc ctgccacatg aagcacttca ctgacaccct
     2101 catcagtgcc aacatagtaa gccagtatac actccgctag cgcactagta ttctcaccaa
     2161 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
     2221 ttactggatc tatcaacagg agtccaagcg agctctcgaa ccccagagtc ccgctcagaa
     2281 gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta
     2341 aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc
     2401 caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga
     2461 aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag
     2521 atcctcgccg tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc
     2581 ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc
     2641 tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg
     2701 cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga
     2761 caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac
     2821 aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc
     2881 ctcgtcctgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg
     2941 cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca
     3001 gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg
     3061 ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca gatcttgatc ccctgcgcca
     3121 tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc
     3181 agagggcgcc ccagctggca attccgacgt c
//
