LOCUS       Exported                4079 bp ds-DNA     circular SYN 10-SEP-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 14, 2021 from SnapGene 5.2.5.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     87..105
                     /label=T7
                     /note="color: #00ffff; direction: RIGHT"
     misc_feature    87..103
                     /label=T7 Promoter
                     /note="color: #00ff00"
     misc_binding    106..128
                     /label=LacO
                     /note="color: #6495ed"
     misc_feature    154..166
                     /label=RBS
                     /note="color: #ff0080"
     CDS             175..831
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /note="color: #993366"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSGSREFRSY"
     misc_feature    838..885
                     /label=T7 stop
                     /note="color: #800000"
     misc_feature    894..1806
                     /label=p15A Origin
                     /note="color: #8080ff"
     misc_feature    1813..1907
                     /label=T0
                     /note="color: #ff8000"
     CDS             complement(1938..2729)
                     /label=Kan/neoR
                     /note="color: #ffff00; direction: LEFT"
     primer_bind     2811..2832
                     /label=Primer 1
                     /note="Forward primer. NotI site added"
                     /note="color: black; sequence: 
                     GCGGCCGCCAGTTTACTTTGCAGGGCTTC; added: 2020-09-11"
     primer_bind     2811..2829
                     /label=Primer 1 (1)
                     /note="color: black; sequence: 
                     TCAGTGCGGCCGCCAGTTTACTTTGCAGGGC; added: 2020-09-11"
     promoter        2884..2902
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    2903..2921
                     /gene="tetO"
                     /allele="Tet operator (TetO2 from Tn10). TetR binds here to
                     suppress transcription."
                     /label=TetO
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     primer_bind     2925..2938
                     /label=Primer 1
                     /note="Forward primer. NotI site added"
                     /note="color: black; sequence: 
                     GCGGCCGCCAGTTTACTTTGCAGGGCTTC; added: 2020-09-11"
     primer_bind     2925..2935
                     /label=Primer 1 (1)
                     /note="color: black; sequence: 
                     TCAGTGCGGCCGCCAGTTTACTTTGCAGGGC; added: 2020-09-11"
     misc_feature    2950..2962
                     /label=RBS
                     /note="color: #ff0080"
     CDS             2971..4023
                     /codon_start=1
                     /label=QF
                     /note="color: #800080"
                     /translation="MPPKRKTLNAAAEANAHADGHADGNADGHVANTAASSNNARFADL
                     TNIDTPGLGPTTTTLLVEPARSKRQRVSRACDQCRAAREKCDGIQPACFPCVSQGRSCT
                     YQASPKKRGVQTGYIRTLELALAWMFENVARSEDALHNLLVRDAGQGSALLVGKDSPAA
                     ERLHARWATSRVNKSITRLLRQLELPPTATATASIMPHVMEQPLSTSINPVNDRFNGIP
                     NPTPYNSDAALDAITQTNDYGSVNTHGILSTYPPPATHLNEASVALAPGGAPPRPPPPY
                     VDSTTNHPPYHSNLVPMANFGYSTVDYDAMVDDLASIEYTDAVDVDPQFMTNLGFVPGC
                     NFSDINTYEQ"
     misc_feature    4030..4077
                     /label=T7 stop
                     /note="color: #800000"
     primer_bind     join(4078..4079,1..27)
                     /label=Primer 1
                     /note="Forward primer. NotI site added"
                     /note="color: black; sequence: 
                     GCGGCCGCCAGTTTACTTTGCAGGGCTTC; added: 2020-09-11"
     primer_bind     join(4078..4079,1..24)
                     /label=Primer 1 (1)
                     /note="color: black; sequence: 
                     TCAGTGCGGCCGCCAGTTTACTTTGCAGGGC; added: 2020-09-11"
ORIGIN
        1 ggccgccagt ttactttgca gggcttccca accttaccag agggcgcccc agctggcaat
       61 tccgacgtcc gaaatccatg gtcacttaat acgactcact ataggggaat tgtgagcgga
      121 taacaattcc cgactagaaa taattttgtt taactttaag aaggaggagg atccatgtct
      181 agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc
      241 gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat
      301 tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg
      361 caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac
      421 gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt
      481 acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa
      541 caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta
      601 ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact
      661 actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca
      721 gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa
      781 tgtgaaagtg ggtccgcgta cagcggatcc cgggaattca gatcttatta aaagcttcta
      841 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttggcggc cgcacaactt
      901 atatcgtatg gggctgactt caggtgctac atttgaagag ataaattgca ctgaaatcta
      961 gaaatatttt atctgattaa taagatgatc ttcttgagat cgttttggtc tgcgcgtaat
     1021 ctcttgctct gaaaacgaaa aaaccgcctt gcagggcggt ttttcgaagg ttctctgagc
     1081 taccaactct ttgaaccgag gtaactggct tggaggagcg cagtcaccaa aacttgtcct
     1141 ttcagtttag ccttaaccgg cgcatgactt caagactaac tcctctaaat caattaccag
     1201 tggctgctgc cagtggtgct tttgcatgtc tttccgggtt ggactcaaga cgatagttac
     1261 cggataaggc gcagcggtcg gactgaacgg ggggttcgtg catacagtcc agcttggagc
     1321 gaactgccta cccggaactg agtgtcaggc gtggaatgag acaaacgcgg ccataacagc
     1381 ggaatgacac cggtaaaccg aaaggcagga acaggagagc gcacgaggga gccgccaggg
     1441 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc accactgatt tgagcgtcag
     1501 atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa acggctttgc cgcggccctc
     1561 tcacttccct gttaagtatc ttcctggcat cttccaggaa atctccgccc cgttcgtaag
     1621 ccatttccgc tcgccgcagt cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg
     1681 aatatatcct gtatcacata ttctgctgac gcaccggtgc agcctttttt ctcctgccac
     1741 atgaagcact tcactgacac cctcatcagt gccaacatag taagccagta tacactccgc
     1801 tagcgcacta gtattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
     1861 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctctc
     1921 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
     1981 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc
     2041 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc
     2101 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag
     2161 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg
     2221 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga
     2281 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg
     2341 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc
     2401 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc
     2461 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg
     2521 gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg
     2581 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag
     2641 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga
     2701 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga
     2761 tcagatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact
     2821 ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccga cgtcccgata
     2881 aattaatacg actcactata ggtccctatc agtgatagag agttctttgc agggcttccc
     2941 aacctttaac tttaagaagg aggaggatcc atgccaccca agcgcaaaac gcttaacgct
     3001 gcggctgagg ctaacgctca tgccgacgga cacgccgacg gaaacgccga cggacacgtg
     3061 gccaatacgg ccgcgtcctc gaataatgcg aggttcgctg atctcactaa catcgatact
     3121 ccgggtctgg gacccacaac tacgaccctg ctcgtggaac cagcacgctc aaagcgtcaa
     3181 cgagtgtccc gcgcatgcga ccagtgccgt gcagcccgag agaaatgcga cggaatacag
     3241 cctgcgtgtt tcccgtgcgt ttcccaggga aggtcctgca cttatcaggc ttcgccgaaa
     3301 aagaggggag ttcaaaccgg ttatattcgt acgctggagc tcgccctcgc ctggatgttt
     3361 gaaaatgtcg cgcgttccga agatgccttg cataacctcc tcgtccgtga cgccggacaa
     3421 ggatcagctc tgctcgttgg taaagattcg ccggctgccg agcgactcca tgcccgttgg
     3481 gctactagcc gtgtcaataa gagcattacc cgcctcctcc gtcagttgga gctccctcct
     3541 accgccacgg ctacggcctc gataatgccg cacgtgatgg agcagcctct cagtaccagc
     3601 attaaccccg tcaacgaccg cttcaacggt attcccaacc ccactccgta taactccgat
     3661 gcagctctcg atgctatcac tcagaccaac gattatggaa gcgtaaatac acatggtatc
     3721 ctctctactt acccgccacc ggctacgcac cttaatgaag cttccgtcgc tctcgctccc
     3781 ggtggcgccc ccccccgacc gcctcctccg tatgttgaca gcacgaccaa tcacccgccg
     3841 taccactcga atctggttcc aatggcgaac tttggttact cgaccgttga ttacgatgcc
     3901 atggttgacg atttggctag cattgaatac acggacgctg tggatgtcga cccacagttt
     3961 atgaccaatc tgggattcgt tcctggatgt aacttctccg acattaatac atacgaacag
     4021 tgaaagcttc tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttggc
//
