LOCUS       Exported                3989 bp ds-DNA     circular SYN 23-APR-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 14, 2021 from SnapGene 5.2.5.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     28..47
                     /label=T7
                     /note="color: #00ffff; direction: RIGHT"
     misc_feature    28..44
                     /label=T7 Promoter
                     /note="color: #00ff00"
     misc_binding    47..69
                     /label=LacO
                     /note="color: #6495ed"
     misc_feature    95..107
                     /label=RBS
                     /note="color: #ff0080"
     misc_feature    116..118
                     /label=GFP-ssra
                     /note="color: #80ff80"
     CDS             119..1168
                     /codon_start=1
                     /label=QF
                     /note="color: #800080"
                     /translation="PPKRKTLNAAAEANAHADGHADGNADGHVANTAASSNNARFADLT
                     NIDTPGLGPTTTTLLVEPARSKRQRVSRACDQCRAAREKCDGIQPACFPCVSQGRSCTY
                     QASPKKRGVQTGYIRTLELALAWMFENVARSEDALHNLLVRDAGQGSALLVGKDSPAAE
                     RLHARWATSRVNKSITRLLRQLELPPTATATASIMPHVMEQPLSTSINPVNDRFNGIPN
                     PTPYNSDAALDAITQTNDYGSVNTHGILSTYPPPATHLNEASVALAPGGAPPRPPPPYV
                     DSTTNHPPYHSNLVPMANFGYSTVDYDAMVDDLASIEYTDAVDVDPQFMTNLGFVPGCN
                     FSDINTYEQ"
     CDS             1092..1168
                     /codon_start=1
                     /label=QF
                     /note="color: #800080"
                     /translation="DPQFMTNLGFVPGCNFSDINTYEQ"
     primer_bind     1202..1220
                     /label=T7
                     /note="color: #00ffff; direction: RIGHT"
     misc_feature    1202..1218
                     /label=T7 Promoter
                     /note="color: #00ff00"
     protein_bind    1221..1239
                     /gene="tetO"
                     /allele="Tet operator (TetO2 from Tn10). TetR binds here to
                     suppress transcription."
                     /label=TetO
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     misc_feature    1269..1281
                     /label=RBS
                     /note="color: #ff0080"
     CDS             1290..1946
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /note="color: #993366"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSGSREFRSY"
     misc_feature    1953..2000
                     /label=T7 stop
                     /note="color: #800000"
     misc_feature    2009..2921
                     /label=p15A Origin
                     /note="color: #8080ff"
     misc_feature    2928..3022
                     /label=T0
                     /note="color: #ff8000"
     CDS             complement(3053..3844)
                     /label=Kan/neoR
                     /note="color: #ffff00; direction: LEFT"
ORIGIN
        1 gaattccgct agccgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg
       61 ataacaattc ccctctagaa ataattttgt ttaactttaa gaaggaggag tacatatgcc
      121 acccaagcgc aaaacgctta acgctgcggc tgaggctaac gctcatgccg acggacacgc
      181 cgacggaaac gccgacggac acgtggccaa tacggccgcg tcctcgaata atgcgaggtt
      241 cgctgatctc actaacatcg atactccggg tctgggaccc acaactacga ccctgctcgt
      301 ggaaccagca cgctcaaagc gtcaacgagt gtcccgcgca tgcgaccagt gccgtgcagc
      361 ccgagagaaa tgcgacggaa tacagcctgc gtgtttcccg tgcgtttccc agggaaggtc
      421 ctgcacttat caggcttcgc cgaaaaagag gggagttcaa accggttata ttcgtacgct
      481 ggagctcgcc ctcgcctgga tgtttgaaaa tgtcgcgcgt tccgaagatg ccttgcataa
      541 cctcctcgtc cgtgacgccg gacaaggatc agctctgctc gttggtaaag attcgccggc
      601 tgccgagcga ctccatgccc gttgggctac tagccgtgtc aataagagca ttacccgcct
      661 cctccgtcag ttggagctcc ctcctaccgc cacggctacg gcctcgataa tgccgcacgt
      721 gatggagcag cctctcagta ccagcattaa ccccgtcaac gaccgcttca acggtattcc
      781 caaccccact ccgtataact ccgatgcagc tctcgatgct atcactcaga ccaacgatta
      841 tggaagcgta aatacacatg gtatcctctc tacttacccg ccaccggcta cgcaccttaa
      901 tgaagcttcc gtcgctctcg ctcccggtgg cgcccccccc cgaccgcctc ctccgtatgt
      961 tgacagcacg accaatcacc cgccgtacca ctcgaatctg gttccaatgg cgaactttgg
     1021 ttactcgacc gttgattacg atgccatggt tgacgatttg gctagcattg aatacacgga
     1081 cgctgtggat gtcgacccac agtttatgac caatctggga ttcgttcctg gatgtaactt
     1141 ctccgacatt aatacatacg aacagtgata taaccccggt tgctgaggga atagtctcga
     1201 gtaatacgac tcactatagg tccctatcag tgatagagat gcgcaagcct agaaataatt
     1261 ttgtttaact ttaagaagga ggaggatcca tgtctagatt agataaaagt aaagtgatta
     1321 acagcgcatt agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg
     1381 cccagaagct aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt
     1441 tgctcgacgc cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag
     1501 aaggggaaag ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac
     1561 taagtcatcg cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg
     1621 aaactctcga aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat
     1681 tatatgcact cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc
     1741 atcaagtcgc taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac
     1801 gacaagctat cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg
     1861 aattgatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc gcgtacagcg
     1921 gatcccggga attcagatct tattaaaagc ttctagcata accccttggg gcctctaaac
     1981 gggtcttgag gggttttttg gcggccgcac aacttatatc gtatggggct gacttcaggt
     2041 gctacatttg aagagataaa ttgcactgaa atctagaaat attttatctg attaataaga
     2101 tgatcttctt gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc
     2161 gccttgcagg gcggtttttc gaaggttctc tgagctacca actctttgaa ccgaggtaac
     2221 tggcttggag gagcgcagtc accaaaactt gtcctttcag tttagcctta accggcgcat
     2281 gacttcaaga ctaactcctc taaatcaatt accagtggct gctgccagtg gtgcttttgc
     2341 atgtctttcc gggttggact caagacgata gttaccggat aaggcgcagc ggtcggactg
     2401 aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt
     2461 caggcgtgga atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg
     2521 caggaacagg agagcgcacg agggagccgc cagggggaaa cgcctggtat ctttatagtc
     2581 ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc gtgatgcttg tcaggggggc
     2641 ggagcctatg gaaaaacggc tttgccgcgg ccctctcact tccctgttaa gtatcttcct
     2701 ggcatcttcc aggaaatctc cgccccgttc gtaagccatt tccgctcgcc gcagtcgaac
     2761 gaccgagcgt agcgagtcag tgagcgagga agcggaatat atcctgtatc acatattctg
     2821 ctgacgcacc ggtgcagcct tttttctcct gccacatgaa gcacttcact gacaccctca
     2881 tcagtgccaa catagtaagc cagtatacac tccgctagcg cactagtatt ctcaccaata
     2941 aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt
     3001 actggatcta tcaacaggag tccaagcgag ctctcgaacc ccagagtccc gctcagaaga
     3061 actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa
     3121 gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca
     3181 acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa
     3241 agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat
     3301 cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct
     3361 gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc
     3421 gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca
     3481 gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca
     3541 ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa
     3601 cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct
     3661 cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc
     3721 cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt
     3781 catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt
     3841 caatcatgcg aaacgatcct catcctgtct cttgatcaga tcttgatccc ctgcgccatc
     3901 agatccttgg cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag
     3961 agggcgcccc agctggcaat tccgacgtc
//
