LOCUS       Exported               15360 bp ds-DNA     circular SYN 15-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...5f-78jzrzb
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 17, 2021 from SnapGene Viewer 5.2.5
            https://www.snapgene.com
COMMENT     SerialCloner_Ends=0,0,,0,.
FEATURES             Location/Qualifiers
     source          1..15360
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          13405..15108
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="/Transferred_From=【DEST】pK7WG2DIPS_RPS5Apro - 
                     コピー.xdna, pMDCDIPS20181026作製途中ほぼ完成？"
                     /note="/Transferred_Similarity=100.00% -> 99.65%"
                     /note="/X-Transferred_From=pMDCDIPS20181026作製途中ほぼ完成？"
                     /note="/X-Transferred_Similarity=99.65%"
     primer_bind     7..41
                     /label=pOLEFw into KpnI
     misc_feature    22..3431
                     /label=GTAC
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=GTAC"
     misc_feature    22..1469
                     /label=pOLE1
                     /note="Geneious type: promoter uukaryotic"
     primer_bind     22..41
                     /label=pOLEFw
     CDS             1470..2228
                     /label=OLE1
     misc_feature    complement(2249..2269)
                     /label=nonstandard type: attB2
     primer_bind     2268..2302
                     /label=Ole+GFPstart
     CDS             2283..2999
                     /label=Egfp
     primer_bind     2283..2302
                     /label=GFP_start
     terminator      complement(3194..3419)
                     /label=t35S
     primer_bind     complement(3412..3446)
                     /label=35STerRev in KpnI 2
     primer_bind     complement(3412..3431)
                     /label=35sTerRv
     misc_feature    3433..3551
                     /label=CTCGAATTATCATACATGAGAATTAAGGGAGTCACGTTATGACCCCC...
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=CTCGAATTATCATACATGAGAATTAAGGGAGTCACG
                     TTATG 
                     ACCCCCGCCGATGACGCGGGACAAGCCGTTTTACGTTTGGAACTGACAGAACCGCAAC 
                     GTTGAAGGAGCCACTC"
     misc_feature    3457..4844
                     /label=Kan_gent
     promoter        3457..3726
                     /label=pNOS(pB7WG_ASX)
                     /note="/created_by=Issei.N"
     primer_bind     complement(3468..3487)
                     /label=pK7WG2_fsRv
     regulatory      3526..3705
                     /regulatory_class="promoter"
                     /label=nopaline synthase (nos) promoter
                     /note="nopaline synthase (nos) promoter"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotatefrom 
                     Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     nopalinesynthase (nos) promoter regulatory (<a 
                     href=""urn:local:.:21q-c8b5k3j"">MN379653</a>)"
                     /note="/Transferred_From=MN379653"
                     /note="/Transferred_Similarity=100.00%"
     CDS             3739..4533
                     /codon_start=1
                     /transl_table=11
                     /gene="nptII"
                     /product="neomycin phosphotransferase"
                     /label=nptII
                     /note="plant selection marker gene 
                     (conferskanamycin/neomycin resistance)"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotatefrom 
                     Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     nptIICDS (<a 
                     href=""urn:local:.:21q-c8b5k3j"">MN379653</a>)"
                     /note="/Transferred_From=MN379653"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/Transferred_Translation=MIEQDGLHAGSPAAWVERLFGYDWAQQ
                     TIGCS 
                     DAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRD 
                     WLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRM 
                     EAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGF 
                     IDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF 
                     "
                     /db_xref="GI:1820247507"
                     /protein_id="QIJ31329.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     4108..4127
                     /label=Kanamycin_qFw
     primer_bind     complement(4206..4225)
                     /label=Kanamycin_fsRv
     misc_feature    4848..5180
                     /label=LB_gent
     primer_bind     complement(4986..5005)
                     /label=LB_fsRv
     misc_feature    5186..6435
                     /label=Spm_gent
     misc_feature    5329..6261
                     /label=ORF 1 (frame 2)
                     /label=nonstandard type: ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=2"
                     /note="/Transferred_From=pMDCDIPS20181026作製途中ほぼ完成？,【DEST】p
                     K7WG2DIPS_RPS5Apro - コピー.xdna"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=[亘完成]pDESTpK7WG2DIPS_RPS5A_cTAL1
                     2; pMDCDIPS20181026作製途中ほぼ完成？; 
                     【完成】pK7WG2DIPSHSP_platinumORF125_1.xdna Copy"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=ORF Finder"
     primer_bind     complement(5425..5444)
                     /label=Spm_fsRv
     misc_feature    6479..7152
                     /label=pUC origin
                     /note="Geneious type: Origin of replication"
                     /note="/Transferred_From=pMDCDIPS20181026作製途中ほぼ完成？,【DEST】p
                     K7WG2DIPS_RPS5Apro - コピー.xdna, DP1 GFP vector confirmed"
                     /note="/Transferred_Similarity=100.00% -> 99.85%"
                     /note="/X-Transferred_From=DP1 GFP vector confirmed; 
                     pCRII-TOPO; pMDCDIPS20181026作製途中ほぼ完成？; Virtual DIPS GFP 
                     sakamoto transient plasmid; 
                     [construction予定]L1-L4pENTRATP62upLeft MSH1; 
                     [亘完成]pDESTpK7WG2DIPS_RPS5A_cTAL12; 
                     [反対完成E1]pENTR_L1-L4_ATP621A=cTAL12; 
                     [反対完成E3]pENTR_L3-L2ATP621uprightB.xdna; [完成Tandem] 
                     RPS5A-ATP6-2up 20180424.xdna; 
                     [完成]cTAL12pENTRL1-L2-20180423.xdna; [完成]pENTRMSH1 virtual 
                     SA20180709.xdna; [完成]鈴置L1-L4pENTRATP62upLeft; 
                     [完成]鈴置L3-L2pENTRATP62upRight; [間違え完成Tandem] 
                     RPS5A-ATP6-2-1up 20180423.xdna; pENTR221; 【E1】pENTR L1-L4 
                     cTAL12virtual 20180423.xdna; 【E3】pENTR L3-L2 wakoTAL-R 
                     ATP621upRight.xdna Copy; 
                     【完成】pK7WG2DIPSHSP_platinumORF125_1.xdna Copy; 
                     【柳瀬間違え】RPS5A_platinum　ATP6-2 ①.xdna; 鈴置L1-L4; 鈴置L3-L2"
                     /note="/X-Transferred_Similarity=100.00% -> 99.85%; 
                     99.85%;100.00% -> 99.55%"
     rep_origin      6483..7111
                     /direction=RIGHT
                     /label=ColE1 origin
     misc_feature    complement(8023..9147)
                     /label=ORF 5 (frame 1)
                     /label=nonstandard type: ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=1"
                     /note="/Transferred_From=pMDCDIPS20181026作製途中ほぼ完成？,【DEST】p
                     K7WG2DIPS_RPS5Apro - コピー.xdna"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=[亘完成]pDESTpK7WG2DIPS_RPS5A_cTAL1
                     2; pMDCDIPS20181026作製途中ほぼ完成？; 
                     【完成】pK7WG2DIPSHSP_platinumORF125_1.xdna Copy"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=ORF Finder"
     misc_feature    9304..9315
                     /label=nonstandard type: helix
                     /note="/Transferred_From=pMDCDIPS20181026作製途中ほぼ完成？,【DEST】p
                     K7WG2DIPS_RPS5Apro - コピー.xdna"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=NARH; 
                     pMDCDIPS20181026作製途中ほぼ完成？"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/X-Transferred_Translation_Similarity=100.00%"
     misc_feature    complement(10850..10861)
                     /label=nonstandard type: helix
                     /note="/Transferred_From=pMDCDIPS20181026作製途中ほぼ完成？,【DEST】p
                     K7WG2DIPS_RPS5Apro - コピー.xdna"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=NARH; 
                     pMDCDIPS20181026作製途中ほぼ完成？"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/X-Transferred_Translation_Similarity=100.00%"
     primer_bind     11100..11119
                     /label=RB_fsFw
     misc_feature    11339..13529
                     /label=sequenced
                     /label=nonstandard type: motif
                     /note="/created_by=first"
     misc_feature    11339..11569
                     /label=RB(pK2GW7)
                     /note="/created_by=Issei.N"
                     /note="/modified_by=Issei.N"
     misc_feature    11342..11454
                     /label=GATCCCGAGGGGAACCCTGTGGTTGGCATGCACATACAAATGGACGA...
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=GATCCCGAGGGGAACCCTGTGGTTGGCATGCACATA
                     CAAAT GGACGAACGGATAAACCTTTTCACGCCCTTTTAAATATCCG"
     misc_feature    11570..13229
                     /label=RPS5Apro
     primer_bind     12162..12181
                     /label=pRPS5A_fsFw1
     primer_bind     12952..12971
                     /label=pRPS5A_fsFw2
     misc_feature    13230..13391
                     /label=CTP
                     /label=nonstandard type: motif
                     /note="/created_by=first"
     primer_bind     13230..13254
                     /label=CTP_Fw
     misc_feature    13233..13391
                     /label=TTTAAACAAGCTTCTCGTCTCCTCTCCCGATCTGTCGCCGCCGCATC...
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=TTTAAACAAGCTTCTCGTCTCCTCTCCCGATCTGTC
                     GCCGC 
                     CGCATCTTCCAAATCGGTGACGACTCGTGCCTTTTCAACGGAACTTCCATCGACGCTC 
                     GATTCC"
     primer_bind     complement(13371..13391)
                     /label=CTP_Rv
     misc_feature    13405..13529
                     /label=nonstandard type: attR1
     promoter        13553..13583
                     /label=lac UV5 promoter; lac promoter
                     /note="E. coli lac promoter with an ""up"" 
                     mutation;promoter for the E. coli lac operon"
                     /note="/Transferred_From=Exported, 
                     pMDCDIPS20181026作製途中ほぼ完成？, 【DEST】pK7WG2DIPS_RPS5Apro - 
                     コピー.xdna, Exported"
                     /note="/Transferred_Similarity=100.00% -> 90.32%"
                     /note="/X-Transferred_From=Exported; 
                     pMDCDIPS20181026作製途中ほぼ完成？"
                     /note="/X-Transferred_Similarity=100.00%"
     promoter        13553..13582
                     /label=lac
                     /note="/Transferred_From=DP1 GFP vector confirmed"
                     /note="/Transferred_Similarity=93.33%"
                     /note="/X-Transferred_From=?E1?pENTR_L1-L4_;?E1?pENTR_L1-L4
                     _wako_136_63-VR-HD.xdna; 
                     ?E3?pENTR_L3-L2_wako_136_63-VR-NG.txt.xdna; Virtual DIPS 
                     GFP sakamoto transient plasmid"
                     /note="/X-Transferred_Similarity=100.00%"
     gene            13637..14296
                     /gene="cat"
                     /label=cat
                     /note="/Transferred_From=pMDCDIPS20181026作製途中ほぼ完成？,【DEST】p
                     K7WG2DIPS_RPS5Apro - コピー.xdna"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pBeloBAC11; pFOS1; pGPS2.1; 
                     pGPS4; pMDCDIPS20181026作製途中ほぼ完成？"
                     /note="/X-Transferred_Similarity=100.00%; 100.00% -> 
                     99.85%"
     CDS             13637..14296
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /note="/Transferred_From=Exported, 
                     【DEST】pK7WG2DIPS_RPS5Apro -コピー.xdna"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/Transferred_Translation=MEKKITGYTTVDISQWHRKEHFEAFQS
                     VAQCT 
                     YNQTVQLDITAFLKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHP 
                     CYTVFHEQTETFSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSAN 
                     PWVSFTSFDLNVANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLN 
                     ELQQYCDEWQGGA"
                     /note="/X-Transferred_From=Exported"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/X-Transferred_Translation=MEKKITGYTTVDISQWHRKEHFEAF
                     QSVAQ 
                     CTYNQTVQLDITAFLKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSV 
                     HPCYTVFHEQTETFSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVS 
                     ANPWVSFTSFDLNVANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRM 
                     LNELQQYCDEWQGGA"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             13637..14293
                     /label=CmR
     primer_bind     complement(13703..13722)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
                     /note="/Transferred_From=【DEST】pK7WG2DIPS_RPS5Apro - 
                     コピー.xdna"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=Exported"
                     /note="/X-Transferred_Similarity=100.00%"
     primer_bind     complement(13703..13722)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
                     /note="/Transferred_From=Exported"
                     /note="/Transferred_Similarity=100.00%"
     gene            14638..14943
                     /label=ccdB
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=pMDCDIPS20181026作製途中ほぼ完成？,【DEST】p
                     K7WG2DIPS_RPS5Apro - コピー.xdna"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=ccdB; 
                     pMDCDIPS20181026作製途中ほぼ完成？"
                     /note="/X-Transferred_Similarity=100.00%"
     CDS             14638..14943
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot 
                     bepropagated in standard E. coli strains."
                     /note="/Transferred_From=Exported, 
                     【DEST】pK7WG2DIPS_RPS5Apro -コピー.xdna"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/Transferred_Translation=MQFKVYTYKRESRYRLFVDVQSDIIDT
                     PGRRM 
                     VIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDI 
                     KNAINLMFWGI"
                     /note="/X-Transferred_From=Exported"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/X-Transferred_Translation=MQFKVYTYKRESRYRLFVDVQSDII
                     DTPGR 
                     RMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHREN 
                     DIKNAINLMFWGI"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             14731..14940
                     /label=ccdB
     primer_bind     14822..14841
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
                     /note="/Transferred_From=Exported"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     14822..14841
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
                     /note="/Transferred_From=【DEST】pK7WG2DIPS_RPS5Apro - 
                     コピー.xdna"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=Exported"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    complement(14983..15107)
                     /label=nonstandard type: attR2
     misc_feature    15118..15343
                     /label=ter35s
     primer_bind     complement(15336..15357)
                     /label=35STerRev in KpnI 2
     primer_bind     complement(15336..15355)
                     /label=35sTerRv
ORIGIN
        1 ggccctctag aggatccccg gcaccctact tagatcaaca cataaaagtt agtaagtgaa
       61 gaaccacaac aacaacacta gattcatctt caagtgtatg taggtatagt aacatgaaca
      121 agaacagact caagtacaag atcgcatacg aaaatggaaa tggcaatgtc acttccacat
      181 aatcaaacac gaatcctcat atcaacaagg cctgagattc taactagctc ataacaactt
      241 agccaatagt tacttgagac taccaaatgt atgtagaact aaagactaag ggacagagag
      301 ttcgtctaaa caggtgaatc tagtcgttgt tatctaataa acaattcagc cccaaatgca
      361 gaacacacat agagctctct attgattcaa attacgatct gatactgata acgtctagat
      421 ttttagggtt aaagcaatca atcacctgac gattcaaggt ggttggatca tgacgattcc
      481 agaaaacatc aagcaagctc tcaaagctac actctttggg atcatactga actctaacaa
      541 cctcgttatg tcccgtagtg ccagtacaga catcctcgta actcggattg tgcacgatgc
      601 catggctata cccaacctcg gtcttggtca caccaggaac tctctggtaa gctagctcca
      661 ctccccagaa acaaccggcg ccaaattgcg cgaattgctg acctgaagac ggaacatcat
      721 cgtcgggtcc ttgggcgatt gcggcggaag atgggtcagc ttgggcttga ggacgagacc
      781 cgaatccgag tctgttgaaa aggttgttca ttggggattt gtatacggag attggtcgtc
      841 gagaggtttg agggaaagga caaatgggtt tggctctgga gaaagagagt gcggctttag
      901 agagagaatt gagaggttta gagagagatg cggcggcgat gagcggagga gagacgacga
      961 ggacctgcat tatcaaagca gtgacgtggt gaaatttgga acttttaaga ggcagataga
     1021 tttattattt gtatccattt tcttcattgt tctagaatgt cgcggaacaa attttaaaac
     1081 taaatcctaa atttttctaa ttttgttgcc aatagtggat atgtgggccg tatagaagga
     1141 atctattgaa ggcccaaacc catactgacg agcccaaagg ttcgttttgc gttttatgtt
     1201 tcggttcgat gccaacgcca cattctgagc taggcaaaaa acaaacgtgt ctttgaatag
     1261 actcctctcg ttaacacatg cagcggctgc atggtgacgc cattaacacg tggcctacaa
     1321 ttgcatgatg tctccattga cacgtgactt ctcgtctcct ttcttaatat atctaacaaa
     1381 cactcctacc tcttccaaaa tatatacaca tctttttgat caatctctca ttcaaaatct
     1441 cattctctct agtaaacaag aacaaaaaaa tggcggatac agctagagga acccatcacg
     1501 atatcatcgg cagagaccag tacccgatga tgggccgaga ccgagaccag taccagatgt
     1561 ccggacgagg atctgactac tccaagtcta ggcagattgc taaagctgca actgctgtca
     1621 cagctggtgg ttccctcctt gttctctcca gccttaccct tgttggaact gtcatagctt
     1681 tgactgttgc aacacctctg ctcgttatct tcagcccaat ccttgtcccg gctctcatca
     1741 cagttgcact cctcatcacc ggttttcttt cctctggagg gtttggcatt gccgctataa
     1801 ccgttttctc ttggatttac aagtaagcac acatttatca tcttacttca taattttgtg
     1861 caatatgtgc atgcatgtgt tgagccagta gctttggatc aatttttttg gtcgaataac
     1921 aaatgtaaca ataagaaatt gcaaattcta gggaacattt ggttaactaa atacgaaatt
     1981 tgacctagct agcttgaatg tgtctgtgta tatcatctat ataggtaaaa tgcttggtat
     2041 gatacctatt gattgtgaat aggtacgcaa cgggagagca cccacaggga tcagacaagt
     2101 tggacagtgc aaggatgaag ttgggaagca aagctcagga tctgaaagac agagctcagt
     2161 actacggaca gcaacatact ggtggggaac atgaccgtga ccgtactcgt ggtggccagc
     2221 acactactca agggtgggcg cgccgaccca gctttcttgt acaaagtggt gatatcccgc
     2281 ggatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     2341 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     2401 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     2461 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     2521 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     2581 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     2641 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     2701 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     2761 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     2821 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     2881 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     2941 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacgaac
     3001 ccggcatgtt acgtcctgta gaaaccccaa cccgtgaaat caaaaaactc gacggcctgt
     3061 gggcattcag tctggatcgc gaccgcaaac cgaagtcggc ggcttttctg ctgcaaaaac
     3121 gctggactgg catgaacttc ggtgaaaaac cgcagcaggg aggcaaacaa tgaccatggc
     3181 ggccgggagc atgcggccat gctagagtcc gcaaaaatca ccagtctctc tctacaaatc
     3241 tatctctctc tatttttctc cagaataatg tgtgagtagt tcccagataa gggaattagg
     3301 gttcttatag ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta
     3361 tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc cagtgacctg
     3421 caggcatgcg acgcgaatta tcgatcatga gcggagaatt aagggagtca cgttatgacc
     3481 cccgccgatg acgcgggaca agccgtttta cgtttggaac tgacagaacc gcaacgttga
     3541 aggagccact gagccgcggg tttctggagt ttaatgagct aagcacatac gtcagaaacc
     3601 attattgcgc gttcaaaagt cgcctaaggt cactatcagc tagcaaatat ttcttgtcaa
     3661 aaatgctcca ctgacgttcc ataaattccc ctcggtatcc aattagagtc tcatattcac
     3721 tctcaactcg atcgaggcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     3781 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     3841 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     3901 ggtgccctga atgaactcca agacgaggca gcgcggctat cgtggctggc cacgacgggc
     3961 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     4021 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     4081 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     4141 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     4201 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     4261 aaggcgcgga tgcccgacgg cgaggatctc gtcgtgaccc acggcgatgc ctgcttgccg
     4321 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     4381 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     4441 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     4501 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc ggactctagc
     4561 tagagtcaag cagatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
     4621 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
     4681 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
     4741 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
     4801 gcggtgtcat ctatgttact agatcgaccg gcatgcaagc tgataattca attcggcgtt
     4861 aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt
     4921 tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac
     4981 aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt
     5041 tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct
     5101 gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag
     5161 agcgttgctg cctgtgatca attcgggcac gaacccagtg gacataagcc tcgttcggtt
     5221 cgtaagctgt aatgcaagta gcgtaactgc cgtcacgcaa ctggtccaga accttgaccg
     5281 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttcttgttat gacatgtttt
     5341 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
     5401 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
     5461 ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
     5521 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
     5581 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg
     5641 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
     5701 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
     5761 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
     5821 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
     5881 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
     5941 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
     6001 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
     6061 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
     6121 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
     6181 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc
     6241 accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac gccgcttcgc
     6301 cggcgttaac tcaagcgatt agatgcacta agcacataat tgctcacagc caaactatca
     6361 ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga
     6421 tatatcatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6481 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6541 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6601 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     6661 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6721 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6781 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6841 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6901 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6961 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7021 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7081 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7141 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     7201 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     7261 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     7321 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     7381 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     7441 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     7501 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     7561 ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg
     7621 tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg
     7681 cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc
     7741 ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag
     7801 ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat
     7861 gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat
     7921 gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt ttcgaccttt
     7981 ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc
     8041 ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc
     8101 ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca
     8161 gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct
     8221 ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg
     8281 atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat
     8341 ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct
     8401 ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc
     8461 gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt
     8521 ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc
     8581 cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt
     8641 catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc
     8701 catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac
     8761 ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg
     8821 actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc
     8881 cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg
     8941 gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg
     9001 ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc
     9061 gatttgtacc gggccggatg gtttgcgacc gtcacgccga ttcctcgggc ttgggggttc
     9121 cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa ccgcccgttc
     9181 ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc
     9241 ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc
     9301 tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc
     9361 ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg
     9421 tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt
     9481 agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt
     9541 taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa
     9601 gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact
     9661 caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc
     9721 ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc
     9781 gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca
     9841 ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg
     9901 gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg
     9961 tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc
    10021 gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg
    10081 cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag
    10141 cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc
    10201 agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg
    10261 ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc
    10321 ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc
    10381 ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc
    10441 ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg
    10501 gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt gggcgtcact
    10561 tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc
    10621 gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc
    10681 ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg
    10741 ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc gaacacggtc
    10801 aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag gctacgcagg
    10861 cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc
    10921 aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg
    10981 gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag cttggtgcag
    11041 ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg
    11101 gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag
    11161 tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc
    11221 agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc agaagacggc
    11281 tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat caaagtactt
    11341 taaagtactt taaagtactt taaagtactt tgatcccgag gggaaccctg tggttggcat
    11401 gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat ccgattattc
    11461 taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt
    11521 ttaaactgaa ggcgggaaac gacaatctga tccaagctca agctaagctc tcaacttttg
    11581 attcgctatt tgcagtgcac ctgtggcgtt catcacatct tttgtgacac tgtttgcact
    11641 ggtcattgct attacaaagg accttcctga tgttgaagga gatcgaaagt aagtaactgc
    11701 acgcataacc attttctttc cgctctttgg ctcaatccat ttgacagtca aagacaatgt
    11761 ttaaccagct ccgtttgata tattgtcttt atgtgtttgt tcaagcatgt ttagttaatc
    11821 atgcctttga ttgatcttga ataggttcca aatatcaacc ctggcaacaa aacttggagt
    11881 gagaaacatt gcattcctcg gttctggact tctgctagta aattatgttt cagccatatc
    11941 actagctttc tacatgcctc aggtgaattc atctatttcc gtcttaacta tttcggttaa
    12001 ttaaagcacg aacaccatta ctgcatgtag aagcttgata aactatcgcc accaatttat
    12061 ttttgttgcg atattgttac tttcctcagt atgcagcttt gaaaagacca accctcttat
    12121 cctttaacaa tgaacaggtt tttagaggta gcttgatgat tcctgcacat gtgatcttgg
    12181 cttcaggctt aattttccag gtaaagcatt atgagatact cttatatctc ttacatactt
    12241 ttgagataat gcacaagaac ttcataacta tatgctttag tttctgcatt tgacactgcc
    12301 aaattcatta atctctaata tctttgttgt tgatctttgg tagacatggg tactagaaaa
    12361 agcaaactac accaaggtaa aatacttttg tacaaacata aactcgttat cacggaacat
    12421 caatggagtg tatatctaac ggagtgtaga aacatttgat tattgcagga agctatctca
    12481 ggatattatc ggtttatatg gaatctcttc tacgcagagt atctgttatt ccccttcctc
    12541 tagctttcaa tttcatggtg aggatatgca gttttctttg tatatcattc ttcttcttct
    12601 ttgtagcttg gagtcaaaat cggttccttc atgtacatac atcaaggata tgtccttctg
    12661 aatttttata tcttgcaata aaaatgcttg taccaattga aacaccagct ttttgagttc
    12721 tatgatcact gacttggttc taaccaaaaa aaaaaaaatg tttaatttac atatctaaaa
    12781 gtaggtttag ggaaacctaa acagtaaaat atttgtatat tattcgaatt tcactcatca
    12841 taaaaactta aattgcacca taaaattttg ttttactatt aatgatgtaa tttgtgtaac
    12901 ttaagataaa aataatattc cgtaagttaa ccggctaaaa ccacgtataa accagggaac
    12961 ctgttaaacc ggttctttac tggataaaga aatgaaagcc catgtagaca gctccattag
    13021 agcccaaacc ctaaatttct catctatata aaaggagtga cattagggtt tttgttcgtc
    13081 ctcttaaagc ttctcgtttt ctctgccgtc tctctcattc gcgcgacgca aacgatcttc
    13141 aggtgatctt ctttctccaa atcctctctc ataactctga tttcgtactt gtgtatttga
    13201 gctcacgctc tgtttctctc accacagcca tggattcaca gctagtcttg tctctgaagc
    13261 tgaatccaag cttcactcct ctttctcctc tcttcccttt cactccatgt tcttcttttt
    13321 cgccgtcgct ccggttttct tcttgctact cccgccgcct ctattctccg gttaccgtct
    13381 acgccgcgaa ggctagtgat atcacaagtt tgtacaaaaa agctgaacga gaaacgtaaa
    13441 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
    13501 gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact
    13561 ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccggcga gattttcagg
    13621 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
    13681 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
    13741 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
    13801 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat
    13861 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
    13921 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
    13981 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
    14041 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
    14101 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
    14161 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
    14221 ctgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
    14281 gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa gccagataac agtatgcgta
    14341 tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc
    14401 aaaaagaggt gtgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag
    14461 ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg
    14521 aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg
    14581 tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacagggac tggtgaaatg
    14641 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag
    14701 agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg
    14761 ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg
    14821 cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct
    14881 gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata
    14941 taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg
    15001 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat
    15061 atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggtga tatcccgcgg
    15121 ccatgctaga gtccgcaaaa atcaccagtc tctctctaca aatctatctc tctctatttt
    15181 tctccagaat aatgtgtgag tagttcccag ataagggaat tagggttctt atagggtttc
    15241 gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct
    15301 atcaataaaa tttctaattc ctaaaaccaa aatccagtga cctgcaggca tgcgacgtcg
//
