LOCUS       Exported                4052 bp ds-DNA     circular SYN 15-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...j-7twiatv
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 17, 2021 from SnapGene Viewer 5.2.5
            https://www.snapgene.com
COMMENT     SerialCloner_Ends=0,0,,0,.
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..57
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    1..57
                     /label=lastNI
                     /note="/created_by=User"
     misc_feature    58..180
                     /label=C-term region_Mok2020
                     /note="/modified_by=Issei.N"
     misc_feature    181..186
                     /label=2aa linker
                     /note="/created_by=Issei.N"
     misc_feature    182..579
                     /label=ATCCTATCAGCCGTTCCCAGCTGGTGAAGTCCGAGCTGGAGGAGAAG...
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=ATCCTATCAGCCGTTCCCAGCTGGTGAAGTCCGAGC
                     TGGAG 
                     GAGAAGAAATCCGAGTTGAGGCACAAGCTGAAGTACGTGCCCCACGAGTACATCGAGC 
                     TGATCGAGATCGCCCGGAACAGCACCCAGGACCGTATCCTGGAGATGAAGGTGATGGA 
                     GTTCTTCATGAAGGTGTACGGCTACAGGGGCAAGCACCTGGGCGGCTCCAGGAAGCCC 
                     GACGGCGCCATCTACACCGTGGGCTCCCCCATCGACTACGGCGTGATCGTGGACACCA 
                     AGGCCTACTCCGGCGGCTACAACCTGCCCATCGGCCAGGCCGACGAAATGCAGAGGTA 
                     CGTGGAGGAGAACCAGACCAGGAACAAGCACATCAACCCCAACGAGTGGTGGAAGGTG 
                     TACCCCTCCAGCGTGACCGAGTTCAAGTTCCTGTTCGTGTCCGGCCACTTCAAGGGCA 
                     ACTACAAGGCCCAGCTGACCAGGCTGAACCACATCACCAACTGCAACGGCGCCGTGCT 
                     GTCCGTGGAGGAGCTCCTGATCGGCGGCGAGATGATCAAGGCCGGCACCCTGACCCTG 
                     GAGGAGGTGAGGAGGAAGTTCAACAACGGCGAGATCAACTTCGCGGCCGAC"
     misc_feature    187..318
                     /label=G1333-DddtoxA-N
                     /note="/created_by=Issei.N"
                     /note="/modified_by=Issei.N"
     misc_feature    319..330
                     /label=4aa linker
                     /note="/created_by=Issei.N"
     misc_feature    331..579
                     /label=UGI
                     /note="/created_by=Issei.N"
     misc_recomb     complement(605..703)
                     /label=attL4
     misc_recomb     605..628
                     /label=attB4
     primer_bind     complement(728..747)
                     /label=T7
     primer_bind     complement(748..768)
                     /label=M13-rev
     CDS             881..1687
                     /label=KanR
     rep_origin      1815..2443
                     /direction=RIGHT
                     /label=ColE1 origin
     terminator      complement(2755..2782)
                     /label=rrnB T2
     terminator      complement(2914..2957)
                     /label=rrnB T1
     primer_bind     3023..3040
                     /label=M13-fwd
     misc_recomb     3057..3152
                     /label=AttL1
     CDS             3158..3184
                     /label=HA tag
     misc_feature    3191..3598
                     /label=N-term region_wako; N-term region_wako compact;...
                     /note="/Transferred_From=【E1】pENTR_L1-L4_NI_G1397-DddtoxA-C
                     , 【E1】pENTR_L1-L4_NI_G1397-DddtoxA-N, 
                     【E1】pENTR_L1-L4_NN_G1333-DddtoxA-C, 
                     【E1】pENTR_L1-L4_NN_G1333-DddtoxA-N, 
                     【E1】pENTR_L1-L4_NN_G1397-DddtoxA-C, 
                     【E1】pENTR_L1-L4_NN_G1397-DddtoxA-N, 
                     pK7WG2DIPSHSP_ORF125_1.xdna, pK7WG2DIPSHSP_ORF125_2.xdna, 
                     pH7WG2DIPS_ORF79cTAL14.xdna, pH7WG2DIPS_ORF79cTAL8.xdna"
                     /note="/Transferred_Similarity=100.00% -> 99.26%"
                     /note="/X-Transferred_From=pH7WG2DIPS_ORF79cTAL14.xdna;pH7W
                     G2DIPS_ORF79cTAL8.xdna; pK7WG2DIPSHSP_ORF125_1.xdna; 
                     pK7WG2DIPSHSP_ORF125_2.xdna; 
                     【E1】pENTR_L1-L4_NI_G1397-DddtoxA-N; 
                     【E1】pENTR_L1-L4_NN_G1333-DddtoxA-C; 
                     【E1】pENTR_L1-L4_NN_G1333-DddtoxA-N; 
                     【E1】pENTR_L1-L4_NN_G1397-DddtoxA-C; 
                     【E1】pENTR_L1-L4_NN_G1397-DddtoxA-N"
                     /note="/X-Transferred_Similarity=100.00% -> 99.26%; 99.75% 
                     ->99.26%"
     CDS             complement(3626..3694)
                     /label=LacZ alpha
     primer_bind     3765..3782
                     /label=M13-fwd
     primer_bind     3792..3811
                     /label=T7
     primer_bind     complement(3856..3875)
                     /label=T3
     primer_bind     complement(3893..3913)
                     /label=M13-rev
     misc_binding    complement(3919..3941)
                     /label=LacO
     promoter        complement(3946..3975)
                     /label=lac
     misc_feature    join(3978..4052,1..582)
                     /label=ORF 4 (frame 3)
                     /label=nonstandard type: ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=3"
                     /note="/created_by=ORF Finder"
ORIGIN
        1 acccctgagc aggtagtggc tattgcatcc aacatagggg gcagacccgc actggagtca
       61 attgttgccc agttatctcg ccctgatccg gcgttggccg cgttgaccaa cgaccacctc
      121 gtcgccttgg cctgcctcgg cgggcgtcct gcgctggatg cagtgaaaaa gggattgggg
      181 ggatctggta gctatgcgtt aggaccctat cagatttcag ctcctcaatt gcctgcctat
      241 aatgggcaaa ctgttggcac cttttactac gtcaatgatg ctggagggtt agaatccaag
      301 gtgttctcaa gtggtggttc tggaggtagt acgaatcttt cggacatcat agagaaggaa
      361 actggaaaac agctcgttat ccaagagagc attctcatgt tgccagaaga agttgaagag
      421 gttataggca acaaaccgga atctgacatt ctggtacata ccgcttatga tgagtcaaca
      481 gatgagaacg tcatgctttt gacatctgat gcaccagaat acaaaccttg ggcacttgtg
      541 attcaggatt ccaatggtga gaacaagatc aagatgctat gataactcga gcgatcctct
      601 agacacccaa cttttctata caaagttggc attataagaa agcattgctt atcaatttgt
      661 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgcaggg
      721 cggccgcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
      781 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
      841 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
      901 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
      961 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     1021 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     1081 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     1141 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     1201 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     1261 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgcct
     1321 tgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     1381 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
     1441 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     1501 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     1561 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     1621 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     1681 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg
     1741 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     1801 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1861 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1921 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1981 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2041 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2101 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2161 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2221 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2281 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2341 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2401 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2461 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     2521 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     2581 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     2641 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     2701 gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg
     2761 ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct
     2821 gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc
     2881 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac
     2941 tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg
     3001 gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccccaaat
     3061 aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt
     3121 ttttataatg ccaactttgt acaaaaaagc aggcttttac ccatacgatg ttccagatta
     3181 cgctgatatc gccgatctac gcacgctcgg ctacagccag cagcaacagg agaagatcaa
     3241 accgaaggtt cgttcgacag tggcgcagca ccacgaggca ctggtcggcc acgggtttac
     3301 acacgcgcac atcgttgcgt taagccaaca cccggcagcg ttagggaccg tcgctgtcaa
     3361 gtatcaggac atgatcgcag cgttgccaga ggcgacacac gaagcgatcg ttggcgtcgg
     3421 caaacagtgg tccggcgcac gcgctctgga ggccttgctc acggtggcgg gagagttgag
     3481 aggtccaccg ttacagttgg acacaggcca acttctcaag attgcaaaac gtggcggcgt
     3541 gaccgcagtg gaggcagtgc atgcatggcg caatgcactg acgggtgccc cgctcaagga
     3601 gacgggcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
     3661 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     3721 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     3781 gtgagcgcgc gtaatacgac tcactatagg gcgaattggg taccgggccc cccctcgagg
     3841 ccctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
     3901 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     3961 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     4021 cgctcactgc ccgctttcca ccggtcgtct cc
//
