LOCUS       Exported                4033 bp ds-DNA     circular SYN 15-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...3i-ddcav01
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 17, 2021 from SnapGene Viewer 5.2.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    121..126
                     /label=2aa linker
                     /note="/created_by=Issei.N"
     misc_feature    122..476
                     /label=ATCCTATCAGCCGTTCCCAGCTGGTGAAGTCCGAGCTGGAGGAGAAG...
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=ATCCTATCAGCCGTTCCCAGCTGGTGAAGTCCGAGC
                     TGGAG 
                     GAGAAGAAATCCGAGTTGAGGCACAAGCTGAAGTACGTGCCCCACGAGTACATCGAGC 
                     TGATCGAGATCGCCCGGAACAGCACCCAGGACCGTATCCTGGAGATGAAGGTGATGGA 
                     GTTCTTCATGAAGGTGTACGGCTACAGGGGCAAGCACCTGGGCGGCTCCAGGAAGCCC 
                     GACGGCGCCATCTACACCGTGGGCTCCCCCATCGACTACGGCGTGATCGTGGACACCA 
                     AGGCCTACTCCGGCGGCTACAACCTGCCCATCGGCCAGGCCGACGAAATGCAGAGGTA 
                     CGTGGAGGAGAACCAGACCAGGAACAAGCACATCAACCCCAACGAGTGGTGGAAGGTG 
                     TACCCCTCCAGCGTGACCGAGTTCAAGTTCCTGTTCGTGTCCGGCCACTTCAAGGGCA 
                     ACTACAAGGCCCAGCTGACCAGGCTGAACCACATCACCAACTGCAACGGCGCCGTGCT 
                     GTCCGTGGAGGAGCTCCTGATCGGCGGCGAGATGATCAAGGCCGGCACCCTGACCCTG 
                     GAGGAGGTGAGGAGGAAGTTCAACAACGGCGAGATCAACTTCGCGGCCGA"
     misc_feature    127..216
                     /label=G1397-DddtoxA-C
                     /note="/created_by=Issei.N"
                     /note="/modified_by=Issei.N"
     misc_feature    217..228
                     /label=4aa linker
                     /note="/created_by=Issei.N"
     misc_feature    229..477
                     /label=UGI
                     /note="Derived using Geneious Prime 2020.1.2 'Annotatefrom 
                     Database' based on nucleotide similarity"
                     /note="/Alternative_Match=79.52%; Adjusted-Interval-0:0/0; 
                     UGI(<a href=""urn:local:.:5k-c662uqm"">2aa linker + 
                     G1333-DddtoxA-C + 4aa linker + 1*UGI     </a>)"
                     /note="/Primary_Match=83.13%; Adjusted-Interval-0:0/0; UGI 
                     (<a href=""urn:local:.:5t-c662uqm"">2aa linker + 
                     G1333-DddtoxA-N + 4aa linker + 1*UGI     </a>)"
                     /note="/Transferred_From=2aa linker + G1333-DddtoxA-N + 
                     4aalinker + 1*UGI     "
                     /note="/Transferred_Similarity=83.13%"
                     /note="/created_by=Issei.N"
     misc_recomb     complement(505..600)
                     /label=AttL2
     primer_bind     complement(631..650)
                     /label=T7
     primer_bind     complement(651..671)
                     /label=M13-rev
     CDS             784..1590
                     /label=KanR
     rep_origin      1718..2346
                     /direction=RIGHT
                     /label=ColE1 origin
     terminator      complement(2658..2685)
                     /label=rrnB T2
     terminator      complement(2817..2860)
                     /label=rrnB T1
     primer_bind     2926..2943
                     /label=M13-fwd
     misc_recomb     2960..3055
                     /label=attL3
     misc_recomb     complement(3035..3055)
                     /label=attB3
     CDS             3058..3102
                     /label=S-tag
     misc_feature    3112..3519
                     /label=N-term region_wako; N-term region_wako compact;...
                     /note="/Transferred_From=【E1】pENTR_L1-L4_HD_G1333-DddtoxA-C
                     , 【E1】pENTR_L1-L4_HD_G1333-DddtoxA-N, 
                     【E1】pENTR_L1-L4_HD_G1397-DddtoxA-C, 
                     【E1】pENTR_L1-L4_HD_G1397-DddtoxA-N, 
                     【E1】pENTR_L1-L4_NG_G1333-DddtoxA-C, 
                     【E1】pENTR_L1-L4_NG_G1333-DddtoxA-N, 
                     【E1】pENTR_L1-L4_NG_G1397-DddtoxA-C, 
                     【E1】pENTR_L1-L4_NG_G1397-DddtoxA-N, 
                     【E1】pENTR_L1-L4_NI_G1333-DddtoxA-C, 
                     【E1】pENTR_L1-L4_NI_G1333-DddtoxA-N, 
                     【E1】pENTR_L1-L4_NI_G1397-DddtoxA-C, 
                     【E1】pENTR_L1-L4_NI_G1397-DddtoxA-N, 
                     【E1】pENTR_L1-L4_NN_G1333-DddtoxA-C, 
                     【E1】pENTR_L1-L4_NN_G1333-DddtoxA-N, 
                     【E1】pENTR_L1-L4_NN_G1397-DddtoxA-C, 
                     【E1】pENTR_L1-L4_NN_G1397-DddtoxA-N, 
                     pK7WG2DIPSHSP_ORF125_1.xdna, pK7WG2DIPSHSP_ORF125_2.xdna, 
                     pH7WG2DIPS_ORF79cTAL14.xdna, pH7WG2DIPS_ORF79cTAL8.xdna"
                     /note="/Transferred_Similarity=100.00% -> 99.26%"
                     /note="/X-Transferred_From=pH7WG2DIPS_ORF79cTAL14.xdna;pH7W
                     G2DIPS_ORF79cTAL8.xdna; pK7WG2DIPSHSP_ORF125_1.xdna; 
                     pK7WG2DIPSHSP_ORF125_2.xdna; 
                     【E1】pENTR_L1-L4_NG_G1333-DddtoxA-C; 
                     【E1】pENTR_L1-L4_NG_G1333-DddtoxA-N; 
                     【E1】pENTR_L1-L4_NG_G1397-DddtoxA-C; 
                     【E1】pENTR_L1-L4_NG_G1397-DddtoxA-N; 
                     【E1】pENTR_L1-L4_NI_G1333-DddtoxA-C; 
                     【E1】pENTR_L1-L4_NI_G1333-DddtoxA-N; 
                     【E1】pENTR_L1-L4_NI_G1397-DddtoxA-C; 
                     【E1】pENTR_L1-L4_NI_G1397-DddtoxA-N; 
                     【E1】pENTR_L1-L4_NN_G1333-DddtoxA-C; 
                     【E1】pENTR_L1-L4_NN_G1333-DddtoxA-N; 
                     【E1】pENTR_L1-L4_NN_G1397-DddtoxA-C; 
                     【E1】pENTR_L1-L4_NN_G1397-DddtoxA-N"
                     /note="/X-Transferred_Similarity=100.00% -> 99.26%; 99.75% 
                     ->99.26%"
     CDS             complement(3547..3615)
                     /label=LacZ alpha
     primer_bind     3686..3703
                     /label=M13-fwd
     primer_bind     3713..3732
                     /label=T7
     primer_bind     complement(3777..3796)
                     /label=T3
     primer_bind     complement(3814..3834)
                     /label=M13-rev
     misc_binding    complement(3840..3862)
                     /label=LacO
     promoter        complement(3867..3896)
                     /label=lac
     misc_feature    join(3899..4033,1..480)
                     /label=ORF 4 (frame 2)
                     /label=nonstandard type: ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=2"
                     /note="/created_by=ORF Finder"
     misc_feature    3974..4030
                     /label=NG_lastR_pt
     misc_feature    join(4031..4033,1..120)
                     /label=C-term region_Mok2020
                     /note="/modified_by=Issei.N"
ORIGIN
        1 attgttgccc agttatctcg ccctgatccg gcgttggccg cgttgaccaa cgaccacctc
       61 gtcgccttgg cctgcctcgg cgggcgtcct gcgctggatg cagtgaaaaa gggattgggg
      121 ggttctgcga ttccagttaa gagaggagct acaggagaaa cgaaagtctt tactgggaat
      181 tccaattctc ccaaatcacc gactaaaggc ggatgtagtg gtggtagtac caatctttcc
      241 gacattatcg agaaggaaac aggtaaacaa ctcgtaatcc aagaaagcat actgatgctt
      301 cctgaagagg ttgaagaggt catagggaac aaacctgaaa gcgacatttt ggttcatact
      361 gcctatgatg agtctacaga tgagaacgtg atgttgctaa cctcagatgc acctgaatac
      421 aagccatggg ctttagtgat tcaggattcg aatggagaga acaagatcaa gatgctctga
      481 taactcgagc gatcctctag aacccagctt tcttgtacaa agttggcatt ataagaaagc
      541 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
      601 gccatccagc tgcagggcgg ccgcgatatc ccctatagtg agtcgtatta catggtcata
      661 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
      721 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
      781 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
      841 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
      901 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
      961 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     1021 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     1081 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     1141 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     1201 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     1261 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     1321 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     1381 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     1441 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     1501 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     1561 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     1621 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     1681 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1741 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1801 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1861 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     1921 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1981 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2041 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2101 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     2161 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2221 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2281 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2341 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2401 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2461 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     2521 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     2581 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     2641 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     2701 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     2761 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     2821 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     2881 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     2941 agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac ctgttcgttg
     3001 caacaaattg atgagcaatg cttttttata atgccaactt tgtataataa agttgataag
     3061 gagaccgccg ctgccaagtt cgagagacag cacatggaca gcatcgatat cgccgatcta
     3121 cgcacgctcg gctacagcca gcagcaacag gagaagatca aaccgaaggt tcgttcgaca
     3181 gtggcgcagc accacgaggc actggtcggc cacgggttta cacacgcgca catcgttgcg
     3241 ttaagccaac acccggcagc gttagggacc gtcgctgtca agtatcagga catgatcgca
     3301 gcgttgccag aggcgacaca cgaagcgatc gttggcgtcg gcaaacagtg gtccggcgca
     3361 cgcgctctgg aggccttgct cacggtggcg ggagagttga gaggtccacc gttacagttg
     3421 gacacaggcc aacttctcaa gattgcaaaa cgtggcggcg tgaccgcagt ggaggcagtg
     3481 catgcatggc gcaatgcact gacgggtgcc ccgctcaagg agacgggcgc cgctacaggg
     3541 cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     3601 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     3661 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga
     3721 ctcactatag ggcgaattgg gtaccgggcc ccccctcgag gtcctccagc ttttgttccc
     3781 tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     3841 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     3901 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     3961 accggtcgtc tccacccctg agcaggtagt ggctattgca tccaacggag ggggcagacc
     4021 cgcactggag tca
//
