LOCUS       Exported                4267 bp ds-DNA     circular SYN 15-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...3j-ddcav01
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 17, 2021 from SnapGene Viewer 5.2.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    121..126
                     /label=2aa linker
                     /note="/created_by=Issei.N"
                     /note="/modified_by=Issei.N"
     misc_feature    122..711
                     /label=ATCCTATCAGCCGTTCCCAGCTGGTGAAGTCCGAGCTGGAGGAGAAG...
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=ATCCTATCAGCCGTTCCCAGCTGGTGAAGTCCGAGC
                     TGGAG 
                     GAGAAGAAATCCGAGTTGAGGCACAAGCTGAAGTACGTGCCCCACGAGTACATCGAGC 
                     TGATCGAGATCGCCCGGAACAGCACCCAGGACCGTATCCTGGAGATGAAGGTGATGGA 
                     GTTCTTCATGAAGGTGTACGGCTACAGGGGCAAGCACCTGGGCGGCTCCAGGAAGCCC 
                     GACGGCGCCATCTACACCGTGGGCTCCCCCATCGACTACGGCGTGATCGTGGACACCA 
                     AGGCCTACTCCGGCGGCTACAACCTGCCCATCGGCCAGGCCGACGAAATGCAGAGGTA 
                     CGTGGAGGAGAACCAGACCAGGAACAAGCACATCAACCCCAACGAGTGGTGGAAGGTG 
                     TACCCCTCCAGCGTGACCGAGTTCAAGTTCCTGTTCGTGTCCGGCCACTTCAAGGGCA 
                     ACTACAAGGCCCAGCTGACCAGGCTGAACCACATCACCAACTGCAACGGCGCCGTGCT 
                     GTCCGTGGAGGAGCTCCTGATCGGCGGCGAGATGATCAAGGCCGGCACCCTGACCCTG 
                     GAGGAGGTGAGGAGGAAGTTCAACAACGGCGAGATCAACTTCGCGGCCGAC"
     misc_feature    127..450
                     /label=G1397-DddtoxA-N
                     /note="/created_by=Issei.N"
                     /note="/modified_by=Issei.N"
     misc_feature    451..462
                     /label=4aa linker
                     /note="/created_by=Issei.N"
     misc_feature    463..711
                     /label=UGI
                     /note="Derived using Geneious Prime 2020.1.2 'Annotatefrom 
                     Database' based on nucleotide similarity"
                     /note="/Alternative_Match=79.52%; Adjusted-Interval-0:0/0; 
                     UGI(<a href=""urn:local:.:5k-c662uqm"">2aa linker + 
                     G1333-DddtoxA-C + 4aa linker + 1*UGI     </a>)"
                     /note="/Primary_Match=79.92%; Adjusted-Interval-0:0/0; UGI 
                     (<a href=""urn:local:.:5s-c662uqm"">2aa linker + 
                     G1397-DddtoxA-C + 4aa linker + 1*UGI </a>)"
                     /note="/Transferred_From=2aa linker + G1397-DddtoxA-C + 
                     4aalinker + 1*UGI "
                     /note="/Transferred_Similarity=79.92%"
                     /note="/X-Alternative_Match=79.52%; 
                     Adjusted-Interval-0:0/0; UGI(<a 
                     href=""""urn:local:.:5k-c662uqm"""">2aa linker + 
                     G1333-DddtoxA-C + 4aa linker + 1*UGI     </a>)"
                     /note="/X-Primary_Match=83.13%; Adjusted-Interval-0:0/0; 
                     UGI (<a href=""""urn:local:.:5t-c662uqm"""">2aa linker + 
                     G1333-DddtoxA-N + 4aa linker + 1*UGI     </a>)"
                     /note="/X-Transferred_From=2aa linker + G1333-DddtoxA-N + 
                     4aalinker + 1*UGI     "
                     /note="/X-Transferred_Similarity=83.13%"
                     /note="/created_by=Issei.N"
     misc_recomb     complement(739..834)
                     /label=AttL2
     primer_bind     complement(865..884)
                     /label=T7
     primer_bind     complement(885..905)
                     /label=M13-rev
     CDS             1018..1824
                     /label=KanR
     rep_origin      1952..2580
                     /direction=RIGHT
                     /label=ColE1 origin
     terminator      complement(2892..2919)
                     /label=rrnB T2
     terminator      complement(3051..3094)
                     /label=rrnB T1
     primer_bind     3160..3177
                     /label=M13-fwd
     misc_recomb     3194..3289
                     /label=attL3
     misc_recomb     complement(3269..3289)
                     /label=attB3
     CDS             3292..3336
                     /label=S-tag
     misc_feature    3346..3753
                     /label=N-term region_wako; N-term region_wako compact;...
                     /note="/Transferred_From=【E1】pENTR_L1-L4_HD_G1333-DddtoxA-C
                     , 【E1】pENTR_L1-L4_HD_G1333-DddtoxA-N, 
                     【E1】pENTR_L1-L4_HD_G1397-DddtoxA-C, 
                     【E1】pENTR_L1-L4_HD_G1397-DddtoxA-N, 
                     【E1】pENTR_L1-L4_NG_G1333-DddtoxA-C, 
                     【E1】pENTR_L1-L4_NG_G1333-DddtoxA-N, 
                     【E1】pENTR_L1-L4_NG_G1397-DddtoxA-C, 
                     【E1】pENTR_L1-L4_NG_G1397-DddtoxA-N, 
                     【E1】pENTR_L1-L4_NI_G1333-DddtoxA-C, 
                     【E1】pENTR_L1-L4_NI_G1333-DddtoxA-N, 
                     【E1】pENTR_L1-L4_NI_G1397-DddtoxA-C, 
                     【E1】pENTR_L1-L4_NI_G1397-DddtoxA-N, 
                     【E1】pENTR_L1-L4_NN_G1333-DddtoxA-C, 
                     【E1】pENTR_L1-L4_NN_G1333-DddtoxA-N, 
                     【E1】pENTR_L1-L4_NN_G1397-DddtoxA-C, 
                     【E1】pENTR_L1-L4_NN_G1397-DddtoxA-N, 
                     pK7WG2DIPSHSP_ORF125_1.xdna, pK7WG2DIPSHSP_ORF125_2.xdna, 
                     pH7WG2DIPS_ORF79cTAL14.xdna, pH7WG2DIPS_ORF79cTAL8.xdna"
                     /note="/Transferred_Similarity=100.00% -> 99.26%"
                     /note="/X-Transferred_From=pH7WG2DIPS_ORF79cTAL14.xdna;pH7W
                     G2DIPS_ORF79cTAL8.xdna; pK7WG2DIPSHSP_ORF125_1.xdna; 
                     pK7WG2DIPSHSP_ORF125_2.xdna; 
                     【E1】pENTR_L1-L4_NG_G1333-DddtoxA-C; 
                     【E1】pENTR_L1-L4_NG_G1333-DddtoxA-N; 
                     【E1】pENTR_L1-L4_NG_G1397-DddtoxA-C; 
                     【E1】pENTR_L1-L4_NG_G1397-DddtoxA-N; 
                     【E1】pENTR_L1-L4_NI_G1333-DddtoxA-C; 
                     【E1】pENTR_L1-L4_NI_G1333-DddtoxA-N; 
                     【E1】pENTR_L1-L4_NI_G1397-DddtoxA-C; 
                     【E1】pENTR_L1-L4_NI_G1397-DddtoxA-N; 
                     【E1】pENTR_L1-L4_NN_G1333-DddtoxA-C; 
                     【E1】pENTR_L1-L4_NN_G1333-DddtoxA-N; 
                     【E1】pENTR_L1-L4_NN_G1397-DddtoxA-C; 
                     【E1】pENTR_L1-L4_NN_G1397-DddtoxA-N"
                     /note="/X-Transferred_Similarity=100.00% -> 99.26%; 99.75% 
                     ->99.26%"
     CDS             complement(3781..3849)
                     /label=LacZ alpha
     primer_bind     3920..3937
                     /label=M13-fwd
     primer_bind     3947..3966
                     /label=T7
     primer_bind     complement(4011..4030)
                     /label=T3
     primer_bind     complement(4048..4068)
                     /label=M13-rev
     misc_binding    complement(4074..4096)
                     /label=LacO
     promoter        complement(4101..4130)
                     /label=lac
     misc_feature    join(4133..4267,1..714)
                     /label=ORF 4 (frame 2)
                     /label=nonstandard type: ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=2"
                     /note="/created_by=ORF Finder"
     misc_feature    4208..4264
                     /label=NG_lastR_pt
     misc_feature    join(4265..4267,1..120)
                     /label=C-term region_Mok2020
                     /note="/modified_by=Issei.N"
ORIGIN
        1 attgttgccc agttatctcg ccctgatccg gcgttggccg cgttgaccaa cgaccacctc
       61 gtcgccttgg cctgcctcgg cgggcgtcct gcgctggatg cagtgaaaaa gggattgggg
      121 gggtctggat cgtatgcttt aggaccgtat cagatctcag ctccacaatt gcctgcatat
      181 aacggacaaa ctgttgggac cttttactac gttaacgatg ctggtggatt ggagtccaaa
      241 gtgttctctt ctggtggccc aactccatat cccaattatg cgaatgcagg ccatgttgaa
      301 ggtcaatcag ccctattcat gagagataac ggaataagtg aaggactggt gtttcacaac
      361 aatccagaag gtacttgtgg attttgcgta aacatgactg agacacttct cccagaaaat
      421 gccaagatga cagttgtacc tcctgaaggt tctggtggat cgacaaacct ttcagacatt
      481 atcgagaaag agacaggcaa acagctagtg attcaagagt ccattctcat gcttcccgaa
      541 gaagttgagg aagtcattgg gaataagccg gaaagtgaca tactcgttca tacggcttac
      601 gatgagagca cggatgagaa tgtcatgttg cttaccagtg atgcacctga atacaaacct
      661 tgggctttag tcatccagga cagcaatggt gagaacaaga tcaagatgct gtgataactc
      721 gagcgatcct ctagaaccca gctttcttgt acaaagttgg cattataaga aagcattgct
      781 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
      841 cagctgcagg gcggccgcga tatcccctat agtgagtcgt attacatggt catagctgtt
      901 tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
      961 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
     1021 agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta
     1081 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg
     1141 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
     1201 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
     1261 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga
     1321 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
     1381 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc
     1441 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
     1501 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
     1561 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
     1621 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
     1681 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
     1741 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     1801 catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag
     1861 agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt
     1921 tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     1981 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2041 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2101 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     2161 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2221 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2281 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2341 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2401 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2461 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2521 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2581 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     2641 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     2701 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     2761 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     2821 tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt
     2881 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag
     2941 tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg
     3001 ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
     3061 aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc
     3121 gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt
     3181 aagctcgggc cccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
     3241 attgatgagc aatgcttttt tataatgcca actttgtata ataaagttga taaggagacc
     3301 gccgctgcca agttcgagag acagcacatg gacagcatcg atatcgccga tctacgcacg
     3361 ctcggctaca gccagcagca acaggagaag atcaaaccga aggttcgttc gacagtggcg
     3421 cagcaccacg aggcactggt cggccacggg tttacacacg cgcacatcgt tgcgttaagc
     3481 caacacccgg cagcgttagg gaccgtcgct gtcaagtatc aggacatgat cgcagcgttg
     3541 ccagaggcga cacacgaagc gatcgttggc gtcggcaaac agtggtccgg cgcacgcgct
     3601 ctggaggcct tgctcacggt ggcgggagag ttgagaggtc caccgttaca gttggacaca
     3661 ggccaacttc tcaagattgc aaaacgtggc ggcgtgaccg cagtggaggc agtgcatgca
     3721 tggcgcaatg cactgacggg tgccccgctc aaggagacgg gcgccgctac agggcgcgtc
     3781 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     3841 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     3901 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact
     3961 atagggcgaa ttgggtaccg ggccccccct cgaggtcctc cagcttttgt tccctttagt
     4021 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     4081 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     4141 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccaccggt
     4201 cgtctccacc cctgagcagg tagtggctat tgcatccaac ggagggggca gacccgcact
     4261 ggagtca
//
