LOCUS       pCAG-WIPI2dC93E-cs-TEV- 6275 bp ds-DNA     circular     18-MAY-2021
DEFINITION  .
KEYWORDS    "marker:AmpR"                                                      
FEATURES             Location/Qualifiers
     CDS             complement(273..1064)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(1065..1133)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        complement(1134..1238)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     enhancer        1268..1647
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        1649..1926
                     /label="chicken β-actin promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     intron          1927..2943
                     /label="chimeric intron"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
     CDS             3023..4297
                     /label="Translation 3023-4297"
     misc_feature    3023..4297
                     /label="WIPI2d"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3809..3910
                     /label="Tom loop delete"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             4298..4364
                     /label="Translation 4298-4364"
     CDS             4304..4324
                     /label="TEV site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     regulatory      4329..4338
                     /label="Kozak sequence"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             4340..4363
                     /label="Strep-Tag II"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             4400..4423
                     /label="Strep-Tag II"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     polyA_signal    4606..4661
                     /label="β-globin poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(5022..5038)
                     /label="M13 rev"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     protein_bind    5046..5062
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        complement(5070..5076)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(5077..5094)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(5095..5100)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    5115..5136
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        5194..5410
                     /label="SV40 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      5241..5396
                     /label="SV40 ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     polyA_signal    5416..5550
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(join(5789..6275,1..102))
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
       61 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      121 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      181 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      241 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
      301 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
      361 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
      421 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
      481 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
      541 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
      601 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
      661 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
      721 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
      781 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
      841 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
      901 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
      961 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1021 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1081 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     1141 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     1201 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     1261 cctggtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     1321 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1381 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     1441 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     1501 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     1561 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     1621 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
     1681 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
     1741 gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag
     1801 gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga
     1861 aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg
     1921 cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgcgcc gcctcgcgcc
     1981 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt
     2041 ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc
     2101 gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg
     2161 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg
     2221 agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg
     2281 ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg
     2341 gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc
     2401 ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg
     2461 gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc
     2521 ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc
     2581 gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag
     2641 agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc
     2701 cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc
     2761 ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc
     2821 tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg
     2881 cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta
     2941 cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattcctc
     3001 gatcgacggt ATCGATgcca ccATGAACCT GGCGAGCCAG AGCGGGGAGG CCGGCGCCGG
     3061 CCAGCTGCTC TTCGCCAACT TCAACCAGGA CAACACGTCC CTAGCTGTTG GTAGTAAGTC
     3121 CGGTTATAAA TTTTTCTCCC TTTCTTCTGT GGATAAGCTG GAACAGATCT ATGAATGCAC
     3181 CGATACGGAA GATGTGTGCA TTGTAGAGAG ATTGTTCTCC AGCAGCCTAG TGGCCATCGT
     3241 GAGCCTTAAA GCACCAAGGA AGCTAAAGGT TTGCCACTTT AAGAAGGGAA CTGAGATCGA
     3301 AAACTACAGC TACTCCAACA CGATTCTGGC TGTGAAGCTC AACAGGCAGA GGCTGATAGT
     3361 ATGCCTGGAG GAGTCCCTGT ACATCCACAA CATTCGGGAC ATGAAGGTGC TGCATACGAT
     3421 CAGGGAGACG CCTCCAAACC CTGCAGGCCT GTGTGCGCTG TCAATCAACA ACGACAACTG
     3481 CTACTTGGCG TACCCAGGGA GCGCGACCAT CGGAGAGGTG CAGGTCTTCG ATACCATTAA
     3541 TTTGAGAGCT GCAAACATGA TTCCGGCTCA CGACAGTCCT TTAGCGGCAC TGGCCTTTGA
     3601 CGCAAGTGGA ACTAAACTTG CCACGGCTTC GGAGAAGGGG ACCGTGATTA GGGTATTTTC
     3661 CATTCCAGAA GGACAAAAAC TCTTTGAGTT TCGGAGAGGA GTAAAGAGGT GCGTGAGCAT
     3721 CTGCTCCCTG GCCTTCAGCA TGGACGGCAT GTTCCTCTCC GCCTCCAGCA ACACTGAGAC
     3781 CGTGCACATC TTCAAACTCG AGACTGTGAA AGAAAAACCC CCAGAGGAGC CCACCACCTG
     3841 GACCGGGTAC TTCGGGAAAG TGCTCATGGC CTCCACCAGC TACCTGCCTT CCCAAGTGAC
     3901 AGAAATGTTC AACCAGGGCA GAGCCTTCGC CACGGTCCGC CTGCCATTCT GCGGCCACAA
     3961 AAACATCTGC TCGCTAGCCA CAATTCAGAA GATCCCGCGG TTGTTGGTGG GTGCCGCCGA
     4021 CGGGTACCTG TACATGTACA ACCTGGACCC CCAGGAGGGC GGCGAGTGTG CCCTGATGAA
     4081 GCAGCACCGG CTGGACGGCA GTCTGGAAAC GACCAATGAG ATCTTGGACT CTGCCTCTCA
     4141 CGACTGCCCC TTAGTCACTC AGACATACGG CGCAGCTGCA GGAAAAGCCT ACACAGACGA
     4201 CCTGGGTGCT GTGGGTGGCG CCTGCCTGGA GGACGAGGCC AGCGCCCTGC GCCTGGATGA
     4261 GGACAGCGAG CACCCGCCCA TGATTCTTCG GACTGACGGA TCGGAAAACC TGTATTTTCA
     4321 GGGTGGTACC ATGGCGGCCT GGTCACACCC GCAGTTCGAG AAAGGTGGAG GTGCCCGAGG
     4381 TGGATCGGGA GGTGGATCGT GGAGCCACCC GCAGTTCGAA AAATGACCCG GGtgatatct
     4441 taattaatta actcgaggaa ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg
     4501 ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa
     4561 attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta
     4621 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     4681 gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc
     4741 atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc
     4801 cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta
     4861 tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac
     4921 tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat
     4981 ggagatccct cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     5041 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     5101 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     5161 ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag
     5221 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     5281 cccatggctg actaattttt tttatttatg cagaggccgC CTCTACAAAT GTGGTATGGa
     5341 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5401 cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     5461 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     5521 ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc caacgcgcgg
     5581 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     5641 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     5701 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     5761 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     5821 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     5881 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     5941 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
     6001 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6061 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6121 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6181 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     6241 gtatctgcgc tctgctgaag ccagttacct tcgga
//