LOCUS       pCAG-mcherry-WIPI2dC93E 6996 bp ds-DNA     circular     18-MAY-2021
DEFINITION  .
KEYWORDS    "marker:AmpR"                                                      
FEATURES             Location/Qualifiers
     CDS             145..849
                     /label="mCherry"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     CDS             850..885
                     /label="Translation 850-885"
     misc_feature    886..2160
                     /label="WIPI2d "
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             886..2160
                     /label="Translation 886-2160"
     CDS             2167..2187
                     /label="TEV site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     regulatory      2192..2201
                     /label="Kozak sequence"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             2203..2226
                     /label="Strep-Tag II"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             2263..2286
                     /label="Strep-Tag II"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     polyA_signal    2469..2524
                     /label="β-globin poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(2885..2901)
                     /label="M13 rev"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     protein_bind    2909..2925
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        complement(2933..2939)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(2940..2957)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(2958..2963)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    2978..2999
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        3057..3253
                     /label="SV40 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      3104..3239
                     /label="SV40 ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     polyA_signal    3259..3393
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(3632..4220)
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(4391..5182)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(5183..5251)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        complement(5252..5356)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     enhancer        5386..5765
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        5767..6044
                     /label="chicken β-actin promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     intron          join(6045..6996,1..65)
                     /label="chimeric intron"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
ORIGIN
        1 ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc
       61 tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattcc
      121 tcgatcgacg gtATCGATgc caccGTGAGC AAGGGCGAGG AGGATAACAT GGCCATCATC
      181 AAGGAGTTCA TGCGCTTCAA GGTGCACATG GAGGGCTCCG TGAACGGCCA CGAGTTCGAG
      241 ATCGAGGGCG AGGGCGAGGG CCGCCCCTAC GAGGGCACCC AGACCGCCAA GCTGAAGGTG
      301 ACCAAGGGTG GCCCCCTGCC CTTCGCCTGG GACATCCTGT CCCCTCAGTT CATGTACGGC
      361 TCCAAGGCCT ACGTGAAGCA CCCCGCCGAC ATCCCCGACT ACTTGAAGCT GTCCTTCCCC
      421 GAGGGCTTCA AGTGGGAGCG CGTGATGAAC TTCGAGGACG GCGGCGTGGT GACCGTGACC
      481 CAGGACTCCT CCCTGCAGGA CGGCGAGTTC ATCTACAAGG TGAAGCTGCG CGGCACCAAC
      541 TTCCCCTCCG ACGGCCCCGT AATGCAGAAG AAGACCATGG GCTGGGAGGC CTCCTCCGAG
      601 CGGATGTACC CCGAGGACGG CGCCCTGAAG GGCGAGATCA AGCAGAGGCT GAAGCTGAAG
      661 GACGGCGGCC ACTACGACGC TGAGGTCAAG ACCACCTACA AGGCCAAGAA GCCCGTGCAG
      721 CTGCCCGGCG CCTACAACGT CAACATCAAG TTGGACATCA CCTCCCACAA CGAGGACTAC
      781 ACCATCGTGG AACAGTACGA ACGCGCCGAG GGCCGCCACT CCACCGGCGG CATGGACGAG
      841 CTGTACAAGT CCGGACTCAG ATCTCGAGCT CAAGCTTCGA ATTCGATGAA CCTGGCGAGC
      901 CAGAGCGGGG AGGCCGGCGC CGGCCAGCTG CTCTTCGCCA ACTTCAACCA GGACAACACG
      961 TCCCTAGCTG TTGGTAGTAA GTCCGGTTAT AAATTTTTCT CCCTTTCTTC TGTGGATAAG
     1021 CTGGAACAGA TCTATGAATG CACCGATACG GAAGATGTGT GCATTGTAGA GAGATTGTTC
     1081 TCCAGCAGCC TAGTGGCCAT CGTGAGCCTT AAAGCACCAA GGAAGCTAAA GGTTTGCCAC
     1141 TTTAAGAAGG GAACTGAGAT CGAAAACTAC AGCTACTCCA ACACGATTCT GGCTGTGAAG
     1201 CTCAACAGGC AGAGGCTGAT AGTATGCCTG GAGGAGTCCC TGTACATCCA CAACATTCGG
     1261 GACATGAAGG TGCTGCATAC GATCAGGGAG ACGCCTCCAA ACCCTGCAGG CCTGTGTGCG
     1321 CTGTCAATCA ACAACGACAA CTGCTACTTG GCGTACCCAG GGAGCGCGAC CATCGGAGAG
     1381 GTGCAGGTCT TCGATACCAT TAATTTGAGA GCTGCAAACA TGATTCCGGC TCACGACAGT
     1441 CCTTTAGCGG CACTGGCCTT TGACGCAAGT GGAACTAAAC TTGCCACGGC TTCGGAGAAG
     1501 GGGACCGTGA TTAGGGTATT TTCCATTCCA GAAGGACAAA AACTCTTTGA GTTTCGGAGA
     1561 GGAGTAAAGA GGTGCGTGAG CATCTGCTCC CTGGCCTTCA GCATGGACGG CATGTTCCTC
     1621 TCCGCCTCCA GCAACACTGA GACCGTGCAC ATCTTCAAAC TCGAGACTGT GAAAGAAAAA
     1681 CCCCCAGAGG AGCCCACCAC CTGGACCGGG TACTTCGGGA AAGTGCTCAT GGCCTCCACC
     1741 AGCTACCTGC CTTCCCAAGT GACAGAAATG TTCAACCAGG GCAGAGCCTT CGCCACGGTC
     1801 CGCCTGCCAT TCTGCGGCCA CAAAAACATC TGCTCGCTAG CCACAATTCA GAAGATCCCG
     1861 CGGTTGTTGG TGGGTGCCGC CGACGGGTAC CTGTACATGT ACAACCTGGA CCCCCAGGAG
     1921 GGCGGCGAGT GTGCCCTGAT GAAGCAGCAC CGGCTGGACG GCAGTCTGGA AACGACCAAT
     1981 GAGATCTTGG ACTCTGCCTC TCACGACTGC CCCTTAGTCA CTCAGACATA CGGCGCAGCT
     2041 GCAGGAAAAG CCTACACAGA CGACCTGGGT GCTGTGGGTG GCGCCTGCCT GGAGGACGAG
     2101 GCCAGCGCCC TGCGCCTGGA TGAGGACAGC GAGCACCCGC CCATGATTCT TCGGACTGAC
     2161 ggatcgGAAA ACCTGTATTT TCAGGGTGGT ACCatggcgg cctggtcaca cccgcagttc
     2221 gagaaaggtg gaggtgcccg aggtggatcg ggaggtggat cgtggagcca cccgcagttc
     2281 gaaaaaTGAC CCGGGtgata tcttaattaa ttaactcgag gaattcactc ctcaggtgca
     2341 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
     2401 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
     2461 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     2521 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
     2581 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
     2641 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
     2701 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
     2761 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
     2821 catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat
     2881 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     2941 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     3001 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatccgca
     3061 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     3121 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     3181 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     3241 aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3301 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     3361 aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg
     3421 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     3481 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     3541 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     3601 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     3661 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     3721 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     3781 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
     3841 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     3901 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3961 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     4021 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     4081 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4141 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     4201 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     4261 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     4321 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     4381 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     4441 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     4501 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     4561 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     4621 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     4681 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     4741 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     4801 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     4861 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     4921 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     4981 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     5041 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     5101 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     5161 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     5221 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     5281 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     5341 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tggtcgacat tgattattga
     5401 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     5461 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     5521 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     5581 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     5641 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     5701 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     5761 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
     5821 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
     5881 ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga
     5941 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
     6001 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct
     6061 gccttcgccc cgtgccccgc tccgcgccgc ctcgcgccgc ccgccccggc tctgactgac
     6121 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg
     6181 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg
     6241 ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg
     6301 gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg
     6361 gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg
     6421 cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
     6481 agggggtgtg ggcgcgtcgg tcgggctgca acccccccct gcacccccct ccccgagttg
     6541 ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg
     6601 tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg
     6661 gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg
     6721 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt
     6781 cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg
     6841 gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc
     6901 cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc
     6961 ttcggggggg acggggcagg gcggggttcg gcttct
//