LOCUS       pAAV-hSyn1-DIO-ExRai-AK 5286 bp ds-DNA     circular     08-MAY-2024
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name:
            paav-hsyn1-dio-exrai-akar2-cw3sl.gb
FEATURES             Location/Qualifiers
     LTR             1..141
                     /label="ITR 5'"
     misc_feature    1..785
                     /label="1 to 4779 of pAAV-hSyn-DIO-mCherry ver3 [Split]"
     ITR element     12..141
                     /label="Flop"
     ITR element     complement(38..46)
                     /label="ITR-C"
     Should avoid    complement(46..50)
                     /label="rep-binding seq"
     ITR element     50..58
                     /label="ITR-C"
     ITR element     complement(60..68)
                     /label="ITR-B"
     Should avoid    68..72
                     /label="rep-binding seq"
     ITR element     72..80
                     /label="ITR-B"
     ITR element     81..121
                     /label="ITR-A"
     Should avoid    complement(116..121)
                     /label="trs"
     Should avoid    118..123
                     /label="trs (AAV2)"
     ITR element     complement(121..141)
                     /label="ITR-D"
     regulatory      150..617
                     /label="hSyn1 promoter"
     misc_feature    618..651
                     /label="Lox2272"
     misc_feature    682..715
                     /label="LoxP"
     CDS             complement(786..2003)
                     /label="exRai AKAR2"
                     /translation="MLRRATLVDGGTGGSELSFCNVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNLLHKFSQEQIGENIVCRVICTTGQIPIRDLSADISQVLKEKRSIKKVWTFGRNPACDYHLGNISRLSNKHFQILLGEDGNLLLNDISTNGTWLNGQKVEKNSNQLLSQGDEITVGVGVESDILSLVIFINDKFKQCLEQNKVDR*"
     CDS             complement(786..2003)
                     /label="Translation 2003-786"
                     /translation="MLRRATLVDGGTGGSELSFCNVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNLLHKFSQEQIGENIVCRVICTTGQIPIRDLSADISQVLKEKRSIKKVWTFGRNPACDYHLGNISRLSNKHFQILLGEDGNLLLNDISTNGTWLNGQKVEKNSNQLLSQGDEITVGVGVESDILSLVIFINDKFKQCLEQNKVDR*"
     Should avoid    797..801
                     /label="rep-binding seq"
     misc_feature    complement(2015..2048)
                     /label="Lox2272 (inv)"
     misc_feature    complement(2079..2112)
                     /label="LoxP (inv)"
     Should avoid    2210..2214
                     /label="rep-binding seq"
     regulatory      2361..2540
                     /label="SL polyA signal"
     ITR element     2549..2569
                     /label="ITR-D"
     ITR element     complement(2549..2678)
                     /label="Flop"
     LTR             2550..2690
                     /label="ITR 3'"
     Should avoid    complement(2567..2572)
                     /label="trs (AAV2)"
     Should avoid    2569..2574
                     /label="trs"
     ITR element     complement(2569..2609)
                     /label="ITR-A"
     ITR element     complement(2610..2618)
                     /label="ITR-B"
     Should avoid    complement(2618..2622)
                     /label="rep-binding seq"
     ITR element     2622..2630
                     /label="ITR-B"
     ITR element     complement(2632..2640)
                     /label="ITR-C"
     Should avoid    2640..2644
                     /label="rep-binding seq"
     ITR element     2644..2652
                     /label="ITR-C"
     ITR element     2653..2693
                     /label="ITR-A variant 1"
     Should avoid    complement(2749..2753)
                     /label="rep-binding seq"
     rep_origin      2764..3219
                     /label="M13 origin"
     rep_origin      2781..3087
                     /label="F1 ori"
     Should avoid    3031..3035
                     /label="rep-binding seq"
     gene            3606..4466
                     /label="bla"
     Should avoid    complement(3715..3720)
                     /label="trs (AAV2)"
     CDS             3804..4463
                     /label="AmpR"
                     /translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     Should avoid    complement(4001..4006)
                     /label="trs"
     Should avoid    4003..4008
                     /label="trs (AAV2)"
     rep_origin      4611..5284
                     /label="pUC origin"
     Should avoid    complement(4625..4629)
                     /label="rep-binding seq"
     Should avoid    4728..4733
                     /label="trs (AAV2)"
     ITR element     complement(5283..37)
                     /label="ITR-A variant 2"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC
       61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA
      121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCT GCAGAGGGCC CTGCGTATGA GTGCAAGTGG
      181 GTTTTAGGAC CAGGATGAGG CGGGGTGGGG GTGCCTACCT GACGACCGAC CCCGACCCAC
      241 TGGACAAGCA CCCAACCCCC ATTCCCCAAA TTGCGCATCC CCTATCAGAG AGGGGGAGGG
      301 GAAACAGGAT GCGGCGAGGC GCGTGCGCAC TGCCAGCTTC AGCACCGCGG ACAGTGCCTT
      361 CGCCCCCGCC TGGCGGCGCG CGCCACCGCC GCCTCAGCAC TGAAGGCGCG CTGACGTCAC
      421 TCGCCGGTCC CCCGCAAACT CCCCTTCCCG GCCACCTTGG TCGCGTCCGC GCCGCCGCCG
      481 GCCCAGCCGG ACCGCACCAC GCGAGGCGCG AGATAGGGGG GCACGGGCGC GACCATCTGC
      541 GCTGCGGCGC CGGCGACTCA GCGCTGCCTC AGTCTGCGGT GGGCAGCGGA GGAGTCGTGT
      601 CGTGCCTGAG AGCGCAGATA ACTTCGTATA GGATACTTTA TACGAAGTTA TGCAGAATGG
      661 TAGCTGGATT GTAGCTGCTA TATAACTTCG TATAGCATAC ATTATACGAA GTTATCTAAA
      721 TTTTAACACT CTCGATGCCA TATACGTTGT AACCTTCCTT ATAAGTTGCA AAATTCTGGG
      781 AATTCCTAGC GATCAACTTT GTTCTGCTCG AGGCACTGCT TAAATTTGTC GTTTATGAAA
      841 ATGACCAGAG ATAAAATATC TGATTCCACG CCTACACCAA CGGTTATTTC ATCACCTTGA
      901 GACAGTAACT GATTGCTGTT CTTCTCGACT TTTTGCCCAT TTAACCAGGT CCCATTAGTG
      961 GAAATGTCAT TCAATAAAAG GTTACCGTCT TCTCCTAGTA GTATTTGGAA ATGCTTATTT
     1021 GACAGTCTTG AAATGTTTCC TAAATGATAG TCACAGGCTG GGTTTCTACC AAATGTCCAA
     1081 ACTTTCTTTA TGGATCGTTT TTCCTTAAGC ACTTGTGAAA TATCAGCTGA CAAATCTCGG
     1141 ATGGGAATTT GACCCGTGGT ACAAATGACC CTGCACACAA TGTTTTCGCC GATCTGTTCT
     1201 TGAGAAAACT TATGCAGCAA GTTGTACTCC AGCTTGTGCC CCAGGATGTT GCCGTCCTCC
     1261 TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC GAACTTCACC
     1321 TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC CTGGATGTAG
     1381 CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG GTAGCGGCTG
     1441 AAGCACTGCA CGCCGTAGGT CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC GGGCAGCTTG
     1501 CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCGTAGG TGGCATCGCC CTCACCCTCG
     1561 CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG GATGGGCACC
     1621 ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGCTCC CTCCGGTACC GCCCTTGTAC
     1681 AGCTCGTCCA TGCCGAGAGT GATCCCGGCG GCGGTCACGA ACTCCAGCAG GACCATGTGA
     1741 TCGCGCTTCT CGTTGGGGTC TTTCGAAAGT TTGGACTGCA CGCTCAGGTA GTGGTTGTCG
     1801 GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT GGTAGTGGTA GGCGAGCTGC
     1861 ACGCCGCCGT CCTCGATGTT GTGGCGGATC TTGAAGTTCG CCTTGATGCC GTTCTTCTGC
     1921 TTGTCGGCCT TGATATAGAC GTTGCAGAAG CTGAGCTCGC TGCCGCCGGT GCCGCCGTCC
     1981 ACCAGGGTGG CGCGACGCAG CATGGTGGCT CGAGATAACT TCGTATAAAG TATCCTATAC
     2041 GAAGTTATTT GCCTTAACCC AGAAATTATC ACTGTTATAT AACTTCGTAT AATGTATGCT
     2101 ATACGAAGTT ATGATAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT
     2161 CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT
     2221 GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTAGTTCTT
     2281 GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG
     2341 GGCACTGACA ATTCCGTGGT GTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA
     2401 ACCATCTAGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG
     2461 CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA
     2521 GATGTGGGAG GTTTTTTAAA GCGGCCGCAG GAACCCCTAG TGATGGAGTT GGCCACTCCC
     2581 TCTCTGCGCG CTCGCTCGCT CACTGAGGCC GGGCGACCAA AGGTCGCCCG ACGCCCGGGC
     2641 TTTGCCCGGG CGGCCTCAGT GAGCGAGCGA GCGCGCAGCT GCCTGCAGGG GCGCCTGATG
     2701 CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATACGTCA AAGCAACCAT
     2761 AGTACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA
     2821 CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG
     2881 CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT
     2941 TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTT GGGTGATGGT TCACGTAGTG
     3001 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA
     3061 GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGGCTAT TCTTTTGATT
     3121 TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT
     3181 TTAACGCGAA TTTTAACAAA ATATTAACGT TTACAATTTT ATGGTGCACT CTCAGTACAA
     3241 TCTGCTCTGA TGCCGCATAG TTAAGCCAGC CCCGACACCC GCCAACACCC GCTGACGCGC
     3301 CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA
     3361 GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGACGA AAGGGCCTCG
     3421 TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG
     3481 GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA
     3541 ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA
     3601 AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC
     3661 TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG
     3721 GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC
     3781 GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT
     3841 TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG
     3901 ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG
     3961 AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA
     4021 CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC
     4081 GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA
     4141 CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC
     4201 TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC
     4261 TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG
     4321 GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA
     4381 TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG
     4441 GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA
     4501 TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC
     4561 TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA
     4621 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
     4681 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC
     4741 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT
     4801 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC
     4861 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC
     4921 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA
     4981 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG
     5041 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG
     5101 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT
     5161 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT
     5221 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC
     5281 ACATGT
//