LOCUS       Exported                2287 bp ds-DNA     circular SYN 19-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   John Heap
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 19, 2021 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..72
                     /label=SS 2
     primer_bind     complement(55..71)
                     /label=OligoGT262
     promoter        144..248
                     /gene="bla"
                     /label=AmpR promoter
     CDS             249..1109
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(298..317)
                     /label=OligoGT235
     primer_bind     complement(952..987)
                     /label=Primer SS 4
     primer_bind     952..987
                     /label=Primer SS 3
     rep_origin      1280..1868
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1768..1786
                     /label=OligoGT234
     primer_bind     1999..2014
                     /label=OligoGT261
     primer_bind     complement(2004..2065)
                     /label=SS 1
     terminator      2026..2120
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(2100..2135)
                     /label=OligoGT336
     primer_bind     complement(2100..2135)
                     /label=OligoGT332
     primer_bind     complement(2100..2135)
                     /label=OligoGT330
     primer_bind     complement(2100..2135)
                     /label=OligoGT334
     primer_bind     complement(2112..2128)
                     /label=Primer SS 5
     promoter        2140..2184
                     /label=SPLc19
     RBS             2185..2213
                     /label=RBSc123
     primer_bind     2229..2245
                     /label=OligoGT335
     primer_bind     2229..2245
                     /label=OligoGT329
     primer_bind     2229..2245
                     /label=OligoGT331
     primer_bind     2229..2245
                     /label=OligoGT333
     terminator      join(2246..2287,1..45)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc cgaaagggcc
       61 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
      121 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
      181 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      241 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      301 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      361 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      421 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      481 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      541 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      601 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      661 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      721 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      781 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      841 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      901 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      961 gtgggtcccg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     1021 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     1081 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1141 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1201 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1261 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1321 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1381 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1441 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1501 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1561 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1621 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1681 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1741 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1801 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1861 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1921 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1981 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg ccttggactc ctgttgatag
     2041 atccagtaat gacctcagaa ctccatctgg atttgttcag aacgctcggt tgccgccggg
     2101 cgttttttat tggtgagaat ccagtgtggc tcttcgcagg cggctcgtat tgacaattta
     2161 actaaatatc agtataattt agtttgtgta ggtcccacca aaggaggtct gtaatgtgaa
     2221 gagcgtaaga cctctagggc ggcggatttg tcctactcag gagagcgttc accgacaaac
     2281 aacagat
//
