LOCUS       pCMV-MCS-eDHFR(69K6)-EGFP 5300 bp ds-DNA     circular     07-NOV-2021
DEFINITION  .
KEYWORDS    "Creator:Sachio Suzuki"                                            
FEATURES             Location/Qualifiers
     promoter        10..562
                     /label="CMV_immearly_promoter"
     regulatory      65..352
                     /label="CAG_enhancer"
     primer          446..467
                     /label="TY-seq-FRB(5)"
                     /note="sequence: GTCAATGGGAGTTTGTTTTGGC"
     primer          514..533
                     /label="TY6(5)seq_MNL"
                     /note="sequence: ATTGACGCAAATGGGCGGTA"
     primer          519..539
                     /label="CMV_fwd_primer"
     promoter        520..589
                     /label="CMV_promoter"
     primer          complement(568..588)
                     /label="MY(3)-CMV-promoter-BamHI"
                     /note="sequence: GATAGCGGATCCATCTGACGGTTCACTAAACCA"
     primer          586..603
                     /label="EI-3(5)_NheI-EGFP"
                     /note="sequence: GATCCGCTAGCGCTACCG"
     CDS             592..1965
                     /label="Translation 592-1965"
     misc_feature    610..654
                     /label="MCS"
     misc_feature    655..720
                     /label="Linker 22aa"
     misc_feature    721..1215
                     /label="DHFR(69K6)"
     primer          1246..1263
                     /label="ANP(5)_mCherry-term(ERK-KTR)"
                     /note="sequence: ATGGTGAGCAAGGGCGAG"
     gene            1249..1962
                     /label="EGFP"
     primer          complement(1291..1312)
                     /label="EGFP_N_primer"
     primer          complement(1314..1331)
                     /label="KK_Seq(3)-EGFP"
                     /note="sequence: CTGAACTTGTGGCCGTTT"
     feature         1429..1458
                     /label="Y66 (EGFP)"
     primer          1843..1861
                     /label="TY-seq-(5)-EGFP-C-term"
                     /note="sequence: cactacctgagcacccagt"
     primer          1899..1920
                     /label="EGFP_C_primer"
     primer          2179..2198
                     /label="EBV_rev_primer"
     origin          complement(2348..2654)
                     /label="f1_origin"
     promoter        2733..2761
                     /label="AmpR_promoter"
     primer          complement(2827..2847)
                     /label="pBABE_3_primer"
     regulatory      complement(2833..3048)
                     /label="SV40_enhancer"
     promoter        2845..3113
                     /label="SV40_promoter"
     origin          3012..3089
                     /label="SV40_origin"
     primer          3074..3093
                     /label="SV40pro_F_primer"
     gene            3199..3987
                     /label="NeoR/KanR"
     terminator      4165..4434
                     /label="TK_PA_terminator"
     origin          4582..5201
                     /label="pBR322_origin"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caAGCTTGCG ATCGCTGCAG CCCGGGCCGC GGCTGGCTCT
      661 GGTGCTGGTA GTGGCGCTGG TTCCGGTGCT GGCTCTGGCG CGtcgacgGT ACCCGCCACC
      721 ATGATCAGTC TGATTGCGGC GTTAGCGGTA GATCGCGTTA TCGGCATGGA AAACGCCATG
      781 CCGTGGAACC TGCCTGCCGA TCTCGCCTGG TTTAAACGCA ACACCTTAAA TAAACCCGTG
      841 ATTATGGGCC GCCATACCTG GGAATCAATC GGTCGTCCGT TGCCAGGACG CAAAAATATT
      901 ATCCTCAGCA GTCAACCGGG TACGGACAAA AAAAAGAAAA AGAAAGATCG CGTAACGTGG
      961 GTGAAGTCGG TGGATGAAGC CATCGCGGCG TGTGGTGACG TACCAGAAAT CATGGTGATT
     1021 GGCGGCGGTC GCGTTTATGA ACAGTTCTTG CCAAAAGCGC AAAAACTGTA TCTGACGCAT
     1081 ATCGACGCAG AAGTGGAAGG CGACACCCAT TTCCCGGATT ACGAGCCGGA TGACTGGGAA
     1141 TCGGTATTCA GCGAATTCCA CGATGCTGAT GCGCAGAACT CTCACAGCTA TTGCTTTGAG
     1201 ATTCTGGAGC GGCGGAGCGG CTCCGGggat ccaccggtcg ccaccatggt gagcaagggc
     1261 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     1321 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     1381 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     1441 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     1501 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     1561 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1621 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1681 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     1741 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1801 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     1861 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1921 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgactct
     1981 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     2041 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     2101 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     2161 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt
     2221 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     2281 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     2341 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     2401 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     2461 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     2521 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     2581 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     2641 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     2701 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2761 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc
     2821 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     2881 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     2941 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     3001 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     3061 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3121 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga
     3181 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     3241 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     3301 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     3361 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     3421 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     3481 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     3541 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     3601 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     3661 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     3721 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     3781 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     3841 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     3901 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     3961 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     4021 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     4081 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     4141 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac
     4201 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt
     4261 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
     4321 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
     4381 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag
     4441 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     4501 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     4561 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     4621 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     4681 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     4741 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     4801 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     4861 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     4921 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     4981 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5041 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     5101 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     5161 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     5221 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     5281 accgtattac cgccatgcat
//