LOCUS       Exported                5727 bp DNA     circular SYN 28-MAY-2021
DEFINITION  pCDFDuet.
ACCESSION   urn.local...2d-dfavci0
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 4, 2021 from SnapGene 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(1..28)
                     /label=lacO reg
                     /note="Geneious type: regulatory sequence"
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=lacO reg"
                     /note="/Transferred_Similarity=82.14%"
     misc_feature    2..29
                     /label=lacO reg
                     /note="Geneious type: regulatory sequence"
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=lacO reg"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    2..29
                     /label=lacO
     promoter        complement(6..29)
                     /label=amp prom
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=amp prom"
                     /note="/Transferred_Similarity=58.62%"
     gene            47..63
                     /label=T7_transl_en_RBS
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=T7_transl_en_RBS"
                     /note="/Transferred_Similarity=94.12%"
     misc_feature    57..103
                     /label=T710RBSL reg
                     /note="Geneious type: regulatory sequence"
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=T710RBSL reg"
                     /note="/Transferred_Similarity=57.45%"
     misc_feature    180..202
                     /label=323
                     /note="/created_by=User"
     promoter        200..273
                     /label=trc prom
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=trc prom"
                     /note="/Transferred_Similarity=47.30%"
     promoter        214..232
                     /label=T7_promoter
     misc_feature    complement(231..258)
                     /label=lacO reg
                     /note="Geneious type: regulatory sequence"
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=lacO reg"
                     /note="/Transferred_Similarity=82.14%"
     misc_feature    232..259
                     /label=lacO reg
                     /note="Geneious type: regulatory sequence"
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=lacO reg"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    232..259
                     /label=lacO
                     /label=lacO(1)
     promoter        complement(236..259)
                     /label=amp prom
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=amp prom"
                     /note="/Transferred_Similarity=58.62%"
     misc_feature    complement(246..291)
                     /label=367
                     /note="/created_by=User"
     misc_feature    complement(256..291)
                     /label=365
                     /note="/created_by=User"
     misc_feature    complement(262..303)
                     /label=415
                     /note="/created_by=User"
     misc_feature    complement(262..291)
                     /label=317
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    274..316
                     /label=414
                     /note="/created_by=User"
     gene            275..291
                     /label=T7_transl_en_RBS
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=T7_transl_en_RBS"
                     /note="/Transferred_Similarity=88.24%"
     misc_feature    292..2237
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    292..1383
                     /label=pylC-opt 0.69
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    1458..2237
                     /label=pylD-opt 0.67
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    complement(1462..1486)
                     /label=383
                     /note="/created_by=User"
     misc_feature    complement(2198..2267)
                     /label=355
                     /note="/created_by=User"
     misc_feature    complement(2200..2267)
                     /label=353
                     /note="/created_by=User"
     misc_feature    2208..2273
                     /label=342
                     /note="/created_by=User"
     misc_feature    2208..2271
                     /label=340
                     /note="/created_by=User"
     misc_feature    complement(2211..2267)
                     /label=315
                     /note="/created_by=User"
     misc_feature    2238..2273
                     /label=366
                     /note="/created_by=User"
     misc_feature    2238..2271
                     /label=364
                     /note="/created_by=User"
     misc_feature    2238..2263
                     /label=316
                     /note="/created_by=User"
     misc_feature    complement(2391..2412)
                     /label=324
                     /note="/created_by=User"
     misc_feature    complement(2394..2412)
                     /label=T7_Terminal_primer
     terminator      2413..2456
                     /label=T7 term
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=T7 term"
                     /note="/Transferred_Similarity=97.73%"
     terminator      2413..2448
                     /label=T7_terminator
     misc_feature    complement(2626..3636)
                     /label=spect marker
                     /label=nonstandard type: marker
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=spect marker"
                     /note="/Transferred_Similarity=83.28%"
     gene            complement(2626..3417)
                     /gene="spect (variant)"
                     /label=spect (variant)
     promoter        complement(3459..3487)
                     /label=AmpR_promoter
                     /note="/translation=MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVD
                     GGLKP 
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP 
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ 
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA 
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK*"
     misc_feature    3460..3487
                     /label=lacO reg
                     /note="Geneious type: regulatory sequence"
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=lacO reg"
                     /note="/Transferred_Similarity=53.57%"
     misc_feature    complement(3667..4296)
                     /label=pBR322 origin
                     /note="Geneious type: origin of replication"
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=pBR322 origin"
                     /note="/Transferred_Similarity=72.33%"
     misc_feature    complement(4360..4386)
                     /label=HA tag
                     /label=nonstandard type: tag
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=HA tag"
                     /note="/Transferred_Similarity=55.56%"
     terminator      4458..4488
                     /label=trpA term
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=trpA term"
                     /note="/Transferred_Similarity=64.52%"
     misc_feature    complement(4505..5596)
                     /label=lacI reg
                     /note="Geneious type: regulatory sequence"
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=lacI reg"
                     /note="/Transferred_Similarity=99.91%"
     misc_feature    complement(4505..5596)
                     /label=lacI
                     /note="/translation=MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVD
                     GGLKP 
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP 
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ 
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA 
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK*"
     promoter        complement(4660..4688)
                     /label=ARA prom
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=ARA prom"
                     /note="/Transferred_Similarity=51.72%"
     promoter        join(5697..5727,1..43)
                     /label=trc prom
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=trc prom"
                     /note="/Transferred_Similarity=48.65%"
     promoter        join(5711..5727,1..2)
                     /label=T7 prom
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=T7 prom"
                     /note="/Transferred_Similarity=100.00%"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
       61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag
      121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag
      181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt
      241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatgaaaacc
      301 atctgcctga ttggtggtaa attgcagggt tttgaggccg cgtatctgtc taaaaaagcc
      361 gatatgaaag tagtggttat cgacaagaac ccccaagctc ttatccgcaa ttacgcagac
      421 gagtttcatt gttttgatgt tatcaaggaa ccagagaaac ttcttgaaat cagcaaaaat
      481 gtagacgcta tcttgcccgt caacgaaaac ttagaatgta ttaaattctt aagtagtgta
      541 aaagaggaat tttcttgccc agtactgttc gattttgagg cttaccgcat ctcgcgcgat
      601 aagaagaaat cgaaagagta tttccgttcc atcggcattc cgacaccgca agacaaacct
      661 ggtcgtcctc catacttcgt taagccaccg tgcgaaagct cgtcggttgg tgctcgcatt
      721 atctacgatg aagaagaact gggagaattg gagcccggca tgctggtaga agagtatgtg
      781 gaaggtgagg ttatttcact tgaagtaatc ggggacggta cccactttgc agttgttaaa
      841 gagacactta ttcacatcga ccgcacctat gactgccata tggtaactcc cctgccatct
      901 gatttgtctt tccgtgagat cagctattct ttagcggcca acctgccttt gaaggggatc
      961 atggacgttg aagcaatttc gggcccgcaa ggtttgaagg tcattgaaat tgattcacgt
     1021 ttcccctccc agacccccac agccgtgtat tattcgtcgg gagtgaattt aattgagctg
     1081 ttgtttcgtg cctttggaga aggggtggag gaagttaaaa cccttccaga ggaccgctac
     1141 tgtatctacg agcacctgat gcttgcagaa aatggggccc tgatcccagt aggtgagcag
     1201 gttctgtcga tggggaatga ctatgggaaa tactatgaag aaccaggaat tgaaatcttt
     1261 ttatgtcgcg gagaggaccc cgtgtttacg cttgtctttt ggggaaagga ccgtgaggaa
     1321 gccgaaaaca aaaagcgcac tggtttgctg attttgaagg accgcttcgg ggcagccgtt
     1381 taaaatggca aaaatcggat aagaataaaa atttgcccaa agtaaaattt gtttaaaaca
     1441 gaaaaaggag ataaaaaatg gctttgttga ctcccgagga cctggagaac attaaccgtc
     1501 aacttcacga agccgattct acggtacgtc gtgtgactgg gttagatatc aagggagttt
     1561 gtaaagattt ctatgggact actccatgct gtgagaaggt cggtatcgtc ccggtaacta
     1621 gtggcaacgg catcattggc aacttttctg aaagtcttcg tgcgatcact gagtactttg
     1681 gatttgactc attcgtgacg accatgccag acgttagtgg ctactatgag gcggtccgta
     1741 atggagcaca aattattctt atggcagatg ataatacgtt tcttgcacac aatttaaaga
     1801 acggcaagat cgctaataat caaccgtgta ccggaattat ctacgcggag atcgcctcgc
     1861 gctatatgaa agccgattcc aaagatgtgc ttgccgtcgg gcttggaaaa gtggggtttc
     1921 caggcgctgc acatttggtt aataaggggt ttcgtgtgta tgggtacgac gcggataagg
     1981 cgttgttaga aaagactgta tcagaccttg ggatcacacc tttcgagcct gagaatcccc
     2041 gtcgcttctc aatcattttt gaggcgaccc cgtgtgctga tacagtacca gagtctgtca
     2101 tcagcgacaa ttgtgtcatc tctacgcccg gtatcccttg cgcgatcagc cgtgaacttc
     2161 aagaaaaata cggggtggag ttgattatgg aacctctggg tattggaact gcttctatgc
     2221 tgtatagcat tttgtaaata tacatatggc agatctcaat tggatatcgg ccggccacgc
     2281 gatcgctgac gtcggtaccc tcgagtctgg taaagaaacc gctgctgcga aatttgaacg
     2341 ccagcacatg gactcgtcta ctagcgcagc ttaattaacc taggctgctg ccaccgctga
     2401 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
     2461 acctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
     2521 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcatc
     2581 gtggccggat cttgcggccc ctcggcttga acgaattgtt agacattatt tgccgactac
     2641 cttggtgatc tcgcctttca cgtagtggac aaattcttcc aactgatctg cgcgcgaggc
     2701 caagcgatct tcttcttgtc caagataagc ctgtctagct tcaagtatga cgggctgata
     2761 ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc
     2821 ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc
     2881 ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg
     2941 ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc
     3001 tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc
     3061 tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg
     3121 ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcgct
     3181 ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc
     3241 atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgtggct tcaggccgcc
     3301 atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat
     3361 gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt ccctcatact
     3421 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3481 atttgaatgt atttagaaaa ataaacaaat agctagctca ctcggtcgct acgctccggg
     3541 cgtgagactg cggcgggcgc tgcggacaca tacaaagtta cccacagatt ccgtggataa
     3601 gcaggggact aacatgtgag gcaaaacagc agggccgcgc cggtggcgtt tttccatagg
     3661 ctccgccctc ctgccagagt tcacataaac agacgctttt ccggtgcatc tgtgggagcc
     3721 gtgaggctca accatgaatc tgacagtacg ggcgaaaccc gacaggactt aaagatcccc
     3781 accgtttccg gcgggtcgct ccctcttgcg ctctcctgtt ccgaccctgc cgtttaccgg
     3841 atacctgttc cgcctttctc ccttacggga agtgtggcgc tttctcatag ctcacacact
     3901 ggtatctcgg ctcggtgtag gtcgttcgct ccaagctggg ctgtaagcaa gaactccccg
     3961 ttcagcccga ctgctgcgcc ttatccggta actgttcact tgagtccaac ccggaaaagc
     4021 acggtaaaac gccactggca gcagccattg gtaactggga gttcgcagag gatttgttta
     4081 gctaaacacg cggttgctct tgaagtgtgc gccaaagtcc ggctacactg gaaggacaga
     4141 tttggttgct gtgctctgcg aaagccagtt accacggtta agcagttccc caactgactt
     4201 aaccttcgat caaaccacct ccccaggtgg ttttttcgtt tacagggcaa aagattacgc
     4261 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tactgaaccg ctctagattt
     4321 cagtgcaatt tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt
     4381 aattctcatg ttagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct
     4441 caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt
     4501 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     4561 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca
     4621 gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc
     4681 ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga
     4741 tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatgtcc gcaccaacgc
     4801 gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca
     4861 gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca
     4921 tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt
     4981 tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg
     5041 cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat
     5101 gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa
     5161 cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga
     5221 tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc
     5281 cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa
     5341 tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca
     5401 gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg
     5461 ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca
     5521 cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg
     5581 gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa
     5641 aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc
     5701 attaggaaat taatacgact cactata
//
