LOCUS       Exported                8178 bp DNA     circular SYN 28-MAY-2021
DEFINITION  [ColE1] PproK_PylT (PSP_pIII(Ser/Pro11*)_lux).
ACCESSION   urn.local...j-3hmsdrb
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 4, 2021 from SnapGene 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8178
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1304..1363
                     /note="/dnas_title=BH10T (-62 Medium Linker Omega Cterm)
                     Adjusted with Position 10 T"
     source          1364..1565
                     /label=source(1)
                     /note="/dnas_title=Reverse pJC173b - AP pSC101 pT7 SD8 RBS
                     g3dp-luxAB ggggs fusion"
     source          5207..6466
                     /note="/dnas_title=CE-SP-T7 P314T"
     source          7171..7174
                     /label=source(2)
                     /note="/dnas_title=BH10T (-62 Medium Linker Omega Cterm)
                     Adjusted with Position 10 T"
     rep_origin      1..588
                     /label=ColE1 origin of replication
     CDS             complement(1016..1207)
                     /label=Rop
                     /note="Rop"
     misc_feature    1305..7174
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     terminator      1319..1363
                     /note="rrnB1 transcriptional terminator"
     misc_feature    1364..1370
                     /label=Nt-BbvCI
                     /note="Nt-BbvCI"
                     /note="nicking endonuclease site that generates overhang"
     misc_feature    1378..1384
                     /label=SanDI
                     /note="SanDI"
                     /note="Keep unique in all constructs, can be used to
                     exchange f1 origin or selection components"
     promoter        1385..1552
                     /label=Phage Shock Promoter
                     /note="/created_by=User"
     misc_feature    1553..1560
                     /label=SbfI
                     /note="SbfI"
     misc_feature    1560..1565
                     /label=BsgI
                     /note="BsgI"
     RBS             1567..1581
                     /label=SD8 RBS
                     /note="SD8 RBS"
     CDS             1582..2856
                     /label=gIII
                     /note="/created_by=User"
     variation       1666..1668
                     /label=Ser/Pro11TAG
                     /label=nonstandard type: mutation
                     /note="/created_by=User"
                     /note="/modified_by=User"
     CDS             2856..4979
                     /label=xluxAB
                     /note="xluxAB"
     misc_feature    3413..3438
                     /note="/created_by=User"" /label /label=DB26(1)
                     /ApEinfo_label=DB26 /ApEinfo_fwdcolor=pink 
                     /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data 
                     {{0 1 2 0 0 -1} {} 0} width 5 offset 0"
     primer_bind     3561..3585
                     /label=JC497
                     /note="JC497"
     sig_peptide     3939..3998
                     /note="GGGGS4 flexible linker"
     misc_feature    4801..4826
                     /label=224
                     /note="/created_by=User"
     terminator      4994..5027
                     /label=BBa B1002 terminator
                     /note="/created_by=User"
     terminator      complement(5029..5067)
                     /label=BBa B1006 terminator
                     /note="/created_by=User"
     misc_feature    complement(5055..5107)
                     /label=321
                     /note="/created_by=User"
     misc_feature    complement(5056..5107)
                     /label=322
                     /note="/created_by=User"
     misc_feature    complement(5074..6673)
                     /label=AP_delPT_R
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    5078..6655
                     /label=Move lpp-chPS into AP pDB038a
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    5078..5101
                     /label=pTECH-ins-Fseq1
                     /note="/created_by=User"
     misc_feature    5078..5100
                     /label=318
                     /note="/created_by=User"
     misc_feature    5081..5101
                     /label=pTECH-ins-Fseq
                     /note="/created_by=User"
     misc_feature    complement(5100..5134)
                     /label=407
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    5104..5166
                     /label=410
                     /note="/created_by=User"
     misc_feature    5135..5187
                     /label=408
                     /note="/created_by=User"
     promoter        5135..5164
                     /label=lpp prom
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=lpp prom"
                     /note="/Transferred_Similarity=90.00%"
     misc_feature    5135..5140
                     /label=-35 e
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    complement(5157..5206)
                     /label=409
                     /note="/created_by=User"
     misc_feature    5159..5164
                     /label=-10 G
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    complement(5178..5236)
                     /label=411
                     /note="/created_by=User"
     RBS             5192..5206
                     /label=SD8 RBS
                     /note="SD8 RBS"
     CDS             5207..6466
                     /label=PylS-CH
                     /note="/created_by=User"
     misc_feature    5207..6466
                     /label=chPS
                     /note="/created_by=User"
     misc_feature    5207..5231
                     /label=406
                     /note="/created_by=User"
     misc_feature    5663..6463
                     /label=2ZIN PDB
                     /label=nonstandard type: region
                     /note="/created_by=User"
     misc_feature    5663..5665
                     /label=Residue 188 in 2ZIN
                     /label=nonstandard type: region
                     /note="/created_by=User"
     misc_feature    6137..6139
                     /label=near BocK
                     /label=nonstandard type: region
                     /note="/created_by=User"
     misc_feature    6143..6145
                     /label=near BocK
                     /label=near BocK(1)
                     /label=nonstandard type: region
                     /note="/created_by=User"
     misc_feature    6350..6352
                     /label=near BocK
                     /label=near BocK(2)
                     /label=nonstandard type: region
                     /note="/created_by=User"
     misc_feature    6356..6358
                     /label=near BocK
                     /label=near BocK(3)
                     /label=nonstandard type: region
                     /note="/created_by=User"
     misc_feature    complement(6467..6479)
                     /label=pT-pC_R
                     /note="/created_by=User"
     misc_feature    complement(6548..6570)
                     /label=pTECH-ins-Rseq
                     /note="/created_by=User"
     misc_feature    complement(6556..6575)
                     /label=pTECH-ins-Rseq2
                     /note="/created_by=User"
     misc_feature    6626..6675
                     /label=320
                     /note="/created_by=User"
     misc_feature    complement(6634..6655)
                     /label=319
                     /note="/created_by=User"
     promoter        6666..6816
                     /label=proK
                     /note="/created_by=User"
     misc_feature    complement(6733..6755)
                     /label=325
                     /note="/created_by=User"
                     /note="/modified_by=User"
     tRNA            6846..6917
                     /label=PylT
                     /note="/created_by=User"
     misc_feature    6923..6943
                     /label=del_PT_F
                     /note="/created_by=User"
     terminator      6936..6970
                     /label=rrnC terminator
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    complement(7151..7174)
                     /label=225
                     /note="/created_by=User"
     misc_feature    7175
                     /label=pDB209
                     /note="Geneious type: Concatenated sequence"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     terminator      complement(7199..7240)
                     /note="tetA/orfL bidirectional terminator"
                     /note="termination of bla transcript is slightly weaker
                     than opposite direction"
     CDS             complement(7254..8045)
                     /label=aadA (specR)
                     /note="/dnas_title=aadA"
                     /note="/vntifkey=4"
     promoter        8046..8130
                     /note="aadA promoter"
     misc_feature    8162..8163
                     /label=AGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGC
                     /label=AGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCG
                     /note="Geneious type: Editing History Deletion"
                     /note="/Original_Bases=AGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAA
                     TCGGC 
                     CAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACC 
                     AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCA 
                     AGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAATGG 
                     CGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCA 
                     CCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGT 
                     TGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTG 
                     AAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTG 
                     CGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATG 
                     GGCCCGCTAACAGTGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAG 
                     TCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACA 
                     TCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGT 
                     CATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCAC 
                     CGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCA 
                     CCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGG 
                     CCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGC 
                     CACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTT 
                     TTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACAC 
                     CGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTG 
                     ACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCAT"
ORIGIN
        1 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
       61 gcggtggttt gtttgccgga tcaagagcta ccacctcttt ttccgaaggt aactggcttc
      121 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
      181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
      361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
      601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
      721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
      781 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1261 aacgagagag gatgctcacg atacgggtta ctgatgatga acaaagagga catccggtca
     1321 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttgctgagg agacttaggg
     1381 accctgatga aattcgccac ttgttagtgt aattcgctaa ctcatcctgg catgttgctg
     1441 ttgattcttc aatcagatct ttataaatca aaaagataaa aaattggcac gcaaattgta
     1501 ttaacagttc agcaggacaa tcctgaacgc agaaatcaag aggacaacat tacctgcagg
     1561 tgcagtaagg aggaaaaaaa aatgaaaaaa ttattattcg caattccttt agttgttcct
     1621 ttctattctc actccgctga aactgttgaa agttgtttag caaaatagca tacagaaaat
     1681 tcatttacta acgtctggaa agacgacaaa actttagatc gttacgctaa ctatgagggc
     1741 tgtctgtgga atgctacagg cgttgtagtt tgtactggtg acgaaactca gtgttacggt
     1801 acatgggttc ctattgggct tgctatccct gaaaatgagg gtggtggctc tgagggtggc
     1861 ggttctgagg gtggcggttc tgagggtggc ggtactaaac ctcctgagta cggtgataca
     1921 cctattccgg gctatactta tatcaaccct ctcgacggca cttatccgcc tggtactgag
     1981 caaaaccccg ctaatcctaa tccttctctt gaggagtctc agcctcttaa tactttcatg
     2041 tttcagaata ataggttccg aaataggcag ggggcattaa ctgtttatac gggcactgtt
     2101 actcaaggca ctgaccccgt taaaacttat taccagtaca ctcctgtatc atcaaaagcc
     2161 atgtatgacg cttactggaa cggtaaattc agagactgcg ctttccattc tggctttaat
     2221 gaggatccat tcgtttgtga atatcaaggc caatcgtctg acctgcctca acctcctgtc
     2281 aatgctggcg gcggctctgg tggtggttct ggtggcggct ctgagggtgg tggctctgag
     2341 ggtggcggtt ctgagggtgg cggctctgag ggaggcggtt ccggtggtgg ctctggttcc
     2401 ggtgattttg attatgaaaa gatggcaaac gctaataagg gggctatgac cgaaaatgcc
     2461 gatgaaaacg cgctacagtc tgacgctaaa ggcaaacttg attctgtcgc tactgattac
     2521 ggtgctgcta tcgatggttt cattggtgac gtttccggcc ttgctaatgg taatggtgct
     2581 actggtgatt ttgctggctc taattcccaa atggctcaag tcggtgacgg tgataattca
     2641 cctttaatga ataatttccg tcaatattta ccttccctcc ctcaatcggt tgaatgtcgc
     2701 ccttttgtct ttggcgctgg taaaccttac gagttcagta tcgactgcga taagatcaac
     2761 ctgttccgcg gtgtctttgc gtttctttta tatgttgcca cctttatgta tgtattttct
     2821 acgtttgcta acatactgcg taataaggag tcttaatgaa atttggaaac tttttgctta
     2881 cataccaacc tccccaattt tcccaaacag aggtaatgaa acgtttggtt aaattaggtc
     2941 gcatctctga ggagtgtggt tttgataccg tatggttact ggagcatcat ttcacggagt
     3001 ttggtttgct tggtaaccct tatgtcgctg ctgcatattt acttggcgcg actaaaaaat
     3061 tgaatgtagg aactgccgct attgttcttc ccacagccca tccagtacgc caacttgaag
     3121 atgtgaattt attggatcaa atgtcaaaag gacgatttcg gtttggtatt tgccgagggc
     3181 tttacaacaa ggactttcgc gtattcggca cagatatgaa taacagtcgc gccttagcgg
     3241 aatgctggta cgggctgata aagaatggca tgacagaggg atatatggaa gctgataatg
     3301 aacatatcaa gttccataag gtaaaagtaa accccgcggc gtatagcaga ggtggcgcac
     3361 cggtttatgt ggtggctgaa tcagcttcga cgactgagtg ggctgctcaa tttggcctac
     3421 cgatgatatt aagttggatt ataaatacta acgaaaagaa agcacaactt gagctttata
     3481 atgaagtggc tcaagaatat gggcacgata ttcataatat cgaccattgc ttatcatata
     3541 taacatctgt agatcatgac tcaattaaag cgaaagagat ttgccggaaa tttctggggc
     3601 attggtatga ttcttatgtg aatgctacga ctatttttga tgattcagac caaacaagag
     3661 gttatgattt caataaaggg cagtggcgtg actttgtatt aaaaggacat aaagatacta
     3721 atcgccgtat tgattacagt tacgaaatca atcccgtggg aacgccgcag gaatgtattg
     3781 acataattca aaaagacatt gatgctacag gaatatcaaa tatttgttgt ggatttgaag
     3841 ctaatggaac agtagacgaa attattgctt ccatgaagct cttccagtct gatgtcatgc
     3901 catttcttaa agaaaaacaa cgttcgctat tatattatgg cggtggcggt agcggcggtg
     3961 gcggtagcgg cggtggcggt agcggcggtg gcggtagcaa atttggattg ttcttcctta
     4021 acttcatcaa ttcaacaact gttcaagaac agagtatagt tcgcatgcag gaaataacgg
     4081 agtatgttga taagttgaat tttgaacaga ttttagtgta tgaaaatcat ttttcagata
     4141 atggtgttgt cggcgctcct ctgactgttt ctggttttct gctcggttta acagagaaaa
     4201 ttaaaattgg ttcattaaat cacatcatta caactcatca tcctgtccgc atagcggagg
     4261 aagcttgctt attggatcag ttaagtgaag ggagatttat tttagggttt agtgattgcg
     4321 aaaaaaaaga tgaaatgcat ttttttaatc gcccggttga atatcaacag caactatttg
     4381 aagagtgtta tgaaatcatt aacgatgctt taacaacagg ctattgtaat ccagataacg
     4441 atttttatag cttccctaaa atatctgtaa atccccatgc ttatacgcca ggcggacctc
     4501 ggaaatatgt aacagcaacc agtcatcata ttgttgagtg ggcggccaaa aaaggtattc
     4561 ctctcatctt taagtgggat gattctaatg atgttagata tgaatatgct gaaagatata
     4621 aagccgttgc ggataaatat gacgttgacc tatcagagat agaccatcag ttaatgatat
     4681 tagttaacta taacgaagat agtaataaag ctaaacaaga gacgcgtgca tttattagtg
     4741 attatgttct tgaaatgcac cctaatgaaa atttcgaaaa taaacttgaa gaaataattg
     4801 cagaaaacgc tgtcggaaat tatacggagt gtataactgc ggctaagttg gcaattgaaa
     4861 agtgtggtgc gaaaagtgta ttgctgtcct ttgaaccaat gaatgatttg atgagccaaa
     4921 aaaatgtaat caatattgtt gatgataata ttaagaagta ccacacggaa tatacctaaa
     4981 cggagccaat gtacgcaaaa aaccccgctt cagcggggtt ttttcgccaa aaaaaacccc
     5041 gccctgtcag gggcggggtt tttttttagg gccctacgtt cgatgcttct ttgagcgaac
     5101 gatcaaaaat aagtgccttc ccatcaaaaa aatatagaca acataaaaaa ctttgtgtta
     5161 tagttgtaac gctacatgga gattaactca aaaggaggaa aaaaaaatgg ataagaagcc
     5221 gctggatgtt ctgatctctg cgaccggtct gtggatgtcc cgtaccggca cgctgcacaa
     5281 gatcaagcac tatgaggttt ctcgttctaa aatctacatc gaaatggcgt gtggtgacca
     5341 tctggttgtg aacaactctc gttcttgtcg taccgcacgt gcattccgtc atcataaata
     5401 ccgtaaaacc tgcaaacgtt gtcgtgtttc tgacgaagat atcaacaact tcctgacccg
     5461 ttctaccgaa ggcaaaacct ctgttaaagt taaagttgtt tctgcgccga aagtgaaaaa
     5521 agcgatgccg aaatctgttt ctcgtgcgcc gaaaccgctg gaaaatccgg tttctgcgaa
     5581 agcgtctacc gacacctctc gttctgttcc gtctccggcg aaatctaccc cgaactctcc
     5641 ggttccgacc tctgcaagtg cccccgcact tacgaagagc cagactgaca ggcttgaagt
     5701 cctgttaaac ccaaaagatg agatttccct gaattccggc aagcctttca gggagcttga
     5761 gtccgaattg ctctctcgca gaaaaaaaga cctgcagcag atctacgcgg aagaaaggga
     5821 gaattatctg gggaaactcg agcgtgaaat taccaggttc tttgtggaca ggggttttct
     5881 ggaaataaaa tccccgatcc tgatccctct tgagtatatc gaaaggatgg gcattgataa
     5941 tgataccgaa ctttcaaaac agatcttcag ggttgacaag aacttctgcc tgagacccat
     6001 gcttgctcca aacctttaca actacctgcg caagcttgac agggccctgc ctgatccaat
     6061 aaaaattttt gaaataggcc catgctacag aaaagagtcc gacggcaaag aacacctcga
     6121 agagtttacc atgctgaact tctgccagat gggatcggga tgcacacggg aaaatcttga
     6181 aagcataatt acggacttcc tgaaccacct gggaattgat ttcaagatcg taggcgattc
     6241 ctgcatggtc tatggggata cccttgatgt aatgcacgga gacctggaac tttcctctgc
     6301 agtagtcgga cccataccgc ttgaccggga atggggtatt gataaaccct ggataggggc
     6361 aggtttcggg ctcgaacgcc ttctaaaggt taaacacgac tttaaaaata tcaagagagc
     6421 tgcaaggtcc gagtcttact ataacgggat ttctaccaac ctgtaaccta ggctgctgcc
     6481 accgctgaga tctatggccc gcgcccacag gaaggagcta ccggcagcgg tgcggactgt
     6541 tgtaactcat aataagaaat gaggccgctc atggcgttct gttgcccgtc tcgctggtga
     6601 aaagaaaaac caccctggct aactaagcgg cctgctgact ttctcgccga tcaaatgact
     6661 gtccgtgtgc ttctcaaatg cctgaggcca gtttgctcag gctctccccg tggaggtaat
     6721 aattgacgat atgatcagtg cacggctaac taagcggcct gctgactttc tcgccgatca
     6781 aaaggcattt tgctattaag ggattgacga gggcgtatct gcgcagtaag atgcgccccg
     6841 cattcggaaa cctgatcatg tagatcgaat ggactctaaa tccgttcagc cgggttagat
     6901 tcccggggtt tccgccagga tccgtcgacc tgcagatcct tagcgaaagc taaggatttt
     6961 ttttaagctt ctgccattca tccgcttatt atcacttatt caggcgtagc accaggcgtt
     7021 taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc atcgcagtac
     7081 tgttgtaatt cattaagcat tctgccgacg ttcatcggat cccgggcccg tcgactgcag
     7141 aggcctgcat gcaagcttgg cgtaatcatg atggacttaa ttaacggcac tcctcagcaa
     7201 atataatgac cctcttgata acccaagagg gcatttttta atgcccatgg cgtttatttg
     7261 ccgactacct tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg
     7321 cgcgaggcca agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg
     7381 ggctgatact gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg
     7441 attttgccgg ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca
     7501 tcgccagccc agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat
     7561 agatcctgtt caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg
     7621 ctatgttctc ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg
     7681 aagatacctg caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct
     7741 ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga
     7801 atctcgctct ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc
     7861 gttgtttcat caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc
     7921 aggccgccat ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg
     7981 cgctcgatga cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc
     8041 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     8101 ggatacatat ttgaatgtat ttagaaaaat attaattgcg ttgcgctcac tgcccgcttt
     8161 ccaggccaaa taggccgt
//
