LOCUS       Exported                8180 bp DNA     circular SYN 28-MAY-2021
DEFINITION  [ColE1] PproK_PylT (PSP_pIII(Ser/Pro11*)_lux).
ACCESSION   urn.local...j-3hmsdrb
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 4, 2021 from SnapGene 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8180
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1304..1363
                     /note="/dnas_title=BH10T (-62 Medium Linker Omega Cterm)
                     Adjusted with Position 10 T"
     source          1364..1565
                     /label=source(1)
                     /note="/dnas_title=Reverse pJC173b - AP pSC101 pT7 SD8 RBS
                     g3dp-luxAB ggggs fusion"
     source          7173..7176
                     /label=source(2)
                     /note="/dnas_title=BH10T (-62 Medium Linker Omega Cterm)
                     Adjusted with Position 10 T"
     rep_origin      1..588
                     /label=ColE1 origin of replication
     CDS             complement(1016..1207)
                     /label=Rop
                     /note="Rop"
     misc_feature    1305..7176
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     terminator      1319..1363
                     /note="rrnB1 transcriptional terminator"
     misc_feature    1364..1370
                     /label=Nt-BbvCI
                     /note="Nt-BbvCI"
                     /note="nicking endonuclease site that generates overhang"
     misc_feature    1378..1384
                     /label=SanDI
                     /note="SanDI"
                     /note="Keep unique in all constructs, can be used to
                     exchange f1 origin or selection components"
     promoter        1385..1552
                     /label=Phage Shock Promoter
                     /note="/created_by=User"
     misc_feature    1553..1560
                     /label=SbfI
                     /note="SbfI"
     misc_feature    1560..1565
                     /label=BsgI
                     /note="BsgI"
     RBS             1567..1581
                     /label=SD8 RBS
                     /note="SD8 RBS"
     CDS             1582..2856
                     /label=gIII
                     /note="/created_by=User"
                     /note="/modified_by=User"
     variation       1666..1668
                     /label=Ser/Pro11TAG
                     /label=nonstandard type: mutation
                     /note="/created_by=User"
                     /note="/modified_by=User"
     CDS             2856..4979
                     /label=xluxAB
                     /note="xluxAB"
     misc_feature    3413..3438
                     /note="/created_by=User"" /label /label=DB26(1)
                     /ApEinfo_label=DB26 /ApEinfo_fwdcolor=pink 
                     /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data 
                     {{0 1 2 0 0 -1} {} 0} width 5 offset 0"
     primer_bind     3561..3585
                     /label=JC497
                     /note="JC497"
     sig_peptide     3939..3998
                     /note="GGGGS4 flexible linker"
     misc_feature    4801..4826
                     /label=224
                     /note="/created_by=User"
     terminator      4994..5027
                     /label=BBa B1002 terminator
                     /note="/created_by=User"
     terminator      complement(5029..5067)
                     /label=BBa B1006 terminator
                     /note="/created_by=User"
     misc_feature    complement(5055..5107)
                     /label=321
                     /note="/created_by=User"
     misc_feature    complement(5056..5107)
                     /label=322
                     /note="/created_by=User"
     misc_feature    complement(5074..6675)
                     /label=AP_delPT_R
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    5078..6657
                     /label=Move lpp-chPS into AP pDB038a
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    5078..5101
                     /label=pTECH-ins-Fseq1
                     /note="/created_by=User"
     misc_feature    5078..5100
                     /label=318
                     /note="/created_by=User"
     misc_feature    5081..5101
                     /label=pTECH-ins-Fseq
                     /note="/created_by=User"
     promoter        5135..5164
                     /label=lpp prom
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=lpp prom"
                     /note="/Transferred_Similarity=90.00%"
     primer_bind     complement(5178..5238)
                     /label=562
     gene            complement(5187..5208)
                     /label=AraI1I2
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=AraI1I2"
                     /note="/Transferred_Similarity=46.15%"
     misc_feature    5187..5208
                     /label=pTECH_IG_F1
                     /note="/created_by=User"
     misc_feature    5193..5208
                     /label=pTECH_IG_F
                     /note="/created_by=User"
     misc_feature    complement(5209..6468)
                     /label=AP_delPT_R
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    5209..6468
                     /label=Move lpp-chPS into AP pDB038a
                     /note="/created_by=User"
                     /note="/modified_by=User"
     primer_bind     5212..5242
                     /label=563
     primer_bind     complement(6435..6465)
                     /label=564
     primer_bind     6436..6487
                     /label=561
     misc_feature    complement(6469..6481)
                     /label=pT-pC_R
                     /note="/created_by=User"
     misc_feature    complement(6550..6572)
                     /label=pTECH-ins-Rseq
                     /note="/created_by=User"
     misc_feature    complement(6558..6577)
                     /label=pTECH-ins-Rseq2
                     /note="/created_by=User"
     misc_feature    6628..6677
                     /label=320
                     /note="/created_by=User"
     misc_feature    complement(6636..6657)
                     /label=319
                     /note="/created_by=User"
     promoter        6668..6818
                     /label=proK
                     /note="/created_by=User"
     misc_feature    complement(6735..6757)
                     /label=325
                     /note="/created_by=User"
                     /note="/modified_by=User"
     tRNA            6848..6919
                     /label=PylT
                     /note="/created_by=User"
     misc_feature    6925..6945
                     /label=del_PT_F
                     /note="/created_by=User"
     terminator      6938..6972
                     /label=rrnC terminator
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    complement(7153..7176)
                     /label=225
                     /note="/created_by=User"
     misc_feature    7177
                     /label=pDB209
                     /note="Geneious type: Concatenated sequence"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     terminator      complement(7201..7242)
                     /note="tetA/orfL bidirectional terminator"
                     /note="termination of bla transcript is slightly weaker
                     than opposite direction"
     CDS             complement(7256..8047)
                     /label=aadA (specR)
                     /note="/dnas_title=aadA"
                     /note="/vntifkey=4"
     promoter        8048..8132
                     /note="aadA promoter"
     misc_feature    8164..8165
                     /label=AGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGC
                     /label=AGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCG
                     /note="Geneious type: Editing History Deletion"
                     /note="/Original_Bases=AGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAA
                     TCGGC 
                     CAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACC 
                     AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCA 
                     AGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAATGG 
                     CGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCA 
                     CCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGT 
                     TGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTG 
                     AAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTG 
                     CGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATG 
                     GGCCCGCTAACAGTGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAG 
                     TCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACA 
                     TCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGT 
                     CATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCAC 
                     CGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCA 
                     CCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGG 
                     CCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGC 
                     CACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTT 
                     TTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACAC 
                     CGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTG 
                     ACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCAT"
ORIGIN
        1 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
       61 gcggtggttt gtttgccgga tcaagagcta ccacctcttt ttccgaaggt aactggcttc
      121 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
      181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
      361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
      601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
      721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
      781 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1261 aacgagagag gatgctcacg atacgggtta ctgatgatga acaaagagga catccggtca
     1321 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttgctgagg agacttaggg
     1381 accctgatga aattcgccac ttgttagtgt aattcgctaa ctcatcctgg catgttgctg
     1441 ttgattcttc aatcagatct ttataaatca aaaagataaa aaattggcac gcaaattgta
     1501 ttaacagttc agcaggacaa tcctgaacgc agaaatcaag aggacaacat tacctgcagg
     1561 tgcagtaagg aggaaaaaaa aatgaaaaaa ttattattcg caattccttt agttgttcct
     1621 ttctattctc actccgctga aactgttgaa agttgtttag caaaatagca tacagaaaat
     1681 tcatttacta acgtctggaa agacgacaaa actttagatc gttacgctaa ctatgagggc
     1741 tgtctgtgga atgctacagg cgttgtagtt tgtactggtg acgaaactca gtgttacggt
     1801 acatgggttc ctattgggct tgctatccct gaaaatgagg gtggtggctc tgagggtggc
     1861 ggttctgagg gtggcggttc tgagggtggc ggtactaaac ctcctgagta cggtgataca
     1921 cctattccgg gctatactta tatcaaccct ctcgacggca cttatccgcc tggtactgag
     1981 caaaaccccg ctaatcctaa tccttctctt gaggagtctc agcctcttaa tactttcatg
     2041 tttcagaata ataggttccg aaataggcag ggggcattaa ctgtttatac gggcactgtt
     2101 actcaaggca ctgaccccgt taaaacttat taccagtaca ctcctgtatc atcaaaagcc
     2161 atgtatgacg cttactggaa cggtaaattc agagactgcg ctttccattc tggctttaat
     2221 gaggatccat tcgtttgtga atatcaaggc caatcgtctg acctgcctca acctcctgtc
     2281 aatgctggcg gcggctctgg tggtggttct ggtggcggct ctgagggtgg tggctctgag
     2341 ggtggcggtt ctgagggtgg cggctctgag ggaggcggtt ccggtggtgg ctctggttcc
     2401 ggtgattttg attatgaaaa gatggcaaac gctaataagg gggctatgac cgaaaatgcc
     2461 gatgaaaacg cgctacagtc tgacgctaaa ggcaaacttg attctgtcgc tactgattac
     2521 ggtgctgcta tcgatggttt cattggtgac gtttccggcc ttgctaatgg taatggtgct
     2581 actggtgatt ttgctggctc taattcccaa atggctcaag tcggtgacgg tgataattca
     2641 cctttaatga ataatttccg tcaatattta ccttccctcc ctcaatcggt tgaatgtcgc
     2701 ccttttgtct ttggcgctgg taaaccttac gagttcagta tcgactgcga taagatcaac
     2761 ctgttccgcg gtgtctttgc gtttctttta tatgttgcca cctttatgta tgtattttct
     2821 acgtttgcta acatactgcg taataaggag tcttaatgaa atttggaaac tttttgctta
     2881 cataccaacc tccccaattt tcccaaacag aggtaatgaa acgtttggtt aaattaggtc
     2941 gcatctctga ggagtgtggt tttgataccg tatggttact ggagcatcat ttcacggagt
     3001 ttggtttgct tggtaaccct tatgtcgctg ctgcatattt acttggcgcg actaaaaaat
     3061 tgaatgtagg aactgccgct attgttcttc ccacagccca tccagtacgc caacttgaag
     3121 atgtgaattt attggatcaa atgtcaaaag gacgatttcg gtttggtatt tgccgagggc
     3181 tttacaacaa ggactttcgc gtattcggca cagatatgaa taacagtcgc gccttagcgg
     3241 aatgctggta cgggctgata aagaatggca tgacagaggg atatatggaa gctgataatg
     3301 aacatatcaa gttccataag gtaaaagtaa accccgcggc gtatagcaga ggtggcgcac
     3361 cggtttatgt ggtggctgaa tcagcttcga cgactgagtg ggctgctcaa tttggcctac
     3421 cgatgatatt aagttggatt ataaatacta acgaaaagaa agcacaactt gagctttata
     3481 atgaagtggc tcaagaatat gggcacgata ttcataatat cgaccattgc ttatcatata
     3541 taacatctgt agatcatgac tcaattaaag cgaaagagat ttgccggaaa tttctggggc
     3601 attggtatga ttcttatgtg aatgctacga ctatttttga tgattcagac caaacaagag
     3661 gttatgattt caataaaggg cagtggcgtg actttgtatt aaaaggacat aaagatacta
     3721 atcgccgtat tgattacagt tacgaaatca atcccgtggg aacgccgcag gaatgtattg
     3781 acataattca aaaagacatt gatgctacag gaatatcaaa tatttgttgt ggatttgaag
     3841 ctaatggaac agtagacgaa attattgctt ccatgaagct cttccagtct gatgtcatgc
     3901 catttcttaa agaaaaacaa cgttcgctat tatattatgg cggtggcggt agcggcggtg
     3961 gcggtagcgg cggtggcggt agcggcggtg gcggtagcaa atttggattg ttcttcctta
     4021 acttcatcaa ttcaacaact gttcaagaac agagtatagt tcgcatgcag gaaataacgg
     4081 agtatgttga taagttgaat tttgaacaga ttttagtgta tgaaaatcat ttttcagata
     4141 atggtgttgt cggcgctcct ctgactgttt ctggttttct gctcggttta acagagaaaa
     4201 ttaaaattgg ttcattaaat cacatcatta caactcatca tcctgtccgc atagcggagg
     4261 aagcttgctt attggatcag ttaagtgaag ggagatttat tttagggttt agtgattgcg
     4321 aaaaaaaaga tgaaatgcat ttttttaatc gcccggttga atatcaacag caactatttg
     4381 aagagtgtta tgaaatcatt aacgatgctt taacaacagg ctattgtaat ccagataacg
     4441 atttttatag cttccctaaa atatctgtaa atccccatgc ttatacgcca ggcggacctc
     4501 ggaaatatgt aacagcaacc agtcatcata ttgttgagtg ggcggccaaa aaaggtattc
     4561 ctctcatctt taagtgggat gattctaatg atgttagata tgaatatgct gaaagatata
     4621 aagccgttgc ggataaatat gacgttgacc tatcagagat agaccatcag ttaatgatat
     4681 tagttaacta taacgaagat agtaataaag ctaaacaaga gacgcgtgca tttattagtg
     4741 attatgttct tgaaatgcac cctaatgaaa atttcgaaaa taaacttgaa gaaataattg
     4801 cagaaaacgc tgtcggaaat tatacggagt gtataactgc ggctaagttg gcaattgaaa
     4861 agtgtggtgc gaaaagtgta ttgctgtcct ttgaaccaat gaatgatttg atgagccaaa
     4921 aaaatgtaat caatattgtt gatgataata ttaagaagta ccacacggaa tatacctaaa
     4981 cggagccaat gtacgcaaaa aaccccgctt cagcggggtt ttttcgccaa aaaaaacccc
     5041 gccctgtcag gggcggggtt tttttttagg gccctacgtt cgatgcttct ttgagcgaac
     5101 gatcaaaaat aagtgccttc ccatcaaaaa aatattctca acataaaaaa ctttgtgtaa
     5161 tacttgtaac gctacatgga gattaactca atctagaggg taactagtat gaataataag
     5221 ccgctggatg ttctgatctc tgcgaccggt ctgtggatgt cccgtaccgg cacgctgcac
     5281 aagatcaagc actatgaggt ttctcgttct aaaatctaca tcgaaatggc gtgtggtgac
     5341 catctggttg tgaacaactc tcgctcttgc cgtcccgcac gtgcattccg ttatcataaa
     5401 taccgtaaaa cctgcaaacg ttgtcgtgtt tctgacgaag atatcaacaa cttcctgacc
     5461 cgttctaccg aaggcaaaac ctctgttaaa gttaaagttg tttctgcgcc gaaagtgaaa
     5521 aaagcgatgc cgaaatctgt ttctcgtgcg ccgaaaccgc tgaaaaatcc ggtttctgcg
     5581 aaagcgtcta ccgacacctc tcgttctgtt ccgtctccgg cgaaatctac cccgaactct
     5641 ccggttccga cctctgcaag tgcccccgca cttacgaaga gccagactga caggcttgaa
     5701 gtcctgttaa acccaaaaga tgagatttcc ctgaattccg gcaagccttt cagggagctt
     5761 gagtccgaat tgctctctcg cagaaaaaaa gacctgcagc agatctacgc ggaagaaagg
     5821 gagaattatc tggggaaact cgagcgtgaa attaccaggt tctttgtgga caggggtttt
     5881 ctggaaataa aatccccgat cctgatccct cttgagtata tcgaaaggat gggcattgat
     5941 aatgataccg aactttcaaa acagatcttc agggttgacc agaacttctg cctgagaccc
     6001 atgcttgctc caaaccttta caactacctg cgcaagcttg acagggccct gcctgatcca
     6061 ataaaaattt ttgaaatagg cccatgctac agaaaagagt ccgacggcaa agaacacctc
     6121 gaagagttta ccatgctgaa cttctgccag atgggatcgg gatgcacacg ggaaaatctt
     6181 gaaagcataa ttacggactt cctgaaccac ctgggaattg atttcaagat cgtaggcgat
     6241 tcctgcatgg tctttgggga tacccttgat gtaatgcacg gagacctgga actttcctct
     6301 gcagtagtcg gacccatacc gcttgaccgg gaatggggta ttgataaacc ctggataggg
     6361 gcaggtttcg gactcgaacg ccttctaaag gttaaacacg actttaaaaa tatcaagaga
     6421 gctgcaaggt ccgagtctta ctataacggg atttctacca acctgtaacc taggctgctg
     6481 ccaccgctga gatctatggc ccgcgcccac aggaaggagc taccggcagc ggtgcggact
     6541 gttgtaactc ataataagaa atgaggccgc tcatggcgtt ctgttgcccg tctcgctggt
     6601 gaaaagaaaa accaccctgg ctaactaagc ggcctgctga ctttctcgcc gatcaaatga
     6661 ctgtccgtgt gcttctcaaa tgcctgaggc cagtttgctc aggctctccc cgtggaggta
     6721 ataattgacg atatgatcag tgcacggcta actaagcggc ctgctgactt tctcgccgat
     6781 caaaaggcat tttgctatta agggattgac gagggcgtat ctgcgcagta agatgcgccc
     6841 cgcattcgga aacctgatca tgtagatcga atggactcta aatccgttca gccgggttag
     6901 attcccgggg tttccgccag gatccgtcga cctgcagatc cttagcgaaa gctaaggatt
     6961 ttttttaagc ttctgccatt catccgctta ttatcactta ttcaggcgta gcaccaggcg
     7021 tttaagggca ccaataactg ccttaaaaaa attacgcccc gccctgccac tcatcgcagt
     7081 actgttgtaa ttcattaagc attctgccga cgttcatcgg atcccgggcc cgtcgactgc
     7141 agaggcctgc atgcaagctt ggcgtaatca tgatggactt aattaacggc actcctcagc
     7201 aaatataatg accctcttga taacccaaga gggcattttt taatgcccat ggcgtttatt
     7261 tgccgactac cttggtgatc tcgcctttca cgtagtggac aaattcttcc aactgatctg
     7321 cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct tcaagtatga
     7381 cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg
     7441 cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct
     7501 catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa
     7561 atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa
     7621 cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct
     7681 cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag
     7741 ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga
     7801 gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc
     7861 gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgtggct
     7921 tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat
     7981 ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt
     8041 ccctcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8101 gcggatacat atttgaatgt atttagaaaa atattaattg cgttgcgctc actgcccgct
     8161 ttccaggcca aataggccgt
//
