LOCUS       Exported                4062 bp DNA     circular SYN 15-AUG-2019
DEFINITION  pBAD/His A.
ACCESSION   urn.local...j1-au4wirb
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 4, 2021 from SnapGene 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    4..19
                     /label=araO2
     misc_feature    161..172
                     /label=araO1
     misc_feature    203..216
                     /label=CAP_BS
     misc_feature    208..227
                     /label=pBAD_fwd_primer
     misc_feature    213..251
                     /label=AraI1I2
     misc_feature    239..244
                     /label=BamHI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^GATCC"
     misc_feature    243..269
                     /label=pBAD-seq-F
                     /label=nonstandard type: motif
     misc_feature    248..276
                     /label=ARA_promoter
                     /note="Geneious type: promoter prokaryotic"
     misc_feature    286
                     /label=Txn start site
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     rep_origin      complement(299..399)
                     /direction=LEFT
                     /label=845
                     /note="/created_by=User"
     rep_origin      301..404
                     /label=844
                     /note="/created_by=User"
     misc_feature    305..311
                     /label=RBS
                     /label=nonstandard type: motif
                     /note="/created_by=User"
                     /note="/modified_by=User"
     rep_origin      318..319
                     /label=Excised sfGFP
                     /note="/created_by=User"
     misc_feature    318^319
                     /label=ATGAGCAAGGGCGAAGAACTGTTTACGGGCGTGGTGCCGATTCTGGT
                     /label=ATGAGCAAGGGCGAAGAACTGTTTACGGGCGTGGTGCCGATTCTGGTGGA 
                     ACTGGATGGTGATGTCAATGGTCACAAATTCAGCGTGCGCGGCGAAGGTGAAGGCGAT 
                     GCAACCAATGGTAAACTGACGCTGAAGTTTATTTGCACCACGGGTAAACTGCCGGTTC 
                     CGTGGCCGACCCTGGTCACCACGCTGACGTATGGTGTTCAGTGTTTCAGTCGTTACCC 
                     GGATCACATGAAACGCCACGACTTTTTCAAGTCCGCGATGCCGGAAGGTTATGTCCAA 
                     GAACGTACCATCTCATTTAAAGATGACGGCACCTACAAAACGCGCGCCGAAGTGAAAT 
                     TCGAAGGTGATACGCTGGTTAACCGTATTGAACTGAAAGGCATCGATTTTAAGGAAGA 
                     CGGTAATATTCTGGGCCATAAACTGGAATATAACTTCAATTCGCACAACGTGTACATC 
                     ACCGCAGATAAGCAGAAGAACGGTATCAAGGCTAACTTCAAGATCCGCCATAATGTGG 
                     AAGATGGCAGCGTTCAACTGGCCGACCACTATCAGCAAAACACCCCGATTGGTGATGG 
                     CCCGGTCCTGCTGCCGGACAATCATTACCTGAGCACGCAGTCTGTGCTGAGTAAAGAT 
                     CCGAACGAAAAGCGTGACCACATGGTCCTGCTGGAATTCGTGACCGCGGCCGGCATCA 
                     CGCACGGTATGGACGAACTGTATAAAGGCTCACATCATCATCATCATCATTGA
                     /note="Geneious type: Editing History Deletion"
                     /note="/Original_Bases=ATGAGCAAGGGCGAAGAACTGTTTACGGGCGTGGTG
                     CCGAT 
                     TCTGGTGGAACTGGATGGTGATGTCAATGGTCACAAATTCAGCGTGCGCGGCGAAGGT 
                     GAAGGCGATGCAACCAATGGTAAACTGACGCTGAAGTTTATTTGCACCACGGGTAAAC 
                     TGCCGGTTCCGTGGCCGACCCTGGTCACCACGCTGACGTATGGTGTTCAGTGTTTCAG 
                     TCGTTACCCGGATCACATGAAACGCCACGACTTTTTCAAGTCCGCGATGCCGGAAGGT 
                     TATGTCCAAGAACGTACCATCTCATTTAAAGATGACGGCACCTACAAAACGCGCGCCG 
                     AAGTGAAATTCGAAGGTGATACGCTGGTTAACCGTATTGAACTGAAAGGCATCGATTT 
                     TAAGGAAGACGGTAATATTCTGGGCCATAAACTGGAATATAACTTCAATTCGCACAAC 
                     GTGTACATCACCGCAGATAAGCAGAAGAACGGTATCAAGGCTAACTTCAAGATCCGCC 
                     ATAATGTGGAAGATGGCAGCGTTCAACTGGCCGACCACTATCAGCAAAACACCCCGAT 
                     TGGTGATGGCCCGGTCCTGCTGCCGGACAATCATTACCTGAGCACGCAGTCTGTGCTG 
                     AGTAAAGATCCGAACGAAAAGCGTGACCACATGGTCCTGCTGGAATTCGTGACCGCGG 
                     CCGGCATCACGCACGGTATGGACGAACTGTATAAAGGCTCACATCATCATCATCATCA 
                     TTGA"
     misc_feature    320..388
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    390..395
                     /label=AvaI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=C^YCGRG"
     misc_feature    390..395
                     /label=XhoI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=C^TCGAG"
     misc_feature    398..403
                     /label=PstI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=CTGCA^G"
     misc_feature    418..423
                     /label=EcoRI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^AATTC"
     misc_feature    425..430
                     /label=HindIII
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=A^AGCTT"
     misc_feature    complement(440..464)
                     /label=pBAD-seq-R
                     /label=nonstandard type: motif
     misc_feature    complement(463..480)
                     /label=pBAD_rev_primer
     misc_feature    complement(463..480)
                     /label=pTrcHis_rev_primer
     terminator      513..670
                     /label=rrnB_terminator
     terminator      636..679
                     /label=rrnB_T1_terminator
     terminator      811..838
                     /label=rrnB_T2_terminator
     misc_feature    879..907
                     /label=AmpR_promoter
                     /note="Geneious type: promoter prokaryotic"
     CDS             949..1809
                     /codon_start=1
                     /label=Ampicillin
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    1064..1069
                     /label=ApaLI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^TGCAC"
     rep_origin      1964..2583
                     /label=pBR322_origin
     misc_feature    2310..2315
                     /label=ApaLI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^TGCAC"
     misc_feature    2808..2813
                     /label=ApaLI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^TGCAC"
     misc_feature    2980..3002
                     /label=pGEX_3_primer
     misc_feature    3065..3070
                     /label=SphI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GCATG^C"
     CDS             complement(join(3158..4062,1..25))
                     /codon_start=1
                     /label=ORF frame 3
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIEA
                     NGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPE
                     AREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGR
                     YSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVC
                     LSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRV
                     FKKCTGASPSEFRAGCEEKVNDVAVKLS"
     misc_feature    complement(3158..4036)
                     /label=AraC
     misc_feature    3216..3221
                     /label=AvaI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=C^YCGRG"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
      301 taacaggagg aattaaccat gattaagaag atggcgactg aagatctggg tattggaact
      361 gcttctatgc tgtatagcat tttgtaaagc tcgagatctg cagctggtac catatgggaa
      421 ttcgaagctt ggctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc
      481 agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc
      541 acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc
      601 tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag
      661 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
      721 cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc
      781 cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttttg
      841 cgtttctaca aactcttttg tttatttttc taaatacatt caaatatgta tccgctcatg
      901 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      961 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     1021 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     1081 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     1141 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     1201 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     1261 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     1321 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1381 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1441 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1501 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1561 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1621 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1681 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1741 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1801 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1861 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1921 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1981 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2041 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2101 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     2161 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2221 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2281 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2341 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     2401 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2461 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2521 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2581 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2641 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2701 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2761 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     2821 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     2881 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     2941 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     3001 ggttttcacc gtcatcaccg aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga
     3061 agcggcatgc ataatgtgcc tgtcaaatgg acgaagcagg gattctgcaa accctatgct
     3121 actccgtcaa gccgtcaatt gtctgattcg ttaccaatta tgacaacttg acggctacat
     3181 cattcacttt ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt
     3241 taaatacccg cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga
     3301 taggcatccg ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc
     3361 agcttaagac gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca
     3421 agcaaacatg ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga
     3481 tgtactgaca agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg
     3541 cttccatgcg ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc
     3601 gcccttcccc ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt
     3661 gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat tgacggccag ttaagccatt
     3721 catgccagta ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg
     3781 gatgacgacc gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc
     3841 aaacaaattc tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa
     3901 tataaccttt cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa
     3961 tcggcgttaa acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat
     4021 tttgcgcttc agccatactt ttcatactcc cgccattcag ag
//
