LOCUS       Exported                6993 bp DNA     circular SYN 15-AUG-2019
DEFINITION  pBAD/His A.
ACCESSION   urn.local...j2-au4wirb
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 4, 2021 from SnapGene 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6993
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    4..19
                     /label=araO2
     misc_feature    161..172
                     /label=araO1
     misc_feature    203..216
                     /label=CAP_BS
     misc_feature    208..227
                     /label=pBAD_fwd_primer
     misc_feature    213..251
                     /label=AraI1I2
     misc_feature    239..244
                     /label=BamHI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^GATCC"
     misc_feature    243..269
                     /label=pBAD-seq-F
                     /label=nonstandard type: motif
     misc_feature    248..276
                     /label=ARA_promoter
                     /note="Geneious type: promoter prokaryotic"
     misc_feature    286
                     /label=Txn start site
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     rep_origin      complement(289..338)
                     /direction=LEFT
                     /label=846
                     /note="/created_by=User"
     misc_feature    305..311
                     /label=RBS
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     rep_origin      309..349
                     /label=851
                     /note="/created_by=User"
     rep_origin      319..3318
                     /label=Untagged Parent
                     /note="/created_by=User"
     misc_feature    319..1371
                     /label=pylB-opt 0.70
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    323..3317
                     /label=GCAAGGGCGAAGAACTGTTTACGGGCGTGGTGCCGATTCTGGTGGAA
                     /label=GCAAGGGCGAAGAACTGTTTACGGGCGTGGTGCCGATTCTGGTGGAACTG 
                     GATGGTGATGTCAATGGTCACAAATTCAGCGTGCGCGGCGAAGGTGAAGGCGATGCAA 
                     CCAATGGTAAACTGACGCTGAAGTTTATTTGCACCACGGGTAAACTGCCGGTTCCGTG 
                     GCCGACCCTGGTCACCACGCTGACGTATGGTGTTCAGTGTTTCAGTCGTTACCCGGAT 
                     CACATGAAACGCCACGACTTTTTCAAGTCCGCGATGCCGGAAGGTTATGTCCAAGAAC 
                     GTACCATCTCATTTAAAGATGACGGCACCTACAAAACGCGCGCCGAAGTGAAATTCGA 
                     AGGTGATACGCTGGTTAACCGTATTGAACTGAAAGGCATCGATTTTAAGGAAGACGGT 
                     AATATTCTGGGCCATAAACTGGAATATAACTTCAATTCGCACAACGTGTACATCACCG 
                     CAGATAAGCAGAAGAACGGTATCAAGGCTAACTTCAAGATCCGCCATAATGTGGAAGA 
                     TGGCAGCGTTCAACTGGCCGACCACTATCAGCAAAACACCCCGATTGGTGATGGCCCG 
                     GTCCTGCTGCCGGACAATCATTACCTGAGCACGCAGTCTGTGCTGAGTAAAGATCCGA 
                     ACGAAAAGCGTGACCACATGGTCCTGCTGGAATTCGTGACCGCGGCCGGCATCACGCA 
                     CGGTATGGACGAACTGTATAAAGGCTCACATCATCATCATCATCATTG
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=GCAAGGGCGAAGAACTGTTTACGGGCGTGGTGCCGA
                     TTCTG 
                     GTGGAACTGGATGGTGATGTCAATGGTCACAAATTCAGCGTGCGCGGCGAAGGTGAAG 
                     GCGATGCAACCAATGGTAAACTGACGCTGAAGTTTATTTGCACCACGGGTAAACTGCC 
                     GGTTCCGTGGCCGACCCTGGTCACCACGCTGACGTATGGTGTTCAGTGTTTCAGTCGT 
                     TACCCGGATCACATGAAACGCCACGACTTTTTCAAGTCCGCGATGCCGGAAGGTTATG 
                     TCCAAGAACGTACCATCTCATTTAAAGATGACGGCACCTACAAAACGCGCGCCGAAGT 
                     GAAATTCGAAGGTGATACGCTGGTTAACCGTATTGAACTGAAAGGCATCGATTTTAAG 
                     GAAGACGGTAATATTCTGGGCCATAAACTGGAATATAACTTCAATTCGCACAACGTGT 
                     ACATCACCGCAGATAAGCAGAAGAACGGTATCAAGGCTAACTTCAAGATCCGCCATAA 
                     TGTGGAAGATGGCAGCGTTCAACTGGCCGACCACTATCAGCAAAACACCCCGATTGGT 
                     GATGGCCCGGTCCTGCTGCCGGACAATCATTACCTGAGCACGCAGTCTGTGCTGAGTA 
                     AAGATCCGAACGAAAAGCGTGACCACATGGTCCTGCTGGAATTCGTGACCGCGGCCGG 
                     CATCACGCACGGTATGGACGAACTGTATAAAGGCTCACATCATCATCATCATCATTG"
     misc_feature    1017..1041
                     /label=382
                     /note="/created_by=User"
     misc_feature    1373..2464
                     /label=pylC-opt 0.69
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    complement(1486..1512)
                     /label=450
                     /note="/created_by=User"
     misc_feature    complement(1554..1580)
                     /label=451
                     /note="/created_by=User"
     misc_feature    complement(1746..1770)
                     /label=388
                     /note="/created_by=User"
     misc_feature    1846..1871
                     /label=389
                     /note="/created_by=User"
     misc_feature    2539..3318
                     /label=pylD-opt 0.67
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    complement(2543..2567)
                     /label=383
                     /note="/created_by=User"
     misc_feature    complement(3286..3318)
                     /label=759
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    3289..3342
                     /label=846
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    3321..3326
                     /label=AvaI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=C^YCGRG"
     misc_feature    3321..3326
                     /label=XhoI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=C^TCGAG"
     misc_feature    3329..3334
                     /label=PstI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=CTGCA^G"
     misc_feature    3349..3354
                     /label=EcoRI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^AATTC"
     misc_feature    3356..3361
                     /label=HindIII
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=A^AGCTT"
     misc_feature    complement(3371..3395)
                     /label=pBAD-seq-R
                     /label=nonstandard type: motif
     misc_feature    complement(3394..3411)
                     /label=pBAD_rev_primer
     misc_feature    complement(3394..3411)
                     /label=pTrcHis_rev_primer
     terminator      3444..3601
                     /label=rrnB_terminator
     terminator      3567..3610
                     /label=rrnB_T1_terminator
     terminator      3742..3769
                     /label=rrnB_T2_terminator
     misc_feature    3810..3838
                     /label=AmpR_promoter
                     /note="Geneious type: promoter prokaryotic"
     CDS             3880..4740
                     /codon_start=1
                     /label=Ampicillin
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    3995..4000
                     /label=ApaLI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^TGCAC"
     rep_origin      4895..5514
                     /label=pBR322_origin
     misc_feature    5241..5246
                     /label=ApaLI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^TGCAC"
     misc_feature    5739..5744
                     /label=ApaLI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^TGCAC"
     misc_feature    5911..5933
                     /label=pGEX_3_primer
     misc_feature    5996..6001
                     /label=SphI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GCATG^C"
     CDS             complement(join(6089..6993,1..25))
                     /codon_start=1
                     /label=ORF frame 3
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIEA
                     NGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPE
                     AREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGR
                     YSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVC
                     LSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRV
                     FKKCTGASPSEFRAGCEEKVNDVAVKLS"
     misc_feature    complement(6089..6967)
                     /label=AraC
     misc_feature    6147..6152
                     /label=AvaI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=C^YCGRG"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
      301 taacaggagg aattaaccat gattaagaag atggcgactg aagatctgga tcgttttggc
      361 gaaaaaatta ttgaaggatt tcagttatct gacgacgatt tacgcgcttt gcttagcctg
      421 gaatcggaag aagaattgga aaagctgtat tacgtcgcac gtaaagtacg caaccattac
      481 tttggaaatc gcgtattcct gaactgtttt atctacttca gcacatactg caaaaaccaa
      541 tgtgcgtttt gctattataa ctgcaaaaac gagattaacc gctatcgttt gtcccgcgaa
      601 gaaatcaaag agacttgcaa ggcccttaaa ggtgcgggtt tccatatgat tgatctgact
      661 atgggggagg atccctatta ttacgaggaa cctgatcgct tcgtggagct ggtccaaatg
      721 gttaagaatg agcttggact tccaatcatg atttcgccag gagtaatgga taatgctacc
      781 cttttaaaag cacgcgaaaa gggagctaat ttcttcgcct tatatcaaga aacctatgat
      841 caagagttgt acgagaagtt gcgcgtaggt cagtcgttca aggggcgcta caatactcgt
      901 cgttttgcta aagaacaagg gtattgcatt gaagacggta tcttaacggg agtcgggaac
      961 gatatcgagt caacgatcaa atctttacgc ggcatgaaat caaatgatcc cgacatggtt
     1021 cgcgtgatga ctttcttacc ccaggaagga actccccttg agcgttttaa ggaaaatagt
     1081 aacctgagcg aactgaaaat cattgcgatc ttgcgtctga tctttccgaa gtgcttaatc
     1141 cccgcgtcgt tggacctgga agggatcgac gggatggtac accgtcttaa cgcaggtgct
     1201 aatatcgtga ctagcatcct tcctagtgat tcaaaacttg agggagttgc taactacgat
     1261 cgtgacctgg aagagcgcga ccgcgacatt aaaagcgtca tcaagcgcct tgagggaatg
     1321 ggcatggaac cagcgccaca ggcggaattc gagactgtac tgcgctgcta acatgaaaac
     1381 catctgcctg attggtggta aattgcaggg ttttgaggcc gcgtatctgt ctaaaaaagc
     1441 cgatatgaaa gtagtggtta tcgacaagaa cccccaagct cttatccgca attacgcaga
     1501 cgagtttcat tgttttgatg ttatcaagga accagagaaa cttcttgaaa tcagcaaaaa
     1561 tgtagacgct atcttgcccg tcaacgaaaa cttagaatgt attaaattct taagtagtgt
     1621 aaaagaggaa ttttcttgcc cagtactgtt cgattttgag gcttaccgca tctcgcgcga
     1681 taagaagaaa tcgaaagagt atttccgttc catcggcatt ccgacaccgc aagacaaacc
     1741 tggtcgtcct ccatacttcg ttaagccacc gtgcgaaagc tcgtcggttg gtgctcgcat
     1801 tatctacgat gaagaagaac tgggagaatt ggagcccggc atgctggtag aagagtatgt
     1861 ggaaggtgag gttatttcac ttgaagtaat cggggacggt acccactttg cagttgttaa
     1921 agagacactt attcacatcg accgcaccta tgactgccat atggtaactc ccctgccatc
     1981 tgatttgtct ttccgtgaga tcagctattc tttagcggcc aacctgcctt tgaaggggat
     2041 catggacgtt gaagcaattt cgggcccgca aggtttgaag gtcattgaaa ttgattcacg
     2101 tttcccctcc cagaccccca cagccgtgta ttattcgtcg ggagtgaatt taattgagct
     2161 gttgtttcgt gcctttggag aaggggtgga ggaagttaaa acccttccag aggaccgcta
     2221 ctgtatctac gagcacctga tgcttgcaga aaatggggcc ctgatcccag taggtgagca
     2281 ggttctgtcg atggggaatg actatgggaa atactatgaa gaaccaggaa ttgaaatctt
     2341 tttatgtcgc ggagaggacc ccgtgtttac gcttgtcttt tggggaaagg accgtgagga
     2401 agccgaaaac aaaaagcgca ctggtttgct gattttgaag gaccgcttcg gggcagccgt
     2461 ttaaaatggc aaaaatcgga taagaataaa aatttgccca aagtaaaatt tgtttaaaac
     2521 agaaaaagga gataaaaaat ggctttgttg actcccgagg acctggagaa cattaaccgt
     2581 caacttcacg aagccgattc tacggtacgt cgtgtgactg ggttagatat caagggagtt
     2641 tgtaaagatt tctatgggac tactccatgc tgtgagaagg tcggtatcgt cccggtaact
     2701 agtggcaacg gcatcattgg caacttttct gaaagtcttc gtgcgatcac tgagtacttt
     2761 ggatttgact cattcgtgac gaccatgcca gacgttagtg gctactatga ggcggtccgt
     2821 aatggagcac aaattattct tatggcagat gataatacgt ttcttgcaca caatttaaag
     2881 aacggcaaga tcgctaataa tcaaccgtgt accggaatta tctacgcgga gatcgcctcg
     2941 cgctatatga aagccgattc caaagatgtg cttgccgtcg ggcttggaaa agtggggttt
     3001 ccaggcgctg cacatttggt taataagggg tttcgtgtgt atgggtacga cgcggataag
     3061 gcgttgttag aaaagactgt atcagacctt gggatcacac ctttcgagcc tgagaatccc
     3121 cgtcgcttct caatcatttt tgaggcgacc ccgtgtgctg atacagtacc agagtctgtc
     3181 atcagcgaca attgtgtcat ctctacgccc ggtatccctt gcgcgatcag ccgtgaactt
     3241 caagaaaaat acggggtgga gttgattatg gaacctctgg gtattggaac tgcttctatg
     3301 ctgtatagca ttttgtaaag ctcgagatct gcagctggta ccatatggga attcgaagct
     3361 tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag
     3421 aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc
     3481 catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc
     3541 gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     3601 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag
     3661 cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa
     3721 ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac
     3781 aaactctttt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     3841 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     3901 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     3961 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4021 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4081 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     4141 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4201 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4261 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4321 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4381 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     4441 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     4501 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     4561 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     4621 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     4681 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     4741 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     4801 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     4861 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     4921 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     4981 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5041 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     5101 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5161 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5221 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5281 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5341 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5401 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5461 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5521 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     5581 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     5641 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     5701 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     5761 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     5821 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     5881 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     5941 cgtcatcacc gaaacgcgcg aggcagcaga tcaattcgcg cgcgaaggcg aagcggcatg
     6001 cataatgtgc ctgtcaaatg gacgaagcag ggattctgca aaccctatgc tactccgtca
     6061 agccgtcaat tgtctgattc gttaccaatt atgacaactt gacggctaca tcattcactt
     6121 tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc
     6181 gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc
     6241 gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga
     6301 cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat
     6361 gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac
     6421 aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc
     6481 gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc
     6541 cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat
     6601 ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt
     6661 aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac
     6721 cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt
     6781 ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt
     6841 tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta
     6901 aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt
     6961 cagccatact tttcatactc ccgccattca gag
//
