LOCUS       Exported                7316 bp DNA     circular SYN 15-AUG-2019
DEFINITION  pBAD/His A.
ACCESSION   urn.local...j7-au4wird
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 4, 2021 from SnapGene 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1684..1695
                     /note="/dnas_title=CE-SP-T7 P314T"
     misc_feature    4..19
                     /label=araO2
     misc_feature    161..172
                     /label=araO1
     misc_feature    203..216
                     /label=CAP_BS
     misc_feature    208..227
                     /label=pBAD_fwd_primer
     misc_feature    213..251
                     /label=AraI1I2
     misc_feature    239..244
                     /label=BamHI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^GATCC"
     misc_feature    243..269
                     /label=pBAD-seq-F
                     /label=nonstandard type: motif
     misc_feature    248..276
                     /label=ARA_promoter
                     /note="Geneious type: promoter prokaryotic"
     misc_feature    286
                     /label=Txn start site
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    complement(289..348)
                     /label=761
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     rep_origin      complement(299..3653)
                     /direction=LEFT
                     /label=845
                     /note="/created_by=User"
     rep_origin      301..3658
                     /label=844
                     /note="/created_by=User"
     misc_feature    305..311
                     /label=RBS
                     /label=nonstandard type: motif
                     /note="/created_by=User"
                     /note="/modified_by=User"
     rep_origin      318..3642
                     /label=Excised sfGFP
                     /note="/created_by=User"
     misc_feature    319..1683
                     /label=pylB-opt 0.70
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    319..342
                     /label=758
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    322..633
                     /label=SUMO tag
                     /note="/created_by=User"
     misc_feature    323..3640
                     /label=GCAAGGGCGAAGAACTGTTTACGGGCGTGGTGCCGATTCTGGTGGAA
                     /label=GCAAGGGCGAAGAACTGTTTACGGGCGTGGTGCCGATTCTGGTGGAACTG 
                     GATGGTGATGTCAATGGTCACAAATTCAGCGTGCGCGGCGAAGGTGAAGGCGATGCAA 
                     CCAATGGTAAACTGACGCTGAAGTTTATTTGCACCACGGGTAAACTGCCGGTTCCGTG 
                     GCCGACCCTGGTCACCACGCTGACGTATGGTGTTCAGTGTTTCAGTCGTTACCCGGAT 
                     CACATGAAACGCCACGACTTTTTCAAGTCCGCGATGCCGGAAGGTTATGTCCAAGAAC 
                     GTACCATCTCATTTAAAGATGACGGCACCTACAAAACGCGCGCCGAAGTGAAATTCGA 
                     AGGTGATACGCTGGTTAACCGTATTGAACTGAAAGGCATCGATTTTAAGGAAGACGGT 
                     AATATTCTGGGCCATAAACTGGAATATAACTTCAATTCGCACAACGTGTACATCACCG 
                     CAGATAAGCAGAAGAACGGTATCAAGGCTAACTTCAAGATCCGCCATAATGTGGAAGA 
                     TGGCAGCGTTCAACTGGCCGACCACTATCAGCAAAACACCCCGATTGGTGATGGCCCG 
                     GTCCTGCTGCCGGACAATCATTACCTGAGCACGCAGTCTGTGCTGAGTAAAGATCCGA 
                     ACGAAAAGCGTGACCACATGGTCCTGCTGGAATTCGTGACCGCGGCCGGCATCACGCA 
                     CGGTATGGACGAACTGTATAAAGGCTCACATCATCATCATCATCATTG
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=GCAAGGGCGAAGAACTGTTTACGGGCGTGGTGCCGA
                     TTCTG 
                     GTGGAACTGGATGGTGATGTCAATGGTCACAAATTCAGCGTGCGCGGCGAAGGTGAAG 
                     GCGATGCAACCAATGGTAAACTGACGCTGAAGTTTATTTGCACCACGGGTAAACTGCC 
                     GGTTCCGTGGCCGACCCTGGTCACCACGCTGACGTATGGTGTTCAGTGTTTCAGTCGT 
                     TACCCGGATCACATGAAACGCCACGACTTTTTCAAGTCCGCGATGCCGGAAGGTTATG 
                     TCCAAGAACGTACCATCTCATTTAAAGATGACGGCACCTACAAAACGCGCGCCGAAGT 
                     GAAATTCGAAGGTGATACGCTGGTTAACCGTATTGAACTGAAAGGCATCGATTTTAAG 
                     GAAGACGGTAATATTCTGGGCCATAAACTGGAATATAACTTCAATTCGCACAACGTGT 
                     ACATCACCGCAGATAAGCAGAAGAACGGTATCAAGGCTAACTTCAAGATCCGCCATAA 
                     TGTGGAAGATGGCAGCGTTCAACTGGCCGACCACTATCAGCAAAACACCCCGATTGGT 
                     GATGGCCCGGTCCTGCTGCCGGACAATCATTACCTGAGCACGCAGTCTGTGCTGAGTA 
                     AAGATCCGAACGAAAAGCGTGACCACATGGTCCTGCTGGAATTCGTGACCGCGGCCGG 
                     CATCACGCACGGTATGGACGAACTGTATAAAGGCTCACATCATCATCATCATCATTG"
     misc_feature    complement(605..663)
                     /label=440
                     /note="/created_by=User"
     variation       814..816
                     /label=N61S
                     /label=nonstandard type: mutation
                     /note="/created_by=User"
     variation       883..885
                     /label=E84K
                     /label=nonstandard type: mutation
                     /note="/created_by=User"
     variation       997..999
                     /label=E122K
                     /label=nonstandard type: mutation
                     /note="/created_by=User"
     misc_feature    1156..1158
                     /label=E175K
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    1165..1167
                     /label=E178K
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    1195..1197
                     /label=G188R
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    1329..1353
                     /label=382
                     /note="/created_by=User"
     RBS             1684..1695
                     /label=SD4
                     /note="/created_by=User"
     misc_feature    1684..1695
                     /label=483
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    complement(1684..1695)
                     /label=484
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    1696..2787
                     /label=pylC-opt 0.69
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    complement(2069..2093)
                     /label=388
                     /note="/created_by=User"
     variation       2644..2646
                     /label=E316D
                     /label=nonstandard type: mutation
                     /note="/created_by=User"
     misc_feature    2862..3641
                     /label=pylD-opt 0.67
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    complement(2866..2890)
                     /label=383
                     /note="/created_by=User"
     misc_feature    complement(3609..3641)
                     /label=759
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    3612..3665
                     /label=760
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    3615..3617
                     /label=A252V
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    3644..3649
                     /label=AvaI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=C^YCGRG"
     misc_feature    3644..3649
                     /label=XhoI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=C^TCGAG"
     misc_feature    3652..3657
                     /label=PstI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=CTGCA^G"
     misc_feature    3672..3677
                     /label=EcoRI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^AATTC"
     misc_feature    3679..3684
                     /label=HindIII
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=A^AGCTT"
     misc_feature    complement(3694..3718)
                     /label=pBAD-seq-R
                     /label=nonstandard type: motif
     misc_feature    complement(3717..3734)
                     /label=pBAD_rev_primer
     misc_feature    complement(3717..3734)
                     /label=pTrcHis_rev_primer
     terminator      3767..3924
                     /label=rrnB_terminator
     terminator      3890..3933
                     /label=rrnB_T1_terminator
     terminator      4065..4092
                     /label=rrnB_T2_terminator
     misc_feature    4133..4161
                     /label=AmpR_promoter
                     /note="Geneious type: promoter prokaryotic"
     CDS             4203..5063
                     /label=Ampicillin
     misc_feature    4318..4323
                     /label=ApaLI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^TGCAC"
     rep_origin      5218..5837
                     /label=pBR322_origin
     misc_feature    5564..5569
                     /label=ApaLI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^TGCAC"
     misc_feature    6062..6067
                     /label=ApaLI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^TGCAC"
     misc_feature    6234..6256
                     /label=pGEX_3_primer
     misc_feature    6319..6324
                     /label=SphI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GCATG^C"
     CDS             complement(join(6412..7316,1..25))
                     /label=ORF frame 3
     misc_feature    complement(6412..7290)
                     /label=AraC
     misc_feature    6470..6475
                     /label=AvaI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=C^YCGRG"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
      301 taacaggagg aattaaccat gatgggcagc agcatgtcgg actcagaagt caatcaagaa
      361 gctaagccag aggtcaagcc agaagtcaag cctgagactc acatcaattt aaaggtgtcc
      421 gatgggtctt cagagatctt cttcaagatc aaaaagacca ctcctttaag aaggctgatg
      481 gaagcgttcg ctaaaagaca gggtaaggaa atggactcct taagattctt gtacgacggt
      541 attagaattc aagctgatca gacccctgaa gatttggaca tggaggataa cgatattatt
      601 gaggctcaca gagaacagat tggtggtcat atgattaaga agatggcgac tgaagatctg
      661 gatcgttttg gcgaaaaaat tattgaagga tttcagttat ctgacgacga tttacgcgct
      721 ttgcttagcc tggaatcgga agaagaattg gaaaagctgt attacgtcgc acgtaaagta
      781 cgcaaccatt actttggaaa tcgcgtattc ctgagctgtt ttatctactt cagcacatac
      841 tgcaaaaacc aatgtgcgtt ttgctattat aactgcaaaa acaagattaa ccgctatcgt
      901 ttgtcccgcg aagaaatcaa agagacttgc aaggccctta aaggtgcggg tttccatatg
      961 attgatctga ctatggggga ggatccctat tattacaagg aacctgatcg cttcgtggag
     1021 ctggtccaaa tggttaagaa tgagcttgga cttccaatca tgatttcgcc aggagtaatg
     1081 gataatgcta cccttttaaa agcacgcgaa aagggagcta atttcttcgc cttatatcaa
     1141 gaaacctatg atcaagagtt gtacgagaag ttgcgcgtag gtcagtcgtt caaggggcgc
     1201 tacaatactc gtcgttttgc taaagaacaa gggtattgca ttgaagacgg tatcttaacg
     1261 ggagtcggga acgatatcga gtcaacgatc aaatctttac gcggcatgaa atcaaatgat
     1321 cccgacatgg ttcgcgtgat gactttctta ccccaggaag gaactcccct tgagcgtttt
     1381 aaggaaaata gtaacctgag cgaactgaaa atcattgcga tcttgcgtct gatctttccg
     1441 aagtgcttaa tccccgcgtc gttggacctg gaagggatcg acgggatggt acaccgtctt
     1501 aacgcaggtg ctaatatcgt gactagcatc cttcctagtg attcaaaact tgagggagtt
     1561 gctaactacg atcgtgacct ggaagagcgc gaccgcgaca ttaaaagcgt catcaagcgc
     1621 cttgagggaa tgggcatgga accagcgcca caggcggaat tcgagactgt actgcgctgc
     1681 taataaggag gaaaaatgaa aaccatctgc ctgattggtg gtaaattgca gggttttgag
     1741 gccgcgtatc tgtctaaaaa agccgatatg aaagtagtgg ttatcgacaa gaacccccaa
     1801 gctcttatcc gcaattacgc agacgagttt cattgttttg atgttatcaa ggaaccagag
     1861 aaacttcttg aaatcagcaa aaatgtagac gctatcttgc ccgtcaacga aaacttagaa
     1921 tgtattaaat tcttaagtag tgtaaaagag gaattttctt gcccagtact gttcgatttt
     1981 gaggcttacc gcatctcgcg cgataagaag aaatcgaaag agtatttccg ttccatcggc
     2041 attccgacac cgcaagacaa acctggtcgt cctccatact tcgttaagcc accgtgcgaa
     2101 agctcgtcgg ttggtgctcg cattatctac gatgaagaag aactgggaga attggagccc
     2161 ggcatgctgg tagaagagta tgtggaaggt gaggttattt cacttgaagt aatcggggac
     2221 ggtacccact ttgcagttgt taaagagaca cttattcaca tcgaccgcac ctatgactgc
     2281 catatggtaa ctcccctgcc atctgatttg tctttccgtg agatcagcta ttctttagcg
     2341 gccaacctgc ctttgaaggg gatcatggac gttgaagcaa tttcgggccc gcaaggtttg
     2401 aaggtcattg aaattgattc acgtttcccc tcccagaccc ccacagccgt gtattattcg
     2461 tcgggagtga atttaattga gctgttgttt cgtgcctttg gagaaggggt ggaggaagtt
     2521 aaaacccttc cagaggaccg ctactgtatc tacgagcacc tgatgcttgc agaaaatggg
     2581 gccctgatcc cagtaggtga gcaggttctg tcgatgggga atgactatgg gaaatactat
     2641 gaagatccag gaattgaaat ctttttatgt cgcggagagg accccgtgtt tacgcttgtc
     2701 ttttggggaa aggaccgtga ggaagccgaa aacaaaaagc gcactggttt gctgattttg
     2761 aaggaccgct tcggggcagc cgtttaaaat ggcaaaaatc ggataagaat aaaaatttgc
     2821 ccaaagtaaa atttgtttaa aacagaaaaa ggagataaaa aatggctttg ttgactcccg
     2881 aggacctgga gaacattaac cgtcaacttc acgaagccga ttctacggta cgtcgtgtga
     2941 ctgggttaga tatcaaggga gtttgtaaag atttctatgg gactactcca tgctgtgaga
     3001 aggtcggtat cgtcccggta actagtggca acggcatcat tggcaacttt tctgaaagtc
     3061 ttcgtgcgat cactgagtac tttggatttg actcattcgt gacgaccatg ccagacgtta
     3121 gtggctacta tgaggcggtc cgtaatggag cacaaattat tcttatggca gatgataata
     3181 cgtttcttgc acacaactta aagaacggca agatcgctaa taatcaaccg tgtaccggaa
     3241 ttatctacgc ggagatcgcc tcgcgctata tgaaagccga ttccaaagat gtgcttgccg
     3301 tcgggcttgg aaaagtgggg tttccaggcg ctgcacattt ggttaataag gggtttcgtg
     3361 tgtatgggta cgacgcggat aaggcgttgt tagaaaagac tgtatcagac cttgggatca
     3421 cacctttcga gcctgagaat ccccgtcgct tctcaatcat ttttgaggcg accccgtgtg
     3481 ctgatacagt accagagtct gtcatcagcg acaattgtgt catctctacg cccggtatcc
     3541 cttgcgcgat cagccgtgaa cttcaagaaa aatacggggt ggagttgatt atggaacctc
     3601 tgggtattgg aactgcttct atgctgtata gcattttgta aagctcgaga tctgcagctg
     3661 gtaccatatg ggaattcgaa gcttggctgt tttggcggat gagagaagat tttcagcctg
     3721 atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt
     3781 agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat
     3841 ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa
     3901 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
     3961 gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg
     4021 gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac
     4081 ggatggcctt tttgcgtttc tacaaactct tttgtttatt tttctaaata cattcaaata
     4141 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     4201 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     4261 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     4321 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     4381 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     4441 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     4501 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     4561 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     4621 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     4681 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     4741 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     4801 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     4861 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     4921 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     4981 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     5041 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     5101 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5161 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5221 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5281 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5341 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     5401 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5461 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5521 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5581 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5641 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5701 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5761 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5821 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5881 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     5941 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     6001 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     6061 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
     6121 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     6181 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     6241 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc agatcaattc
     6301 gcgcgcgaag gcgaagcggc atgcataatg tgcctgtcaa atggacgaag cagggattct
     6361 gcaaacccta tgctactccg tcaagccgtc aattgtctga ttcgttacca attatgacaa
     6421 cttgacggct acatcattca ctttttcttc acaaccggca cggaactcgc tcgggctggc
     6481 cccggtgcat tttttaaata cccgcgagaa atagagttga tcgtcaaaac caacattgcg
     6541 accgacggtg gcgataggca tccgggtggt gctcaaaagc agcttcgcct ggctgatacg
     6601 ttggtcctcg cgccagctta agacgctaat ccctaactgc tggcggaaaa gatgtgacag
     6661 acgcgacggc gacaagcaaa catgctgtgc gacgctggcg atatcaaaat tgctgtctgc
     6721 caggtgatcg ctgatgtact gacaagcctc gcgtacccga ttatccatcg gtggatggag
     6781 cgactcgtta atcgcttcca tgcgccgcag taacaattgc tcaagcagat ttatcgccag
     6841 cagctccgaa tagcgccctt ccccttgccc ggcgttaatg atttgcccaa acaggtcgct
     6901 gaaatgcggc tggtgcgctt catccgggcg aaagaacccc gtattggcaa atattgacgg
     6961 ccagttaagc cattcatgcc agtaggcgcg cggacgaaag taaacccact ggtgatacca
     7021 ttcgcgagcc tccggatgac gaccgtagtg atgaatctct cctggcggga acagcaaaat
     7081 atcacccggt cggcaaacaa attctcgtcc ctgatttttc accaccccct gaccgcgaat
     7141 ggtgagattg agaatataac ctttcattcc cagcggtcgg tcgataaaaa aatcgagata
     7201 accgttggcc tcaatcggcg ttaaacccgc caccagatgg gcattaaacg agtatcccgg
     7261 cagcagggga tcattttgcg cttcagccat acttttcata ctcccgccat tcagag
//
