LOCUS       Exported                9983 bp DNA     circular SYN 30-JUL-2019
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...1j-dfav18m
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 4, 2021 from SnapGene 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9983
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     variation       276..300
                     /label=579
                     /label=nonstandard type: mutation
                     /note="/created_by=User"
                     /note="/modified_by=User"
     variation       complement(370..394)
                     /label=580
                     /label=nonstandard type: mutation
                     /note="/created_by=User"
     misc_feature    499..523
                     /label=OVERLAP
                     /note="/Transferred_From=pDB005CH(+), pDB005CHx, 
                     pDB005CHx(-),
                     pDB005x, pDB005x (repaired), pDB021CH(+), pDB021CH(+) - 
                     gblock, pDB040"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     primer_bind     complement(499..523)
                     /label=DB102
                     /note="/Transferred_From=pDB038b"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     primer_bind     complement(499..518)
                     /label=DB60
                     /note="/Transferred_From=pDB027a"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     promoter        555..657
                     /label=lpp promoter
                     /note="color: #800000; direction: RIGHT; lpp promoter"
                     /note="/Transferred_From=pCDF-PS28h"
                     /note="/Transferred_Similarity=100.00%"
     promoter        555..634
                     /label=lpp
                     /note="/Transferred_From=pDB024f, pDB024f2, pDB025, 
                     pDB025a,
                     pDB060, pDB060IF, pDB060IF_IPYE, pDB060Mb, pDB060Mb_tetra, 
                     pDB060Mm, pDB060Mm_tetra, pDB060Y349F, pDB060tetra, 
                     pDB060tetra/349F, pDB063.pIF, pDB063.pNF, 
                     pTechf-PylBCDopt-PylSopt-PylT, 
                     pTechf-PylBCDopt-PylSopt-PylT extraction"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    559..582
                     /label=378
                     /note="/created_by=User"
     misc_feature    571..598
                     /label=Dyad Symmetry
                     /note="/Transferred_From=HHR-plasmid, SP-HHR-tRNA,
                     SP-TheoHHR-tRNA, Theo-plasmid, pDB008b (reversed), pDB008c 
                     (reversed)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    608..654
                     /label=Lpp
                     /note="Lpp; color: #00ff00; direction: LEFT"
                     /note="/Transferred_From=Exported File"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     663..717
                     /label=DB405
     primer_bind     complement(667..693)
                     /label=DB402
     CDS             694..1953
                     /codon_start=1
                     /label=chPylRS
                     /note="/created_by=User"
                     /translation="MDKKPLDVLISATGLWMSRTGTLHKIKHYEVSRSKIYIEMACGDH
                     LVVNNSRSCRTARAFRHHKYRKTCKRCRVSDEDINNFLTRSTEGKTSVKVKVVSAPKVK
                     KAMPKSVSRAPKPLENPVSAKASTDTSRSVPSPAKSTPNSPVPTSASAPALTKSQTDRL
                     EVLLNPKDEISLNSGKPFRELESELLSRRKKDLQQIYAEERENYLGKLEREITRFFVDR
                     GFLEIKSPILIPLEYIERMGIDNDTELSKQIFRVDKNFCLRPMLAPNLYNYLRKLDRAL
                     PDPIKIFEIGPCYRKESDGKEHLEEFTMLNFCQMGSGCTRENLESIITDFLNHLGIDFK
                     IVGDSCMVYGDTLDVMHGDLELSSAVVGPIPLDREWGIDKPWIGAGFGLERLLKVKHDF
                     KNIKRAARSESYYNGISTNL"
     primer_bind     694..717
                     /label=DB133
     primer_bind     complement(694..717)
                     /label=DB270
     primer_bind     892..917
                     /label=DB360
     primer_bind     complement(955..982)
                     /label=DB364
     primer_bind     complement(1120..1138)
                     /label=DB572-u
     primer_bind     complement(1121..1140)
                     /label=DB406
     primer_bind     1121..1139
                     /label=DB569-u
     primer_bind     1146..1165
                     /label=DB413
     primer_bind     1285..1305
                     /label=DB141
     primer_bind     complement(1445..1469)
                     /label=DB445
     primer_bind     complement(1467..1488)
                     /label=DB370
     primer_bind     complement(1516..1577)
                     /label=DB376
     primer_bind     complement(1558..1648)
                     /label=Primer
     primer_bind     complement(1623..1633)
                     /label=DB370
     primer_bind     1636..1735
                     /label=DB377
     primer_bind     complement(1645..1669)
                     /label=DB367
     primer_bind     complement(1663..1688)
                     /label=DB570-u
     primer_bind     1675..1696
                     /label=DB571-u
     primer_bind     1743..1766
                     /label=DB516
     primer_bind     1867..1888
                     /label=DB142
     primer_bind     complement(1918..1953)
                     /label=DB134
     primer_bind     complement(1930..1953)
                     /label=DB268
     primer_bind     1930..1953
                     /label=DB269
     promoter        1954..2204
                     /label=ProK
                     /note="/created_by=User"
     tRNA            2205..2276
                     /label=PylT
                     /label=PylT; pylT
                     /note="/Transferred_From=pDB005CH(+), pDB005CHx, 
                     pDB005CHx(-),
                     pDB005x, pDB005x (repaired), pDB012a (multiple clones need 
                     sequencing), pDB021CH(+), pDB021CH(+) - gblock, 
                     pDB021CHx(-), pDB031a, pDB031e, pDB032, pDB032 - pylT, 
                     pDB032c, pDB032c Copy, pDB032c(–), pDB032d, pDB032e, 
                     pDB032f, pDB032g, pDB032h, pDB037ns2, pDB037ns2a, 
                     pDB037ns3a, pDB037ns3b, pDB037ns3c, pDB037ns3d, pDB037ns4a,
                     pDB037ns4b, pDB037ns4c, pDB038, pDB038a, pDB038b, pDB039, 
                     pDB040, pDB042 (not made), pDB045ns1, pDB045ns1.2 (not 
                     made), pDB046c, pDB046c(–), pDB049, pDB050a (wait to 
                     clone), pDB050b (wait to clone), pDB050c (wait to clone), 
                     pDB050d (wait to clone), pDB053ns2, pDB055a1-from38, 
                     pDB055b1-from38a, pDB056, pDB058a (not made), pDB058b (not 
                     made), pDB058c (not made), pDB060, pDB060IF, pDB060IF_IPYE,
                     pDB060Mb, pDB060Mb_tetra, pDB060Mm, pDB060Mm_tetra, 
                     pDB060Y349F, pDB060tetra, pDB060tetra/349F, pDB061a, 
                     pDB061b, pDB061c, pDB061d, pTechf-PylBCDopt-PylSopt-PylT"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    2205..2276
                     /label=PylT
                     /note="/Transferred_From=pRSFDuet_1"
                     /note="/Transferred_Similarity=100.00%"
     tRNA            2205..2273
                     /label=PylT
                     /note="/Transferred_From=U6-PylT, pCMV-PylRST_iRFP.dib
                     (modified), pCMV-PylRST_mCherry.dib (modified)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    complement(2324..2349)
                     /label=379
                     /note="/created_by=User"
     misc_feature    2324..2349
                     /label=956
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     primer_bind     complement(2328..2349)
                     /label=tRNA_Seq.Forward1
                     /note="/Transferred_From=HHR-plasmid"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    complement(2405..2440)
                     /label=955
                     /label=nonstandard type: motif
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    2411..2451
                     /label=952
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     promoter        2446..2493
                     /label=EM7 promoter
     promoter        2451..2496
                     /label=EM7 promoter, published fragment
     misc_feature    2567..2574
                     /label=RBS (probably)
     misc_feature    2580..4022
                     /label=luxC (ORF1)
     misc_feature    3159..3186
                     /label=958
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    3974..4001
                     /label=959
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    4035..4958
                     /label=luxD (ORF2)
     misc_feature    4764..4791
                     /label=960
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    5007..6089
                     /label=luxA (ORF3)
     misc_feature    5634..5658
                     /label=900
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    complement(6050..6102)
                     /label=976
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    complement(6073..6121)
                     /label=978
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    6087..6089
                     /label=GOOD luxA term. TAA
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    6089
                     /label=G
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=G"
     misc_feature    6095..6144
                     /label=977
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    6104..7090
                     /label=luxB (ORF4)
     misc_feature    6107..6109
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    6107..6109
                     /label=1TAG
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    6378..6405
                     /label=901
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    7164..7188
                     /label=961
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    7269..8381
                     /label=luxE(ORF5)
     misc_feature    7853..7883
                     /label=962
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    complement(8541..8576)
                     /label=953
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    8551..8592
                     /label=954
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    complement(8618..8644)
                     /label=957
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     rep_origin      8697..9609
                     /label=p15A origin
                     /note="/Transferred_From=pTechf-PylBCDopt-PylSopt-PylT"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     primer_bind     complement(9040..9059)
                     /label=tRNA_Seq.Forward3
                     /note="/Transferred_From=HHR-plasmid"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     primer_bind     complement(9041..9059)
                     /label=Seq.Forward13
                     /note="/Transferred_From=pDB038b"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     primer_bind     complement(9376..9396)
                     /label=DB539
     promoter        complement(9673..9764)
                     /label=Promoter_P_1
                     /label=Promoter_P_1; cat promoter
                     /note="cat promoter; from pACYCDuet-1"
                     /note="/Transferred_From=pAB081j, pJC166d - T7 RNAP redon"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/modified_by=User"
     CDS             join(9765..9983,1..233)
                     /label=CamR
                     /note="/Transferred_From=pTechf-PylBCDopt-PylSopt-PylT"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     primer_bind     complement(9814..9835)
                     /label=DB538
ORIGIN
        1 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
       61 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
      121 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggcgaaaa cctggcctat
      181 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
      241 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
      301 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
      361 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
      421 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt
      481 tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgaa agcaaattcg
      541 acccgggcgg ccgcaaccca gcgttcgatg cttctttgag cgaacgatca aaaataagtg
      601 ccttcccatc aaaaaaatat tctcaacata aaaaactttg tgtaatactt gtaacgctac
      661 atggagatta actcaatcta gagggtaact agtatggata agaagccgct ggatgttctg
      721 atctctgcga ccggtctgtg gatgtcccgt accggcacgc tgcacaagat caagcactat
      781 gaggtttctc gttctaaaat ctacatcgaa atggcgtgtg gtgaccatct ggttgtgaac
      841 aactctcgtt cttgtcgtac cgcacgtgca ttccgtcatc ataaataccg taaaacctgc
      901 aaacgttgtc gtgtttctga cgaagatatc aacaacttcc tgacccgttc taccgaaggc
      961 aaaacctctg ttaaagttaa agttgtttct gcgccgaaag tgaaaaaagc gatgccgaaa
     1021 tctgtttctc gtgcgccgaa accgctggaa aatccggttt ctgcgaaagc gtctaccgac
     1081 acctctcgtt ctgttccgtc tccggcgaaa tctaccccga actctccggt tccgacctct
     1141 gcaagtgccc ccgcacttac gaagagccag actgacaggc ttgaagtcct gttaaaccca
     1201 aaagatgaga tttccctgaa ttccggcaag cctttcaggg agcttgagtc cgaattgctc
     1261 tctcgcagaa aaaaagacct gcagcagatc tacgcggaag aaagggagaa ttatctgggg
     1321 aaactcgagc gtgaaattac caggttcttt gtggacaggg gttttctgga aataaaatcc
     1381 ccgatcctga tccctcttga gtatatcgaa aggatgggca ttgataatga taccgaactt
     1441 tcaaaacaga tcttcagggt tgacaagaac ttctgcctga gacccatgct tgctccaaac
     1501 ctttacaact acctgcgcaa gcttgacagg gccctgcctg atccaataaa aatttttgaa
     1561 ataggcccat gctacagaaa agagtccgac ggcaaagaac acctcgaaga gtttaccatg
     1621 ctgaacttct gccagatggg atcgggatgc acacgggaaa atcttgaaag cataattacg
     1681 gacttcctga accacctggg aattgatttc aagatcgtag gcgattcctg catggtctat
     1741 ggggataccc ttgatgtaat gcacggagac ctggaacttt cctctgcagt agtcggaccc
     1801 ataccgcttg accgggaatg gggtattgat aaaccctgga taggggcagg tttcgggctc
     1861 gaacgccttc taaaggttaa acacgacttt aaaaatatca agagagctgc aaggtccgag
     1921 tcttactata acgggatttc taccaacctg taacctaggc tgctgccacc gctgagatct
     1981 atggcccgcg cccacaggaa ggagctaccg gcagcggtgc ggactgttgt aactcataat
     2041 aagaaatgag gccgctcatg gcgttctgtt gcccgtctcg ctggtgaaaa gaaaaaccac
     2101 cctggctaac taagcggcct gctgactttc tcgccgatca aaaggcattt tgctattaag
     2161 ggattgacga gggcgtatct gcgcagtaag atgcgccccg cattggaaac ctgatcatgt
     2221 agatcgaatg gactctaaat ccgttcagcc gggttagatt cccggggttt ccgccaaatt
     2281 cgaaaagcct gctcaacgag caggcttttt tgcatggcgt tacccaactt aatcgccttg
     2341 cagcacatcc ccctttcgcc agatcccgca agaggcccgg cagtaccggc ataaccaagc
     2401 ctatgcctac agcatccagg gtgacgcgtt taaacccatg gacgtgttga caattaatca
     2461 tcggcatagt atatcggcat agtataatac gactcagggc ccactagtgg tacccgggga
     2521 tcctctagag tcgacctgca ggtcgacgga tccggggaat tcaggcttgg aggatacgta
     2581 tgactaaaaa aatttcattc attattaacg gccaggttga aatctttccc gaaagtgatg
     2641 atttagtgca atccattaat tttggtgata atagtgttta cctgccaata ttgaatgact
     2701 ctcatgtaaa aaacattatt gattgtaatg gaaataacga attacggttg cataacattg
     2761 tcaattttct ctatacggta gggcaaagat ggaaaaatga agaatactca agacgcagga
     2821 catacattcg tgacttaaaa aaatatatgg gatattcaga agaaatggct aagctagagg
     2881 ccaattggat atctatgatt ttatgttcta aaggcggcct ttatgatgtt gtagaaaatg
     2941 aacttggttc tcgccatatc atggatgaat ggctacctca ggatgaaagt tatgttcggg
     3001 cttttccgaa aggtaaatct gtacatctgt tggcaggtaa tgttccatta tctgggatca
     3061 tgtctatatt acgcgcaatt ttaactaaga atcagtgtat tataaaaaca tcgtcaaccg
     3121 atccttttac cgctaatgca ttagcgttaa gttttattga tgtagaccct aatcatccga
     3181 taacgcgctc tttatctgtt atatattggc cccaccaagg tgatacatca ctcgcaaaag
     3241 aaattatgcg acatgcggat gttattgtcg cttggggagg gccagatgcg attaattggg
     3301 cggtagagca tgcgccatct tatgctgatg tgattaaatt tggttctaaa aagagtcttt
     3361 gcattatcga taatcctgtt gatttgacgt ccgcagcgac aggtgcggct catgatgttt
     3421 gtttttacga tcagcgagct tgtttttctg cccaaaacat atattacatg ggaaatcatt
     3481 atgaggaatt taagttagcg ttgatagaaa aacttaatct atatgcgcat atattaccga
     3541 atgccaaaaa agattttgat gaaaaggcgg cctattcttt agttcaaaaa gaaagcttgt
     3601 ttgctggatt aaaagtagag gtggatattc atcaacgttg gatgattatt gagtcaaatg
     3661 caggtgtgga atttaatcaa ccacttggca gatgtgtgta ccttcatcac gtcgataata
     3721 ttgagcaaat attgccttat gttcaaaaaa ataagacgca aaccatatct atttttcctt
     3781 gggagtcatc atttaaatat cgagatgcgt tagcattaaa aggtgcggaa aggattgtag
     3841 aagcaggaat gaataacata tttcgagttg gtggatctca tgacggaatg agaccgttgc
     3901 aacgattagt gacatatatt tctcatgaaa ggccatctaa ctatacggct aaggatgttg
     3961 cggttgaaat agaacagact cgattcctgg aagaagataa gttccttgta tttgtcccat
     4021 aataggtaaa aagtatggaa aatgaatcaa aatataaaac catcgaccac gttatttgtg
     4081 ttgaaggaaa taaaaaaatt catgtttggg aaacgctgcc agaagaaaac agcccaaaga
     4141 gaaagaatgc cattattatt gcgtctggtt ttgcccgcag gatggatcat tttgctggtc
     4201 tggcggaata tttatcgcgg aatggatttc atgtgatccg ctatgattcg cttcaccacg
     4261 ttggattgag ttcagggaca attgatgaat ttacaatgtc tataggaaag cagagcttgt
     4321 tagcagtggt tgattggtta actacacgaa aaataaataa cttcggtatg ttggcttcaa
     4381 gcttatctgc gcggatagct tatgcaagcc tatctgaaat caatgcttcg tttttaatca
     4441 ccgcagtcgg tgttgttaac ttaagatatt ctcttgaaag agctttaggg tttgattatc
     4501 tcagtctacc cattaatgaa ttgccggata atctagattt tgaaggccat aaattgggtg
     4561 ctgaagtctt tgcgagagat tgtcttgatt ttggttggga agatttagct tctacaatta
     4621 ataacatgat gtatcttgat ataccgttta ttgcttttac tgcaaataac gataattggg
     4681 tcaagcaaga tgaagttatc acattgttat caaatattcg tagtaatcga tgcaagatat
     4741 attctttgtt aggaagttcg catgacttga gtgaaaattt agtggtcctg cgcaattttt
     4801 atcaatcggt tacgaaagcc gctatcgcga tggataatga tcatctggat attgatgttg
     4861 atattactga accgtcattt gaacatttaa ctattgcgac agtcaatgaa cgccgaatga
     4921 gaattgagat tgaaaatcaa gcaatttctc tgtcttaaaa tctattgaga tattctatca
     4981 ctcaaatagc aatataagga ctctctatga aatttggaaa ctttttgctt acataccaac
     5041 ctccccaatt ttctcaaaca gaggtaatga aacgtttggt taaattaggt cgcatctctg
     5101 aggagtgtgg ttttgatacc gtatggttac tggagcatca tttcacggag tttggtttgc
     5161 ttggtaaccc ttatgtcgct gctgcatatt tacttggcgc gactaaaaaa ttgaatgtag
     5221 gaactgccgc tattgttctt cccacagccc atccagtacg ccaacttgaa gatgtgaatt
     5281 tattggatca aatgtcaaaa ggacgatttc ggtttggtat ttgccgaggg ctttacaaca
     5341 aggactttcg cgtattcggc acagatatga ataacagtcg cgccttagcg gaatgctggt
     5401 acgggctgat aaagaatggc atgacagagg gatatatgga agctgataat gaacatatca
     5461 agttccataa ggtaaaagta aaccccgcgg cgtatagcag aggtggcgca ccggtttatg
     5521 tggtggctga atcagcttcg acgactgagt gggctgctca atttggccta ccgatgatat
     5581 taagttggat tataaatact aacgaaaaga aagcacaact tgagctttat aatgaagtgg
     5641 ctcaagaata tgggcacgat attcataata tcgaccattg cttatcatat ataacatctg
     5701 tagatcatga ctcaattaaa gcgaaagaga tttgccggaa atttctgggg cattggtatg
     5761 attcttatgt gaatgctacg actatttttg atgattcaga ccaaacaaga ggttatgatt
     5821 tcaataaagg gcagtggcgt gactttgtat taaaaggaca taaagatact aatcgccgta
     5881 ttgattacag ttacgaaatc aatcccgtgg gaacgccgca ggaatgtatt gacataattc
     5941 aaaaagacat tgatgctaca ggaatatcaa atatttgttg tggatttgaa gctaatggaa
     6001 cagtagacga aattattgct tccatgaagc tcttccagtc tgatgtcatg ccatttctta
     6061 aagaaaaaca acgttcgcta ttatattaac taaggagaaa gaaatgtaga aatttggatt
     6121 gttcttcctt aacttcatca attcaacaac tgttcaagaa caaagtatag ttcgcatgca
     6181 ggaaataacg gagtatgttg ataagttgaa ttttgaacag attttagtgt atgaaaatca
     6241 tttttcagat aatggtgttg tcggcgctcc tctgactgtt tctggttttc tgctcggttt
     6301 aacagagaaa attaaaattg gttcattaaa tcacatcatt acaactcatc atcctgtcgc
     6361 catagcggag gaagcttgct tattggatca gttaagtgaa gggagattta ttttagggtt
     6421 tagtgattgc gaaaaaaaag atgaaatgca tttttttaat cgcccggttg aatatcaaca
     6481 gcaactattt gaagagtgtt atgaaatcat taacgatgct ttaacaacag gctattgtaa
     6541 tccagataac gatttttata gcttccctaa aatatctgta aatccccatg cttatacgcc
     6601 aggcggacct cggaaatatg taacagcaac cagtcatcat attgttgagt gggcggccaa
     6661 aaaaggtatt cctctcatct ttaagtggga tgattctaat gatgttagat atgaatatgc
     6721 tgaaagatat aaagccgttg cggataaata tgacgttgac ctatcagaga tagaccatca
     6781 gttaatgata ttagttaact ataacgaaga tagtaataaa gctaaacaag agacgcgtgc
     6841 atttattagt gattatgttc ttgaaatgca ccctaatgaa aatttcgaaa ataaacttga
     6901 agaaataatt gcagaaaacg ctgtcggaaa ttatacggag tgtataactg cggctaagtt
     6961 ggcaattgaa aagtgtggtg cgaaaagtgt attgctgtcc tttgaaccaa tgaatgattt
     7021 gatgagccaa aaaaatgtaa tcaatattgt tgatgataat attaagaagt accacatgga
     7081 atatacctaa tagatttcga gttgcagcga ggcggcaagt gaacgaatcc ccaggagcat
     7141 agataactat gtgactgggg tgagtgaaag cagccaacaa agcagcagct tgaaagatga
     7201 agggtataaa agagtatgac agcagtgctg ccatactttc taatattatc ttgaggagta
     7261 aaacaggtat gacttcatat gttgataaac aagaaattac agcaagctca gaaattgatg
     7321 atttgatttt ttcgagcgat ccattagtgt ggtcttacga cgagcaggaa aaaatcagaa
     7381 agaaacttgt gcttgatgca tttcgtaatc attataaaca ttgtcgagaa tatcgtcact
     7441 actgtcaggc acacaaagta gatgacaata ttacggaaat tgatgacata cctgtattcc
     7501 caacatcggt ttttaagttt actcgcttat taacttctca ggaaaacgag attgaaagtt
     7561 ggtttaccag tagcggcacg aatggtttaa aaagtcaggt ggcgcgtgac agattaagta
     7621 ttgagagact cttaggctct gtgagttatg gcatgaaata tgttggtagt tggtttgatc
     7681 atcaaataga attagtcaat ttgggaccag atagatttaa tgctcataat atttggttta
     7741 aatatgttat gagtttggtg gaattgttat atcctacgac atttaccgta acagaagaac
     7801 gaatagattt tgttaaaaca ttgaatagtc ttgaacgaat aaaaaatcaa gggaaagatc
     7861 tttgtcttat tggttcgcca tactttattt atttactctg ccattatatg aaagataaaa
     7921 aaatctcatt ttctggagat aaaagccttt atatcataac cggaggcggc tggaaaagtt
     7981 acgaaaaaga atctctgaaa cgtgatgatt tcaatcatct tttatttgat actttcaatc
     8041 tcagtgatat tagtcagatc cgagatatat ttaatcaagt tgaactcaac acttgtttct
     8101 ttgaggatga aatgcagcgt aaacatgttc cgccgtgggt atatgcgcga gcgcttgatc
     8161 ctgaaacgtt gaaacctgta cctgatggaa cgccggggtt gatgagttat atggatgcgt
     8221 cagcaaccag ttatccagca tttattgtta ccgatgatgt cgggataatt agcagagaat
     8281 atggtaagta tcccggcgtg ctcgttgaaa ttttacgtcg cgtcaatacg aggacgcaga
     8341 aagggtgtgc tttaagctta accgaagcgt ttgatagttg atgcggccgc gggccccatg
     8401 gatcgatagc tggtcgacac aatctgccct ttcgaaagat cccaacgaaa agcgtgacca
     8461 catggtcctt cttgagtttg taactgctgc tgggattaca catggcatgg atgagctcta
     8521 caaataatga gctagcccgc ctaatgagcg ggcttttttt tctcggccta ggtgccgagg
     8581 atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac
     8641 tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattacaa
     8701 cttatatcgt atggggctga cttcaggtgc tacatttgaa gagataaatt gcactgaaat
     8761 ctagaaatat tttatctgat taataagatg atcttcttga gatcgttttg gtctgcgcgt
     8821 aatctcttgc tctgaaaacg aaaaaaccgc cttgcagggc ggtttttcga aggttctctg
     8881 agctaccaac tctttgaacc gaggtaactg gcttggagga gcgcagccac caaaacttgt
     8941 cctttcagtt tagccttaac cggcgcatga cttcaagact aactcctcta aatcaattac
     9001 cagtggctgc tgccagtggt gcttttgcat gtctttccgg gttggactca agacgatagt
     9061 taccggataa ggcgcagcgg tcggactgaa cggggggttc gtgcatacag tccagcttgg
     9121 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg cggccataac
     9181 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccgcca
     9241 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt
     9301 cagatttcgt gatgcttgtc aggggggcgg agcctatgga aaaacggctt tgccgcggcc
     9361 ctctcacttc cctgttaagt atcttcctgg catcttccag gaaatctccg ccccgttcgt
     9421 aagccatttc cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag
     9481 cggaatatat cctgtatcac atattctgct gacgcaccgg tgcagccttt tttctcctgc
     9541 cacatgaagc acttcactga caccctcatc agtgccaaca tagtaagcca gtatacactc
     9601 cgctagcgct gatgtccggc ggtgcttttg ccgttacgca ccaccccgtc agtagctgaa
     9661 caggagggac agtaagaggt tccaactttc accataatga aataagatca ctaccgggcg
     9721 tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
     9781 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     9841 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggacattacg gcctttttaa
     9901 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     9961 tgatgaatgc tcatccggaa ttt
//
