LOCUS       pTFGat101.1\gw3        10979 bp    DNA     circular      6-AUG-2009
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|482429637|
COMMENT     VNTDBDATE|525869756|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pTFGat101.1 gw3|
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FEATURES             Location/Qualifiers
     CDS             complement(209..514)
                     /invitrogen="1060000"
                     /vntifkey="4"
                     /label=ccdB
     CDS             complement(856..1515)
                     /invitrogen="1080000"
                     /vntifkey="4"
                     /label=Cm(R)
     primer          complement(1757..1772)
                     /invitrogen="1980000"
                     /vntifkey="27"
                     /label=M13\(-20)\forward\primer
     primer          complement(1776..1792)
                     /invitrogen="2000000"
                     /vntifkey="27"
                     /label=M13\(-40)\forward\primer
     primer          9..25
                     /invitrogen="2010000"
                     /vntifkey="27"
                     /label=M13\reverse\primer
     misc_recomb     45..169
                     /invitrogen="2702000"
                     /vntifkey="86"
                     /label=attR4
     misc_recomb     complement(1624..1748)
                     /invitrogen="2701000"
                     /vntifkey="86"
                     /label=attR3
     enhancer        complement(10138..10282)
                     /vntifkey="9"
                     /label=TEV\enhancer
     misc_feature    2272..5989
                     /vntifkey="21"
                     /label=pVS1
     misc_feature    6085..7209
                     /vntifkey="21"
                     /label=pBR322
     exon            complement(9562..10137)
                     /vntifkey="61"
                     /label=bar\ORF
     promoter        complement(7210..8450)
                     /vntifkey="30"
                     /label=aadA
     misc_feature    8455..8775
                     /vntifkey="21"
                     /label=LB
     terminator      9009..9560
                     /vntifkey="43"
                     /label=Tvsp
     insertion_seq   2130..2154
                     /vntifkey="14"
                     /label=RB
     insertion_seq   8695..8719
                     /vntifkey="14"
                     /label=L25
     promoter        complement(10283..2)
                     /vntifkey="29"
                     /label=2\X\P35S
BASE COUNT     2622 a      2893 c      2921 g      2543 t 
ORIGIN
        1 gcaagcttca ggaaacagct atgaccatga ttacgccaag ctatcaactt tgtatagaaa 
       61 agttgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa 
      121 aaaacagact acataatact gtaaaacaca acatatccag tcactatggt cgacctgcag 
      181 actggctgtg tataagggag cctgacattt atattcccca gaacatcagg ttaatggcgt 
      241 ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata acggagaccg 
      301 gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg 
      361 ggtaaagttc acgggggact ttatctgaca gcagacgtgc actggccagg gggatcacca 
      421 tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc 
      481 tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt ctcgtcggca 
      541 aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct 
      601 ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct taccgaaccg 
      661 gagatattga catcatatat gccttgagca actgatagct gtcgctgtca actgtcactg 
      721 taatacgctg cttcatagca tacctctttt tgacatactt cgggtataca tatcagtata 
      781 tattcttata ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg cttttagtaa 
      841 gccggatcct ctagattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt 
      901 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg 
      961 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa 
     1021 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga 
     1081 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca 
     1141 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca 
     1201 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc 
     1261 ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag 
     1321 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt 
     1381 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg 
     1441 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt 
     1501 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgacggat cctaactcaa 
     1561 aatccacaca ttatacgagc cggaagcata aagtgtaaag cctgggggtg cctaatgcgg 
     1621 ccgccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa 
     1681 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa ctttattata 
     1741 catagttgat aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa acaagcttgc 
     1801 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattcg taatcatgtc 
     1861 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 
     1921 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 
     1981 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 
     2041 ccaacgcgcg gggagaggcg gtttgcgtat tggagcttga gcttggatca gattgtcgtt 
     2101 tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga 
     2161 gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt 
     2221 cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttaaag 
     2281 tactttaaag tactttaaag tactttgatc caacccctcc gctgctatag tgcagtcggc 
     2341 ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg 
     2401 cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata 
     2461 aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct 
     2521 ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc 
     2581 gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc 
     2641 gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg 
     2701 accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc 
     2761 caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg 
     2821 gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc 
     2881 atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc 
     2941 cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc 
     3001 cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca 
     3061 cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag 
     3121 gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa 
     3181 gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg 
     3241 cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacgtctca accgtgcggc 
     3301 tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg 
     3361 ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct 
     3421 tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga 
     3481 acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc 
     3541 aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga 
     3601 tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt 
     3661 cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt 
     3721 agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga 
     3781 cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt 
     3841 ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt 
     3901 gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta 
     3961 cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc 
     4021 cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc 
     4081 gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc 
     4141 aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg 
     4201 ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag 
     4261 gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta 
     4321 tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat 
     4381 tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga 
     4441 atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgtctgccg 
     4501 gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca 
     4561 tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc 
     4621 gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa 
     4681 gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg 
     4741 attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac 
     4801 gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt 
     4861 gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc 
     4921 gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc 
     4981 catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg 
     5041 ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag 
     5101 aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt 
     5161 acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc 
     5221 cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct 
     5281 gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc 
     5341 gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc 
     5401 gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc 
     5461 ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg 
     5521 gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc 
     5581 aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa 
     5641 attgccctag caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt 
     5701 gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac 
     5761 attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc 
     5821 gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg 
     5881 tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc 
     5941 ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc 
     6001 ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg 
     6061 cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca 
     6121 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 
     6181 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg 
     6241 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga 
     6301 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg 
     6361 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 
     6421 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 
     6481 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 
     6541 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 
     6601 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 
     6661 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 
     6721 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 
     6781 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 
     6841 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 
     6901 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 
     6961 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 
     7021 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 
     7081 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 
     7141 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 
     7201 ttggtcatgc atgatatatc tcccaatttg tgtagggctt attatgcacg cttaaaaata 
     7261 ataaaagcag acttgacctg atagtttggc tgtgagcaat tatgtgctta gtgcatctaa 
     7321 cgcttgagtt aagccgcgcc gcgaagcggc gtcggcttga acgaatttct agctagacat 
     7381 tatttgccga ctaccttggt gatctcgcct ttcacgtagt ggacaaattc ttccaactga 
     7441 tctgcgcgcg aggccaagcg atcttcttct tgtccaagat aagcctgtct agcttcaagt 
     7501 atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc gacatccttc 
     7561 ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag cactacattt 
     7621 cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc atttagcgcc 
     7681 tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg acctaccaag 
     7741 gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc gatcgtggct 
     7801 ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgc 
     7861 ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg 
     7921 cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct 
     7981 cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt 
     8041 ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg 
     8101 agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc 
     8161 gcttccccca tgatgtttaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg 
     8221 ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga 
     8281 ggcatagact gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg 
     8341 ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgacggcag ttacgctact 
     8401 tgcattacag cttacgaacc gaacgaggct tatgtccact gggttcgtgc ccgaattgat 
     8461 cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc 
     8521 gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa 
     8581 agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt 
     8641 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag 
     8701 gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt 
     8761 tttaatgtac tgaattaacg ccgaattgct ctagcattcg ccattcaggc tgcgcaactg 
     8821 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg 
     8881 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac 
     8941 gacggccagt gccaagctaa ttcgcttcaa gacgtgctca aatcactatt tccacacccc 
     9001 tatatttcta ttgcactccc ttttaactgt tttttattac aaaaatgccc tggaaaatgc 
     9061 actccctttt tgtgtttgtt tttttgtgaa acgatgttgt caggtaattt atttgtcagt 
     9121 ctactatggt ggcccattat attaatagca actgtcggtc caatagacga cgtcgatttt 
     9181 ctgcatttgt ttaaccacgt ggattttatg acattttata ttagttaatt tgtaaaacct 
     9241 acccaattaa agacctcata tgttctaaag actaatactt aatgataaca attttctttt 
     9301 agtgaagaaa gggataatta gtaaatatgg aacaagggca gaagatttat taaagccgcg 
     9361 gtaagagaca acaagtaggt acgtggagtg tcttaggtga cttacccaca taacataaag 
     9421 tgacattaac aaacatagct aatgctccta tttgaatagt gcatatcagc ataccttatt 
     9481 acatatagat aggagcaaac tctagctaga ttgttgagca gatctcggtg acgggcagga 
     9541 ccggacgggg cggtaccggc aggctgaagt ccagctgcca gaaacccacg tcatgccagt 
     9601 tcccgtgctt gaagccggcc gcccgcagca tgccgcgggg ggcatatccg agcgcctcgt 
     9661 gcatgcgcac gctcgggtcg ttgggcagcc cgatgacagc gaccacgctc ttgaagccct 
     9721 gtgcctccag ggacttcagc aggtgggtgt agagcgtgga gcccagtccc gtccgctggt 
     9781 ggcgggggga gacgtacacg gtcgactcgg ccgtccagtc gtaggcgttg cgtgccttcc 
     9841 aggggcccgc gtaggcgatg ccggcgacct cgccgtccac ctcggcgacg agccagggat 
     9901 agcgctcccg cagacggacg aggtcgtccg tccactcctg cggttcctgc ggctcggtac 
     9961 ggaagttgac cgtgcttgtc tcgatgtagt ggttgacgat ggtgcagacc gccggcatgt 
    10021 ccgcctcggt ggcacggcgg atgtcggccg ggcgtcgttc tgggctcatg gtagatcccc 
    10081 cgttcgtaaa tggtgaaaat tttcagaaaa ttgcttttgc tttaaaagaa atgatttaaa 
    10141 ttgctgcaat agaagtagaa tgcttgattg cttgagattc gtttgttttg tatatgttgt 
    10201 gttgagaatt aattctcgag gtcctctcca aatgaaatga acttccttat atagaggaag 
    10261 ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc 
    10321 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg 
    10381 gtgggggtcc atctttggga ccactgtcgg tagaggcatc ttgaacgata gcctttcctt 
    10441 tatcgcaatg atggcatttg taggagccac cttccttttc cactatcttc acaataaagt 
    10501 gacagatagc tgggcaatgg aatccgagga ggtttccgga tattaccctt tgttgaaaag 
    10561 tctcaattgc cctttggtct tctgagactg tatctttgat atttttggag tagacaagtg 
    10621 tgtcgtgctc caccatgtta tcacatcaat ccacttgctt tgaagacgtg gttggaacgt 
    10681 cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag 
    10741 aggcatcttc aacgatggcc tttcctttat cgcaatgatg gcatttgtag gagccacctt 
    10801 ccttttccac tatcttcaca ataaagtgac agatagctgg gcaatggaat ccgaggaggt 
    10861 ttccggatat taccctttgt tgaaaagtct caattgccct ttggtcttct gagactgtat 
    10921 ctttgatatt tttggagtag acaagtgtgt cgtgctccac catgttgacc tgcaggcat 
//
