LOCUS       Exported               14979 bp ds-DNA     circular SYN 01-JUN-2021
DEFINITION  CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer
            with BsmBI sites - inserts at Neut5L - Recyclable for serial 
            mutagenesis (Sap2-FLP).
ACCESSION   .
VERSION     .
KEYWORDS    pV1393
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14979)
  AUTHORS   Vyas VK, Bushkin GG, Bernstein DA, Getz MA, Sewastianik M, Barrasa 
            MI, Bartel DP, Fink GR
  TITLE     New CRISPR Mutagenesis Strategies Reveal Variation in Repair 
            Mechanisms among Fungi.
  JOURNAL   mSphere. 2018 Apr 25;3(2). pii: 3/2/e00154-18. doi: 
            10.1128/mSphere.00154-18. Print 2018 Apr 25.
  PUBMED    29695624
REFERENCE   2  (bases 1 to 14979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 1, 2021 from SnapGene 5.1.7
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mSphere. 
            2018 Apr 25"; volume: "3(2). pii: 3/2/e00154-18. doi"; pages: " 
            10.1128/mSphere.00154-18. Print 2018 Apr 25"
FEATURES             Location/Qualifiers
     source          1..14979
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          141..4391
                     /note="/dnas_title=pV1524.no.extra.stuff"
                     /note="color: #ffffff"
     gene            141..4391
                     /label=Ca-recoded-Cas9-3xNLSSPrY
                     /note="Ca-recoded-Cas9-3xNLS"
                     /note="color: #a6acb3; direction: RIGHT"
     exon            4254..4325
                     /label=SV NLS seq 3x
                     /note="3x SV40 NLS"
                     /note="/translation=DPKKKRKVDPKKKRKVDPKKKRKV"
                     /note="color: #708090"
     misc_feature    4326..4391
                     /label=3xFLAG
                     /note="3xFLAG"
                     /note="color: #a6acb3"
     CDS             4326..4391
                     /codon_start=1
                     /gene="CBP-ProtA"
                     /product="CBP-TEV-ProteinA"
                     /label=CBP-ProtA
                     /note="fusion protein containing the followingcomponents: 
                     2218..2293 calmodulin binding protein 2321..2341 TEV 
                     cleavage site; cleavage between Q and G 2381..2728 
                     ProteinA; tandem IgG binding domain"
                     /note="/dnas_title=FLAGx3-TEV-ProteinA"
                     /note="color: #993366"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      4456..4703
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
                     /note="color: #ffffff"
     primer_bind     complement(4472..4490)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
                     /note="color: black; sequence: GCGTGAATGTAAGCGTGAC"
     primer_bind     complement(4724..4740)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(4724..4740)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
                     /note="color: black; sequence: TCTAGAACTAGTGGATC"
     CDS             5819..7090
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /note="color: #993366"
                     /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     terminator      7517..7714
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
                     /note="color: #ffffff"
     promoter        complement(8315..8658)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
                     /note="color: #ffffff; direction: LEFT"
     misc_RNA        9727..9802
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
                     /note="color: #00ccff; direction: RIGHT"
     protein_bind    complement(9882..9915)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
                     /note="color: #31849b; direction: LEFT"
     promoter        complement(10282..10300)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(10321..10337)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(10321..10337)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     primer_bind     complement(10334..10356)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGG"
     protein_bind    10345..10361
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(10369..10399)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 10369 .. 10375 / #ffffff / -10
                        2: 10376 .. 10393 / #ffffff
                        3: 10394 .. 10399 / #ffffff / -35"
     protein_bind    10414..10435
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     primer_bind     complement(10552..10569)
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     rep_origin      complement(10723..11311)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(10803..10822)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
     CDS             complement(11482..12342)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 11482 .. 12273 / #ccffcc
                        2: 12274 .. 12342 / #ccffcc / signal sequence
                     Cleavage site after base 12273"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12105..12124
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: black; sequence: ATAATACCGCGCCACATAGC"
     promoter        complement(12343..12447)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(12473..12928)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     complement(12610..12631)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
                     /note="color: black; sequence: GTGGACTCTTGTTCCAAACTGG"
     primer_bind     12822..12841
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
                     /note="color: black; sequence: AGGGAAGAAAGCGAAAGGAG"
     primer_bind     13055..13077
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
                     /note="color: black; sequence: CCCAGTCACGACGTTGTAAAACG"
     primer_bind     13069..13086
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
                     /note="color: black; sequence: TGTAAAACGACGGCCAGT"
     primer_bind     13070..13086
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     13096..13115
                     /label=T7
                     /note="T7 promoter, forward primer"
                     /note="color: black; sequence: TAATACGACTCACTATAGGG"
     promoter        13096..13114
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    complement(13567..13600)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
                     /note="color: #31849b; direction: LEFT"
ORIGIN
        1 tccaattaat ataaaataag tctgattttc caattcctgt tttttctttt tttttctgtt
       61 tctatttctt tccttttctc cctttttttt aattcttcat tcaatcatca attgataatt
      121 caggaatatt acaacccggg atggataaaa agtatagtat tggtttagat attggtacta
      181 actctgtggg ttgggcagtt atcaccgacg aatataaagt tccatcaaag aaatttaagg
      241 tgttaggtaa cactgacaga cactcaataa aaaagaatct tatcggtgct cttttgttcg
      301 actccggtga aactgccgag agaacacgtt taaaaagaac agcaagaaga agatataccc
      361 gtagaaaaaa tagaatatgt tatttacaag aaatcttttc taatgaaatg gctaaagttg
      421 atgattcctt tttccataga ttggaagagt catttttggt tgaagaagac aaaaagcatg
      481 agagacatcc aatctttggg aatatagttg atgaagtggc ttaccatgaa aaatatccta
      541 ccatttatca tttaagaaag aaattggtag attcaactga taaagctgac cttagattaa
      601 tctatttagc acttgcccat atgattaaat ttagaggtca ttttttgatt gaaggtgatt
      661 tgaacccaga taattctgac gtggataaat tatttattca attagtccaa acctacaacc
      721 aattatttga ggaaaatcca attaatgcta gtggtgtcga tgccaaagct atattatcag
      781 ccagattatc aaaatctaga cgtttggaaa atttgattgc ccaattgcca ggagaaaaaa
      841 agaatggatt atttggaaac ttgatcgcat tatcattggg tttgacacca aattttaaat
      901 ctaattttga tttagctgaa gatgctaaat tacaattatc aaaagacacc tatgacgacg
      961 atttggacaa tttacttgct caaattggtg atcaatatgc agatttgttc ttagctgcta
     1021 aaaacttatc tgatgctatt ttgttgtctg atattttgag agtgaacaca gaaataacca
     1081 aagctccatt atcagcatct atgatcaaac gttatgatga acaccatcag gatttgactt
     1141 tattgaaagc tttggtgaga caacaattgc cagagaagta taaagaaatc tttttcgatc
     1201 aatctaaaaa cgggtatgca ggttatattg atgggggtgc ctcccaagag gaattttaca
     1261 aatttataaa acctatttta gaaaagatgg atgggactga ggaacttttg gtcaaattga
     1321 acagagaaga tttgttacgt aaacagagaa cttttgataa tggtagtata cctcaccaaa
     1381 ttcatttggg tgagttgcat gcaattttaa gaagacaaga agatttttat ccatttttaa
     1441 aagataatag agaaaaaatc gagaaaattt taacctttag aattccatac tatgttgggc
     1501 ctttggctag aggtaattca agatttgcct ggatgacacg taaatcagaa gaaactatta
     1561 ccccttggaa ttttgaagag gttgttgata aaggagcatc agcacagagt tttattgaaa
     1621 gaatgaccaa tttcgataaa aacttaccaa atgaaaaagt tttaccaaaa cattccttgt
     1681 tatacgaata ttttactgtt tacaatgaac ttacaaaggt taaatatgtt actgaaggta
     1741 tgcgtaagcc agccttttta tctggagaac agaaaaaggc aatagttgat ttattgttta
     1801 aaacaaatag aaaagttact gttaaacaat taaaagaaga ttactttaag aaaattgaat
     1861 gttttgattc agttgaaatc agtggtgttg aagacagatt taatgctagt ttaggaactt
     1921 accatgattt acttaaaatt atcaaagata aagatttctt ggataacgaa gaaaatgaag
     1981 acattttaga agacattgtt ttaaccttaa ctttattcga agatagagag atgattgaag
     2041 aacgtttgaa gacttatgca catttgtttg acgataaagt gatgaaacag ttgaaaagaa
     2101 gacgttatac tggatggggt agattgtctc gtaaattgat caatggaatt agagataaac
     2161 aaagtggtaa aactatcttg gactttttga aatctgacgg atttgctaat agaaatttca
     2221 tgcaattgat ccacgacgat agtttgacat ttaaagaaga catccaaaag gcccaagtga
     2281 gtgggcaagg tgattcatta catgaacata ttgcaaattt agccggatct cctgctatta
     2341 agaaagggat attacaaact gttaaagttg tggatgaatt agtgaaagta atgggaagac
     2401 ataaacctga aaacattgtc attgagatgg caagagaaaa tcaaactaca caaaaaggac
     2461 agaaaaatag tagagaacgt atgaaaagaa tagaagaggg tattaaagaa ttgggtagtc
     2521 aaatattgaa agaacaccca gtggaaaata cccagttgca aaatgaaaaa ttatatcttt
     2581 actaccttca aaatggacgt gatatgtatg ttgatcagga attagatata aatagacttt
     2641 cagattatga tgtagatcat atagttccac aatctttctt gaaagatgat tccatagaca
     2701 ataaagtatt aactagaagt gataaaaata gaggtaaaag tgataatgtc ccaagtgagg
     2761 aagtcgtcaa aaagatgaaa aattactggc gtcaactttt gaatgctaaa ttaattactc
     2821 aaagaaaatt tgataatttg actaaagcag aaagaggtgg gctttctgaa ttagataaag
     2881 ccgggttcat taaaagacaa ttggtcgaaa ctagacaaat tactaaacat gttgcccaaa
     2941 ttttagattc ccgtatgaac actaagtatg acgaaaatga taagttaata cgtgaggtta
     3001 aagtcattac tttaaaatca aaacttgtct ctgatttcag aaaggatttc caattctata
     3061 aagttagaga aattaataat tatcatcatg ctcatgatgc atatttgaat gctgtagttg
     3121 gaactgcttt aatcaagaaa taccctaaat tagaatctga atttgtatat ggtgattaca
     3181 aagtctatga tgttagaaag atgattgcta aatcagaaca agaaattggt aaagctacag
     3241 ctaaatactt cttttactct aacattatga atttctttaa aacagaaatt actttggcaa
     3301 acggtgaaat tagaaaaaga cctcttattg aaacaaatgg tgagactgga gagatagttt
     3361 gggacaaagg gcgtgatttc gctactgtta gaaaagtttt atcaatgcca caagttaaca
     3421 ttgtaaagaa aacagaggtt caaactggtg gtttctcaaa agaaagtatt agacctaaaa
     3481 gaaatagtga taaattgatt gccagaaaaa aggattggga tccaaagaaa tatggtggtt
     3541 tcttatggcc aaccgtagcc tattctgttt tggttgtggc aaaggttgaa aagggtaaaa
     3601 gtaaaaagct taaatcagta aaagaacttt tgggtattac aataatggaa agaagttcct
     3661 ttgaaaagaa ccctattgat tttttggaag ctaaaggtta taaggaagta aagaaggact
     3721 taataatcaa attgcctaaa tattctttat ttgaattaga aaatgggaga aaaagaatgt
     3781 tggcttctgc taaacaattg caaaagggta atgaattagc attgccttcc aaatatgtta
     3841 acttcttgta tttagcttca cactatgaaa agttgaaagg gtcaccagaa gataacgagc
     3901 aaaaacaatt atttgttgaa caacacaaac actacttaga tgagattata gaacaaatta
     3961 gtgaattcag taaaagagtg atattagctg atgcaaattt agataaagtt ttgtcagcct
     4021 ataacaaaca tagagataag ccaattagag aacaagcaga aaacattatt cacttattta
     4081 cccttaccag attaggagca cctagagctt tcaagtattt tgatacaaca attgatccaa
     4141 aacaatatag atcaacaaaa gaagtcttag acgccacctt aattcatcaa tcaatcactg
     4201 gattgtatga gacaagaatt gatttgtctc aattgggtgg tgatgaaggg gctgatccta
     4261 agaagaaaag aaaagttgat ccaaagaaaa agcgtaaggt ggatcctaag aaaaagagaa
     4321 aggttgacta caaagaccat gacggtgatt ataaagatca tgacatcgac tacaaggatg
     4381 acgatgacaa gtgataatga ctgcagagat ccatcgacct gccgccaagc taattccggg
     4441 cgaatttctg tcgagtcatg taattagtta tgtcacgctt acattcacgc cctcccccca
     4501 catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt
     4561 tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc
     4621 tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg
     4681 gacgctcgaa ggctttaatt tgcggccggg ccccccctcg agggatccac tagttctaga
     4741 tttttgcaag catttaaata ttgccaagta aaaacttcaa attttctttc cccttggaac
     4801 tttgacttta tttttttgac agattatttt gacacacaca caccaaatgt gttacccctt
     4861 aaaacaaaaa aacacttttt tacaatttct tggtatccag aatcattcta agcatcattc
     4921 aattataatt tcaatccaaa aaagtagttt tagtttgact tgaaacgtca acaaacacaa
     4981 atttcaaatc ataacctctc ctgttgcctg tcaacaacac accataagga gaaggaatag
     5041 gaggaggagg agatagaaac ttgcacggca ccacaaaaca caaaattgat ttcaaccaat
     5101 acggtgacaa caacaataga tttccgatag aaataatgat tatcggaata agctagcttt
     5161 gctttgcttt gctttgcttt ttgacttgct ctaatttttc gaaaataata atggagaaaa
     5221 gttcaaggtg tttaatgcat caactaaaac agaaaataat acattagact aaacttttaa
     5281 tctttctagt accaataatt cacgcgtgcg ttttaatccc aatcatgaaa tgaagaagtt
     5341 atttcccttt ttctttcatc aaaaaagaac taaattattt tttaaatttt agtaaacaaa
     5401 acctggaaat cggggaaacc gggggagggg ggcagaaggt gaaacgggta atattgataa
     5461 atttaatcta taattgataa agttaaattt aaattgattt gaattgattt gaattgaatg
     5521 aaatgcattt gaataaacgg catcaaacta aaaaaatata gatcacattc atagtaaaac
     5581 gataacaaag aacaccacaa tttatagcaa tgataataaa catctaaaaa gaaaagggta
     5641 cgagaaggag aatgaaaaaa aacaataagc tagttcttaa tctgttcaga tatctaattt
     5701 caaaaaaaag aatagtataa aaggatagtt gattcctctt ggttgttgaa aatttgaatg
     5761 atatcaatca attaatcaat caaataacaa caacccacta gacatcacca ttgtcgacat
     5821 gccacaattt gatatattat gtaaaacacc acctaaggtg cttgttcgtc agtttgtgga
     5881 aaggtttgaa agaccttcag gtgagaaaat agcattatgt gctgctgaac taacctattt
     5941 atgttggatg attacacata acggaacagc aatcaagaga gccacattca tgagctataa
     6001 tactatcata agcaattcgt tgagtttcga tattgtcaat aaatcactcc agtttaaata
     6061 caagacgcaa aaagcaacaa ttttggaagc ctcattaaag aaattgattc ctgcttggga
     6121 atttacaatt attccttact atggacaaaa acatcaatct gatatcactg atattgtaag
     6181 tagtttgcaa ttacagttcg aatcatcgga agaagcagat aaggggaata gccacagtaa
     6241 aaaaatgctt aaagcacttc taagtgaggg tgaaagcatc tgggagatca ctgagaaaat
     6301 actaaattcg tttgagtata cttcgagatt tacaaaaaca aaaactttat accaattcct
     6361 cttcctagct actttcatca actgtggaag attcagcgat attaagaacg ttgatccgaa
     6421 atcatttaaa ttagtccaaa ataagtattt gggagtaata atccagtgtt tagtgacaga
     6481 gacaaagaca agcgttagta ggcacatata cttctttagc gcaaggggta ggatcgatcc
     6541 acttgtatat ttggatgaat ttttgaggaa ttctgaacca gtcctaaaac gagtaaatag
     6601 gaccggcaat tcttcaagca ataaacagga ataccaatta ttaaaagata acttagtcag
     6661 atcgtacaat aaagctttga agaaaaatgc gccttattca atctttgcta taaaaaatgg
     6721 cccaaaatct cacattggaa gacatttgat gacctcattt ctttcaatga agggcctaac
     6781 ggagttgact aatgttgtgg gaaattggag cgataagcgt gcttctgccg tggccaggac
     6841 aacgtatact catcagataa cagcaatacc tgatcactac ttcgcactag tttctcggta
     6901 ctatgcatat gatccaatat caaaggaaat gatagcattg aaggatgaga ctaatccaat
     6961 tgaggagtgg cagcatatag aacagctaaa gggtagtgct gaaggaagca tacgataccc
     7021 cgcatggaat gggataatat cacaggaggt actagactac ctttcatcct acataaatag
     7081 acgcatataa gagtgaaatt ctggaaatct ggaaatctgg ttttgtattc ttgttattct
     7141 tctttttgtt attacatata taacttgtta cttttttaaa aaaatctttg tatattttat
     7201 aaatatataa aactaaattt aagaaaaaga gaaaaatgtt ttatttgaga gattgaaatt
     7261 ttacttgaat ttagcttagc ttttataaag tattattatg taaaaaaaca aaacaaatat
     7321 acatcaaaaa gttaagacta taaaatagcc acccaaggca tttctatatc ttgttgttgt
     7381 tgttttcatc ttctgtatca gaggaactta ttttattatt ttcgtcacgg gtattttctc
     7441 ttgtttgatg attcatccca ttcattccat cataaaatgt cgacactgga tggcggcgtt
     7501 agtatcgaat cgacagcagt atagcgacca gcattcacat acgattgacg catgatatta
     7561 ctttctgcgc acttaacttc gcatctgggc agatgatgtc gaggcgaaaa aaaatataaa
     7621 tcacgctaac atttgattaa aatagaacaa ctacaatata aaaaaactat acaaatgaca
     7681 agttcttgaa aacaagaatc tttttattgt cagtactgac tcgagttatt atggacatgg
     7741 catagacata tacaaagctt gttcaccatc ggaagcagta ccatcgtata aagcagtatc
     7801 caaaccacac aaagtgaaac ccattcctct ataagcatga atagctggag cattaacatt
     7861 ggtaacttcc aaccacaaat gaccagcacc tctttctctg gcgaattcag tagccaaacc
     7921 catcaaagct ctaccaacac catgacctct atgttctgga gcaacttcaa tatcttcaac
     7981 agtcaatctt ctgttccaac cagaataaga aacaacaacg aaaccagcca aatcaccatc
     8041 atcaccataa gcaacgaaag ttctagaatc tggatcacca tcttcaccag catcggattc
     8101 atcatcggat tcatcatctg ggaaaacctt agtcaatggt ggatcaactg gaacttctct
     8161 caaagtgaaa ccatcaccag tagcagtaac tctaaaaaca gtatcggtag tgaaagaacc
     8221 atccaaagct tcaatagctt cagcatcacc tggaacagaa gttctgtatc tataagcagt
     8281 atcatccaaa gtagtagaca taattgtagg atccggttgt ttatgttcgg atgtgatgtg
     8341 agaactgtat cctagcaaga ttttaaaagg aagtatatga aagaagaacc tcagtggcaa
     8401 accctaacct tttatatttc tctacagggg cgcggcgtgg ggacaattca acgcgtctgt
     8461 gaggggagcg tttccctgct cgcaggtctg cagcgaggag ccgtaatttt tgcttcgcgc
     8521 cgtgcggcca tcaaaatgta tggatgcaaa tgattataca tggggatgta tgggctaaat
     8581 gtacgggcga cagtcacatc atgcccctga gctgcgcacg tcaagactgt caaggagggt
     8641 attctgggcc tccatgtcgc tggccgggtg acccggcggg gacgaggcaa gcttgatgtg
     8701 cggccgcaag tgattagact tagtccgttc aaatcaagca caactctgtt cattgtttca
     8761 acaagaatta attcaaaaac aggttcggtg cataatttgc aaaaaaatat tgcagcttct
     8821 gtggctcgaa cacagtacct ccagatttca ggtttgaaat acttcagtct gacgctctcc
     8881 cagatgagct aaagctgcaa taagaaaacc cacgccggga ttcgaacccg gaatcctttg
     8941 attagaagtc aaaagcgata accatttcgc cacgcaggcc tacttgatgg gtttgtaaat
     9001 ggtctacttt ttcagaccta acagaaattt taatgaaagt catattctta tacaataaaa
     9061 ctgtgtcata aaagcagata ttcgactttc gtagattata taggacccaa gaactaaaat
     9121 ttaatgccat attatgcatt tttaatctgt aaaagtgttg tttccaacct atcacaagta
     9181 cgttcttgta acttgtgttt gtagggttgc aaatgaatca taacaacatc tcaacagaac
     9241 atgtatagca aagcttagta taaaatcagt gttttgagag gcaatccaag aatgtttaca
     9301 tcaaagtttc aataaatatc gaccgaaact gaaaatcttt ttaggttatt gttcactttt
     9361 ttgtaaatat ttaaacattt tttggaccta aaaaaataca agcaccaatt acgtaccaag
     9421 aagcatctaa tcaactccca gatcaccact atacatttaa aagtcattgg tcaataacta
     9481 tactcgagta ttgcctcatc aaagaaacaa tcaaatatta tagatactca ctccatcacg
     9541 tgataatttc actggtatgg aaaagtggaa aattttataa aaaaaaattt gatgcctttg
     9601 gcatagctga aacttcggcc caataggatt ggagaatatg ttttcgcagc gttcttacaa
     9661 ttaaattgtg gtggaagttc gagacttgcg taaactattt ttaatttgga gacggaattc
     9721 cgtctcgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
     9781 aaaagtggca ccgagtcggt gctttttttc tcgagttttt ttatcgagtg tttaaggata
     9841 atgataactg aagagaagaa ttagttttgc cgccaccgcg ggaagttcct atactttcta
     9901 gagaatagga acttcacgcc ggtaaacaag tggtattcaa gcacaattca gttttctgga
     9961 tctctaataa ttgcaattgc aattgcttca catactctgt ttataattca gtttctttct
    10021 cttgatgtta ctgatatatt aagttaataa ataccaataa ctttttttca aaaatcttct
    10081 attttccttt caattgatta tttaaacacg aatcgttaat aagctgtgat tgctttttct
    10141 ttttcttttt cttttctttg ttcaagtttg aaaaaagtaa aaaaaaaggt ttctcttgtt
    10201 cgatgtagaa ttcagtgggt tatagttgta atgatatatg gaataaaaag aaagaaagaa
    10261 agagagctcc agcttttgtt ccctttagtg agggttaatt tcgagcttgg cgtaatcatg
    10321 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
    10381 cgggagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
    10441 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
    10501 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
    10561 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    10621 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
    10681 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    10741 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    10801 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    10861 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    10921 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    10981 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    11041 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    11101 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    11161 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    11221 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    11281 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    11341 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    11401 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    11461 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    11521 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    11581 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    11641 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    11701 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    11761 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    11821 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    11881 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    11941 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    12001 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    12061 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    12121 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    12181 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    12241 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    12301 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    12361 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    12421 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
    12481 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
    12541 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
    12601 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
    12661 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
    12721 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
    12781 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
    12841 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
    12901 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
    12961 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
    13021 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
    13081 gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgaggcctgc atgcggtacc
    13141 atgaagaatg ctgaatcact tgataggatt tagttccatt atggtactaa gaagtaatat
    13201 tacttctatg aaaaggttga atccgagatc aaaattcatc ttttcatata ctctggagaa
    13261 aaaaaaaaaa gtaattgtag taagaatgac aagtatcagt tgagaaaaaa agtaacaaag
    13321 agaaagctcg gaggaggctc cccaaaggtt ttatcaccaa tggtggaacc actaacccaa
    13381 gaacagaaaa agcattgata ttttgaaaaa atacaatact catcttttaa cccgaacctt
    13441 ttcttgtcct ttaaaacttt tatctcatat ctgtccaaag tcatattgtc ttactacttt
    13501 ctctccttca tcgtccttcc tgccactact accactggga gtttcgttct tctcgatacg
    13561 gatcctgaag ttcctatact ttctagagaa taggaacttc attagcttta cttcctgcac
    13621 tagcagtggt tggatcaaca gaatcttcat aatcatcaaa atcgtctttt gaagaccccc
    13681 cgtttgatgt atggccctgt cttttcatca aactttttat atagttgact gaactgaggc
    13741 taaatatgtg atcatcttca ctatagacaa tctttctctt atttgcacca ccgccaccac
    13801 cagtctttga gaaattctca aaacctttta cgatattacc aagcgggctc tcttcgaaat
    13861 aatctatctc tttttgatat atcgaatcct ctagcgtggt tagctttcta gttagttctt
    13921 gcttcttaag aatttgctgg attagtttat ttttcaattc aacgtatttc tcagagtcat
    13981 ctttagattt tgatgaagat gtgcgttcat tcgctatatc cttcttggtc gtgtcttttc
    14041 gatcctcctt ggctggcact gaactcgtct tttttggcgt tgctgttcca gacagactta
    14101 tctcattaga tttggaactt gtgggtttaa catcatttgc atctttagta gacatgattg
    14161 tgtaataccg tgattgtttg ttttgaaagg tctgtcatat ttctatcaat ttcaaaacaa
    14221 aatgttcatc agaaaaaagc caaaaatgtc tcttctagct tcttagtggt gtcgcaaaat
    14281 acacaatgtc gctcaacaat ccacattccc ggcgcatagc tcaaatcaca tgactacagc
    14341 taacaattac acaaaaaaat tctcttttcg atgtagcaac tatcttcaac taaaacattt
    14401 tctccttcgg cccatgattg tcctccgggt cgacagcaag ccgttacaat tgagatggaa
    14461 agcgacctac cttcactcga taaggtgctt aattgtactt catataaatc tggcccggat
    14521 ctaaacaaat gagttccatt aagccgtggg ttctcaatta gggtttttgt ttttgattta
    14581 gaaaaaagag atcaagattt gtttacaggt gatgcctttt tttagaactt atgcgttgca
    14641 aaagttgact aacgatttct ataaggtgat ccacactaat tatacaaacg tacaaacaga
    14701 catacttttc ctgcgttcac ctgatgttgg ccagatttct ctcttcattg catagaacat
    14761 aaccacacta gggcaacaga aaaaaaaaaa aaaagtgcat cgggaagttg tgttccattc
    14821 attatatgtc tactactgca tatgagtagc ccacccacca ccaccatagt aagtttttgt
    14881 gtatgcgcgc cgtcaggtta tttcatttct gaatttttca accaccttac tccctttatt
    14941 gttgattgac aattttgctc acagtaagat cttttagac
//
