LOCUS       pTwist-FLAG-SRSF3_Full. 5365 bp ds-DNA     circular     24-OCT-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(2..589)
                     /label="ori"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             1335..2195
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      2208..2344
                     /label="rrnG terminator"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2489..2560
                     /label="5' B-globin insulator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      2565..2611
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     polyA_signal    2629..2677
                     /label="poly(A) signal"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        2714..3892
                     /label="EF-1a promoter"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     intron          2945..3883
                     /label="EF-1a intron A"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             3989..4015
                     /label="FLAG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             3989..4015
                     /label="Translation 3989-4015"
     CDS             4016..4510
                     /label="SRSF3_Full.Length_Codon.Optimized"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     CDS             4016..4510
                     /label="Translation 4016-4510"
     polyA_signal    4576..4697
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     terminator      4812..4858
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     terminator      4888..5024
                     /label="rrnG terminator"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    5069..5140
                     /label="3' B-globin insulator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
       61 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
      121 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
      181 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
      241 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
      301 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
      361 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
      421 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
      481 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
      541 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT
      601 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
      661 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA
      721 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA
      781 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCATTTA AATTTCCGAA
      841 CTCTCCAAGG CCCTCGTCGG AAAATCTTCA AACCTTTCGT CCGATCCATC TTGCAGGCTA
      901 CCTCTCGAAC GAACTATCGC AAGTCTCTTG GCCGGCCTTG CGCCTTGGCT ATTGCTTGGC
      961 AGCGCCTATC GCCAGGTATT ACTCCAATCC CGAATATCCG AGATCGGGAT CACCCGAGAG
     1021 AAGTTCAACC TACATCCTCA ATCCCGATCT ATCCGAGATC CGAGGAATAT CGAAATCGGG
     1081 GCGCGCCTGG TGTACCGAGA ACGATCCTCT CAGTGCGAGT CTCGACGATC CATATCGTTG
     1141 CTTGGCAGTC AGCCAGTCGG AATCCAGCTT GGGACCCAGG AAGTCCAATC GTCAGATATT
     1201 GTACTCAAGC CTGGTCACGG CAGCGTACCG ATCTGTTTAA ACCTAGATAT TGATAGTCTG
     1261 ATCGGTCAAC GTATAATCGA GTCCTAGCTT TTGCAAACAT CTATCAAGAG ACAGGATCAG
     1321 CAGGAGGCTT TCGCATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG
     1381 CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG
     1441 ATCAGTTGGG TGCGCGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG
     1501 AGAGTTTTCG CCCCGAAGAA CGCTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG
     1561 GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT
     1621 CTCAGAATGA CTTGGTTGAG TATTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA
     1681 CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC
     1741 TTCTGACAAC GATTGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC
     1801 ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC
     1861 GTGACACCAC GATGCCTGTA GCAATGGCAA CAACCTTGCG TAAACTATTA ACTGGCGAAC
     1921 TACTTACTCT AGCTTCCCGG CAACAGTTGA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG
     1981 GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG
     2041 GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA
     2101 TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG
     2161 CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACCGAT TCTAGGTGCA TTGGCGCAGA
     2221 AAAAAATGCC TGATGCGACG CTGCGCGTCT TATACTCCCA CATATGCCAG ATTCAGCAAC
     2281 GGATACGGCT TCCCCAACTT GCCCACTTCC ATACGTGTCC TCCTTACCAG AAATTTATCC
     2341 TTAAGATCGT TTAAACTCGA CTCTGGCTCT ATCGAATCTC CGTCGTTTCG AGCTTACGCG
     2401 AACAGCCGTG GCGCTCATTT GCTCGTCGGG CATCGAATCT CGTCAGCTAT CGTCAGCTTA
     2461 CCTTTTTGGC AGCGATCGCG GCTCCCGACA TCTTGGACCA TTAGCTCCAC AGGTATCTTC
     2521 TTCCCTCTAG TGGTCATAAC AGCAGCTTCA GCTACCTCTC AATTCAAAAA ACCCCTCAAG
     2581 ACCCGTTTAG AGGCCCCAAG GGGTTATGCT ATCAATCGTT GCGTTACACA CACAAAAAAC
     2641 CAACACACAT CCATCTTCGA TGGATAGCGA TTTTATTATC TAACTGCTGA TCGAGTGTAG
     2701 CCAGATCTCG TGAGGCTCCG GTGCCCGTCA GTGGGCAGAG CGCACATCGC CCACAGTCCC
     2761 CGAGAAGTTG GGGGGAGGGG TCGGCAATTG AACCGGTGCC TAGAGAAGGT GGCGCGGGGT
     2821 AAACTGGGAA AGTGATGTCG TGTACTGGCT CCGCCTTTTT CCCGAGGGTG GGGGAGAACC
     2881 GTATATAAGT GCACTAGTCG CCGTGAACGT TCTTTTTCGC AACGGGTTTG CCGCCAGAAC
     2941 ACAGGTAAGT GCCGTGTGTG GTTCCCGCGG GCCTGGCCTC TTTACGGGTT ATGGCCCTTG
     3001 CGTGCCTTGA ATTACTTCCA CCTGGCTGCA GTACGTGATT CTTGATCCCG AGCTTCGGGT
     3061 TGGAAGTGGG TGGGAGAGTT CGTGGCCTTG CGCTTAAGGA GCCCCTTCGC CTCGTGCTTG
     3121 AGTTGTGGCC TGGCCTGGGC GCTGGGGCCG CCGCGTGCGA ATCTGGTGGC ACCTTCGCGC
     3181 CTGTCTCGCT GCTTTCGATA AGTCTCTAGC CATTTAAAAT TTTTGATGAC CTGCTGCGAC
     3241 GCTTTTTTTC TGGCAAGATA GTCTTGTAAA TGCGGGCCAA GATCAGCACA CTGGTATTTC
     3301 GGTTTTTGGG GCCGCGGGCG GCGACGGGGC CCGTGCGTCC CAGCGCACAT GTTCGGCGAG
     3361 GCGGGGCCTG CGAGCGCGGC CACCGAGAAT CGGACGGGGG TAGTCTCAAG CTGCCCGGCC
     3421 TGCTCTGGTG CCTGGCCTCG CGCCGCCGTG TATCGCCCCG CCCTGGGCGG CAAGGCTGGC
     3481 CCGGTCGGCA CCAGTTGCGT GAGCGGAAAG ATGGCCGCTT CCCGGCCCTG CTGCAGGGAG
     3541 CACAAAATGG AGGACGCGGC GCTCGGGAGA GCGGGCGGGT GAGTCACCCA CACAAAGGAA
     3601 AAGGGCCTTT CCGTCCTCAG CCGTCGCTTC ATGTGACTCC ACGGAGTACC GGGCGCCGTC
     3661 CAGGCACCTC GATTAGTTCT CCAGCTTTTG GAGTACGTCG TCTTTAGGTT GGGGGGAGGG
     3721 GTTTTATGCG ATGGAGTTTC CCCACACTGA GTGGGTGGAG ACTGAAGTTA GGCCAGCTTG
     3781 GCACTTGATG TAATTCTCCT TGGAATTTGC CCTTTTTGAG TTTGGATCTT GGTTCATTCT
     3841 CAAGCCTCAG ACAGTGGTTC AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAAAACTACC
     3901 CCTAAAAGCC AAAAGATCTT TGTCGATCCT ACCATCCACT CGACACACCC GCCAGCGGCC
     3961 GCTGCCAAGC TTCCGAGCTC TCGAATTCAT GGACTACAAA GATGACGACG ATAAAATGCA
     4021 CAGGGACTCC TGCCCCCTTG ACTGCAAGGT TTACGTGGGC AACCTTGGAA ATAATGGTAA
     4081 CAAGACAGAA CTCGAACGGG CCTTCGGCTA TTATGGACCC TTGCGCTCTG TATGGGTCGC
     4141 GAGAAATCCT CCAGGCTTTG CATTCGTAGA ATTTGAAGAT CCACGAGACG CGGCGGACGC
     4201 AGTTCGAGAG CTGGACGGTC GAACATTGTG CGGATGTCGC GTGAGGGTTG AACTTTCTAA
     4261 CGGTGAAAAG CGGAGCCGCA ATCGGGGTCC ACCACCCAGT TGGGGCAGAC GCCCTCGCGA
     4321 CGATTACCGC CGCAGAAGTC CTCCACCTAG GAGGCGATCC CCCCGACGAC GGTCATTCTC
     4381 TAGGTCTCGG AGCCGCTCCC TTAGCCGAGA CCGACGGAGG GAGAGATCCC TGTCAAGGGA
     4441 GAGGAATCAC AAACCCTCAC GCAGTTTTTC CCGGAGCAGG AGCCGATCTC GATCTAATGA
     4501 ACGAAAGTAG GGATCCGCAG GCCTCTGCTA GCTTGACTGA CTGAGATACA GCGTACCTTC
     4561 AGCTCACAGA CATGATAAGA TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT
     4621 GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA
     4681 GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG
     4741 AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATT GGCCCATCTC
     4801 TATCGGTATC GTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGTG
     4861 CCCCTCGGGC CGGATTGCTA TCTACCGGCA TTGGCGCAGA AAAAAATGCC TGATGCGACG
     4921 CTGCGCGTCT TATACTCCCA CATATGCCAG ATTCAGCAAC GGATACGGCT TCCCCAACTT
     4981 GCCCACTTCC ATACGTGTCC TCCTTACCAG AAATTTATCC TTAAGGTCGT CAGCTATCCT
     5041 GCAGGCGATC TCTCGATTTC GATCAAGACA TTCCTTTAAT GGTCTTTTCT GGACACCACT
     5101 AGGGGTCAGA AGTAGTTCAT CAAACTTTCT TCCCTCCCTA ATCTCATTGG TTACCTTGGG
     5161 CTATCGAAAC TTAATTAACC AGTCAAGTCA GCTACTTGGC GAGATCGACT TGTCTGGGTT
     5221 TCGACTACGC TCAGAATTGC GTCAGTCAAG TTCGATCTGG TCCTTGCTAT TGCACCCGTT
     5281 CTCCGATTAC GAGTTTCATT TAAATCATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC
     5341 GTAAAAAGGC CGCGTTGCTG GCGTT
//