LOCUS       Exported               10875 bp ds-DNA     circular SYN 07-JUN-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 7, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10875
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(1..372)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /note="color: #0000ff"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     primer_bind     1..18
                     /label=oriT rev Gibson
                     /note="color: green; sequence: 
                     ctgacttccgcctgcagggcTCATGGCTCTGCCCTCGG"
     oriT            405..514
                     /note="incP origin of transfer"
                     /note="color: #008000"
     primer_bind     459..477
                     /label=pCM rev Gibson
                     /note="color: green; sequence: GGACAGTGAAGAAGGAACA"
     primer_bind     complement(498..516)
                     /label=oriT fw Gibson
                     /note="color: gray; sequence: CCGGGCAGGATAGGTGAAG"
     primer_bind     complement(647..665)
                     /label=dgly GA UNS2 fw
                     /note="Su pCMU-4"
                     /note="color: black; sequence: 
                     GCTGGGAGTTCGTAGACGGAAACAAACGCAGAATCCAAGCgtgcaggtcgacggatctt
                     ; added: 2020-11-12"
     primer_bind     665..685
                     /label=dgly GA UNS2 rev
                     /note="Su pCMU-4"
                     /note="color: black; sequence: 
                     GCTTGGATTCTGCGTTTGTTTCCGTCTACGAACTCCCAGCctaggtttctgcactttct
                     g; added: 2020-11-12"
     CDS             1036..2994
                     /codon_start=1
                     /label=pSG5 rep - BbsI
                     /note="color: #ff9966"
                     /translation="MPGSTGPRNGTSPGLASRARRPRNWRSMPSEDVCSVTSVRENSAS
                     TPPRPAGPKCHCGTPIVHIPGKRPKIYCSDACRKRAKRLIADQSRSVASTTTAGNPDAG
                     FSSKGKSPSTAGQSAVTGTGSTPNEGAATAGREDPRDERFARRDRHQTVSLNEAFKGCG
                     TRLTAGTAELMWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLADAGYG
                     LHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGIIGYE
                     RAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFHDALAGAWAAGVESAGGYTVSKTC
                     DRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVSKIGLELAGQNF
                     KAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLNRLIK
                     ELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGLPGVR
                     RLVESWGLVCGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANEARTE
                     ELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKLAPVL
                     QPWGRLPGDCLRVDTAVA"
     primer_bind     complement(2249..2258)
                     /label=Seq 2.1.2
                     /note="color: black; sequence: tggcttcgatgcggtcgt"
     primer_bind     3069..3086
                     /label=Amp-Kan UNS1 fw
                     /note="color: purple; sequence: gaaagccgagggtctgcc"
     primer_bind     complement(3069..3086)
                     /label=dLancI-II 1 bis fw
                     /note="color: black; sequence: 
                     CATTACTCGCATCCATTCTCAGGCTGTCTCGTCTCGTCTCggcagaccctcggctttc"
     primer_bind     complement(3069..3086)
                     /label=dgly GA UNS9 fw
                     /note="Su pCMU-4"
                     /note="color: black; sequence: 
                     GTTCCTTATCATCTGGCGAATCGGACCCACAAGAGCACTGggcagaccctcggctttc;
                      added: 2020-11-12"
     primer_bind     complement(3113..3130)
                     /label=oJC26
                     /note="sequencing of end of pSG5 rep in pCM4.4 etc for 
                     Smanski lab"
                     /note="color: black; sequence: CGGGATTCCAGCAGTAAC; added: 
                     2021-03-15"
     primer_bind     3477..3496
                     /label=dgly GA UNS1 rev
                     /note="Su pCMU-4"
                     /note="color: black; sequence: 
                     GAGACGAGACGAGACAGCCTGAGAATGGATGCGAGTAATGtcttcttccaaagttgaga
                     a; added: 2020-11-12"
     CDS             complement(3936..4736)
                     /codon_start=1
                     /label=aac
                     /label=aac(3)IV
                     /note="color: #ff9966"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     misc_feature    4296..4315
                     /label=Protospacer targeting pCM4.4 backbone
                     /note="color: #a6acb3"
     primer_bind     complement(4889..4909)
                     /label=pCM4.4 HA site UNS7  fw
                     /note="color: black; sequence: 
                     CAAGACGCTGGCTCTGACATTTCCGCTACTGAACTACTCGacgctcagtggaacgaaaa
                     c; added: 2019-03-08"
     primer_bind     complement(4891..4908)
                     /label=pCM4.4 HA site fw
                     /note="color: gray; sequence: acgctcagtggaacgaaa"
     primer_bind     4902..4919
                     /label=pCMU-4 UNS7 rev
                     /note="color: black; sequence: 
                     CGAGTAGTTCAGTAGCGGAAATGTCAGAGCCAGCGTCTTGtgagcgtcagaccccgta;
                      added: 2019-03-08"
     primer_bind     4909..4926
                     /label=pCM4.4 HA site rev
                     /note="color: gray; sequence: cagaccccgtagaaaaga"
     misc_feature    4940..5528
                     /label=ori
                     /note="color: #a6acb3"
     rep_origin      4940..5528
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(5452..5469)
                     /label=Amp-kan rev
                     /note="color: purple; sequence: ggcgaaacccgacaggac"
     misc_feature    5483..5564
                     /label=ColE1
                     /note="color: #5555ff"
     primer_bind     5483..5502
                     /label=Fragment seq reva
                     /note="color: black; sequence: Gcgtcgatttttgtgatgct; added:
                     2019-03-08"
     primer_bind     5483..5502
                     /label=Fragment seq rev
                     /note="color: black; sequence: gcgtcgatttttgtgatgct"
     primer_bind     5483..5502
                     /label=pCM4.4_protospacer_seqR
                     /note="color: black; sequence: GCGTCGATTTTTGTGATGCT; added:
                     2021-03-15"
     primer_bind     5512..5529
                     /label=dLancI-II 3 rev
                     /note="color: black; sequence: 
                     CTCTAACGGACTTGAGTGAGGTTGTAAAGGGAGTTGGCTCggcggagcctatggaaaa"
     primer_bind     5512..5529
                     /label=dLancI-II 2 rev
                     /note="color: black; sequence: 
                     CGACCTTGATGTTTCCAGTGCGATTGAGGACCTTCAGTGCggcggagcctatggaaaa"
     primer_bind     complement(5545..5563)
                     /label=dLancI-II 4 fw
                     /note="color: black; sequence: 
                     GAGCCAACTCCCTTTACAACCTCACTCAAGTCCGTTAGAGccaggaaccgtaaaaagg"
     misc_feature    5565..5570
                     /label=unique XbaI site
                     /note="color: #3366cc"
     terminator      5571..5620
                     /label=oop ter
                     /note="color: #3366cc"
     misc_feature    5621..5701
                     /label=gRNA-tracr
                     /note="color: #3366cc"
     primer_bind     5621..5641
                     /label=oJC21
                     /note="Amplifies the glycerol targetting protospacer and 
                     gRNA scaffold from pCRISPR-cas9 as NcoI-SnaBI fragment"
                     /note="color: black; sequence: 
                     ACGCCTACGTAAAAAAAGCACCGACTCGGTGCC; added: 2021-03-15"
     primer_bind     complement(5682..5702)
                     /label=oJC13
                     /note="color: black; sequence: 
                     gtatctgaaaggggatacgcgtctgcatcagcaggttgtgttttagagctagaaatagc
                     ; added: 2020-11-12"
     primer_bind     complement(5682..5702)
                     /label=oJC17
                     /note="Amplifies the gRNA scaffold from pKCcas9dO together 
                     with universal glycerol targetting protospacer 
                     SpeI-UNS9overlap"
                     /note="color: black; sequence: 
                     agtcctaggtataatactagtgtctgcatcagcaggttgtgttttagagctagaaatag
                     c; added: 2021-03-15"
     primer_bind     complement(5682..5702)
                     /label=oJC20
                     /note="Amplifies the glycerol targetting protospacer and 
                     gRNA scaffold from pCRISPR-cas9 as NcoI-SnaBI fragment"
                     /note="color: black; sequence: 
                     CATGCCATGGgtctgcatcagcaggttgtGTTTTAGAGCTAGAAATAGC; added: 
                     2021-03-15"
     misc_feature    5704..5709
                     /label=< BbsI
                     /note="color: #3366cc"
     misc_feature    5780..6129
                     /label=LacZ cassette
                     /note="color: #66cc99; direction: RIGHT"
     misc_feature    6151..6156
                     /label=BbsI >
                     /note="color: #3366cc"
     promoter        complement(6159..6443)
                     /label=gapdhp
                     /label=gapdhp(EL)
                     /note="color: #800000; direction: LEFT"
     primer_bind     6159..6178
                     /label=oJC14
                     /note="color: black; sequence: 
                     acaacctgctgatgcagacgcgtatcccctttcagatac; added: 2020-11-12"
     primer_bind     complement(6224..6243)
                     /label=sgRNA seq fw
                     /note="color: black; sequence: ataaggcttgcagcatctgg"
     primer_bind     complement(6426..6443)
                     /label=2 pCM4.4multiUNS2 fw
                     /note="color: green; sequence: gctgctccttcggtcgga"
     primer_bind     complement(6426..6443)
                     /label=dLancI-II 3 fw
                     /note="color: black; sequence: 
                     GCACTGAAGGTCCTCAATCGCACTGGAAACATCAAGGTCGgctgctccttcggtcgga"
     primer_bind     complement(6426..6443)
                     /label=dLancI-II 2 fw
                     /note="color: black; sequence: 
                     GCTGGGAGTTCGTAGACGGAAACAAACGCAGAATCCAAGCgctgctccttcggtcgga"
     terminator      6444..6473
                     /label=fd-ter
                     /note="color: #3366cc"
     primer_bind     6596..6613
                     /label=Cas9 rev Gibson
                     /note="color: purple; sequence: cagggagtcaaaggccgc"
     primer_bind     complement(6627..6645)
                     /label=pCM fw Gibson
                     /note="color: green; sequence: TGAGAATTCAGATCTACGC"
     CDS             complement(6643..10749)
                     /codon_start=1
                     /label=codon-optimized SpCas9
                     /note="color: #ff9966"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_RNA        complement(10750..10806)
                     /label=Theophylline riboswitch
                     /label=Riboconmutador\teofilina
                     /note="Rudolph2015"
                     /note="/vntifkey=53"
                     /note="color: #0000ff; direction: LEFT"
     primer_bind     complement(10798..10829)
                     /label=Cas9 fw Gibson pCMU2 fw
                     /note="color: green; sequence: 
                     gGAGCAACGGAGGTACGGACAATACGACTCACTATAGGTTCCGGTGATACC"
     misc_feature    10807..10828
                     /label=Constant region
                     /label=Region\constante
                     /note="Rudolph2015"
                     /note="/vntifkey=21"
                     /note="color: #0000ff"
     primer_bind     complement(10817..10875)
                     /label=pCMU-4 Gibson fw
                     /note="color: purple; sequence: 
                     CCCGTCGGCTGGAAACACTTGATCCAACTTAGGATACTTCCggtaccaatacgactcac
                     "
     primer_bind     join(10835..10875,1..18)
                     /label=pCMU-4 Gibson rev
                     /note="color: green; sequence: 
                     GGAAGTATCCTAAGTTGGATCAAGTGTTTCCAGCCGACGGGtcatggctctgccctcgg
                     "
     misc_feature    10835..10875
                     /label=D4
                     /note="color: #800000"
ORIGIN
        1 tcatggctct gccctcgggc ggaccacgcc catcatgacc ttgccaagct cgtcctgctt
       61 ctcttcgatc ttcgccagca gggcgaggat cgtggcatca ccgaaccgcg ccgtgcgcgg
      121 gtcgtcggtg agccagagtt tcagcaggcc gcccaggcgg cccaggtcgc cattgatgcg
      181 ggccagctcg cggacgtgct catagtccac gacgcccgtg attttgtagc cctggccgac
      241 ggccagcagg taggccgaca ggctcatgcc ggccgccgcc gccttttcct caatcgctct
      301 tcgttcgtct ggaaggcagt acaccttgat aggtgggctg cccttcctgg ttggcttggt
      361 ttcatcagcc atccgcttgc cctcatctgt tacgccggcg gtagccggcc agcctcgcag
      421 agcaggattc ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc
      481 gctcgcgggt gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg
      541 aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac
      601 cgaaaaaatc gctataatga ccccgaagca gggttatgca gcggaaaaga tccgtcgacc
      661 tgcacctagg tttctgcact ttctgcactg cccaacgcgg gcgcgcgtgt gcgccggcca
      721 ccgcccgggg tgtaacggaa atcgccagca gacgtagtcc ttggtgagac tccgccccaa
      781 cgccctgatc tgcgaagagg accgttcgtg gaactgatga gaccgctcta cgcgagcgcg
      841 cgcggctgta acggatcacg aggtcactcc gtccataggc cgaagaggct cacgacgagg
      901 aggtcctgac gcacaggaag ccccgcccag gcccgggcgg ggcttcttcc gacctatcaa
      961 atcccaccag caccgggcgt atcggacgtc tatcggcgcg tcaggagcga gcaggggcaa
     1021 tgaacggcca caccaatgcc aggatcaaca ggacccagaa atggcacgag cccgggattg
     1081 gcctcccggg ctcgccgccc ccgcaactgg cgcagcatgc cgagtgagga cgtttgctct
     1141 gtgacgagcg tacgagagaa cagcgcgtcg acaccaccgc gccccgccgg accgaagtgc
     1201 cactgcggca ccccgatcgt ccacatcccc gggaagcgcc ccaaaatcta ctgctcggac
     1261 gcctgccgaa agcgcgcaaa gcgtctcatt gcggaccagt cacgcagcgt agcaagcacg
     1321 actacagcgg gtaatccgga cgcgggcttt agtagtaagg gaaaatctcc gtccactgca
     1381 ggtcagagcg ctgtaactgg aacgggttct accccaaacg agggtgccgc gacagccggg
     1441 cgggaggacc cgcgcgacga gcggttcgcc cggcgggacc ggcaccagac ggtgtcgctg
     1501 aacgaggcgt tcaagggctg cgggacccgg ctcactgccg ggacggccga gctgatgtgg
     1561 aagccgggcg aggcgacgtg gggcaacacg tgccgctgca acaacgtcca cacctgcccg
     1621 tggtgcatga gccgcatcct ggccgtacgg ggctcgaacg tgcagctcgc cgccgacggt
     1681 ctcgcggacg ccgggtacgg gctgcacctt ggcacgaaca cgttgcgcca cttcgagcgc
     1741 atggcgttcg gcaccgtccg caagggaatg cggcacggcc tggtcgcggt cttgcacgac
     1801 ggctggaagg gcgcgtacgg ctcgtccggc cgccgctggc gcacgatgcg cgacgacttc
     1861 gggatcatcg gctacgagcg ggccttcgag gacaccttcg gctggggctc cggctggcac
     1921 ctgcactggc acacgctctg ggtcacccgc gaggtgctcg gcccggacgc ccaggccgcg
     1981 ttccacgacg cactcgccgg agcctgggcc gccggggtcg aatccgcggg cggctacacc
     2041 gtcagcaaga cgtgcgaccg gcccggttgc tcctgtgagg gcaagggcca cggcaccgac
     2101 gtgcgcccac tcaacggcgc ggacgccgct gacggcgatg cgggcaagca ggcccgctac
     2161 ctctacaagg acggcgacaa gaccaagggc ggcgtctcca agatcggcct ggagctggcc
     2221 ggacagaact tcaaggccgg tcgcggcgac gaccgcatgg gaccgctcga cctgggcgac
     2281 gcggcggccg ccgagctgca gcggcttcgt cggcccggcc cgttcgtcga gaagtaccgc
     2341 gagcgcgagt tcggcgtgtt ccaggtccgc aagcactacc ggtcgcagaa cctgaaccgg
     2401 ctgatcaagg aactcggcat ccagcaggac gtccgcaccg aggaggagat caccgacgac
     2461 accgagggcc tggtcgccat cgcggtcatc ccggcctaca tctggtaccg atacatcgcc
     2521 cgcgtcgccg gacgtcgcct cgacctcatc aaggtcgccg agacgtacgg cctaccgggc
     2581 gtccggcggc tcgtcgagtc ctggggcctg gtgtgtggca aggacgtcct ggacccgccc
     2641 gccccggaag cgccggccgc gccgggcgac cttgacgctg accagatgag gttcgaggtc
     2701 atgtccgagg aggaagctgc tttccgcgag gcgcgccgga aggccaacga ggcccgcacg
     2761 gaggagctgg ccgcttcgct cgaccgagtg cgtcagccga agaaggaggc gatccgcccg
     2821 acgatcagcc ttcggaagcg cctgaagccg aagccggtca ccgtcgacgt caagactccc
     2881 ccgcccggcg ccgcgtcgcc cgtgtgccgc cgctgcaagg gcaagctcgc cccggtactc
     2941 cagccctggg gccagcaccc cggagactgc ctccgcgtcg acacagcggt cgcgtagggc
     3001 cgctgaggga ccgccatgag accgccaccc cggacatccg ggtggcggtc tcatggcggt
     3061 cccggcgcga aagccgaggg tctgcctgcc gtgaggtggc cggcggacct gggttactgc
     3121 tggaatcccg cgcccagtcc gagcgtccgc cggcgctttt cacctggctc cgggtgctcg
     3181 tcgatgagca ccaggccgtc gacgacgacg cgttcggcgg gcaggagccg cggggccgac
     3241 gtcgacaacg acgtcctggt ggtggtcgac ggaccggtgc cgagtgcgcg gcgaggggtg
     3301 ctcatgccga tgagtctgaa cttctccggc gaagggttct accaagtcct gggttgtgtt
     3361 cgccttggta gcgctcctag ttggcgaggc tggtccttta ccctgatgcc ctcgggcatc
     3421 agggtaaagg accagcctcc tcacatgagt aggctgaaac agcagttcgt tccgcctctt
     3481 cttccaaagt tgagaagccg cagaatccct ttctggaggg aaaccagcgt acgtacattg
     3541 cactttccct ccaggcgagg gttttgcaga tgcttggcgg tgtgcactgt ctctagctgg
     3601 catggctttg gaaatagctc cgctcagctc cgagggcttc tctggggtgg ctcccctgag
     3661 gaacctcgac atcgatacgt cgcggtgagt tcaggctttt tcatatctca ttgcccccgg
     3721 acgagcgtct gctccgccat tcgccgtccg ccgtgccaat cggatcagcc gtccaaatgc
     3781 gggattttcg ttagtcggag gccaaacggc attgagcgtc agcatatcat cagcgagctg
     3841 aagaaagaca atccccgatc cgctccacgt gttgccccag caatcagcgc gaccttgccc
     3901 ctccaacgtc atctcgttct ccgctcatga gctcagccaa tcgactggcg agcggcatcg
     3961 cattcttcgc atcccgcctc tggcggatgc aggaagatca acggatctcg gcccagttga
     4021 cccagggctg tcgccacaat gtcgcgggag cggatcaacc gagcaaaggc atgaccgact
     4081 ggaccttcct tctgaaggct cttctccttg agccacctgt ccgccaaggc aaagcgctca
     4141 cagcagtggt cattctcgag ataatcgacg cgtaccaact tgccatcctg aagaatggtg
     4201 cagtgtctcg gcaccccata gggaaccttt gccatcaact cggcaagatg cagcgtcgtg
     4261 ttggcatcgt gtcccacgcc gaggagaagt acctgcccat cgagttcatg gacacgggcg
     4321 accgggcttg caggcgagtg aggtggcagg ggcaatggat cagagatgat ctgctctgcc
     4381 tgtggccccg ctgccgcaaa ggcaaatgga tgggcgctgc gctttacatt tggcaggcgc
     4441 cagaatgtgt cagagacaac tccaaggtcc ggtgtaacgg gcgacgtggc aggatcgaac
     4501 ggctcgtcgt ccagacctga ccacgagggc atgacgagcg tccctcccgg acccagcgca
     4561 gcacgcaggg cctcgatcag tccaagtggc ccatcttcga ggggccggac gctacggaag
     4621 gagctgtgga ccagcagcac accgccgggg gtaaccccaa ggttgagaag ctgaccgatg
     4681 agctcggctt ttcgccattc gtattgcacg acattgcact ccaccgctga tgacatcagt
     4741 cgatcatagc acgatcaacg gcactgttgc aaatagtcgg tggtgataaa cttatcatcc
     4801 ccttttgctg atggagctgc acatgaacca aaaggatcta ggtgaagatc ctttttgata
     4861 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     4921 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4981 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5041 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     5101 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5161 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5221 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5281 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5341 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5401 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5461 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5521 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggcctctaga taaaaaacgc
     5581 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt aaaaagcacc gactcggtgc
     5641 cactttttca agttgataac ggactagcct tattttaact tgctatttct agctctaaaa
     5701 catgtcttca ccggtggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     5761 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     5821 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     5881 ttaaccctca ctaaagggaa caaaagctgg aggacctcga gggggggccc ggtacccaat
     5941 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     6001 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     6061 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     6121 ggcgaatggg acgcgccctg tagcggctga gaagacttgc gtatcccctt tcagatactc
     6181 gcactaagaa ttgcaggaac gccccgatca tagcggtagc cgcccagatg ctgcaagcct
     6241 tatctggcag ccgtataaaa aaagcaaccg aacaggccat tcacagaagt ttcacaccgc
     6301 tcgccgaggg gctccgcacc cggtgcgcgc cgtacagcag cgcgcttctc ccagcatcgg
     6361 ccagttcgcc cgatccgtcc gtgtcgcaca gccgacggct gcggtaaggt gcccgtagac
     6421 gcacgtccga ccgaaggagc agcaaaaaaa ggctccaaaa ggagccttta attgtatcgg
     6481 tttatcagct tgctttcgag gtgaatttct taaacagctt gataccgata gttgcgccga
     6541 caatgacaac aaccatcgcc cacgcataac cgatatattc ggtcgctgag gcttgcaggg
     6601 agtcaaaggc cgcttttgcg gggaacgcgt agatctgaat tctcagtcgc cgccgagctg
     6661 ggagaggtcg atgcgggttt cgtacaggcc ggtgatggac tggtggatca gggtcgcgtc
     6721 gaggacctcc ttggtgctgg tgtaccgctt gcggtcgatg gtggtgtcga agtacttgaa
     6781 cgcggcgggg gcgcccaggt tggtgagggt gaacaggtgg atgatgttct ccgcctgctc
     6841 ccggatcggc ttgtcgcggt gcttgttgta ggcggacagc accttgtcga ggttcgcgtc
     6901 ggccaggatg acccgcttgc tgaactccga gatctgctcg atgatctcgt ccaggtagtg
     6961 cttgtgctgc tccacgaaga gctgcttctg ctcgttgtcc tcgggcgagc ccttcagctt
     7021 ctcgtagtgg gacgcgaggt acaggaagtt gacgtacttg gacgggaggg ccagctcgtt
     7081 gcccttctgc agctcgcccg cgctggcgag catccgcttg cggccgttct cgagctcgaa
     7141 cagcgagtac ttgggcagct tgatgatgag gtccttcttg acctccttgt agcccttggc
     7201 ctccaggaag tcgatcgggt tcttctcgaa cgaggagcgc tccatgatgg tgatgccgag
     7261 cagctccttc acggacttca gcttcttgct cttgcccttc tcgaccttcg ccacgaccag
     7321 cacggagtag gcgacggtgg ggctgtcgaa gccgccgtac ttcttcgggt cccagtcctt
     7381 cttccgggcg atcagcttgt ccgagttgcg cttggggagg atggactcct tgctgaagcc
     7441 gccggtctgc acctcggttt tcttgacgat gttgacctgc ggcatggaca gcaccttccg
     7501 gacggtggcg aagtcgcggc ccttgtccca gacgatctcg ccggtttcgc cgttggtttc
     7561 gatcaggggc cgcttgcgga tctcgccgtt ggccagggtg atctcggtct tgaagaagtt
     7621 catgatgttg ctgtagaaga agtacttcgc ggtggccttg ccgatctcct gctccgactt
     7681 ggcgatcatc ttgcgcacgt cgtagacctt gtagtcgccg tagacgaact cggactccag
     7741 cttcgggtac ttcttgatca gcgcggtgcc cacgacggcg ttcaggtacg cgtcgtgggc
     7801 gtggtggtag ttgttgatct cccggacctt gtagaactgg aagtccttgc ggaagtcgga
     7861 caccagcttg ctcttgaggg tgatcacctt gacctcgcgg atcagcttgt cgttctcgtc
     7921 gtacttggtg ttcatccggc tgtccaggat ctgggccacg tgcttggtga tctgccgggt
     7981 ttcgacgagc tggcgcttga tgaagcccgc cttgtcgagc tccgacaggc cgccccgctc
     8041 ggccttggtc aggttgtcga acttgcgctg ggtgatgagc ttggcgttga gcagctggcg
     8101 ccagtagttc ttcatcttct tcacgacctc ctccgagggc acgttgtcgg acttgccccg
     8161 gttcttgtcg gagcgggtca ggaccttgtt gtcgatcgag tcgtccttca ggaaggactg
     8221 cggcacgatg tggtcgacgt cgtagtcgct cagccggttg atgtcgagct cctggtccac
     8281 gtacatgtcg cggccgttct gcaggtagta gaggtacagc ttctcgttct ggagctgggt
     8341 gttctcgacc gggtgctcct tcaggatctg cgagccgagc tccttgatgc cctcctcgat
     8401 gcgcttcatc cgctcgcggg agttcttctg gcccttctgg gtggtctggt tctcccgggc
     8461 catctcgatc acgatgttct cgggcttgtg gcggcccatc accttgacca gctcgtccac
     8521 gaccttgacg gtctggagga tgcccttctt gatcgccggg gagcccgcca ggttggcgat
     8581 gtgctcgtgg aggctgtcgc cctggccgga cacctgggcc ttctggatgt cctccttgaa
     8641 ggtcagcgag tcgtcgtgga tgagctgcat gaagttgcgg ttggcgaagc cgtcggactt
     8701 gaggaagtcc aggatggtct tgccgctctg cttgtcccgg atgccgttga tcagcttccg
     8761 ggagaggcgg ccccagccgg tgtaccggcg ccgcttcagc tgcttcatca ccttgtcgtc
     8821 gaacaggtgg gcgtaggtct tgagccgctc ctcgatcatc tcgcggtcct cgaagagggt
     8881 cagggtgagg acgatgtcct ccaggatgtc ctcgttctcc tcgttgtcga ggaagtcctt
     8941 gtccttgatg atcttgagca ggtcgtggta ggtgcccagg gaggcgttga agcggtcctc
     9001 cacgcccgag atctcgacgg agtcgaagca ctcgatcttc ttgaagtagt cctccttcag
     9061 ctgcttcacg gtgaccttgc ggttggtctt gaagagcagg tcgacgatcg ccttcttctg
     9121 ctcgccggac aggaaggccg gcttccgcat gccctcggtc acgtacttga ccttggtcag
     9181 ctcgttgtac acggtgaagt actcgtagag caggctgtgc ttgggcagga ccttctcgtt
     9241 cgggaggttc ttgtcgaagt tggtcatgcg ctcgatgaac gactgcgcgg aggcgccctt
     9301 gtccacgacc tcctcgaagt tccacggggt gatggtttcc tccgacttcc gggtcatcca
     9361 cgcgaaccgg gagttgccgc gggccagggg gccgacgtag tacgggatcc ggaaggtcag
     9421 gatcttctcg atcttctcgc ggttgtcctt caggaagggg tagaagtcct cctggcgccg
     9481 gaggatggcg tggagctcgc ccaggtggat ctggtgcggg atggagccgt tgtcgaaggt
     9541 ccgctgcttg cggagcaggt cctcgcggtt cagcttgacg agcagctcct cggtgccgtc
     9601 catcttctcc aggatcggct tgatgaactt gtagaactcc tcctgcgacg cgccgccgtc
     9661 gatgtagccg gcgtagccgt tcttggactg gtcgaagaag atctccttgt acttctcggg
     9721 cagctgctgg cgcacgaggg ccttgagcag ggtcaggtcc tggtggtgct cgtcgtaccg
     9781 cttgatcatg ctcgccgaca gcggggcctt ggtgatctcg gtgttgaccc gcaggatgtc
     9841 gctgagcagg atggcgtccg agaggttctt cgcggccagg aagaggtccg cgtactggtc
     9901 gccgatctgg gcgagcaggt tgtccaggtc gtcgtcgtag gtgtccttgg acagctggag
     9961 cttcgcgtcc tcggccaggt cgaagttgct cttgaagttg ggggtcaggc cgagcgacag
    10021 cgcgatcagg ttgccgaaga ggccgttctt cttctcgccc ggcagctggg cgatgaggtt
    10081 ctccaggcgc cgggacttgc tcaggcgcgc ggagaggatc gccttggcgt cgacgccgct
    10141 ggcgttgatg gggttctcct cgaacagctg gttgtaggtc tgcaccagct ggatgaagag
    10201 cttgtcgacg tcggagttgt ccgggttcag gtcgccctcg atgaggaagt ggccgcggaa
    10261 cttgatcatg tgcgcgaggg ccaggtagat gagccgcagg tccgccttgt cggtcgagtc
    10321 gaccagcttc ttgcggaggt ggtagatggt ggggtacttc tcgtggtagg ccacctcgtc
    10381 gacgatgttg ccgaagatcg ggtggcgctc gtgcttcttg tcctcctcca ccaggaagct
    10441 ctcctcgagc cggtggaaga acgagtcgtc gaccttggcc atctcgttgg agaagatctc
    10501 ctgcaggtag cagatgcggt tcttgcgccg ggtgtagcgg cgacgggcgg tccgcttcag
    10561 gcgggtcgcc tcggcggttt cgccgctgtc gaagagcagg gcgccgatca ggttcttctt
    10621 gatcgagtgc cggtcggtgt tgcccaggac cttgaacttc ttggagggga ccttgtactc
    10681 gtcggtgatg accgcccagc ccacgctgtt ggtgccgatg tccaggccga tgctgtactt
    10741 cttgtccatc ttgttgcctc cttagcaggg tgctgccaag ggcatcaaga cgatgctggt
    10801 atcaccggaa cctatagtga gtcgtattgg taccggaagt atcctaagtt ggatcaagtg
    10861 tttccagccg acggg
//
